Gene expression analysis and data mining from microarray analysis applied to drought stress in rice

2009 ◽  
pp. 275-298
Author(s):  
Kouji Satoh ◽  
Koji Doi ◽  
Toshifumi Nagata ◽  
Aeni Hosaka ◽  
Kohji Suzuki ◽  
...  
Keyword(s):  
Gene Expression ◽  
Data Mining ◽  
Drought Stress ◽  
Microarray Analysis ◽  
Expression Analysis ◽  
Gene Expression Analysis

scholarly journals Antioxidant gene expression analysis and evaluation of total phenol content and oxygen-scavenging system in tea accessions under normal and drought stress conditions

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Mehdi Rahimi ◽  
Mojtaba Kordrostami ◽  
Fereshteh Mohamadhasani ◽  
Sanam Safaei Chaeikar
Keyword(s):  
Gene Expression ◽  
Antioxidant Activity ◽  
Antioxidant Enzymes ◽  
Drought Stress ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Stress Conditions ◽  
Total Phenol Content ◽  
Phenol Content ◽  
Oxygen Scavenging

Abstract Background Abiotic and biotic stresses induce oxidative processes in plant cells that this process starts with the production of ROSs which cause damage to the proteins. Therefore, plants have increased their antioxidant activity to defend against this oxidative stress to be able to handle stress better. In this research, 14 different tea accessions in a randomized complete block design with two replications were evaluated in two normal and drought stress conditions, and their antioxidant activity was measured by DPPH-free radicals’ assay and gene expression analysis. Results The results of gene expression analysis showed that the 100 and 399 accessions and Bazri cultivar had high values for most of the antioxidant enzymes, ascorbate peroxidase, superoxide dismutase, catalase, and peroxidase under drought stress conditions while the 278 and 276 accessions had the lowest amount of antioxidant enzymes in the same situation. Results showed that the IC50 of the BHT combination was 90.12 μg/ ml. Also, The IC50 of accessions ranged from 218 to 261 μg/ml and 201–264 μg/ml at normal and drought stress conditions, respectively. The 100 and 399 accessions showed the lowest IC50 under normal and drought stress conditions, while 278 and 276 accessions had the highest value for IC50. The antioxidant activity of tea accession extracts under normal conditions was ranged from 25 to 69% for accessions 278 and 100, respectively. While, the antioxidant activities of extracts under drought stress condition was 12 to 83% for accessions 276 and 100, respectively. So, according to the results, 100 and 399 accessions exhibited the least IC50 and more antioxidant activity under drought stress conditions and were identified as stress-tolerant accessions. However, 278 and 276 accessions did not show much antioxidant activity and were recognized as sensitive accessions under drought stress conditions. Conclusions These results demonstrate that total phenol content, antioxidant activity, and the oxygen-scavenging system can be used as a descriptor for identifying drought-tolerant accessions.

scholarly journals The role of dipeptidyl peptidase 4 (DPP4) in the preservation of renal function: DPP4 involvement in hemoglobin expression

2014 ◽  
Vol 223 (2) ◽  
pp. 133-142 ◽  
Author(s):  
Youichi Sato ◽  
Takanobu Kamada ◽  
Aiko Yamauchi
Keyword(s):  
Gene Expression ◽  
Renal Function ◽  
Microarray Analysis ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Dipeptidyl Peptidase ◽  
Hek293 Cells ◽  
Expression Levels ◽  
Dipeptidyl Peptidase 4

In a previous study, we demonstrated that dipeptidyl peptidase 4 (DPP4)-deficient rats were susceptible to reduced glomerular filtration rate as a result of streptozotocin (STZ)-induced diabetes. Therefore, we proposed that DPP4 might be responsible for the preservation of renal function. In this study, to verify the role of DPP4 in the preservation of renal function, we performed a microarray analysis of the kidneys of WT and DPP4-deficient rats after STZ treatment, and gene expression analysis using rat kidneys, human embryonic kidney 293 (HEK293) cells, and human renal cancer cells (CakI-1). The microarray analysis indicated that the expression levels of the transporter activity, heme-binding, and pheromone binding-related genes changed significantly. The results of gene expression analysis indicated that there were no significant differences in the expression levels of hemoglobin mRNA between the DPP4-deficient and WT rats; however, the expression levels of hemoglobin mRNA in the kidneys of DPP4-deficient rats tended to decrease when compared with those of both the non-STZ-treated and STZ-treated WT rats. The expression levels of hemoglobin in HEK293 and Caki-1 cells were significantly decreased whenDPP4was knocked down by siRNA, were significantly increased by the addition of soluble human DPP4, and were also significantly increased by the addition of the DPP4 inhibitor, sitagliptin. The expression level ofDPP4was also significantly increased by the addition of sitagliptin in both cell types. Our findings indicate that DPP4 regulates the expression of the hemoglobin genes, and might play a role in the preservation of renal function; however, the underlying mechanism of this preservation remains to be elucidated.

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