GASTROINTESTINAL ABNORMALITIES IDENTIFIED BY FLUORESCENCE ENDOMICROSCOPY

Real-time in vivo microscopic imaging has become a reality with the advent of confocal and nonlinear endomicroscopy. These devices are best utilized in conjunction with standard white light endoscopy. We evaluated the use of fluorescence endomicroscopy in detecting microscopic abnormalities in colonic tissues. Mice of C57bl/6 strain had intraperitoneal injection with azoxymethane once every week for five weeks and littermates, not exposed to azoxymethane served as controls. After 14 weeks, intestines were imaged by fluorescence endomicroscopy. The images show obvious cellular structural differences between those two groups of mice. The difference in endomicroscopy imaging can be used for identifying tissues suspicious for neoplasia or other changes, leading to early diagnosis of gastrointestinal track of cancer.

Download Full-text

Comparison of In Vivo Raman Spectroscopy With White Light Endoscopy, Narrow Band Imaging, and Confocal Endomicroscopy for the Real-time Diagnosis of Barrettʼs Esophagus

The American Journal of Gastroenterology ◽

10.14309/00000434-201510001-01509 ◽

2015 ◽

Vol 110 ◽

pp. S652-S653

Author(s):

Neel Sharma ◽

Lin Kan ◽

Wang Jianfeng ◽

Bryan Choo ◽

Jin Rong Khoo ◽

...

Keyword(s):

Raman Spectroscopy ◽

Real Time ◽

White Light ◽

Narrow Band ◽

Narrow Band Imaging ◽

The Real ◽

White Light Endoscopy ◽

In Vivo Raman Spectroscopy ◽

Time Diagnosis

Download Full-text

Multi-Modal Multi-Spectral Intravital Microscopic Imaging of Signaling Dynamics in Real-Time during Tumor–Immune Interactions

Cells ◽

10.3390/cells10030499 ◽

2021 ◽

Vol 10 (3) ◽

pp. 499

Author(s):

Tracy W. Liu ◽

Seth T. Gammon ◽

David Piwnica-Worms

Keyword(s):

Molecular Imaging ◽

Single Cell ◽

Real Time ◽

Bioluminescence Imaging ◽

Emission Spectra ◽

Ccd Camera ◽

Microscopic Imaging ◽

Signaling Dynamics ◽

Window Chamber

Intravital microscopic imaging (IVM) allows for the study of interactions between immune cells and tumor cells in a dynamic, physiologically relevant system in vivo. Current IVM strategies primarily use fluorescence imaging; however, with the advances in bioluminescence imaging and the development of new bioluminescent reporters with expanded emission spectra, the applications for bioluminescence are extending to single cell imaging. Herein, we describe a molecular imaging window chamber platform that uniquely combines both bioluminescent and fluorescent genetically encoded reporters, as well as exogenous reporters, providing a powerful multi-plex strategy to study molecular and cellular processes in real-time in intact living systems at single cell resolution all in one system. We demonstrate that our molecular imaging window chamber platform is capable of imaging signaling dynamics in real-time at cellular resolution during tumor progression. Importantly, we expand the utility of IVM by modifying an off-the-shelf commercial system with the addition of bioluminescence imaging achieved by the addition of a CCD camera and demonstrate high quality imaging within the reaches of any biology laboratory.

Download Full-text

Mo1324 REAL-TIME DIAGNOSTIC YIELD OF WHITE LIGHT ENDOSCOPY, CHROMOENDOSCOPY AND MAGNIFYING ENDOSCOPY WITH NARROW BAND IMAGING IN THE UNDIAGNOSED GASTRIC LESIONS AFTER HELICOBACTER PYLORI ERADICATION

Gastrointestinal Endoscopy ◽

10.1016/j.gie.2019.03.803 ◽

2019 ◽

Vol 89 (6) ◽

pp. AB491

Author(s):

Tomomitsu Tahara ◽

Noriyuki Horiguchi ◽

Tsuyoshi Terada ◽

Dai Yoshida ◽

Masaaki Okubo ◽

...

Keyword(s):

Helicobacter Pylori ◽

Real Time ◽

White Light ◽

Narrow Band ◽

Narrow Band Imaging ◽

Diagnostic Yield ◽

Magnifying Endoscopy ◽

Gastric Lesions ◽

Helicobacter Pylori Eradication ◽

White Light Endoscopy

Download Full-text

Integrated Multifunctional Laryngoscope for Medical Diagnosis and Treatment

Applied Sciences ◽

10.3390/app10217491 ◽

2020 ◽

Vol 10 (21) ◽

pp. 7491

Author(s):

Shanshan Liang ◽

Xinyu Li ◽

Jiajing Kang ◽

Jiebin Zou ◽

Faya Liang ◽

...

Keyword(s):

Laser Ablation ◽

Early Diagnosis ◽

White Light ◽

Ex Vivo ◽

Imaging Evaluation ◽

Cross Sectional ◽

White Light Endoscopy ◽

Common Path ◽

Path Design ◽

Laryngeal Tumors

Laryngeal lesions can cause great inconvenience to patients. Early diagnosis and corresponding treatments are critical to the survival of patients. However, the diagnosis and precise removal of tumors remain a challenge under the use of a white light laryngoscope. In this work, an integrated, multifunctional laryngoscope was designed and tested for the imaging evaluation and precision laser surgery for laryngeal tissue. This integrated diagnostic and therapeutic endoscopic system included two imaging modes (i.e., optical coherence tomography and white light endoscopy) and a laser ablation treatment mode. The endoscope had a common-path design to ensure that the same position could be imaged and treated simultaneously. The ex vivo porcine larynx experimental results showed that the system imaging modes could simultaneously acquire both superficial and cross-sectional images of the sample tissue, and the ablation treatment could be performed under imaging guidance. This multifunctional laryngoscope has great potential for the early diagnosis of and accurate laser ablation surgery for laryngeal tumors.

Download Full-text

Real-time in-vivo microscopic imaging of the cervix using confocal laser endomicroscopy

European Journal of Cancer Prevention ◽

10.1097/cej.0000000000000188 ◽

2016 ◽

Vol 25 (4) ◽

pp. 335-343 ◽

Cited By ~ 5

Author(s):

Michel Degueldre ◽

Jean Vandromme ◽

Alexander de Wind ◽

Francesco Feoli

Keyword(s):

Real Time ◽

Confocal Laser Endomicroscopy ◽

Confocal Laser ◽

Microscopic Imaging

Download Full-text

Real-Time Element Movement in a Plant

Novel Plant Imaging and Analysis ◽

10.1007/978-981-33-4992-6_4 ◽

2021 ◽

pp. 109-168

Author(s):

Tomoko M. Nakanishi

Keyword(s):

Real Time ◽

Imaging System ◽

Radioactive Cesium ◽

Soil Culture ◽

Basic Study ◽

Water Culture ◽

Microscopic Imaging ◽

Expression Site ◽

The Difference ◽

Element Movement

AbstractWe developed an imaging method utilizing the available RIs. We developed two types of real-time RI imaging systems (RRIS), one for macroscopic imaging and the other for microscopic imaging. The principle of visualization was the same, converting the radiation to light by a Cs(Tl)I scintillator deposited on a fiber optic plate (FOS). Many nuclides were employed, including 14C, 18F, 22Na, 28Mg, 32P 33P, 35S, 42K, 45Ca, 48V, 54Mn, 55Fe, 59Fe, 65Zn, 86Rb, 109Cd, and 137Cs.Since radiation can penetrate the soil as well as water, the difference between soil culture and water culture was visualized. 137Cs was hardly absorbed by rice roots growing in soil, whereas water culture showed high absorption, which could provide some reassurance after the Fukushima Nuclear Accident and could indicate an important role of soil in firmly adsorbing the radioactive cesium.28Mg and 42K, whose production methods were presented, were applied for RRIS to visualize the absorption image from the roots. In addition to 28Mg and 42K, many nuclides were applied to image absorption in the roots. Each element showed a specific absorption speed and accumulation pattern. The image analysis of the absorption of Mg is presented as an example. Through successive images of the element absorption, phloem flow in the aboveground part of the plant was analyzed. The element absorption was visualized not only in the roots but also in the leaves, a basic study of foliar fertilization.In the case of the microscopic imaging system, a fluorescence microscope was modified to acquire three images at the same time: a light image, fluorescent image, and radiation image. Although the resolution of the image was estimated to be approximately 50 μm, superposition showed the expression site of the transporter gene and the actual 32P-phosphate absorption site to be the same in Arabidopsis roots.

Download Full-text