Reorganization of the innervation of the vas deferens after sympathetic decentralization

1996 ◽  
Vol 271 (6) ◽  
pp. R1481-R1488
Author(s):  
K. Kihara ◽  
H. Kakizaki ◽  
W. C. de Groat
Keyword(s):  
Electrical Stimulation ◽  
Vas Deferens ◽  
Ganglion Cells ◽  
Rat Vas Deferens ◽  
Pelvic Nerve ◽  
Hypogastric Nerve ◽  
Normal Side ◽  
Efferent Pathways ◽  
Stimulation Of

Reorganization of autonomic efferent pathways to the rat vas deferens was noted after chronic (30 days) sympathetic decentralization produced by hypogastric nerve (HGN) transection. In normal rats, electrical stimulation of the HGN elicited an increase in vasal pressure (VP) bilaterally, whereas pelvic nerve (PN) stimulation did not alter VP. However, after unilateral HGN transection, stimulation of the PN on the transected side but not on the normal side increased VP. The decentralized vas exhibited larger VP responses to stimulation of the contralateral HGN in comparison with the normal vas. After bilateral HGN transection, PN-induced VP responses were elicited at lower stimulus intensities than in rats with unilateral transections. PN-induced VP responses were blocked by hexamethonium and prazosin but were not altered by atropine. Distension of the vas lumen occurred after decentralization. PN-induced VP responses were not detectable in extremely distended vas. These data indicate that, after degeneration of sympathetic preganglionic axons, decentralized adrenergic ganglion cells are reinnervated by parasympathetic or sympathetic preganglionic pathways and that the reinnervation influences vasal function.

Upper thoracic sympathetic neuron responses to input from urinary bladder afferents

1983 ◽  
Vol 245 (3) ◽  
pp. R311-R320 ◽  
Author(s):  
R. Schondorf ◽  
W. Laskey ◽  
C. Polosa
Keyword(s):  
Electrical Stimulation ◽  
Urinary Bladder ◽  
Sympathetic Neuron ◽  
Neural Circuitry ◽  
Pelvic Nerve ◽  
Hypogastric Nerve ◽  
Bladder Afferents ◽  
Cervical Sympathetic ◽  
Upper Thoracic ◽  
Stimulation Of

The aim of the present study was to evaluate the organization of neural circuitry responsible for the intersegmental transmission of input from urinary bladder afferents to sympathetic preganglionic neurons (SPNs). The electrical activity of SPNs was recorded from axons of the cervical sympathetic trunk in anesthetized central nervous system (CNS)-intact and in unanesthetized midcollicular-decerebrate or acute C1 spinal cats. In all three preparations, tonically active SPNs were excited or inhibited by 1) electrical stimulation of myelinated afferents of the pelvic or hypogastric nerve, both of which contain bladder afferents, and 2) spontaneous contraction or distension of the urinary bladder. The SPN responses to bladder distension were abolished by pelvic nerve section. A comparison of responses of SPNs in CNS-intact and acute spinal animals to electrical stimulation of pelvic nerve afferents suggests that both propriospinal and supraspinal circuits are involved in the intersegmental transmission of input from bladder afferents to SPNs.

Effects of vasoactive intestinal peptide on canine prostatic contraction and secretion

1984 ◽  
Vol 247 (4) ◽  
pp. R701-R708 ◽  
Author(s):  
E. R. Smith ◽  
T. B. Miller ◽  
M. M. Wilson ◽  
M. C. Appel
Keyword(s):  
Heart Rate ◽  
Electrical Stimulation ◽  
Arterial Pressure ◽  
Vasoactive Intestinal Peptide ◽  
Ganglion Cells ◽  
Nerve Fibers ◽  
Hypogastric Nerve ◽  
Autonomic Ganglion ◽  
Anesthetized Dogs ◽  
Stimulation Of

Vasoactive intestinal peptide (VIP)-like immunoreactivity was found in intrinsic autonomic ganglion cells and nerve fibers located at the surface and within the canine prostate. In anesthetized dogs, porcine VIP (100–3,000 ng/kg iv) decreased arterial pressure and increased heart rate but did not result in the release of fluid from the prostate, indicating that VIP neither contracted glandular smooth muscle to expel fluid nor provoked secretion. Intravenous infusions of VIP at 10, 50, 100, and/or 200 ng X kg-1 X min-1 produced dose-related decreases in arterial pressure, increases in heart rate, and potentiation of the secretory response to the intravenous administration of pilocarpine and to electrical stimulation of the hypogastric nerves at 2 but not at 20 Hz. VIP at 1 microgram/ml neither contracted isolated strips of prostate nor modified their contraction by norepinephrine. It is unlikely that VIP mediates hypogastric nerve-induced prostatic contraction or secretion in the dog, but VIP may serve as a neuromodulator of nerve-induced secretion.

Neural regulation of the vas deferens in the rat: an electrophysiological analysis

1992 ◽  
Vol 263 (2) ◽  
pp. R331-R338 ◽  
Author(s):  
S. C. Kolbeck ◽  
W. D. Steers
Keyword(s):  
Neural Control ◽  
Vas Deferens ◽  
Contractile Activity ◽  
Male Reproduction ◽  
Nerve Transection ◽  
Pelvic Nerve ◽  
Hypogastric Nerve ◽  
C Fibers ◽  
Electrophysiological Analysis ◽  
Stimulation Of

Electrophysiological analysis of the neural control of the vas deferens was performed in urethan-anesthetized rats. Intraluminal distension (0.2 ml/min) or electrical stimulation of hypogastric (threshold 1-5 V, 20 Hz) and pelvic nerves (2-5 V, 20 Hz) produced contractions of the vas deferens. Distension-evoked contractile activity was not abolished by nicotinic ganglionic blockade or ipsilateral hypogastric and pelvic nerve transection. Contractions following hypogastric nerve stimulation were abolished by prazosin, while pelvic nerve-evoked responses were partially blocked by atropine. Hypogastric nerve, pelvic nerve, and sympathetic chain stimulation evoked volleys with latencies of 10-30 ms in vasal nerves. Crude estimates for conduction velocities for these responses (less than 0.5 m/s) corresponded to activation of unmyelinated C-fibers. Stimulation of the dorsal nerve of the penis (DNP) (10-16 V, 10-40 Hz) or administration of 5-methoxy-N,N-dimethyltryptamine, both of which produce seminal emission, elicited reflex discharges in nerves to the vas deferens. Hypogastric nerve but not pelvic nerve transection abolished both spontaneous and evoked (105- to 380-ms latency) reflex activity. These experiments provide insight into the organization of afferents in the DNP and efferents conveyed by autonomic pathways that regulate male reproduction.

Electrical stimulation of retinal neurons in epiretinal and subretinal configuration using a multicapacitor array

2012 ◽  
Vol 107 (10) ◽  
pp. 2742-2755 ◽  
Author(s):  
Max Eickenscheidt ◽  
Martin Jenkner ◽  
Roland Thewes ◽  
Peter Fromherz ◽  
Günther Zeck
Keyword(s):  
Electrical Stimulation ◽  
Ganglion Cells ◽  
Ex Vivo ◽  
Biophysical Model ◽  
Retinal Neurons ◽  
Direct Stimulation ◽  
Tungsten Electrode ◽  
Partial Restoration ◽  
Low Amplitude ◽  
Stimulation Of

Electrical stimulation of retinal neurons offers the possibility of partial restoration of visual function. Challenges in neuroprosthetic applications are the long-term stability of the metal-based devices and the physiological activation of retinal circuitry. In this study, we demonstrate electrical stimulation of different classes of retinal neurons with a multicapacitor array. The array—insulated by an inert oxide—allows for safe stimulation with monophasic anodal or cathodal current pulses of low amplitude. Ex vivo rabbit retinas were interfaced in either epiretinal or subretinal configuration to the multicapacitor array. The evoked activity was recorded from ganglion cells that respond to light increments by an extracellular tungsten electrode. First, a monophasic epiretinal cathodal or a subretinal anodal current pulse evokes a complex burst of action potentials in ganglion cells. The first action potential occurs within 1 ms and is attributed to direct stimulation. Within the next milliseconds additional spikes are evoked through bipolar cell or photoreceptor depolarization, as confirmed by pharmacological blockers. Second, monophasic epiretinal anodal or subretinal cathodal currents elicit spikes in ganglion cells by hyperpolarization of photoreceptor terminals. These stimuli mimic the photoreceptor response to light increments. Third, the stimulation symmetry between current polarities (anodal/cathodal) and retina-array configuration (epi/sub) is confirmed in an experiment in which stimuli presented at different positions reveal the center-surround organization of the ganglion cell. A simple biophysical model that relies on voltage changes of cell terminals in the transretinal electric field above the stimulation capacitor explains our results. This study provides a comprehensive guide for efficient stimulation of different retinal neuronal classes with low-amplitude capacitive currents.

Ca2+ handling properties of microsomal subfractions of rat vas deferens smooth muscle

1984 ◽  
Vol 62 (1) ◽  
pp. 76-79 ◽  
Author(s):  
A. K. Grover ◽  
C. Y. Kwan
Keyword(s):  
Smooth Muscle ◽  
Concentration Dependence ◽  
Vas Deferens ◽  
Membrane Vesicles ◽  
Rat Vas Deferens ◽  
Enzyme Marker ◽  
Isopycnic Centrifugation ◽  
Stimulation Of ◽  
Ph Profiles

The rat vas deferens smooth muscle microsomes on isopycnic centrifugation gave two fractions, namely F2 (15–30% sucrose) and F3 (30–40% sucrose), with comparable ATP-dependent azide-insensitive Ca2+-uptake capacities, although these fractions differed from each other in various enzyme marker activities. The fractions F2 and F3 also show similar pH profiles for the ATP-independent and ATP-dependent Ca2+ uptake, and similar ionized Ca2+-concentration dependence for the ATP-dependent Ca2+ uptake. However, the fractions F2 and F3 differ from each other in that: (a) F3 shows higher permeability to Ca2+, and (b) F3 shows higher stimulation of the ATP-dependent Ca2+ uptake by oxalate. The F3 fraction can also be used to obtain membrane vesicles loaded with Ca2+ oxalate in the presence of ATP. However, the yield of the Ca2+ oxalate enriched fraction is too low to permit their further characterization.

Electrical Pacing of the Paralyzed Human Larynx

1996 ◽  
Vol 105 (9) ◽  
pp. 689-693 ◽  
Author(s):  
Cheryl L. Rainey ◽  
Garrett D. Herzon ◽  
David L. Zealear ◽  
James L. Netterville ◽  
Robert H. Ossoff
Keyword(s):  
Electrical Stimulation ◽  
Vocal Fold ◽  
Abductor Muscle ◽  
Spontaneous Movement ◽  
Normal Side ◽  
Posterior Cricoarytenoid Muscle ◽  
Human Larynx ◽  
Posterior Cricoarytenoid ◽  
Electrical Pacing ◽  
Stimulation Of

This study represents the first attempt to electrically pace the paralyzed human larynx. The goal was to determine if electrical stimulation of the posterior cricoarytenoid muscle could produce functional abduction of the vocal fold in pace with inspiration. An external apparatus was used to sense inspiration and reanimate the unilaterally paralyzed larynx of a thyroplasty patient. Stimuli were delivered through a needle electrode to locate and pace the abductor muscle. The magnitude of electrically induced abduction was comparable to spontaneous movement on the normal side. The abduction was appropriately timed with inspiration; this finding demonstrated that this simple pacing system could effectively modulate stimulation with patient respiration.

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