IMPURITY INDUCED WRINKLING PATTERNS IN METAL FILMS DEPOSITED ON SOFT ELASTIC SUBSTRATES

2017 ◽  
Vol 24 (03) ◽  
pp. 1750034 ◽  
Author(s):  
YONG-JU ZHANG ◽  
SEN-JIANG YU ◽  
MIAO-GEN CHEN ◽  
PING-GEN CAI ◽  
HONG ZHOU

Metal (iron and nickel) films have been deposited on soft elastic polydimethylsiloxane (PDMS) substrates by direct current sputtering technique and the impurity induced wrinkling patterns are investigated by using optical microscopy and atomic force microscopy. It is found that the metal films can spontaneously form disordered wrinkles due to the isotropic compressive stress. In the vicinity of film impurities such as extraneous particles, linear defects, cracks and thickness-gradient film edges, the stress field becomes anisotropic owing to symmetry breaking and thus complex wrinkling patterns including straight stripes, herringbones, crossings, labyrinths and their transitions can be observed. The morphological evolutions, structural characteristics and physical mechanisms of the impurity induced wrinkles have been discussed and analyzed based on the continuum elastic theory.

2000 ◽  
Vol 279 (1-3) ◽  
pp. 98-101 ◽  
Author(s):  
E.Z. Luo ◽  
A.B. Pakhomov ◽  
Zhao-Qing Zhang ◽  
M.-C. Chan ◽  
I.H. Wilson ◽  
...  

2006 ◽  
Vol 252 (14) ◽  
pp. 5083-5099 ◽  
Author(s):  
Morihide Higo ◽  
Katsuya Fujita ◽  
Yuya Tanaka ◽  
Masaru Mitsushio ◽  
Toshifumi Yoshidome

2016 ◽  
Vol 74 (3) ◽  
pp. 365-371 ◽  
Author(s):  
Ying LI ◽  
Liyuan Lu ◽  
Juan LI

Abstract Hereditary spherocytosis is an inherited red blood cell membrane disorder resulting from mutations of genes encoding erythrocyte membrane and cytoskeletal proteins. Few equipments can observe the structural characteristics of hereditary spherocytosis directly expect for atomic force microscopy In our study, we proved atomic force microscopy is a powerful and sensitive instrument to describe the characteristics of hereditary spherocytosis. Erythrocytes from hereditary spherocytosis patients were small spheroidal, lacking a well-organized lattice on the cell membrane, with smaller cell surface particles and had reduced valley to peak distance and average cell membrane roughness vs. those from healthy individuals. These observations indicated defects in the certain cell membrane structural proteins such as α- and β-spectrin, ankyrin, etc. Until now, splenectomy is still the most effective treatment for symptoms relief for hereditary spherocytosis. In this study, we further solved the mysteries of membrane nanostructure changes of erythrocytes before and after splenectomy in hereditary spherocytosis by atomic force microscopy. After splenectomy, the cells were larger, but still spheroidal-shaped. The membrane ultrastructure was disorganized and characterized by a reduced surface particle size and lower than normal Ra values. These observations indicated that although splenectomy can effectively relieve the symptoms of hereditary spherocytosis, it has little effect on correction of cytoskeletal membrane defects of hereditary spherocytosis. We concluded that atomic force microscopy is a powerful tool to investigate the pathophysiological mechanisms of hereditary spherocytosis and to monitor treatment efficacy in clinical practices. To the best of our knowledge, this is the first report to study hereditary spherocytosis with atomic force microscopy and offers important mechanistic insight into the underlying role of splenectomy.


2013 ◽  
Vol 19 (3) ◽  
pp. 544-552 ◽  
Author(s):  
Zhiguo Liu ◽  
Lin Zhao ◽  
Yuangang Zu ◽  
Shengnan Tan ◽  
Yuanlin Wang ◽  
...  

AbstractIt is important to know the detailed DNA structure on carbonaceous surfaces for further application of DNA-functionalized carbonaceous materials in diverse research areas. In this study, the topographic and structural characteristics of the separated single DNA molecules and their assembly on highly oriented pyrolytic graphite (HOPG) surfaces have been investigated by atomic force microscopy (AFM). AFM results indicate that both circular and linear DNA molecules tend to form hexagonal patterns along with some unusual structures that include node, protrusion, cruciform, parallel single-stranded DNA (ssDNA), and compact zigzag. Furthermore, parallel ssDNA patterns and their crossed structures have been obtained under high-temperature conditions. Our AFM results reveal that a bare HOPG surface can induce DNA molecules to form various unusual structures. This finding is helpful for understanding the adsorption behavior of DNA on other carbonaceous surfaces such as carbon nanotubes and graphene. In addition, the hexagonal DNA patterns in this study are similar to those formed on the alkylamine-modified HOPG surface, which implies that a bare HOPG, without any chemical modification, has a strong ability to align biomolecules. This study could expand our knowledge of the diversities of DNA structures and the aligning ability of carbonaceous surfaces.


2020 ◽  
pp. 117-127
Author(s):  
Ivy Emnace

In the search for exopolysaccharide (EPS)—producing LAB strains as potential additive for industrial applications, EPS produced by Lactobacillus rhamnosus NBRC 3425 was subjected to partial characterization to provide further information on its structure and composition. The crude EPS was subjected to scanning electron microscopy (SEM), atomic force microscopy (AFM),transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). Results of TEM analysis confirmed that the test organism is an EPS producer due to the presence of an unstained, clear cell wall or halo that surrounds the bacterial cell typical of a capsular EPS. SEM analysis showed that the crude EPS has pores and spaces between particles. Atomic force microscopy (AFM) at a concentration of 10μg per mL revealed spike-shaped lumps with an average size of 17.81±2.89nm. The FTIR spectrum suggested the presence of hydroxyl (OH) groups of carbohydrate and carbonyl group (C=O). Results showed that based on its structural characteristics, such EPS has the potential for use as stabilizer in food products.


2019 ◽  
Vol 206 (3) ◽  
pp. 314-321 ◽  
Author(s):  
Yongmei Tian ◽  
Yongjun Wu ◽  
Lie Liu ◽  
Leiliang He ◽  
Jing Gao ◽  
...  

2004 ◽  
Vol 44 (supplement) ◽  
pp. S116
Author(s):  
R. Kono ◽  
R. Takeuchi ◽  
M. Fujisawa ◽  
H. Tachibana ◽  
K. Akasaka

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