Immunofluorescent localization of renin in mouse submaxillary gland and kidney.

1978 ◽  
Vol 234 (5) ◽  
pp. E480
Author(s):  
J W Menzie ◽  
L H Hoffman ◽  
A M Michelakis
Keyword(s):  
Direct Evidence ◽  
Submaxillary Gland ◽  
Juxtaglomerular Apparatus ◽  
Mouse Kidney ◽  
Male Mice ◽  
Immunofluorescent Localization ◽  
Submaxillary Glands ◽  
Mouse Submaxillary Gland

An antibody prepared against purified submaxillary renin was used to determine the site of renin concentration in male mice using immunofluorescent localization. The results provide direct evidence that the granular tubules of the submaxillary glands are the source of submaxillary renin. The antibody against submaxillary renin cross-reacts with kidney renin as evidenced by immunofluorescent localization in the juxtaglomerular apparatus of mouse kidney.

Renin Precursor from Mouse Kidney Identified by Cell-Free Translation of Messenger RNA

1980 ◽  
Vol 59 (4) ◽  
pp. 297-299 ◽  
Author(s):  
K. Poulsen ◽  
J. Vuust ◽  
T. Lund
Keyword(s):  
Molecular Weight ◽  
Messenger Rna ◽  
Submaxillary Gland ◽  
Mouse Kidney ◽  
Single Chain ◽  
Active Renin ◽  
Biosynthetic Precursor ◽  
Mouse Submaxillary Gland ◽  
Free Translation

1. The biosynthetic precursor of renin (pre-prorenin) from mouse kidney is a single chain polypeptide with a molecular weight of 50 000. 2. This is the same value as previously found for mouse submaxillary gland pre-prorenin. 3. Mouse kidney pre-prorenin (mol. wt. 50 000) is larger than the enzymatically active renin (mol. wt. 40 000).

scholarly journals Whole body autoradiographic distribution of exogenously administered renin in mice.

1983 ◽  
Vol 31 (6) ◽  
pp. 776-782 ◽  
Author(s):  
H Iwao ◽  
N Nakamura ◽  
F Ikemoto ◽  
K Yamamoto
Keyword(s):  
Radioactive Iodine ◽  
Adrenal Glands ◽  
Renal Cortex ◽  
Submaxillary Gland ◽  
Intestinal Content ◽  
Whole Body ◽  
Submaxillary Glands ◽  
Mouse Submaxillary Gland ◽  
Thyroid Glands ◽  
The Brain

The distribution of exogenously administered renin was investigated using whole body autoradiography. Purified renin from mouse submaxillary gland (SR) was labeled with radioactive iodine (125I). This labeled renin (125I-SR) and Na125I were administered into the tail vein of male ddY mice, in doses of 10.2 and 16.4 mu Ci/30 g body weight, respectively. Mice were killed by an overdose of ether, and autoradiography was performed on whole body sections. To separate free 125I liberated from 125I-SR, sections were treated with perchloric acid. A major accumulation of 125I-SR, acid-insoluble, was evident in the renal cortex, whereas the hepatic accumulation of 125I-SR was minor. Radioactivity in the thyroid and submaxillary glands, in the stomach, and in urine was also apparent, but disappeared after acid treatment, except in the thyroid glands. Radioactivity in the brain, intestinal content, spleen, and adrenal glands was nil. These autoradiograms provide the first evidence that exogenously administered renin is mainly distributed in the renal cortex.

On the in Vitro Behaviour of Mouse Submaxillary Gland Cells

1973 ◽  
Vol 13 (2) ◽  
pp. 441-445
Author(s):  
MARIA LUISA MARCANTE
Keyword(s):  
Endoplasmic Reticulum ◽  
Lactate Dehydrogenase ◽  
Submaxillary Gland ◽  
Biochemical Properties ◽  
Dark Cells ◽  
Submaxillary Glands ◽  
Mouse Submaxillary Gland ◽  
Good Agreement

Investigations on the morphological features and some biochemical properties of cells isolated from mouse C3H/He (IRE/Ar) submaxillary glands cultivated in vitro are reported. Two types of cells have been recognized: the dark cells differ from the light in the shape of their mitochondria, and number of vacuoles, while endoplasmic reticulum is absent. The enzymic activities, which have been tested, are phosphofructokinase, pyruvate kinase, amylase and lactate dehydrogenase. The values for enzyme activities in vitro are in good agreement with those obtained for submaxillary gland in vivo. Therefore, it can be concluded that during their growth in vitro cells from submaxillary glands retain their enzymic properties.

scholarly journals Preliminary X-ray crystallographic data on mouse submaxillary gland renin and renin-inhibitor complexes.

1984 ◽  
Vol 259 (20) ◽  
pp. 12714-12717
Author(s):  
M A Navia ◽  
J P Springer ◽  
M Poe ◽  
J Boger ◽  
K Hoogsteen
Keyword(s):  
Submaxillary Gland ◽  
Crystallographic Data ◽  
Renin Inhibitor ◽  
X Ray ◽  
Mouse Submaxillary Gland

Mesenchymal control over elongating and branching morphogenesis in salivary gland development

Development ◽  
1981 ◽  
Vol 66 (1) ◽  
pp. 209-221
Author(s):  
Hiroyuki Nogawa ◽  
Takeo Mizuno
Keyword(s):  
Salivary Gland ◽  
Salivary Glands ◽  
Submaxillary Gland ◽  
Branching Morphogenesis ◽  
Mouse Submaxillary Gland ◽  
Gland Development

Recombination of the epithelium and mesenchyme between quail anterior submaxillary gland (elongating type) and quail anterior lingual or mouse submaxillary gland (branching type) was effected in vitro to clarify whether the elongating morphogenesis was directed by the epithelial or the mesenchymal component. Quail anterior submaxillary epithelium recombined with quail anterior lingual or mouse submaxillary mesenchyme came to branch. Conversely, quail anterior lingual or 12-day mouse submaxillary epithelium recombined with quail anterior submaxillary mesenchyme came to elongate, though the mesenchyme was less effective with 13-day mouse submaxillary epithelium. These results suggest that the elongating or branching morphogenesis of quail salivary glands is controlled by the mesenchyme.

scholarly journals FURTHER STUDIES CONCERNING THE FILTRABLE VIRUS PRESENT IN THE SUBMAXILLARY GLANDS OF GUINEA PIGS

1927 ◽  
Vol 46 (6) ◽  
pp. 935-956 ◽  
Author(s):  
Ann G. Kuttner
Keyword(s):  
Guinea Pig ◽  
Guinea Pigs ◽  
Active Material ◽  
Active Agent ◽  
Submaxillary Gland ◽  
Intracerebral Inoculation ◽  
Submaxillary Glands ◽  
Direct Inoculation ◽  
In Series ◽  
Subcutaneous Inoculation

1. It has been shown that the guinea pig virus localizes in the submaxillary glands of young guinea pigs following subcutaneous, intraperitoneal, or intravenous injection of active material, and that the specific lesion is demonstrable in the glands in 12 to 15 days. When an active infection of the gland has been produced in this way, the guinea pigs are refractory to intracerebral inoculation of the virus. 2. No lesion develops in the submaxillary glands of young guinea pigs injected subcutaneously with guinea pig virus which has been inactivated by heat. Young guinea pigs which have received injections of heat-killed virus do not become refractory to intracerebral inoculation of the virus. 3. When young guinea pigs from which both submaxillary glands have been removed are injected subcutaneously with active virus, the virus localizes in the parotid gland, and the animals become refractory to intracerebral inoculation. 4. It has been impossible to demonstrate virucidal properties in the sera of adult guinea pigs which have become spontaneously infected with the virus, or in the sera of young guinea pigs which have been artificially rendered refractory to intracerebral inoculation. 5. It has been possible to transmit the virus from guinea pig to guinea pig continuously in series through seven animals by direct inoculation from submaxillary gland to submaxillary gland. 6. The fact that the virus regularly localizes in the submaxillary glands following subcutaneous inoculation has been utilized in passing the virus from guinea pig to guinea pig. 2 weeks after the subcutaneous inoculation of the virus into young guinea pigs, the active agent was present in the submaxillary glands. Emulsions of the submaxillary glands of these animals were then used for the subcutaneous injection of another group of young guinea pigs. In this way the virus was transmitted continuously from skin to submaxillary gland through a series of seven animals.

Rapid and large-scale purification and characterization of renin from mouse submaxillary gland

1982 ◽  
Vol 217 (2) ◽  
pp. 574-581 ◽  
Author(s):  
Kunio S. Misono ◽  
Leslie A. Holladay ◽  
Kazuo Murakami ◽  
Kenji Kuromizu ◽  
Tadashi Inagami
Keyword(s):  
Large Scale ◽  
Submaxillary Gland ◽  
Purification And Characterization ◽  
Mouse Submaxillary Gland
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