An electrically evoked slow potential of the frog's retina. II. Identification with PII component of electroretinogram

1975 ◽  
Vol 38 (1) ◽  
pp. 198-209 ◽  
Author(s):  
R. W. Knighton

1. To show conclusively that the electrically evoked retinal response (EERG) is in fact an electrically evoked component of the usual ERG, it is necessary to show that the two responses have the same intraretinal pattern of current generators. A method for determining when two responses have the same origin has been developed. This method utilizes measurements from a single microelectrode penetrating the retina. 2. The method was sensitive enough to detect differences in the origins of two responses when they were present. 3. The EERG had the same intraretinal origin as the PII component of the ERG, and thus is the PII component evoked by electricity rather than by light. 4. The hypothesis that electrical stimuli act on the synaptic terminals of the photoreceptor cells predicts that electrical current will evoke components of the ERG. The fact that the EERG is an electrically evoked component of the ERG fulfills that prediction.

1975 ◽  
Vol 38 (1) ◽  
pp. 185-197 ◽  
Author(s):  
R. W. Knighton

1. Brief pulses of electrical current passed through the frog's eye cup from sclera to vitreous (inward current) evoked large, vitreous positive slow potentials of retinal origin. Brief pulses of outward current evoke no response. 2. This electrically evoked retinal response (EERG) increased sharply in amplitude as the strength of the stimulus was increased. 3. Strength-duration curves showed that the stimulating pulse was integrated with a time constant ranging from 14 to 36 ms. The time constant was a function of the amplitude chosen as a criterion. 4. The EERG was abolished by chemical agents which abolish the PII component of the ERG (KCl, NH3, aspartate). 5. The waveform of the EERG returned to the base line with exactly the same time course as the PII component of the ERG, both when the PII was evoked by flashes of light and when it was evoked by flashes of darkness. 6. These results can be explained in terms of the hypothesis that electrical stimuli act on the synaptic terminals of the photoreceptor cells.


1969 ◽  
Vol 54 (3) ◽  
pp. 310-330 ◽  
Author(s):  
Ronald Millecchia ◽  
Alexander Mauro

The ventral photoreceptors of Limulus polyphemus are unipolar cells with large, ellipsoidal somas located long both "lateral olfactory nerves." As a consequence of their size and location, the cells are easily impaled with microelectrodes. The cells have an average resting potential of -48 mv. The resting potential is a function of the external concentration of K. When the cell is illuminated, it gives rise to the typical "receptor potential" seen in most invertebrate photoreceptors which consists of a transient phase followed by a maintained phase of depolarization. The amplitude of the transient phase depends on both the state of adaptation of the cell and the intensity of the illumination, while the amplitude of the maintained phase depends only on the intensity of the illumination. The over-all size of the receptor potential depends on the external concentration of Na, e.g. in sodium-free seawater the receptor potential is markedly reduced, but not abolished. On the other hand lowering the Ca concentration produces a marked enhancement of both components of the response, but predominantly of the steady-state component. Slow potential fluctuations are seen in the dark-adapted cell when it is illuminated with a low intensity light. A spike-like regenerative process can be evoked by either the receptor potential or a current applied via a microelectrode. No evidence of impulse activity has been found in the axons of these cells. The ventral photoreceptor cell has many properties in common with a variety of retinular cells and therefore should serve as a convenient model of the primary receptor cell in many invertebrate eyes.


In a recent communication to the Society I have stated that the normal electrical response of the eye to light consists in a current traversing the eye ball in a positive direction, i. e . from fundus to cornea, and that a similar positive response is aroused by meachanical and by electrical stimuli, whatever be the direction of the latter. I have designated this positive response to non-luminous stimuli as the retainal discharge or blaze. With reference to electrical stimulation, its most characteristic from is when it occurs in the same dorection as the current by which it is excited, i. e . when it cannot be polarisation counter-current. It is in such case analogous with the discharge of an electrical organ excited by an electrical current in the direction of normal discharge, and, indeed, it may be of such magnitued as to lead an observer to regard retain in the light of an electrical organ.


Author(s):  
W. Krebs ◽  
I. Krebs

Various inclusion bodies occur in vertebrate retinal photoreceptor cells. Most of them are membrane bound and associated with phagocytosis or they are age related residual bodies. We found an additional inclusion body in foveal cone cells of the baboon (Papio anubis) retina.The eyes of a 15 year old baboon were fixed by immersion in cacodylate buffered glutaraldehyde (2%)/formaldehyde (2%) as described in detail elsewhere . Pieces of retina from various locations, including the fovea, were embedded in epoxy resin such that radial or tangential sections could be cut.Spindle shaped inclusion bodies were found in the cytoplasm of only foveal cones. They were abundant in the inner segments, close to the external limiting membrane (Fig. 1). But they also occurred in the outer fibers, the perikarya, and the inner fibers (Henle’s fibers) of the cone cells. The bodies were between 0.5 and 2 μm long. Their central diameter was 0.2 to 0. 3 μm. They always were oriented parallel to the long axis of the cone cells. In longitudinal sections (Figs. 2,3) they seemed to have a fibrous skeleton that, in cross sections, turned out to consist of plate-like (Fig.4) and tubular profiles (Fig. 5).


Author(s):  
Maria Anna Pabst

In addition to the compound eyes, honeybees have three dorsal ocelli on the vertex of the head. Each ocellus has about 800 elongated photoreceptor cells. They are paired and the distal segment of each pair bears densely packed microvilli forming together a platelike fused rhabdom. Beneath a common cuticular lens a single layer of corneagenous cells is present.Ultrastructural studies were made of the retina of praepupae, different pupal stages and adult worker bees by thin sections and freeze-etch preparations. In praepupae the ocellar anlage consists of a conical group of epidermal cells that differentiate to photoreceptor cells, glial cells and corneagenous cells. Some photoreceptor cells are already paired and show disarrayed microvilli with circularly ordered filaments inside. In ocelli of 2-day-old pupae, when a retinogenous and a lentinogenous cell layer can be clearly distinguished, cell membranes of the distal part of two photoreceptor cells begin to interdigitate with each other and so start to form the definitive microvilli. At the beginning the microvilli often occupy the whole width of the developing rhabdom (Fig. 1).


Author(s):  
Matti Järvilehto ◽  
Riitta Harjula

The photoreceptor cells in the compound eyes of higher diptera are clustered in groups (ommatidia) of eight receptor cells. The cells from six adjacent ommatidia are organized into optical units, neuro-ommatia sharing the same visual field. In those ommatidia the optical axes of the photopigment containing structures (rhabdomeres) are parallel. The rhabdomeres of the photoreceptor cells are separated from each other by an interstitial i.e innerommatidial space (IOS). In the photoreceptor cell body, besides of the normal cell organelles, a cellular matrix is a structurally apparent component. Similar kind of reticular formation is also found in the IOS containing some unidentified filamentary substance, of which composition and functional significance for optical properties of vision is the aim of this report.The prefixed (2% PA + 0.2% GA in 0.1-n phosphate buffer, pH 7.4, for 1h), frozen section blocks of the compound eye of the blowfly (Calliphora erythrocephala) were prepared by immuno-cryo-techniques. The ultrathin cryosections were incubated with antibodies of monoclonal α-tubulin and polyclonal smooth muscle actin. Control labelings of excess of antigen, non-immune serum and non-present antibody were perforated.


1990 ◽  
Vol 29 (04) ◽  
pp. 282-288 ◽  
Author(s):  
A. van Oosterom

AbstractThis paper introduces some levels at which the computer has been incorporated in the research into the basis of electrocardiography. The emphasis lies on the modeling of the heart as an electrical current generator and of the properties of the body as a volume conductor, both playing a major role in the shaping of the electrocardiographic waveforms recorded at the body surface. It is claimed that the Forward-Problem of electrocardiography is no longer a problem. Several source models of cardiac electrical activity are considered, one of which can be directly interpreted in terms of the underlying electrophysiology (the depolarization sequence of the ventricles). The importance of using tailored rather than textbook geometry in inverse procedures is stressed.


2020 ◽  
Vol 25 (3) ◽  
pp. 415-423
Author(s):  
Ahmed Lachhab ◽  
El Mehdi Benyassine ◽  
Mohamed Rouai ◽  
Abdelilah Dekayir ◽  
Jean C. Parisot ◽  
...  

The tailings of Zeida's abandoned mine are found near the city of Midelt, in the middle of the high Moulouya watershed between the Middle and the High Atlas of Morocco. The tailings occupy an area of about 100 ha and are stored either in large mining pit lakes with clay-marl substratum or directly on a heavily fractured granite bedrock. The high contents of lead and arsenic in these tailings have transformed them into sources of pollution that disperse by wind, runoff, and seepage to the aquifer through faults and fractures. In this work, the main goal is to identify the pathways of contaminated water with heavy metals and arsenic to the local aquifers, water ponds, and Moulouya River. For this reason, geophysical surveys including electrical resistivity tomography (ERT), seismic refraction tomography (SRT) and very low-frequency electromagnetic (VLF-EM) methods were carried out over the tailings, and directly on the substratum outside the tailings. The result obtained from combining these methods has shown that pollutants were funneled through fractures, faults, and subsurface paleochannels and contaminated the hydrological system connecting groundwater, ponds, and the river. The ERT profiles have successfully shown the location of fractures, some of which extend throughout the upper formation to depths reaching the granite. The ERT was not successful in identifying fractures directly beneath the tailings due to their low resistivity which inhibits electrical current from propagating deeper. The seismic refraction surveys have provided valuable details on the local geology, and clearly identified the thickness of the tailings and explicitly marked the boundary between the Triassic formation and the granite. It also aided in the identification of paleochannels. The tailings materials were easily identified by both their low resistivity and low P-wave velocity values. Also, both resistivity and seismic velocity values rapidly increased beneath the tailings due to the compaction of the material and lack of moisture and have proven to be effective in identifying the upper limit of the granite. Faults were found to lie along the bottom of paleochannels, which suggest that the locations of these channels were caused by these same faults. The VLF-EM surveys have shown tilt angle anomalies over fractured areas which were also evinced by low resistivity area in ERT profiles. Finally, this study showed that the three geophysical methods were complementary and in good agreement in revealing the pathways of contamination from the tailings to the local aquifer, nearby ponds and Moulouya River.


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