scholarly journals Novel serum biomarkers for erythropoietin use in humans: a proteomic approach

2011 ◽  
Vol 110 (1) ◽  
pp. 149-156 ◽  
Author(s):  
Britt Christensen ◽  
Lucila Sackmann-Sala ◽  
Diana Cruz-Topete ◽  
Jens Otto L. Jørgensen ◽  
Niels Jessen ◽  
...  
Keyword(s):  
Serum Proteins ◽  
Endurance Performance ◽  
Serum Biomarkers ◽  
Arterial Oxygen ◽  
Total Serum ◽  
Two Dimensional Gel Electrophoresis ◽  
Arterial Oxygen Content ◽  
Serum Haptoglobin ◽  
Proteomic Approach ◽  
Male Subjects

Erythropoietin (Epo) is produced primarily in the kidneys upon low blood oxygen availability and stimulates erythropoiesis in the bone marrow. Recombinant human Epo (rHuEpo), a drug developed to increase arterial oxygen content in patients, is also illicitly used by athletes to improve their endurance performance. Therefore, a robust and sensitive test to detect its abuse is needed. The aim of the present study was to investigate potential human serum biomarkers of Epo abuse employing a proteomic approach. Eight healthy male subjects were injected subcutaneously with rHuEpo (5,000 IU) every second day for a 16-day period. Serum was collected before starting the treatment regime and again at days 8 and 16 during the treatment period. Samples were homogenized and proteins separated by two-dimensional gel electrophoresis (2DE). Spots that changed significantly in response to rHuEpo treatment were identified by mass spectrometry. Both the number of reticulocytes and erythrocytes increased throughout the study, leading to a significant increase in hematocrit and hemoglobin content. In addition, transferrin levels increased but the percentage of iron bound to transferrin and ferritin levels decreased. Out of 97 serum proteins, seven were found to decrease significantly at day 16 compared with pre-Epo administration, and were identified as four isoforms of haptoglobin, two isoforms of transferrin, and a mixture of hemopexin and albumin. In support, total serum haptoglobin levels were found to be significantly decreased at both days 8 and 16. Thus a 2DE proteomic approach for discovery of novel markers of Epo action appears feasible.

Haemoglobin, Blood Volume, Cardiac Function, and Aerobic Power

1999 ◽  
Vol 24 (1) ◽  
pp. 54-65 ◽  
Author(s):  
Norman Gledhill ◽  
Darren Warburton ◽  
Veronica Jamnik
Keyword(s):  
Cardiac Output ◽  
Aerobic Power ◽  
Endurance Performance ◽  
Endurance Athletes ◽  
Aerobic Performance ◽  
Arterial Oxygen ◽  
Blood Doping ◽  
Arterial Oxygen Content ◽  
The Difference ◽  
Key Words Blood

Alterations in [Hb], which are mediated through changes in arterial oxygen content, and alterations in BV, which are mediated through changes in cardiac output [Formula: see text] have a significant effect on both [Formula: see text] and aerobic performance. If BV is held constant, a decrease in [Hb] (anaemia) causes a decrease in [Formula: see text] and aerobic performance, while an increase in [Hb] (blood doping) causes an increase in [Formula: see text] and aerobic performance. If [Hb] is held constant, an increase in BV can cause an increase in both [Formula: see text] and aerobic performance, while a decrease in BV can cause a decrease in both [Formula: see text] and aerobic performance. In addition, an increase in BV can compensate for moderate reductions in [Hb] through increases in [Formula: see text], allowing [Formula: see text] to remain unchanged or even increase. Also, a large portion of the difference in the enhanced cardiovascular function of endurance athletes is due to their high BV and the resultant enhancement of diastolic function. Hence, optimizing both [Hb] and BV is a very important consideration for endurance performance. Key words: blood doping, sport anemia, stroke volume

scholarly journals The Effect of Dietary Intake and Nutritional Status on Anthropometric Development and Systemic Inflammation: An Observational Study

2021 ◽  
Vol 18 (11) ◽  
pp. 5635
Author(s):  
Roxana Maria Martin-Hadmaș ◽  
Ștefan Adrian Martin ◽  
Adela Romonți ◽  
Cristina Oana Mărginean
Keyword(s):  
Serum Proteins ◽  
Caloric Intake ◽  
Saturated Fat ◽  
The Body ◽  
Total Serum ◽  
Fat Intake ◽  
Trans Fats ◽  
Study Objective ◽  
Inflammatory Status ◽  
Total Fat

(1) Background: Daily caloric intake should aim to reduce the risk of obesity or poor anthropometric development. Our study objective was to analyze the association between food consumption, inflammatory status and anthropometric development; (2) Methods: We performed a prospective observational analytical research during September 2020 and April 2021 on a group of 160 healthy subjects, aged between 6 and 12 years old, by analyzing food ingestion, the basal metabolic rate, anthropometric development and the inflammatory status; (3) Results: IL-6 was significantly correlated to the sum of skinfolds, along with both serum proteins and triglycerides. The skin folds were significantly correlated with the caloric intake and with total fat intake, next to saturated and trans fats. Unlike the skin folds, the body weight was significantly correlated with the caloric intake along with some vitamins, such as Vitamin A and Vitamin B12. Inactive mass increased with excessive folic acid, Vitamin E, Vitamin K and saturated fat intake; (4) Conclusions: The inflammatory status was influenced by the ingestion of micronutrients, total serum lipids and proteins. The anthropometric development was associated with the ingestion of carbohydrates, energy balance and energy intake. We can conclude that daily menu and nutrition imbalances can influence both the risk of obesity and the inflammatory status.

Blood flow to fetal organs as a function of arterial oxygen content

1979 ◽  
Vol 135 (5) ◽  
pp. 637-646 ◽  
Author(s):  
Louis L.H. Peeters ◽  
Roger E. Sheldon ◽  
M. Douglas Jones ◽  
Edgar L. Makowski ◽  
Giacomo Meschia
Keyword(s):  
Blood Flow ◽  
Oxygen Content ◽  
Arterial Oxygen ◽  
Arterial Oxygen Content ◽  
Fetal Organs

Muscle Weight Relationship to Serum Proteins, Hemocytes, and Hepatopancreas in the Lobster, Homarus americanus

1967 ◽  
Vol 24 (11) ◽  
pp. 2339-2354 ◽  
Author(s):  
James E. Stewart ◽  
John W. Cornick ◽  
Diane M. Foley ◽  
M. F. Li ◽  
C. M. Bishop
Keyword(s):  
Serum Protein ◽  
Physiological Condition ◽  
Serum Proteins ◽  
Homarus Americanus ◽  
Total Serum Protein ◽  
Total Serum ◽  
Experimental Conditions ◽  
Muscle Weight ◽  
Beef Liver ◽  
The Relationship

Total serum protein values, hemocyte numbers, and muscle weights were determined for 216 intermolt lobsters immediately after their capture, and for 230 others held captive under a variety of dietary and environmental conditions. Average muscle values ranged from approximately 13% to the more normal 20–25% of the live animals' weight, depending upon experimental conditions. The total serum protein up to a level of 55 mg/ml was shown to be a reliable indicator of muscle weights, although the relationship was not identical for all lobster groups. It appeared to be modified chiefly by the areas from which the different groups were taken. Diet was more important than the temperatures (5 to 14 C) in affecting changes in muscle and serum protein values. Starvation caused a greater reduction (50 to 70%) in the size of the hepatopancreas than in the muscle. Histological examination of the hepatopancreatic tissue showed that the lipid content was markedly reduced upon starvation and that a degeneration of this organ was apparent for lobsters fed a beef liver and herring diet. Measurement of serum proteins would appear to be a useful technique in experiments on lobster nutrition and have value, within specified limits, for assessing the physiological condition of wild lobsters.

Cardiovascular and Respiratory Changes During Heat Stress in Rainbow Trout (Salmo Gairdneri)

1973 ◽  
Vol 59 (2) ◽  
pp. 323-338 ◽  
Author(s):  
ALAN G. HEATH ◽  
G. M. HUGHES
Keyword(s):  
Oxygen Content ◽  
Oxygen Demand ◽  
Heart Beat ◽  
Arterial Oxygen ◽  
Salmo Gairdneri ◽  
Steady Increase ◽  
Limiting Factor ◽  
Arterial Oxygen Content ◽  
Ventilatory Frequency ◽  
Increasing Temperature

1. Trout were subjected to a steady increase in water temperature (1.5 °C/h) from 15 °C until death occurred, while several respiratory and cardiovascular parameters were monitored. 2. Oxygen consumption increased during the warming (Q10 = 2.35 between 16 and 20 °C). At the higher temperatures the increase was more marked (Q10 =4.96 between 20 and 26 °C). 3. Ventilatory frequency increased during the rising temperature with a general levelling off observed above 23 °C. The amplitude of the pressure changes in the buccal and opercular cavities increased more than did the ventilatory frequency. Further analysis of the differential pressure across the gills suggests that the adjustment of respiratory pumping to the increased oxygen demand is predominantly in the volume pumped per stroke (cycle). 4. Heart rate rose steadily with the increasing temperature until about 24-25 °C, when a bradycardia usually became evident. Synchrony between the heart beat and the respiratory pumps was observed in some preparations at the higher temperatures. 5. Blood pressure increases during the warming were more marked in the ventral aorta than in the dorsal aorta. At the highest temperatures, abnormal cardiac cycles were frequently observed. 6. Arterial oxygen content declined slightly during warming and venous oxygen content dropped to zero above 23 °C. 7. It is suggested that cardiovascular adjustments may be a limiting factor in this type of stress.

Effect of multiple oral doses of androgenic anabolic steroids on endurance performance and serum indices of physical stress in healthy male subjects

2006 ◽  
Vol 98 (4) ◽  
pp. 329-340 ◽  
Author(s):  
Norbert Baume ◽  
Yorck Olaf Schumacher ◽  
Pierre-Edouard Sottas ◽  
Carlo Bagutti ◽  
Michel Cauderay ◽  
...  
Keyword(s):  
Anabolic Steroids ◽  
Endurance Performance ◽  
Physical Stress ◽  
Healthy Male ◽  
Androgenic Anabolic Steroids ◽  
Oral Doses ◽  
Male Subjects

scholarly journals Comparative Secretome Analyses of Three Bacillus anthracis Strains with Variant Plasmid Contents

2005 ◽  
Vol 73 (6) ◽  
pp. 3646-3658 ◽  
Author(s):  
Janine M. Lamonica ◽  
MaryAnn Wagner ◽  
Michel Eschenbrenner ◽  
Leanne E. Williams ◽  
Tabbi L. Miller ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Protective Antigen ◽  
Isocitrate Lyase ◽  
Lethal Factor ◽  
Protein A ◽  
Surface Protein ◽  
Computer Assisted ◽  
Two Dimensional Gel Electrophoresis ◽  
Peptide Mass ◽  
Proteomic Approach

ABSTRACT Bacillus anthracis, the causative agent of anthrax, secretes numerous proteins into the extracellular environment during infection. A comparative proteomic approach was employed to elucidate the differences among the extracellular proteomes (secretomes) of three isogenic strains of B. anthracis that differed solely in their plasmid contents. The strains utilized were the wild-type virulent B. anthracis RA3 (pXO1+ pXO2+) and its two nonpathogenic derivative strains: the toxigenic, nonencapsulated RA3R (pXO1+ pXO2−) and the totally cured, nontoxigenic, nonencapsulated RA3:00 (pXO1− pXO2−). Comparative proteomics using two-dimensional gel electrophoresis followed by computer-assisted gel image analysis was performed to reveal unique, up-regulated, or down-regulated secretome proteins among the strains. In total, 57 protein spots, representing 26 different proteins encoded on the chromosome or pXO1, were identified by peptide mass fingerprinting. S-layer-derived proteins, such as Sap and EA1, were most frequently observed. Many sporulation-associated enzymes were found to be overexpressed in strains containing pXO1+. This study also provides evidence that pXO2 is necessary for the maximal expression of the pXO1-encoded toxins lethal factor (LF), edema factor (EF), and protective antigen (PA). Several newly identified putative virulence factors were observed; these include enolase, a high-affinity zinc uptake transporter, the peroxide stress-related alkyl hydroperoxide reductase, isocitrate lyase, and the cell surface protein A.

Saturation of arterial blood with oxygen during maximal exercise

1964 ◽  
Vol 19 (2) ◽  
pp. 284-286 ◽  
Author(s):  
Loring B. Rowell ◽  
Henry L. Taylor ◽  
Yang Wang ◽  
Walter S. Carlson
Keyword(s):  
Oxygen Saturation ◽  
Arterial Oxygen Saturation ◽  
Arterial Oxygen ◽  
Oxygen Intake ◽  
Arterial Oxygen Content ◽  
Good Physical Condition ◽  
Arterial Blood ◽  
Sedentary Group ◽  
Arterial Desaturation ◽  
Sufficient Intensity

The per cent saturation of the arterial blood with oxygen was examined in four men before and during the last 15 sec of a 3-min run of sufficient intensity to elicit a maximal oxygen intake. The measurements were repeated after a 3-month period of intensive conditioning for middle distance running and in a group of four athletes in good physical condition. The per cent saturation in the sedentary group was 95.8 at rest and 93.4 during exhausting exercise; after conditioning the similar figures were 95.4 and 91.4 and, finally, the athletes showed a per cent saturation of 85.2 during the heavy work. The arterial oxygen content during exhausting work was found to be 20.12 ml/100 ml blood in the sedentary group before training, 19.02 after conditioning, and 18.18 in the group of athletes. It is concluded that, in athletes who are well conditioned and pushing themselves close to the limit of their capacity, arterial desaturation can take place. athletic conditioning and arterial oxygen saturation; arterial desaturation in athletes; ventilation and arterial desaturation; oxygen intake and arterial oxygen saturation Submitted on August 5, 1963

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