Early effects of ageing on the mechanical performance of isolated locomotory (EDL) and respiratory (diaphragm) skeletal muscle using the work-loop technique

2014 ◽  
Vol 307 (6) ◽  
pp. R670-R684 ◽  
Author(s):  
Jason Tallis ◽  
Rob S. James ◽  
Alexander G. Little ◽  
Val M. Cox ◽  
Michael J. Duncan ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Power Output ◽  
Muscle Performance ◽  
Maximal Power ◽  
Maximal Power Output ◽  
Age Related ◽  
Loop Technique ◽  
Edl Muscle ◽  
Work Loop

Previous isolated muscle studies examining the effects of ageing on contractility have used isometric protocols, which have been shown to have poor relevance to dynamic muscle performance in vivo. The present study uniquely uses the work-loop technique for a more realistic estimation of in vivo muscle function to examine changes in mammalian skeletal muscle mechanical properties with age. Measurements of maximal isometric stress, activation and relaxation time, maximal power output, and sustained power output during repetitive activation and recovery are compared in locomotory extensor digitorum longus (EDL) and core diaphragm muscle isolated from 3-, 10-, 30-, and 50-wk-old female mice to examine the early onset of ageing. A progressive age-related reduction in maximal isometric stress that was of greater magnitude than the decrease in maximal power output occurred in both muscles. Maximal force and power developed earlier in diaphragm than EDL muscle but demonstrated a greater age-related decline. The present study indicates that ability to sustain skeletal muscle power output through repetitive contraction is age- and muscle-dependent, which may help rationalize previously reported equivocal results from examination of the effect of age on muscular endurance. The age-related decline in EDL muscle performance is prevalent without a significant reduction in muscle mass, and biochemical analysis of key marker enzymes suggests that although there is some evidence of a more oxidative fiber type, this is not the primary contributor to the early age-related reduction in muscle contractility.

scholarly journals Frequency of Maximal Power Output at in vivo Myofilament Lattice Spacing Matches Drosophila Wing Beat Frequency

2011 ◽  
Vol 100 (3) ◽  
pp. 12a
Author(s):  
Bertrand C.W. Tanner ◽  
Gerrie P. Farman ◽  
Thomas C. Irving ◽  
David W. Maughan ◽  
Mark S. Miller
Keyword(s):  
Power Output ◽  
Lattice Spacing ◽  
Beat Frequency ◽  
Wing Beat ◽  
Maximal Power ◽  
Maximal Power Output ◽  
Wing Beat Frequency ◽  
Drosophila Wing

CONTRACTILE PROPERTIES OF A HIGH-FREQUENCY MUSCLE FROM A CRUSTACEAN - MECHANICAL POWER OUTPUT

1994 ◽  
Vol 187 (1) ◽  
pp. 295-303 ◽  
Author(s):  
R Josephson ◽  
D Stokes
Keyword(s):  
Power Output ◽  
High Frequency ◽  
Carcinus Maenas ◽  
Mechanical Power ◽  
Abductor Muscle ◽  
Mechanical Power Output ◽  
Loop Technique ◽  
Number Of Cycles ◽  
Work Loop

The mechanical power output during oscillatory contraction was determined for the flagellum abductor muscle of the crab Carcinus maenas using the work loop technique. Measurements were made at 10 Hz, which is the normal operating frequency of the muscle. The temperature was 15 °C. Increasing the number of stimuli per cycle (given at an interstimulus interval of 3.3 ms) decreased the number of cycles required to reach a work plateau and increased the work per cycle at the plateau to a maximum at 4­5 stimuli per cycle. The maximum mechanical power output was 9.7 W kg-1 muscle (about 26 W kg-1 myofibril). The optimum strain for work output (5.7 %) was close to the estimated muscle strain in vivo (5.2 %).

Skeletal muscle structural and energetic characteristics in subjects with sickle cell trait, α-thalassemia, or dual hemoglobinopathy

2010 ◽  
Vol 109 (3) ◽  
pp. 728-734 ◽  
Author(s):  
Lucile Vincent ◽  
Léonard Féasson ◽  
Samuel Oyono-Enguéllé ◽  
Viviane Banimbek ◽  
Géraldine Monchanin ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Sickle Cell ◽  
Power Output ◽  
Citrate Synthase ◽  
Sickle Cell Trait ◽  
Fiber Type ◽  
Time To Exhaustion ◽  
Fiber Types ◽  
Maximal Power ◽  
Maximal Power Output

Previous studies have shown that subjects with sickle cell trait (SCT), α-thalassemia (α-t), and the dual hemoglobinopathy (SCT/α-t) manifest subtle, albeit significant, differences during exercise. To better understand such differences, we assessed skeletal muscle histomorphological and energetic characteristics in 10 control HbAA subjects (C), 5 subjects with α-t (α-t), 6 SCT carriers (SCT) and 9 SCT carriers with α-t (SCT/α-t). Subjects underwent a muscle biopsy and also performed an incremental maximal exercise and a time to exhaustion test. There were no observable differences in daily energy expenditure, maximal power output (Pmax), or time to exhaustion at 110% Pmax ( Tex) among the groups. Blood lactate concentrations measured at the end of the Tex, muscle fiber type distribution, and mean phosphofructokinase (PFK), lactate dehydrogenase (LDH), β-hydroxyacyl-CoA-dehydrogenase (HAD), and citrate synthase (CS) activities were all similar among the four groups. However, SCT was associated with a lower cytochrome- c oxidase (COx) activity in type IIa fibers ( P < 0.05), and similar trends were observed in fiber types I and IIx. Trends toward lower creatine kinase (CK) activity ( P = 0.0702) and higher surface area of type IIx fibers were observed in SCT ( P = 0.0925). In summary, these findings support most of the previous observations in SCT, such as 1) similar maximal power output and associated maximal oxygen consumption (V̇o2max) values and 2) lower exercise performances during prolonged submaximal exercise. Furthermore, performances during short supramaximal exercise were not different in SCT. Finally, the dual hemoglobinopathy condition does not seem to affect muscle characteristics.

scholarly journals Skeletal muscle ATP turnover and muscle fiber conduction velocity are elevated at higher muscle temperatures during maximal power output development in humans

2006 ◽  
Vol 290 (2) ◽  
pp. R376-R382 ◽  
Author(s):  
Stuart R. Gray ◽  
Giuseppe De Vito ◽  
Myra A. Nimmo ◽  
Dario Farina ◽  
Richard A. Ferguson
Keyword(s):  
Skeletal Muscle ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Power Output ◽  
Effect Of Temperature ◽  
Muscle Temperature ◽  
Maximal Power ◽  
Maximal Power Output ◽  
Muscle Fiber Conduction Velocity ◽  
Atp Turnover

The effect of temperature on skeletal muscle ATP turnover and muscle fiber conduction velocity (MFCV) was studied during maximal power output development in humans. Eight male subjects performed a 6-s maximal sprint on a mechanically braked cycle ergometer under conditions of normal (N) and elevated muscle temperature (ET). Muscle temperature was passively elevated through the combination of hot water immersion and electric blankets. Anaerobic ATP turnover was calculated from analysis of muscle biopsies obtained before and immediately after exercise. MFCV was measured during exercise using surface electromyography. Preexercise muscle temperature was 34.2°C (SD 0.6) in N and 37.5°C (SD 0.6) in ET. During ET, the rate of ATP turnover for phosphocreatine utilization [temperature coefficient (Q10) = 3.8], glycolysis (Q10 = 1.7), and total anaerobic ATP turnover [Q10 = 2.7; 10.8 (SD 1.9) vs. 14.6 mmol·kg−1 (dry mass)·s−1 (SD 2.3)] were greater than during N ( P < 0.05). MFCV was also greater in ET than in N [3.79 (SD 0.47) to 5.55 m/s (SD 0.72)]. Maximal power output (Q10 = 2.2) and pedal rate (Q10 = 1.6) were greater in ET compared with N ( P < 0.05). The Q10 of maximal and mean power were correlated ( P < 0.05; R = 0.82 and 0.85, respectively) with the percentage of myosin heavy chain type IIA. The greater power output obtained with passive heating was achieved through an elevated rate of anaerobic ATP turnover and MFCV, possibly due to a greater effect of temperature on power production of fibers, with a predominance of myosin heavy chain IIA at the contraction frequencies reached.

Molecular adaptations in human skeletal muscle to endurance training under simulated hypoxic conditions

2001 ◽  
Vol 91 (1) ◽  
pp. 173-182 ◽  
Author(s):  
M. Vogt ◽  
A. Puntschart ◽  
J. Geiser ◽  
C. Zuleger ◽  
R. Billeter ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Power Output ◽  
High Intensity ◽  
Human Skeletal Muscle ◽  
Fine Tuning ◽  
Maximal Power ◽  
Maximal Power Output ◽  
High Intensity Training ◽  
Hypoxic Conditions ◽  
Intensity Training

This study was performed to explore changes in gene expression as a consequence of exercise training at two levels of intensity under normoxic and normobaric hypoxic conditions (corresponding to an altitude of 3,850 m). Four groups of human subjects trained five times a week for a total of 6 wk on a bicycle ergometer. Muscle biopsies were taken, and performance tests were carried out before and after the training period. Similar increases in maximal O2 uptake (8.3–13.1%) and maximal power output (11.4–20.8%) were found in all groups. RT-PCR revealed elevated mRNA concentrations of the α-subunit of hypoxia-inducible factor 1 (HIF-1) after both high- (+82.4%) and low (+78.4%)-intensity training under hypoxic conditions. The mRNA of HIF-1α736, a splice variant of HIF-1α newly detected in human skeletal muscle, was shown to be changed in a similar pattern as HIF-1α. Increased mRNA contents of myoglobin (+72.2%) and vascular endothelial growth factor (+52.4%) were evoked only after high-intensity training in hypoxia. Augmented mRNA levels of oxidative enzymes, phosphofructokinase, and heat shock protein 70 were found after high-intensity training under both hypoxic and normoxic conditions. Our findings suggest that HIF-1 is specifically involved in the regulation of muscle adaptations after hypoxia training. Fine-tuning of the training response is recognized at the molecular level, and with less sensitivity also at the structural level, but not at global functional responses like maximal O2 uptake or maximal power output.

scholarly journals The effect of physiological concentrations of caffeine on the power output of maximally and submaximally stimulated mouse EDL (fast) and soleus (slow) muscle

2012 ◽  
Vol 112 (1) ◽  
pp. 64-71 ◽  
Author(s):  
Jason Tallis ◽  
Rob S. James ◽  
Val M. Cox ◽  
Michael J. Duncan
Keyword(s):  
Power Output ◽  
Muscle Power ◽  
Fiber Type ◽  
Slow Muscle ◽  
Anaerobic Exercise ◽  
Significant Difference ◽  
Ergogenic Effects ◽  
Loop Technique ◽  
Edl Muscle ◽  
Work Loop

The ergogenic effects of caffeine in human exercise have been shown to improve endurance and anaerobic exercise performance. Previous work has demonstrated that 70 μM caffeine (physiological maximum) can directly increase mouse extensor digitorum longus (EDL) muscle power output (PO) in sprintlike activity by 3%. Our study used the work loop technique on isolated mouse muscles to investigate whether the direct effect of 70 μM caffeine on PO differed between 1) maximally and submaximally activated muscle; 2) relatively fast (EDL) and relatively slow (soleus) muscles; and 3) caffeine concentrations. Caffeine treatment of 70 μM resulted in significant improvements in PO in maximally and submaximally activated EDL and soleus ( P < 0.03 in all cases). For EDL, the effects of caffeine were greatest when the lowest, submaximal stimulation frequency was used ( P < 0.001). Caffeine treatments of 140, 70, and 50 μM resulted in significant improvements in acute PO for both maximally activated EDL (3%) and soleus (6%) ( P < 0.023 in all cases); however, there was no significant difference in effect between these concentrations ( P > 0.420 in all cases). Therefore, the ergogenic effects of caffeine on PO were higher in muscles with a slower fiber type ( P < 0.001). Treatment with 35 μM caffeine failed to elicit any improvement in PO in either muscle ( P > 0.72 in both cases). Caffeine concentrations below the physiological maximum can directly potentiate skeletal muscle PO. This caffeine-induced increase in force could provide similar benefit across a range of exercise intensities, with greater gains likely in activities powered by slower muscle fiber type.

Power output and fatigue of human muscle in maximal cycling exercise

1983 ◽  
Vol 55 (1) ◽  
pp. 218-224 ◽  
Author(s):  
N. McCartney ◽  
G. J. Heigenhauser ◽  
N. L. Jones
Keyword(s):  
Power Output ◽  
Fiber Type ◽  
Average Power ◽  
Lactate Concentration ◽  
Peak Torque ◽  
Muscle Volume ◽  
Thigh Muscle ◽  
Peak Power Output ◽  
Maximal Power ◽  
Maximal Power Output

We studied maximal torque-velocity relationships and fatigue during short-term maximal exercise on a constant velocity cycle ergometer in 13 healthy male subjects. Maximum torque showed an inverse linear relationship to crank velocity between 60 and 160 rpm, and a direct relationship to thigh muscle volume measured by computerized tomography. Peak torque per liter thigh muscle volume (PT, N X ml-1) was related to crank velocity (CV, rpm) in the following equation: PT = 61.7 - 0.234 CV (r = 0.99). Peak power output was a parabolic function of crank velocity in individual subjects, but maximal power output was achieved at varying crank velocities in different subjects. Fiber type distribution was measured in the two subjects showing the greatest differences and demonstrated that a high proportion of type II fibers may be one factor associated with a high crank velocity for maximal power output. The decline in average power during 30 s of maximal effort was least at 60 rpm (23.7 +/- 4.6% of initial maximal power) and greatest at 140 rpm (58.7 +/- 6.5%). At 60 rpm the decline in power over 30 s was inversely related to maximal oxygen uptake (ml X min-1 X kg-1) (r = 0.69). Total work performed and plasma lactate concentration 3 min after completion of 30-s maximum effort were similar for each crank velocity.

scholarly journals Aerobic efficiency is associated with the improvement in maximal power output during acute hyperoxia

2017 ◽  
Vol 5 (2) ◽  
pp. e13119 ◽  
Author(s):  
Tom A. Manselin ◽  
Olof Södergård ◽  
Filip J. Larsen ◽  
Peter Lindholm
Keyword(s):  
Power Output ◽  
Maximal Power ◽  
Maximal Power Output

Effect of inspired O2 concentration on leg lactate release during incremental exercise

1996 ◽  
Vol 81 (1) ◽  
pp. 246-251 ◽  
Author(s):  
D. R. Knight ◽  
D. C. Poole ◽  
M. C. Hogan ◽  
D. E. Bebout ◽  
P. D. Wagner
Keyword(s):  
Blood Lactate ◽  
Power Output ◽  
Incremental Exercise ◽  
Venous Blood Flow ◽  
Maximal Power ◽  
Maximal Power Output ◽  
Lactate Accumulation ◽  
Lactate Release ◽  
O2 Concentration ◽  
Blood Lactate Accumulation

The normal rate of blood lactate accumulation during exercise is increased by hypoxia and decreased by hyperoxia. It is not known whether these changes are primarily determined by the lactate release in locomotory muscles or other tissues. Eleven men performed cycle exercise at 20, 35, 50, 92, and 100% of maximal power output while breathing 12, 21, and 100% O2. Leg lactate release was calculated at each stage of exercise as the product of femoral venous blood flow (thermodilution method) and femoral arteriovenous difference in blood lactate concentrations. Regression analysis showed that leg lactate release accounted for 90% of the variability in mean arterial lactate concentration at 20-92% maximal power output. This relationship was described by a regression line with a slope of 0.28 +/- 0.02 min/l and a y-intercept of 1.06 +/- 0.38 mmol/l (r2 = 0.90). There was no effect of inspired O2 concentration on this relationship (P > 0.05). We conclude that during continuous incremental exercise to fatigue the effect of inspired O2 concentration on blood lactate accumulation is principally determined by the rate of net lactate release in blood vessels of the locomotory muscles.

scholarly journals Age-related changes in collagen synthesis and degradation in rat tissues. Importance of degradation of newly synthesized collagen in regulating collagen production

1991 ◽  
Vol 276 (2) ◽  
pp. 307-313 ◽  
Author(s):  
P K Mays ◽  
R J McAnulty ◽  
J S Campa ◽  
G J Laurent
Keyword(s):  
Skeletal Muscle ◽  
Collagen Synthesis ◽  
Rat Tissues ◽  
Age Related ◽  
Developmental Growth ◽  
Cross Links ◽  
Fractional Synthesis ◽  
Synthesis And Degradation ◽  
Age Related Changes

During developmental growth, collagens are believed to be continuously deposited into an extracellular matrix which is increasingly stabilized by the formation of covalent cross-links throughout life. However, the age-related changes in rates of synthetic and degradative processes are less well understood. In the present study we measured rates of collagen synthesis in vivo using a flooding dose of unlabelled proline given with [14C]proline and determining production of hydroxy[14C]proline. Degradation of newly synthesized collagen was estimated from the amount of free hydroxy [14C]proline in tissues 30 min after injection. Collagen fractional synthesis rates ranged from about 5%/day in skeletal muscle to 20%/day in hearts of rats aged 1 month. At 15 months of age, collagen fractional synthesis rates had decreased markedly in lung and skin, but in skeletal muscle and heart, rates were unchanged. At 24 months of age, synthesis rates had decreased by at least 10-fold in all tissues, compared with rates at 1 month. The proportion of newly synthesized collagen degraded ranged from 6.4 +/- 0.4% in skin to 61.6 +/- 5.0% in heart at 1 month of age. During aging the proportion degraded increased in all tissues to maximal values at 15 months, ranging from 56 +/- 7% in skin to 96 +/- 1% in heart. These data suggest that there are marked age-related changes in rates of collagen metabolism. They also indicate that synthesis is active even in old animals, where the bulk of collagens produced are destined to be degraded.

Sign in / Sign up

Export Citation Format

Share Document