Effects of swimming exercise on red blood cell  rheology in trained and untrained rats

Red blood cell (RBC) mechanical properties were investigated after swimming exercise in trained and untrained rats. A group of rats was trained for 6 wk (60 min swimming, daily), and another group was kept sedentary. Blood samples were obtained either within 5 min or 24 h after 60 min swimming in both groups. In the untrained rats, the RBC aggregation index decreased to 2.60 ± 0.4 immediately after exercise from a control value of 6.73 ± 0.18 ( P < 0.01), whereas it increased to 13.13 ± 0.66 after 24 h ( P < 0.01). RBC transit time through 5-μm pores increased to 3.53 ± 0.16 ms within 5 min after the exercise from a control value of 2.19 ± 0.07 ms ( P < 0.005). A very significant enhancement (166%) in RBC lipid peroxidation was detected only after 24 h. In the trained group, the alterations in all these parameters were attenuated; there was a slight, transient impairment in RBC deformability (transit time = 2.64 ± 0.13 ms), and lipid peroxidation was found to be unchanged. These findings suggest that training can significantly limit the hemorheological alterations related to a given bout of exercise. Whether this effect is secondary to the training-induced reduction in the degree of metabolic and/or hormonal perturbation remains to be determined.

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The Effect of Glucose and Poloxamer 188 on Red-Blood-Cell Aggregation

Metabolites ◽

10.3390/metabo11120886 ◽

2021 ◽

Vol 11 (12) ◽

pp. 886

Author(s):

Alicja Szołna-Chodór ◽

Bronisław Grzegorzewski

Keyword(s):

Mechanical Properties ◽

Blood Cell ◽

Red Blood Cell ◽

Cell Aggregation ◽

Aggregation Index ◽

Poloxamer 188 ◽

Red Blood Cell Aggregation ◽

Rbc Aggregation ◽

Effect Of Glucose ◽

Blood Cell Aggregation

Glucose metabolism disorders contribute to the development of various diseases. Numerous studies show that these disorders not only change the normal values of biochemical parameters but also affect the mechanical properties of blood. To show the influence of glucose and poloxamer 188 (P188) on the mechanical properties of a red-blood-cell (RBC) suspension, we studied the aggregation of the cells. To show the mechanisms of the mechanical properties of blood, we studied the effects of glucose and poloxamer 188 (P188) on red-blood-cell aggregation. We used a model in which cells were suspended in a dextran 70 solution at a concentration of 2 g/dL with glucose and P188 at concentrations of 0–3 g/dL and 0–3 mg/mL, respectively. RBC aggregation was determined using an aggregometer, and measurements were performed every 4 min for 1 h. Such a procedure enabled the incubation of RBCs in solution. The aggregation index determined from the obtained syllectograms was used as a measure of aggregation. Both the presence of glucose and that of P188 increased the aggregation index with the incubation time until saturation was reached. The time needed for the saturation of the aggregation index increased with increasing glucose and P188 concentrations. As the concentrations of these components increased, the joint effect of glucose and P188 increased the weakening of RBC aggregation. The mechanisms of the observed changes in RBC aggregation in glucose and P188 solutions are discussed.

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Peripartum Investigation of Red Blood Cell Properties in Women Diagnosed with Early-Onset Preeclampsia

Cells ◽

10.3390/cells10102714 ◽

2021 ◽

Vol 10 (10) ◽

pp. 2714

Author(s):

Beata Csiszar ◽

Gergely Galos ◽

Simone Funke ◽

Dora Kinga Kevey ◽

Matyas Meggyes ◽

...

Keyword(s):

Blood Cell ◽

Red Blood Cell ◽

Early Onset ◽

Light Transmission ◽

Erythrocyte Aggregation ◽

Infrared Light ◽

Aggregation Index ◽

Rbc Aggregation ◽

Early Onset Preeclampsia ◽

Gestational Age At Birth

We investigated peripartum maternal red blood cell (RBC) properties in early-onset preeclampsia (PE). Repeated blood samples were taken prospectively for hemorheological measurements at PE diagnosis (n = 13) or during 26–34 weeks of gestation in healthy pregnancies (n = 24), then at delivery, and 72 h postpartum. RBC aggregation was characterized by M index (infrared light transmission between the aggregated RBCs in stasis) and aggregation index (AI—laser backscattering from the RBC aggregates). We observed significantly elevated RBC aggregation (M index = 9.8 vs. 8.5; AI = 72.9% vs. 67.5%; p < 0.001) and reduced RBC deformability in PE (p < 0.05). A positive linear relationship was observed between AI and gestational age at birth in PE by regression analysis (R2 = 0.554; p = 0.006). ROC analysis of AI showed an AUC of 0.84 (0.68–0.99) (p = 0.001) for PE and indicated a cutoff of 69.4% (sensitivity = 83.3%; specificity = 62.5%), while M values showed an AUC of 0.75 (0.58–0.92) (p = 0.019) and indicated a cutoff of 8.39 (sensitivity = 90.9% and specificity = 50%). The predicted probabilities from the combination of AI and M variables showed increased AUC = 0.90 (0.79–1.00) (p < 0.001). Our results established impaired microcirculation in early-onset PE manifesting as deteriorated maternal RBC properties. The longer the pathologic pregnancy persists, the more pronounced the maternal erythrocyte aggregation. AI and M index could help in the prognostication of early-onset PE, but further investigations are warranted to confirm the prognostic role before the onset of symptoms.

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Microfluidic-based measurement of RBC aggregation and the ESR using a driving syringe system

Analytical Methods ◽

10.1039/c7ay02719b ◽

2018 ◽

Vol 10 (16) ◽

pp. 1805-1816 ◽

Cited By ~ 7

Author(s):

Yang Jun Kang

Keyword(s):

Blood Cell ◽

Erythrocyte Sedimentation Rate ◽

Microfluidic Device ◽

Sedimentation Rate ◽

Red Blood Cell ◽

Aggregation Index ◽

Erythrocyte Sedimentation ◽

System P ◽

Rbc Aggregation

The erythrocyte sedimentation rate (ESR) and red blood cell (RBC) aggregation in a driving syringe are simultaneously measured by quantifying blood press index (Ipress) and RBC aggregation index (IRA) in a microfluidic device.

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Detection and characterization of red blood cell (RBC) aggregation with photoacoustics

10.1117/12.908685 ◽

2012 ◽

Cited By ~ 2

Author(s):

Eno Hysi ◽

Ratan K. Saha ◽

Min Rui ◽

Michael C. Kolios

Keyword(s):

Blood Cell ◽

Red Blood Cell ◽

Rbc Aggregation

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Effects of oxidative stress on red blood cell rheology in sickle cell patients

British Journal of Haematology ◽

10.1111/bjh.12912 ◽

2014 ◽

Vol 166 (4) ◽

pp. 601-606 ◽

Cited By ~ 38

Author(s):

Régine Hierso ◽

Xavier Waltz ◽

Pierre Mora ◽

Marc Romana ◽

Nathalie Lemonne ◽

...

Keyword(s):

Oxidative Stress ◽

Blood Cell ◽

Sickle Cell ◽

Red Blood Cell ◽

Cell Rheology

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Red blood cell rheology in sepsis

Applied Physiology in Intensive Care Medicine ◽

10.1007/3-540-37363-2_35 ◽

2007 ◽

pp. 229-238

Author(s):

M. Piagnerelli ◽

K. Zouaoui Boudjeltia ◽

M. Vanhaeverbeek ◽

J. -L. Vincent

Keyword(s):

Blood Cell ◽

Red Blood Cell ◽

Cell Rheology

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Red Blood Cell Aggregation-Associated Dietary Pattern Predicts Hyperlipidemia and Metabolic Syndrome

Nutrients ◽

10.3390/nu10081127 ◽

2018 ◽

Vol 10 (8) ◽

pp. 1127 ◽

Cited By ~ 4

Author(s):

Pei Lin ◽

Chun-Chao Chang ◽

Kuo-Ching Yuan ◽

Hsing-Jung Yeh ◽

Sheng-Uei Fang ◽

...

Keyword(s):

Metabolic Syndrome ◽

Blood Cell ◽

Red Blood Cell ◽

Dietary Patterns ◽

Dietary Pattern ◽

Iron Status ◽

Transferrin Saturation ◽

Dietary Factors ◽

Reduced Rank Regression ◽

Rbc Aggregation

Red blood cell (RBC) aggregation and iron status are interrelated and strongly influenced by dietary factors, and their alterations pose a great risk of dyslipidemia and metabolic syndrome (MetS). Currently, RBC aggregation-related dietary patterns remain unclear. This study investigated the dietary patterns that were associated with RBC aggregation and their predictive effects on hyperlipidemia and MetS. Anthropometric and blood biochemical data and food frequency questionnaires were collected from 212 adults. Dietary patterns were derived using reduced rank regression from 32 food groups. Adjusted linear regression showed that hepcidin, soluble CD163, and serum transferrin saturation (%TS) independently predicted RBC aggregation (all p < 0.01). Age-, sex-, and log-transformed body mass index (BMI)-adjusted prevalence rate ratio (PRR) showed a significant positive correlation between RBC aggregation and hyperlipidemia (p-trend < 0.05). RBC aggregation and iron-related dietary pattern scores (high consumption of noodles and deep-fried foods and low intake of steamed, boiled, and raw food, dairy products, orange, red, and purple vegetables, white and light-green vegetables, seafood, and rice) were also significantly associated with hyperlipidemia (p-trend < 0.05) and MetS (p-trend = 0.01) after adjusting for age, sex, and log-transformed BMI. Our results may help dieticians develop dietary strategies for preventing dyslipidemia and MetS.

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