Angiotensin II and tumor necrosis factor-α synergistically promote monocyte chemoattractant protein-1 expression: roles of NF-κB, p38, and reactive oxygen species
We examined whether ANG II and TNF-α cooperatively induce vascular inflammation using the expression of monocyte chemoattractant protein (MCP)-1 as a marker of vascular inflammation. ANG II and TNF-α stimulated MCP-1 expression in a synergistic manner in vascular smooth muscle cells. ANG II-induced MCP-1 expression was potently inhibited to a nonstimulated basal level by blockade of the p38-dependent pathway but only partially inhibited by blockade of the NF-κB-dependent pathway. In contrast, TNF-α-induced MCP-1 expression was potently suppressed by blockade of NF-κB activation but only modestly suppressed by blockade of p38 activation. ANG II- and TNF-α-induced activation of NF-κB- and p38-dependent pathways was partially inhibited by pharmacological inhibitors of ROS production. Furthermore, ANG II- and TNF-α-stimulated MCP-1 expression was partially suppressed by ROS inhibitors. We also examined whether endogenous ANG II and TNF-α cooperatively promote vascular inflammation in vivo using a wire injury model of the rat femoral artery. Blockade of both ANG II and TNF-α further suppressed neointimal formation, macrophage infiltration, and MCP-1 expression in an additive manner compared with blockade of ANG II or TNF-α alone. These results suggested that ANG II and TNF-α synergistically stimulate MCP-1 expression via the utilization of distinct intracellular signaling pathways (p38- and NFκB-dependent pathways) and that these pathways are activated in ROS-dependent and -independent manners. These results also suggest that ANG II and TNF-α cooperatively stimulate vascular inflammation in vivo as well as in vitro.