Similar changes of gene expression in human skeletal muscle after resistance exercise and multiple fine needle biopsies

2012 ◽  
Vol 112 (2) ◽  
pp. 289-295 ◽  
Author(s):  
Birgit Friedmann-Bette ◽  
Fides Regina Schwartz ◽  
Holger Eckhardt ◽  
Rudolf Billeter ◽  
Gabriel Bonaterra ◽  
...  
Keyword(s):  
Gene Expression ◽  
Resistance Exercise ◽  
Glucose Transporter ◽  
Vastus Lateralis ◽  
Whole Body ◽  
Control Group ◽  
Marker Genes ◽  
Vastus Lateralis Muscle ◽  
Fine Needle ◽  
Lateralis Muscle

Repeated biopsy sampling from one muscle is necessary to investigate muscular adaptation to different forms of exercise as adaptation is thought to be the result of cumulative effects of transient changes in gene expression in response to single exercise bouts. In a crossover study, we obtained four fine needle biopsies from one vastus lateralis muscle of 11 male subjects (25.9 ± 3.8 yr, 179.2 ± 4.8 cm, 76.5 ± 7.0 kg), taken before (baseline), 1, 4, and 24 h after one bout of squatting exercise performed as conventional squatting or as whole body vibration exercise. To investigate if the repeated biopsy sampling has a confounding effect on the observed changes in gene expression, four fine needle biopsies from one vastus lateralis muscle were also taken from 8 male nonexercising control subjects (24.5 ± 3.7 yr, 180.6 ± 1.2 cm, 81.2 ± 1.6 kg) at the equivalent time points. Using RT-PCR, we observed similar patterns of change in the squatting as well as in the control group for the mRNAs of interleukin 6 (IL-6), IL-6 receptor, insulin-like growth factor 1, p21, phosphofructokinase, and glucose transporter in relation to the baseline biopsy. In conclusion, multiple fine needle biopsies obtained from the same muscle region can per se influence the expression of marker genes induced by an acute bout of resistance exercise.

Effect of needle biopsy from the vastus lateralis muscle on insulin-stimulated glucose metabolism in humans

1994 ◽  
Vol 267 (4) ◽  
pp. E544-E548 ◽  
Author(s):  
P. Holck ◽  
N. Porksen ◽  
M. F. Nielsen ◽  
B. Nyholm ◽  
J. F. Bak ◽  
...  
Keyword(s):  
Insulin Sensitivity ◽  
Glucose Metabolism ◽  
Glucose Uptake ◽  
Muscle Biopsy ◽  
Needle Biopsy ◽  
Vastus Lateralis ◽  
Whole Body ◽  
Vastus Lateralis Muscle ◽  
Lateralis Muscle ◽  
Glucose Output

To examine the cellular mechanisms behind conditions characterized by insulin resistance, the clamp technique is often combined with muscle biopsies. To test whether the trauma of a needle biopsy from the vastus lateralis muscle per se may influence insulin-stimulated glucose uptake, eight healthy subjects underwent two randomly sequenced hyperinsulinemic (insulin infusion rate: 0.6 mU.kg-1.min-1 for 150 min) euglycemic clamps with an interval of 4-6 wk. In one study (study B) a muscle biopsy (approximately 250 mg, i.e., larger than normal standard) was taken in the basal state just before the clamp procedure, whereas the other was a control study (study C). Insulin-stimulated glucose uptake was significantly reduced in study B (5.36 +/- 0.96 mg.kg-1.min-1) compared with study C (6.06 +/- 0.68 mg.kg-1.min-1; P < 0.05). Nonoxidative glucose disposal (indirect calorimetry) was decreased (2.81 +/- 1.08 vs. 3.64 +/- 1.34 mg.kg-1.min-1; P < 0.05), whereas glucose oxidation was unaltered. Likewise, endogenous glucose output ([3-3H]glucose) was identically suppressed during hyperinsulinemia. Circulating levels of epinephrine, glucagon, and growth hormone did not differ significantly in studies B and C. In contrast, plasma norepinephrine, serum cortisol, and free fatty acid rose after biopsy (P < 0.05). In conclusion, performance of a muscle biopsy may diminish insulin sensitivity by affecting nonoxidative glucose metabolism. This should be considered when assessing whole body insulin sensitivity after a percutaneous needle muscle biopsy.

The Effect of Aerobic Exercise Training on the Distribution of Succinate Dehydrogenase Activity Throughout Muscle Fibres

1998 ◽  
Vol 23 (1) ◽  
pp. 74-86 ◽  
Author(s):  
Philip D. Chilibeck ◽  
Gordon J. Bell ◽  
Teresa Socha ◽  
Tom Martin
Keyword(s):  
Endurance Training ◽  
Succinate Dehydrogenase ◽  
Vastus Lateralis ◽  
Aerobic Exercise Training ◽  
Control Group ◽  
Muscle Fibres ◽  
Vastus Lateralis Muscle ◽  
Type I ◽  
Lateralis Muscle ◽  
Subsarcolemmal Mitochondria

We evaluated the effect of endurance training (cycling 3 times per week for 12 weeks) on succinate dehydrogenase (SDH) activity in the subsarcolemmal (SS) and intermyofibrillar (IMF) regions of vastus lateralis muscle fibres in 7 individuals (4 females and 3 males). SDH activity of the SS region increased 9.4% and 12.8% in type I and II fibres, respectively (p < .05). SDH activity of the IMF region increased 4.7% and 6.7% in type I and II fibres, respectively (p < .05). This was less than the increase in the SS region (p < .O5). No significant changes were observed in a control group (4 females and 3 males). These data suggest that mitochondria in the SS and IMF regions of human vastus lateralis muscle fibres are sensitive to endurance training. The greater response in the SS region suggests that the metabolic requirements of SS mitochondria were stressed to a greater extent than IMF mitochondria with endurance training. Key words: subsarcolemmal mitochondria, intermyofibrillar mitochondria

scholarly journals Dose atrophy of vastus medialis obliquus and vastus lateralis exist in patients with patellofemoral pain syndrome

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Conglei Dong ◽  
Ming Li ◽  
Kuo Hao ◽  
Chao Zhao ◽  
Kang Piao ◽  
...  
Keyword(s):  
Vastus Lateralis ◽  
Pain Syndrome ◽  
Patellofemoral Pain Syndrome ◽  
Patellofemoral Pain ◽  
Control Group ◽  
Vastus Lateralis Muscle ◽  
Vastus Medialis ◽  
Muscle Area ◽  
Vastus Medialis Obliquus ◽  
Lateralis Muscle

Abstract Background Whether vastus medialis obliquus atrophy exists in patients with patellofemoral pain syndrome and whether the amount of atrophy differs between the vastus medialis obliquus and vastus lateralis muscles remain unknown. Materials From June 2016 to March 2019, 61 patients with patellofemoral pain syndrome were retrospectively included in the study group, and an age-, sex-, and body mass index-matched cohort of 61 patients with normal knees was randomly selected as the control group. All enrolled subjects had undergone CT scans in the supine position. The cross-sectional areas of the vastus medialis obliquus and the vastus lateralis muscle in the sections 0, 5, 10, 15, and 20 mm above the upper pole of the patella were measured, and the vastus medialis obliquus/vastus lateralis muscle area ratio was evaluated. Results In the study group, the vastus medialis obliquus areas and the vastus lateralis muscle areas in the sections that were 0, 5, 10, 15, and 20 mm above the upper pole of the patella were significantly smaller than the respective areas in the control group (P < 0.05). The vastus medialis obliquus/vastus lateralis muscle area ratio was significantly smaller at the upper pole of the patella (the section 0 mm above the upper pole of the patella) than the corresponding ratio in the control group (P < 0.05). No significant difference was noted between the two groups in the sections 5, 10, 15, and 20 mm above the upper pole of the patella (P > 0.05). Conclusion In patients with patellofemoral pain syndrome, vastus medialis obliquus and vastus lateralis muscle atrophy existed in sections 0–20 mm above the upper pole of the patella, compared with normal controls, and atrophy of the vastus medialis obliquus was more evident than that of the vastus lateralis muscle at the upper pole of the patella. These findings support the rationale for the use of general quadriceps exercise combined with vastus medialis obliquus strengthening exercise as part of the rehabilitation programme for the patients with patellofemoral pain syndrome.

Effect of training and detraining on skeletal muscle glucose transporter (GLUT4) content in rats

1992 ◽  
Vol 70 (9) ◽  
pp. 1286-1290 ◽  
Author(s):  
P. D. Neufer ◽  
M. H. Shinebarger ◽  
G. L. Dohm
Keyword(s):  
Skeletal Muscle ◽  
Protein Content ◽  
Glucose Transporter ◽  
Citrate Synthase ◽  
Treadmill Exercise ◽  
Vastus Lateralis ◽  
Vastus Lateralis Muscle ◽  
Transporter Protein ◽  
Lateralis Muscle ◽  
Training And Detraining

The aim of the present study was to examine the effects of treadmill exercise training and detraining on the skeletal muscle fiber type specific expression of the insulin-regulated glucose transporter protein (GLUT4) in rats. GLUT4 protein content was determined by Western and dot-blot analysis, using a polyclonal antibody raised against the carboxy-terminal peptide. Rats were sacrificed 24 h after the last training session. There were no significant changes in muscle GLUT4 after 1 day or 1 week of training. Six weeks of training increased GLUT4 protein content 1.4- to 1.7-fold (p < 0.05) over controls in the soleus and red vastus lateralis, whereas no significant change was evident in the white vastus lateralis muscle. GLUT4 protein content in both soleus and red vastus lateralis muscle returned to near control values after 7 days of detraining. Similar to GLUT4, citrate synthase activity showed no change after 1 day or 1 week of training, increased 1.8-fold over controls after 6 weeks of training, but returned to control values after 7 days detraining. These findings demonstrate that muscle GLUT4 protein is increased in rats with as little as 6 weeks of treadmill exercise training but that the adaptation is lost within 1 week of detraining. It is suggested that expression of the GLUT4 protein is coordinated with the well-documented adaptations in oxidative enzyme activity with endurance training and detraining.Key words: insulin-regulated glucose transporter protein, citrate synthase.

scholarly journals Aberrant REDD1-mTORC1 responses to insulin in skeletal muscle from Type 2 diabetics

2015 ◽  
Vol 309 (8) ◽  
pp. R855-R863 ◽  
Author(s):  
David L. Williamson ◽  
Cory M. Dungan ◽  
Abeer M. Mahmoud ◽  
Jacob T. Mey ◽  
Brian K. Blackburn ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Vastus Lateralis ◽  
Glycosylated Hemoglobin ◽  
Whole Body ◽  
Glucose Disposal ◽  
Control Group ◽  
Vastus Lateralis Muscle ◽  
Percent Change ◽  
Phosphorylated Akt

The objective of this study was to establish whether alterations in the REDD1-mTOR axis underlie skeletal muscle insensitivity to insulin in Type 2 diabetic (T2D), obese individuals. Vastus lateralis muscle biopsies were obtained from lean, control and obese, T2D subjects under basal and after a 2-h hyperinsulinemic (40 mU·m−2·min−1)-euglycemic (5 mM) clamp. Muscle lysates were examined for total REDD1, and phosphorylated Akt, S6 kinase 1 (S6K1), 4E-BP1, ERK1/2, and MEK1/2 via Western blot analysis. Under basal conditions [(-) insulin], T2D muscle exhibited higher S6K1 and ERK1/2 and lower 4E-BP1 phosphorylation ( P < 0.05), as well as elevations in blood cortisol, glucose, insulin, glycosylated hemoglobin ( P < 0.05) vs. lean controls. Following insulin infusion, whole body glucose disposal rates (GDR; mg/kg/min) were lower ( P < 0.05) in the T2D vs. the control group. The basal-to-insulin percent change in REDD1 expression was higher ( P < 0.05) in muscle from the T2D vs. the control group. Whereas, the basal-to-insulin percent change in muscle Akt, S6K1, ERK1/2, and MEK1/2 phosphorylation was significantly lower ( P < 0.05) in the T2D vs. the control group. Findings from this study propose a REDD1-regulated mechanism in T2D skeletal muscle that may contribute to whole body insulin resistance and may be a target to improve insulin action in insulin-resistant individuals.

scholarly journals The Vastus Medialis obliquus and The Vastus Lateralis Muscle Atrophy is Existed in Patients with Patellofemoral Pain Syndrome

2020 ◽  
Author(s):  
Conglei Dong ◽  
Ming Li ◽  
Kuo Hao ◽  
Chao Zhao ◽  
Kang Piao ◽  
...  
Keyword(s):  
Vastus Lateralis ◽  
Rehabilitation Program ◽  
Area Ratio ◽  
Control Group ◽  
Vastus Lateralis Muscle ◽  
Study Group ◽  
Vastus Medialis ◽  
Cross Sectional ◽  
Vastus Medialis Obliquus ◽  
Lateralis Muscle

Abstract Background: whether the vastus medialis obliquus (VMO) atrophy exists in patients with PFPS and whether the amount of atrophy differs between the VMO and vastus lateralis muscle (VLM) is still obscure. Materials and methods: From June 2016 to March 2019, 61 patients with PFPS were collected into the study group, and an age, sex, and body mass index (BMI) matched cohort of 61 patients with normal knees were randomly selected into the control group. All enrolled subjects had undergone computed Tomography (CT) scans in the supine position. The cross-sectional area of the VMO and VLM in the sections of 0, 5, 10, 15, 20 mm above the upper pole of the patella were measured, and VMO/VLM area ratio were evaluated as well. Results: In the study group and the control group, the VMO area in the section that 0, 5, 10, 15, 20 mm above the upper pole of the patella were 732.64±306.43 mm2 and 941.66±366.83 mm2 (P<0.001), 876.32±341.47 mm2 and 1119.6±405.01 mm2 (P<0.001), 1039.31±410.21 mm2 and 1302.75±425.14 mm2 (P<0.001), 1178.26±449.10 mm2 and 1496.67±474.70 mm2 (P<0.001), 1289.78±487.78 mm2 and 1643.33±507.08 mm2 (P<0.001); the VLM area in the section that 0, 5, 10, 15, 20 mm above the upper pole of the patella were 127.61±66.74 mm2 and 192.2±152.40 mm2 (P=0.003), 183.47±85.41 mm2 and 262.55±187.98 mm2 (P=0.004), 250.66±133.70 mm2 and 352.35±291.96 mm2 (P=0.015), 326.06±139.94 mm2 and 466.27±343.11 mm2 (P=0.013), 574.19±390.00 mm2 (P=0.005); the VMO/ VLM area ratio in the section that 0, 5, 10, 15, 20 mm above the upper pole of the patella were 0.83±0.11 and 7.44±5.13 (P<0.001), 5.37±2.49 and 6.32±4.69 (P=0.168), 4.64±2.43 and 4.15±1.94 (P=0.554), 3.90±1.55 and 3.96±1.66 (P=0.434), 3.42±1.36 and 3.48±1.62 (P=0.826).Conclusion: In patients with PFPS, the VMO and VLM atrophy was existed in the section of 0-20 mm above the upper pole of the patella in comparison with normal people; and the atrophy of the VMO was more evident than that of the VLM in the section that 0-5 mm above the upper pole of the patella. These findings support the rationale for use of general quadriceps exercise combined with VMO strengthening exercise as part of rehabilitation program for patients with PFPS.

scholarly journals Gene Expression Profiling in Vastus Lateralis Muscle During an Acute Exacerbation of COPD

2010 ◽  
Vol 25 (4-5) ◽  
pp. 491-500 ◽  
Author(s):  
Tim Crul ◽  
Dries Testelmans ◽  
Martijn Spruit ◽  
Thierry Troosters ◽  
Rik Gosselink ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Acute Exacerbation ◽  
Expression Profiling ◽  
Vastus Lateralis ◽  
Vastus Lateralis Muscle ◽  
Acute Exacerbation Of Copd ◽  
Lateralis Muscle ◽  
Exacerbation Of Copd
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