scholarly journals Diversity in levels of intracellular total creatine and triglycerides in human skeletal muscles observed by1H-MRS

1999 ◽  
Vol 87 (6) ◽  
pp. 2068-2072 ◽  
Author(s):  
Jesús Rico-Sanz ◽  
E. Louise Thomas ◽  
Gabriele Jenkinson ◽  
Šárka Mierisová ◽  
Richard Iles ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Tibialis Anterior ◽  
Skeletal Muscles ◽  
Fiber Type ◽  
Resonance Spectroscopy ◽  
Fiber Type Composition ◽  
Type Composition ◽  
Total Creatine ◽  
Human Skeletal Muscles

We used1H-magnetic resonance spectroscopy to noninvasively determine total creatine (TCr), choline-containing compounds (Cho), and intracellular (IT) and extracellular (between-muscle fibers) triglycerides (ET) in three human skeletal muscles. Subjects' ( n = 15 men) TCr concentrations in soleus [Sol; 100.2 ± 8.3 (SE) mmol/kg dry wt] were lower ( P < 0.05) than those in gastrocnemius (Gast; 125.3 ± 9.2 mmol/kg dry wt) and tibialis anterior (TA; 123.7 ± 8.8 mmol/kg dry wt). The Cho levels in Sol (35.8 ± 3.6 mmol/kg dry wt) and Gast (28.5 ± 3.5 mmol/kg dry wt) were higher ( P < 0.001 and P < 0.01, respectively) compared with TA (13.6 ± 2.4 mmol/kg dry wt). The IT values were found to be 44.8 ± 4.6 and 36.5 ± 4.2 mmol/kg dry wt in Sol and Gast, respectively. The IT values of TA (24.5 ± 4.5 mmol/kg dry wt) were lower than those of Sol ( P < 0.01) and Gast ( P < 0.05). There were no differences in ET [116.0 ± 11.2 (Sol), 119.1 ± 18.5 (Gast), and 91.4 ± 19.2 mmol/kg dry wt (TA)]. It is proposed that the differences in metabolite levels may be due to the differences in fiber-type composition and deposition of metabolites due to the adaptation of different muscles during locomotion.

In Vitro sup 31 Phosphorus-Magnetic Resonance Spectroscopy of Muscle Extracts in Malignant Hyperthermia-susceptible Patients

1996 ◽  
Vol 84 (5) ◽  
pp. 1077-1082 ◽  
Author(s):  
Jean-Francois Payen ◽  
Nathalie Fouilhe ◽  
Ernest Sam-Lai ◽  
Chantal Remy ◽  
Roger Dupeyre ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Malignant Hyperthermia ◽  
Inorganic Phosphate ◽  
Fiber Type ◽  
Resonance Spectroscopy ◽  
Phosphorus Magnetic Resonance Spectroscopy ◽  
Malignant Hyperthermia Susceptible ◽  
Type Composition

Background It was recently suggested that malignant hyperthermia-susceptible (MHS) patients could have an elevated peak of phosphodiesters in leg muscles using in vivo phosphorus magnetic resonance spectroscopy. In the current study, analysis of the phosphodiesters of muscle extracts of MHS and malignant hyperthermia-negative patients was performed using in vitro phosphorus magnetic resonance spectroscopy to chemically identify and to compare the muscle concentrations of water-soluble compounds between the two groups with respect to the muscle fiber type composition. Methods Perchloric acid extracts of the vastus medialis muscle of seven MHS patients and ten malignant hyperthermia-negative patients on the basis of the European malignant hyperthermia contracture test were subjected to in vitro phosphorus magnetic resonance spectroscopy carried out at 9.4 T. In addition, chemical identification of the phosphodiester region and histologic examination of the muscle specimens were performed. Results The peak in the phosphodiester region was assigned to glycerophosphorylcholine. Muscle perchloric acid extracts of MHS patients had a significantly (P &lt; 0.05) higher glycerophosphorylcholine to the sum of phosphocreatine and inorganic phosphate (glycerophosphorylcholine/ [phosphocreatine +inorganic phosphate]) value than those of malignant hyperthermia-negative patients. Neither a difference in the fiber type composition between the two groups nor any specific myopathy were found. Conclusions In the absence of histologic differences between muscle specimens of MHS and malignant hyperthermia-negative patients, these results could suggest that glycerophosphorylcholine could be a marker of an impairment in the phospholipid metabolism in the skeletal muscle of MHS patients.

Downhill Running Excessive Training Inhibits Hypertrophy in Mice Skeletal Muscles with Different Fiber Type Composition

2015 ◽  
Vol 231 (5) ◽  
pp. 1045-1056 ◽  
Author(s):  
Alisson L. da Rocha ◽  
Bruno C. Pereira ◽  
José R. Pauli ◽  
Claudio T. de Souza ◽  
Giovana R. Teixeira ◽  
...  
Keyword(s):  
Skeletal Muscles ◽  
Fiber Type ◽  
Fiber Type Composition ◽  
Downhill Running ◽  
Type Composition

Phosphorus nuclear magnetic resonance spectroscopy of cardiac and skeletal muscles

1982 ◽  
Vol 242 (5) ◽  
pp. H729-H744 ◽  
Author(s):  
J. S. Ingwall
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Nuclear Magnetic Resonance Spectroscopy ◽  
Skeletal Muscles ◽  
Creatine Phosphate ◽  
Resonance Spectroscopy ◽  
Nmr Spectrum ◽  
Phosphorus Containing ◽  
Nuclear Magnetic

Phosphorus nuclear magnetic resonance spectroscopy (P-31 NMR) has been used to assess dynamic aspects of the metabolism of phosphorus-containing compounds in intact cells, organs, and animals. This review describes the NMR experiment and the kinds of information the P-31 NMR spectrum provides for intact, functioning cardiac and skeletal muscles. The P-31 NMR spectrum not only identifies which phosphorus-containing compounds are present in high concentration, namely adenosine 5'-triphosphate (ATP) and creatine phosphate, but also provides information about their chemical environment (including pH) and intracellular distribution. The method is quantitative and nondestructive and permits repetitive measurements in an intact functioning organ. For the perfused heart, it is possible to manipulate the chemical and gaseous composition of the perfusate and to define the effects of, for example, ischemia and reperfusion on the metabolism of ATP and creatine phosphate in the same sample. Using saturation-transfer NMR techniques, it is also possible to measure rates of certain reactions, including creatine kinase and adenylate kinase, in the intact cell. NMR can also be used as an imaging modality.

scholarly journals Magnetic resonance spectroscopy analysis of intramyocellular lipid composition in lipodystrophic patients and athletes

2018 ◽  
Author(s):  
David B Savage ◽  
Laura Watson ◽  
Katie Carr ◽  
Claire Adams ◽  
Soren Brage ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Tibialis Anterior ◽  
Saturation Index ◽  
Resonance Spectroscopy ◽  
Intramyocellular Lipid ◽  
Age And Gender ◽  
Insulin Resistant ◽  
And Gender

AbstractContextParadoxically, intramyocellular lipid (IMCL) accumulation has been linked to both insulin-resistant and to insulin-sensitive (athletes) states. The composition of this lipid store is unknown in these states.Design and MethodsWe used a recently validated and potentially widely applicable 1H magnetic resonance spectroscopy method to compare the compositional saturation index (CH2:CH3 ratio) and concentration independent of composition (CH3) of intramyocellular lipid in the soleus and tibialis anterior muscles of 16 female insulin-resistant lipodystrophic patients with that of age- and gender-matched athletes (n=14) and healthy controls (n = 41).Main OutcomeIMCL compositional saturation index (CH2:CH3 ratio).ResultsThe IMCL CH2:CH3 ratio was significantly higher in both muscles of the lipodystrophic patients compared with age- and gender-matched controls but not compared to athletes. IMCL CH2:CH3 was dependent on IMCL concentration in the controls and after adjusting the composition index for quantity (CH2:CH3adj) was able to distinguish patients from athletes. With groups pooled, this CH2:CH3adj marker had the strongest relation to insulin resistance (HOMA-IR) compared to other measures of lipid concentration and composition, especially in the soleus muscle. Contrary to the ‘athlete’s paradox’, IMCL in athletes was similar in tibialis anterior (p>0.05) and significantly lower in the soleus (p < 0.004) compared to both controls and patients.ConclusionsThe IMCL saturation index adjusted for quantity, which likely reflects accumulation of saturated IMCL, is more closely associated with insulin resistance than concentration alone.

scholarly journals Longitudinal Evaluation of Magnetic Resonance Spectroscopy Metabolites as Biomarkers in Huntingtons Disease

2021 ◽  
Author(s):  
Alexander J Lowe ◽  
Filipe B Rodrigues ◽  
Marzenna Arridge ◽  
Eileanoir B Johnson ◽  
Rachel I Scahill ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Disease Progression ◽  
Prognostic Value ◽  
Metabolite Concentration ◽  
Resonance Spectroscopy ◽  
Time Points ◽  
Gene Expansion ◽  
Total Creatine

Magnetic resonance spectroscopy (MRS) is a non-invasive method of exploring cerebral metabolism. In Huntingtons disease, altered MRS-determined concentrations of several metabolites have been described; however, findings are often discrepant and longitudinal studies of metabolite trajectory are lacking. MRS metabolites may represent a valuable source of biomarkers, thus their relationship with established biofluid and structural imaging markers of disease progression require further exploration to assess prognostic value and elucidate biochemical pathways associated with neurodegeneration. In a prospective single-site controlled cohort study with standardised collection of CSF, blood, phenotypic and imaging data, we used MRS to evaluate metabolic profiles in the putamen of 56 participants at baseline (15 healthy controls, 15 premanifest and 26 manifest gene expansion carriers) and at 2-year follow-up. Intergroup differences and associations with established measures were assessed cross-sectionally using generalized linear models and partial correlation, controlling for age and CAG repeat length. We report no significant groupwise differences in metabolite concentration but found several metabolites to be associated with measures of disease progression; however, only two relationships were replicated across both time points, with total Creatine (creatine + phosphocreatine) and myo-inositol displaying significant associations with reduced caudate volume. Although relationships were observed between MRS metabolites and biofluid measures, these were not consistent across time points. To further assess prognostic value of the metabolites, we examined whether baseline MRS values, or rate of change, predicted subsequent change in established measures of disease progression. Several associations were found but were inconsistent across known indicators of disease progression. Finally, longitudinal mixed effects models, controlling for age, revealed no significant change in metabolite concentration over time in gene expansion carriers. Altogether, our findings show some interesting cross-sectional associations between select metabolites, namely total creatine and myo-inositol, and markers of disease progression, potentially highlighting the proposed roles of neuroinflammation and metabolic dysfunction in disease pathogenesis. However, the absence of group differences, inconsistency between baseline and follow-up, and lack of clear longitudinal change over two years suggests that MRS metabolites have limited potential as biomarkers in Huntingtons disease.

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