Gene expression analysis in Eucalyptus globulus exposed to drought stress in a controlled and a field environment indicates different strategies for short- and longer-term acclimation

2018 ◽  
Author(s):  
Barbara Correia ◽  
Robert D Hancock ◽  
Luis Valledor ◽  
Glória Pinto
Keyword(s):  
Gene Expression ◽  
Drought Stress ◽  
Expression Analysis ◽  
Eucalyptus Globulus ◽  
Gene Expression Analysis ◽  
Field Environment

scholarly journals Antioxidant gene expression analysis and evaluation of total phenol content and oxygen-scavenging system in tea accessions under normal and drought stress conditions

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Mehdi Rahimi ◽  
Mojtaba Kordrostami ◽  
Fereshteh Mohamadhasani ◽  
Sanam Safaei Chaeikar
Keyword(s):  
Gene Expression ◽  
Antioxidant Activity ◽  
Antioxidant Enzymes ◽  
Drought Stress ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Stress Conditions ◽  
Total Phenol Content ◽  
Phenol Content ◽  
Oxygen Scavenging

Abstract Background Abiotic and biotic stresses induce oxidative processes in plant cells that this process starts with the production of ROSs which cause damage to the proteins. Therefore, plants have increased their antioxidant activity to defend against this oxidative stress to be able to handle stress better. In this research, 14 different tea accessions in a randomized complete block design with two replications were evaluated in two normal and drought stress conditions, and their antioxidant activity was measured by DPPH-free radicals’ assay and gene expression analysis. Results The results of gene expression analysis showed that the 100 and 399 accessions and Bazri cultivar had high values for most of the antioxidant enzymes, ascorbate peroxidase, superoxide dismutase, catalase, and peroxidase under drought stress conditions while the 278 and 276 accessions had the lowest amount of antioxidant enzymes in the same situation. Results showed that the IC50 of the BHT combination was 90.12 μg/ ml. Also, The IC50 of accessions ranged from 218 to 261 μg/ml and 201–264 μg/ml at normal and drought stress conditions, respectively. The 100 and 399 accessions showed the lowest IC50 under normal and drought stress conditions, while 278 and 276 accessions had the highest value for IC50. The antioxidant activity of tea accession extracts under normal conditions was ranged from 25 to 69% for accessions 278 and 100, respectively. While, the antioxidant activities of extracts under drought stress condition was 12 to 83% for accessions 276 and 100, respectively. So, according to the results, 100 and 399 accessions exhibited the least IC50 and more antioxidant activity under drought stress conditions and were identified as stress-tolerant accessions. However, 278 and 276 accessions did not show much antioxidant activity and were recognized as sensitive accessions under drought stress conditions. Conclusions These results demonstrate that total phenol content, antioxidant activity, and the oxygen-scavenging system can be used as a descriptor for identifying drought-tolerant accessions.

Gene expression analysis and data mining from microarray analysis applied to drought stress in rice

2009 ◽  
pp. 275-298
Author(s):  
Kouji Satoh ◽  
Koji Doi ◽  
Toshifumi Nagata ◽  
Aeni Hosaka ◽  
Kohji Suzuki ◽  
...  
Keyword(s):  
Gene Expression ◽  
Data Mining ◽  
Drought Stress ◽  
Microarray Analysis ◽  
Expression Analysis ◽  
Gene Expression Analysis

scholarly journals cDNA-SRAP and Its Application in Differential Gene Expression Analysis: A Case Study inErianthus arundinaceum

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Youxiong Que ◽  
Liping Xu ◽  
Jianwei Lin ◽  
Jun Luo ◽  
Jingsheng Xu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Drought Stress ◽  
Differential Gene Expression ◽  
Expression Analysis ◽  
Drought Resistance ◽  
Gene Expression Analysis ◽  
Ammonia Monooxygenase ◽  
Differential Gene Expression Analysis ◽  
Differential Gene ◽  
Vacuolar Protein

Erianthus arundinaceumis a wild relative species of sugarcane. The aim of this research was to demonstrate the feasibility of cDNA-SRAP for differential gene expression and to explore the molecular mechanism of drought resistance inE. arundinaceum. cDNA-SRAP technique, for the first time, was applied in the analysis of differential gene expression inE. arundinaceumunder drought stress. In total, eight differentially expressed genes with length of 185–427 bp were successfully isolated (GenBank Accession numbers: EU071770, EU071772, EU071774, EU071776, EU071777, EU071779, EU071780, and EU071781). Based on their homologies with genes in GenBank, these genes were assumed to encode ribonuclease III, vacuolar protein, ethylene insensitive protein, aerobactin biosynthesis protein, photosystem II protein, glucose transporter, leucine-rich repeat protein, and ammonia monooxygenase. Real-time PCR analysis on the expression profiling of gene (EU071774) encoding ethylene-insensitive protein and gene (EU071781) encoding ammonia monooxygenase revealed that the expression of these two genes was upregulated both by PEG and ABA treatments, suggesting that they may involve in the drought resistance ofE. arundinaceum. This study constitutes the first report of genes activated inE. arundinaceumby drought stress and opens up the application of cDNA-SRAP in differential gene expression analysis inE. arundinaceumunder certain stress conditions.

Root Morphology and Gene Expression Analysis in Response to Drought Stress in Maize (Zea mays)

2011 ◽  
Vol 30 (2) ◽  
pp. 360-369 ◽  
Author(s):  
Tingbo Jiang ◽  
Jake Fountain ◽  
Georgia Davis ◽  
Robert Kemerait ◽  
Brian Scully ◽  
...  
Keyword(s):  
Gene Expression ◽  
Zea Mays ◽  
Drought Stress ◽  
Expression Analysis ◽  
Root Morphology ◽  
Gene Expression Analysis
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