Downregulation of the renin-angiotensin system in streptozotocin-diabetic rats

1992 ◽  
Vol 262 (1) ◽  
pp. E105-E109 ◽  
Author(s):  
L. A. Cassis
Keyword(s):  
Adipose Tissue ◽  
Replacement Therapy ◽  
White Adipose Tissue ◽  
Plasma Concentrations ◽  
Renin Angiotensin System ◽  
Diabetic Rats ◽  
Angiotensin System ◽  
Renin Angiotensin ◽  
Brown Adipose ◽  
Insulin Replacement

To determine if insulin has the ability to regulate components of the renin-angiotensin system, renin and angiotensinogen mRNA and plasma concentrations were determined in 4-wk streptozotocin (STZ)-diabetic rats. In another group of STZ-diabetic rats, replacement insulin therapy was given over the 4-wk period, and the above parameters were examined. In STZ-diabetic rats, there was a significant regression of white adipose tissue that was accompanied by an increase in the yield of RNA obtained. Changes in white adipose tissue were reversed by insulin replacement therapy in STZ-diabetic rats. There were no changes in brown adipose tissue weight or RNA yield in STZ-diabetic rats. Plasma renin activity (PRA) was significantly decreased in STZ-diabetic rats; however, plasma angiotensinogen concentration was not significantly affected by diabetes. PRA was restored to control levels in STZ-diabetic rats with insulin replacement. Kidney renin mRNA as well as liver, epididymal, and interscapular fat angiotensinogen mRNA were significantly decreased in STZ-diabetic rats. Renin and angiotensinogen mRNA were not significantly different from control in all tissues examined in STZ-diabetic rats with insulin replacement therapy. Results from this study suggest a downregulation of the renin-angiotensin system in 4-wk STZ-diabetic rats at the level of mRNA expression that is restored by replacement therapy with insulin; therefore, insulin may directly or indirectly regulate the renin-angiotensin system.

scholarly journals Comparative expression analysis of the renin–angiotensin system components between white and brown perivascular adipose tissue

2008 ◽  
Vol 197 (1) ◽  
pp. 55-64 ◽  
Author(s):  
B Gálvez-Prieto ◽  
J Bolbrinker ◽  
P Stucchi ◽  
A I de las Heras ◽  
B Merino ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Renin Angiotensin System ◽  
Receptor Expression ◽  
Mrna Levels ◽  
Ang Ii ◽  
Perivascular Adipose Tissue ◽  
Angiotensin System ◽  
Renin Angiotensin ◽  
Vascular Bed ◽  
Brown Adipose

Recent studies have demonstrated that the rat adipose tissue expresses some of the components necessary for the production of angiotensin II (Ang II) and the receptors mediating its actions. The aim of this work is to characterize the expression of the renin–angiotensin system (RAS) components in perivascular adipose tissue and to assess differences in the expression pattern depending on the vascular bed and type of adipose tissue. We analyzed Ang I and Ang II levels as well as mRNA levels of RAS components by a quantitative RT-PCR method in periaortic (PAT) and mesenteric adipose tissue (MAT) of 3-month-old male Wistar–Kyoto rats. PAT was identified as brown adipose tissue expressing uncoupling protein-1 (UCP-1). It had smaller adipocytes than those from MAT, which was identified as white adipose tissue. All RAS components, except renin, were detected in both PAT and MAT. Levels of expression of angiotensinogen, Ang-converting enzyme (ACE), and ACE2 were similar between PAT and MAT. Renin receptor expression was five times higher, whereas expression of chymase, AT1a, and AT2 receptors were significantly lower in PAT compared with MAT respectively. In addition, three isoforms of the AT1a receptor were found in perivascular adipose tissue. The AT1b receptor was found at very a low expression level. Ang II levels were higher in MAT with no differences between tissues in Ang I. The results show that the RAS is differentially expressed in white and brown perivascular adipose tissues implicating a different role for the system depending on the vascular bed and the type of adipose tissue.

scholarly journals Açaí seed extract prevents the renin-angiotensin system activation, oxidative stress and inflammation in white adipose tissue of high-fat diet–fed mice

2020 ◽  
Vol 79 ◽  
pp. 35-49 ◽  
Author(s):  
Izabelle Barcellos Santos ◽  
Graziele Freitas de Bem ◽  
Cristiane Aguiar da Costa ◽  
Lenize Costa Reis Marins de Carvalho ◽  
Amanda Faria de Medeiros ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Adipose Tissue ◽  
White Adipose Tissue ◽  
High Fat Diet ◽  
Renin Angiotensin System ◽  
Seed Extract ◽  
High Fat ◽  
Angiotensin System ◽  
Renin Angiotensin ◽  
System Activation

scholarly journals Regulation and Functions of the Renin-Angiotensin System in White and Brown Adipose Tissue

2017 ◽  
pp. 1137-1150 ◽  
Author(s):  
Mandana Pahlavani ◽  
Nishan. S Kalupahana ◽  
Latha Ramalingam ◽  
Naima Moustaid-Moussa
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Renin Angiotensin System ◽  
Angiotensin System ◽  
Renin Angiotensin ◽  
Brown Adipose

Age-Related Changes of Renin-Angiotensin System Genes in White Adipose Tissue of Rats

2002 ◽  
Vol 34 (11/12) ◽  
pp. 716-720 ◽  
Author(s):  
F. Adams ◽  
P. Wiedmer ◽  
K. Gorzelniak ◽  
S. Engeli ◽  
S. Klaus ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
White Adipose Tissue ◽  
Renin Angiotensin System ◽  
Angiotensin System ◽  
Renin Angiotensin ◽  
Age Related ◽  
Age Related Changes

Activation of the systemic and adipose renin-angiotensin system in rats with diet-induced obesity and hypertension

2004 ◽  
Vol 287 (4) ◽  
pp. R943-R949 ◽  
Author(s):  
Carine M. Boustany ◽  
Kalyani Bharadwaj ◽  
Alan Daugherty ◽  
David R. Brown ◽  
David C. Randall ◽  
...  
Keyword(s):  
Body Weight ◽  
Adipose Tissue ◽  
Body Weight Gain ◽  
Plasma Concentrations ◽  
Renin Angiotensin System ◽  
Sprague Dawley ◽  
High Fat ◽  
Angiotensin System ◽  
Renin Angiotensin ◽  
Arterial Blood

In obesity-related hypertension, activation of the renin-angiotensin system (RAS) has been reported despite marked fluid volume expansion. Adipose tissue expresses components of the RAS and is markedly expanded in obesity. This study evaluated changes in components of the adipose and systemic RAS in diet-induced obese hypertensive rats. RAS was quantified in adipose tissue and compared with primary sources for the circulating RAS. Male Sprague-Dawley rats were fed either a low-fat (LF; 11% kcal as fat) or moderately high-fat (32% kcal as fat) diet for 11 wk. After 8 wk, rats fed the moderately high-fat diet segregated into obesity-prone (OP) and obesity-resistant (OR) groups based on their body weight gain (body weight: OR, 566 ± 10; OP, 702 ± 20 g; P < 0.05). Mean arterial blood pressure was increased in OP rats (LF: 97 ± 2; OR: 97 ± 2; OP: 105 ± 1 mmHg; P < 0.05). Quantification of mRNA expression by real-time PCR demonstrated a selective increase (2-fold) in angiotensinogen gene expression in retroperitoneal adipose tissue from OP vs. OR and LF rats. Similarly, plasma angiotensinogen concentration was increased in OP rats (LF: 390 ± 48; OR: 355 ± 24; OP: 530 ± 22 ng/ml; P < 0.05). In contrast, other components of the RAS were not altered in OP rats. Marked increases in the plasma concentrations of angiotensin peptides were observed in OP rats (angiotensin II: LF: 95 ± 31; OR: 59 ± 20; OP: 295 ± 118 pg/ml; P < 0.05). These results demonstrate increased activity of the adipose and systemic RAS in obesity-related hypertension.

scholarly journals Impact of Exercise Training and Enalapril, either alone or in Combination, on White Adipose Tissue Renin‐Angiotensin System in a Diet‐Induced Obesity Model

2018 ◽  
Vol 32 (S1) ◽  
Author(s):  
Agatha Cristie Menezes ◽  
Isabele Gomes Giori ◽  
Beatriz Alexandre ◽  
Mariana Sodré Andrade ◽  
Fernanda Marques Freitas ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Exercise Training ◽  
White Adipose Tissue ◽  
Renin Angiotensin System ◽  
Angiotensin System ◽  
Renin Angiotensin ◽  
Diet Induced Obesity
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