scholarly journals Rational Design of ICT-Based Fluorescent Probe with AIE and NIR Properties for Hypochlorite Determination

2022 ◽  
Author(s):  
Chenggong Xu ◽  
tian wu ◽  
Lizheng Duan ◽  
Yunhao Zhou ◽  
Yanmei Zhou
Keyword(s):  
Fluorescent Probes ◽  
Live Cell Imaging ◽  
Rational Design ◽  
Near Infrared ◽  
Cell Imaging ◽  
Intramolecular Charge Transfer ◽  
Tap Water ◽  
Food Samples ◽  
Synthesis Process ◽  
Nir Fluorescence

Abstract In recent years, the easily synthesized fluorescent probes with good photophysical and sensing properties have attracted widespread attention. Herein, by utilizing the fluorescence regulation effect of electron push-pull effect and the oxidation property of hypochlorite (ClO-) to C=N double bonds, we proposed two intramolecular charge transfer (ICT)-based fluorescent probes with typical aggregation-induced emission (AIE) properties for ClO- detection. The synthesis process of the two probes is very convenient, and both of them can exhibit significant colorimetric and fluorescence changes within 3 min in the presence of ClO-. Moreover, compared with Probe A, the Probe B with near-infrared (NIR) fluorescence centered at 677 nm was successfully applied to ClO- determination in tap water and food samples as well as live cell imaging.

Rational design of reversible fluorescent probes for live-cell imaging and quantification of fast glutathione dynamics

2016 ◽  
Vol 9 (3) ◽  
pp. 279-286 ◽  
Author(s):  
Keitaro Umezawa ◽  
Masafumi Yoshida ◽  
Mako Kamiya ◽  
Tatsuya Yamasoba ◽  
Yasuteru Urano
Keyword(s):  
Fluorescent Probes ◽  
Live Cell Imaging ◽  
Rational Design ◽  
Cell Imaging ◽  
Live Cell

scholarly journals “Probe, Sample, and Instrument (PSI)”: The Hat-Trick for Fluorescence Live Cell Imaging

Chemosensors ◽  
2018 ◽  
Vol 6 (3) ◽  
pp. 40 ◽  
Author(s):  
Ludovic Galas ◽  
Thibault Gallavardin ◽  
Magalie Bénard ◽  
Arnaud Lehner ◽  
Damien Schapman ◽  
...  
Keyword(s):  
Organic Synthesis ◽  
Fluorescent Probes ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Cell Labeling ◽  
Key Points ◽  
Research Infrastructures ◽  
New Strategies ◽  
Fluorescence Live Cell Imaging

Cell Imaging Platforms (CIPs) are research infrastructures offering support to a number of scientific projects including the choice of adapted fluorescent probes for live cell imaging. What to detect in what type of sample and for how long is a major issue with fluorescent probes and, for this, the “hat-trick” “Probe–Sample–Instrument” (PSI) has to be considered. We propose here to deal with key points usually discussed in CIPs including the properties of fluorescent organic probes, the modality of cell labeling, and the best equipment to obtain appropriate spectral, spatial, and temporal resolution. New strategies in organic synthesis and click chemistry for accessing probes with enhanced photophysical characteristics and targeting abilities will also be addressed. Finally, methods for image processing will be described to optimize exploitation of fluorescence signals.

scholarly journals A Near‐Infrared Photoswitchable Protein–Fluorophore Tag for No‐Wash Live Cell Imaging

2018 ◽  
Vol 130 (49) ◽  
pp. 16315-16319 ◽  
Author(s):  
Wei Sheng ◽  
Setare Tahmasebi Nick ◽  
Elizabeth M. Santos ◽  
Xinliang Ding ◽  
Jun Zhang ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Near Infrared ◽  
Cell Imaging ◽  
Live Cell

scholarly journals Optimization of Advanced Live-Cell Imaging through Red/Near-Infrared Dye Labeling and Fluorescence Lifetime-Based Strategies

2021 ◽  
Vol 22 (20) ◽  
pp. 11092
Author(s):  
Magalie Bénard ◽  
Damien Schapman ◽  
Christophe Chamot ◽  
Fatéméh Dubois ◽  
Guénaëlle Levallet ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Live Cell Imaging ◽  
Near Infrared ◽  
Cell Imaging ◽  
Light Exposure ◽  
Photon Counting ◽  
Live Cell ◽  
Stimulated Emission ◽  
Plot Analysis ◽  
Phasor Plot

Fluorescence microscopy is essential for a detailed understanding of cellular processes; however, live-cell preservation during imaging is a matter of debate. In this study, we proposed a guide to optimize advanced light microscopy approaches by reducing light exposure through fluorescence lifetime (τ) exploitation of red/near-infrared dyes. Firstly, we characterized key instrumental elements which revealed that red/near-infrared laser lines with an 86x (Numerical Aperture (NA) = 1.2, water immersion) objective allowed high transmission of fluorescence signals, low irradiance and super-resolution. As a combination of two technologies, i.e., vacuum tubes (e.g., photomultiplier) and semiconductor microelectronics (e.g., avalanche photodiode), type S, X and R of hybrid detectors (HyD-S, HyD-X and HyD-R) were particularly adapted for red/near-infrared photon counting and τ separation. Secondly, we tested and compared lifetime-based imaging including coarse τ separation for confocal microscopy, fitting and phasor plot analysis for fluorescence lifetime microscopy (FLIM), and lifetimes weighting for enhanced stimulated emission depletion (STED) nanoscopy, in light of red/near-infrared multiplexing. Mainly, we showed that the choice of appropriate imaging approach may depend on fluorochrome number, together with their spectral/lifetime characteristics and STED compatibility. Photon-counting mode and sensitivity of HyDs together with phasor plot analysis of fluorescence lifetimes enabled the flexible and fast imaging of multi-labeled living H28 cells. Therefore, a combination of red/near-infrared dyes labeling with lifetime-based strategies offers new perspectives for live-cell imaging by enhancing sample preservation through acquisition time and light exposure reduction.

Near-Infrared-Emitting Iridium(III) Complexes as Phosphorescent Dyes for Live Cell Imaging

2013 ◽  
Vol 33 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Guoliang Zhang ◽  
Huiyuan Zhang ◽  
Yuan Gao ◽  
Ran Tao ◽  
Lijun Xin ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Near Infrared ◽  
Cell Imaging ◽  
Live Cell ◽  
Phosphorescent Dyes

scholarly journals A NIR-emitting cyanine with large Stokes shifts for live cell imaging: large impact of the phenol group on emission

2019 ◽  
Vol 55 (88) ◽  
pp. 13223-13226 ◽  
Author(s):  
Dipendra Dahal ◽  
Lucas McDonald ◽  
Sabita Pokhrel ◽  
Sailaja Paruchuri ◽  
Michael Konopka ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Near Infrared ◽  
Cell Imaging ◽  
Live Cell ◽  
Molecular Probe ◽  
Large Impact ◽  
Phenol Group ◽  
Stokes Shifts ◽  
Excellent Selectivity

A near-infrared (NIR) emitting (λem = 700–900 nm) molecular probe exhibits excellent selectivity towards mitochondria for live-cell imaging.

Clinical use of organic near-infrared fluorescent contrast agents in image-guided oncologic procedures and its potential in veterinary oncology

2018 ◽  
Vol 183 (11) ◽  
pp. 354-354 ◽  
Author(s):  
Sophie Favril ◽  
Eline Abma ◽  
Francesco Blasi ◽  
Emmelie Stock ◽  
Nausikaa Devriendt ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Fluorescent Probes ◽  
Near Infrared ◽  
Sentinel Lymph Node Mapping ◽  
Cost Effective ◽  
Surgical Oncology ◽  
Great Promise ◽  
Lymph Node Mapping ◽  
Nir Light ◽  
Nir Fluorescence

One of the major challenges in surgical oncology is the intraoperative discrimination of tumoural versus healthy tissue. Until today, surgeons rely on visual inspection and palpation to define the tumoural margins during surgery and, unfortunately, for various cancer types, the local recurrence rate thus remains unacceptably high. Near-infrared (NIR) fluorescence imaging is an optical imaging technique that can provide real-time preoperative and intraoperative information after administration of a fluorescent probe that emits NIR light once exposed to a NIR light source. This technique is safe, cost-effective and technically easy. Several NIR fluorescent probes are currently studied for their ability to highlight neoplastic cells. In addition, NIR fluorescence imaging holds great promise for sentinel lymph node mapping. The aim of this manuscript is to provide a literature review of the current organic NIR fluorescent probes tested in the light of human oncology and to introduce fluorescence imaging as a valuable asset in veterinary oncology.

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