scholarly journals Cerebrospinal Fluid Secretion by the Choroid Plexus

2013 ◽  
Vol 93 (4) ◽  
pp. 1847-1892 ◽  
Author(s):  
Helle H. Damkier ◽  
Peter D. Brown ◽  
Jeppe Praetorius
Keyword(s):  
Cerebrospinal Fluid ◽  
Choroid Plexus ◽  
Epithelial Transport ◽  
Cell Monolayer ◽  
Fluid Secretion ◽  
Transport Proteins ◽  
High Rate ◽  
Electrolyte Balance ◽  
Cuboidal Cell ◽  
Cerebrospinal Fluid Secretion

The choroid plexus epithelium is a cuboidal cell monolayer, which produces the majority of the cerebrospinal fluid. The concerted action of a variety of integral membrane proteins mediates the transepithelial movement of solutes and water across the epithelium. Secretion by the choroid plexus is characterized by an extremely high rate and by the unusual cellular polarization of well-known epithelial transport proteins. This review focuses on the specific ion and water transport by the choroid plexus cells, and then attempts to integrate the action of specific transport proteins to formulate a model of cerebrospinal fluid secretion. Significant emphasis is placed on the concept of isotonic fluid transport across epithelia, as there is still surprisingly little consensus on the basic biophysics of this phenomenon. The role of the choroid plexus in the regulation of fluid and electrolyte balance in the central nervous system is discussed, and choroid plexus dysfunctions are described in a very diverse set of clinical conditions such as aging, Alzheimer's disease, brain edema, neoplasms, and hydrocephalus. Although the choroid plexus may only have an indirect influence on the pathogenesis of these conditions, the ability to modify epithelial function may be an important component of future therapies.

scholarly journals Cerebrospinal fluid secretion by the choroid plexus?

2016 ◽  
Vol 96 (4) ◽  
pp. 1661-1662 ◽  
Author(s):  
Darko Orešković ◽  
Milan Radoš ◽  
Marijan Klarica
Keyword(s):  
Cerebrospinal Fluid ◽  
Choroid Plexus ◽  
Fluid Secretion ◽  
Cerebrospinal Fluid Secretion

scholarly journals Genetic disruption of slc4a10 alters the capacity for cellular metabolism and vectorial ion transport in the choroid plexus epithelium

2020 ◽  
Vol 17 (1) ◽  
Author(s):  
Inga Baasch Christensen ◽  
Qi Wu ◽  
Anders Solitander Bohlbro ◽  
Marianne Gerberg Skals ◽  
Helle Hasager Damkier ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Epithelial Cells ◽  
Choroid Plexus ◽  
Genetic Manipulation ◽  
Fluid Secretion ◽  
Transport Proteins ◽  
High Rate ◽  
Mitochondrial Enzymes ◽  
Tandem Mass Tag ◽  
Quantitative Immunoblotting

Abstract Background Genetic disruption of slc4a10, which encodes the sodium-dependent chloride/bicarbonate exchanger Ncbe, leads to a major decrease in Na+-dependent HCO3− import into choroid plexus epithelial cells in mice and to a marked reduction in brain intraventricular fluid volume. This suggests that Ncbe functionally is a key element in vectorial Na+ transport and thereby for cerebrospinal fluid secretion in the choroid plexus. However, slc4a10 disruption results in severe changes in expression of Na+,K+-ATPase complexes and other major transport proteins, indicating that profound cellular changes accompany the genetic manipulation. Methods A tandem mass tag labeling strategy was chosen for quantitative mass spectrometry. Alterations in the broader patterns of protein expression in the choroid plexus in response to genetic disruption of Ncbe was validated by semi-quantitative immunoblotting, immunohistochemistry and morphometry. Results The abundance of 601 proteins were found significantly altered in the choroid plexus from Ncbe ko mice relative to Ncbe wt. In addition to a variety of transport proteins, particularly large changes in the abundance of proteins involved in cellular energy metabolism were detected in the Ncbe ko mice. In general, the abundance of rate limiting glycolytic enzymes and several mitochondrial enzymes were reduced following slc4a10 disruption. Surprisingly, this was accompanied by increased ATP levels in choroid plexus cells, indicating that the reduction in capacity for energy metabolism was adaptive to high ATP rather than causal for a decreased capacity for ion and water transport. Ncbe-deficient cells also had a reduced cell area and decreased K+ content. Conclusion Our findings suggest that the lack of effective Na+-entry into the epithelial cells of the choroid plexus leads to a profound change in the cellular phenotype, shifting from a high-rate secretory function towards a more dormant state; similar to what is observed during ageing or Alzheimer’s disease.

Opinion of the Pathogenesis of the Mumps Meningites

2020 ◽  
Keyword(s):  
Cerebrospinal Fluid ◽  
Intracranial Pressure ◽  
Choroid Plexus ◽  
Present Article ◽  
Clinical Data ◽  
Fluid Secretion ◽  
Mumps Virus ◽  
Direct Involvement ◽  
Glymphatic System ◽  
Cerebrospinal Fluid Secretion

It is generally accepted that meningeal reactions in patients with mumps are due to the direct involvement of the meninges by the mumps virus. With the development of mumps vaccines, this view was extended to vaccinated people, who are considered serious post-vaccine meningitis. In present article, the author states that these reactions are not due to inflammation of the meninges, but to the choroid plexus caused by virulent and vaccine strains. Inflammation leads to an increase in cerebrospinal fluid secretion, which increases intracranial pressure and is manifested by meningeal symptoms. In the presence of this evidence, the author considers that meningeal reactions are not meningitis, but meningisms, based on clinical data, experiments on monkeys and the glymphatic system.

Choroid Plexus Ion Transporter Expression and Cerebrospinal Fluid Secretion

Brain Edema X ◽  
1997 ◽  
pp. 279-281
Author(s):  
Richard F. Keep ◽  
J. Xiang ◽  
L. J. Ulanski ◽  
F. C. Brosius ◽  
A. Lorris Betz
Keyword(s):  
Cerebrospinal Fluid ◽  
Choroid Plexus ◽  
Fluid Secretion ◽  
Ion Transporter ◽  
Transporter Expression ◽  
Cerebrospinal Fluid Secretion

The GLP-1R agonist exendin-4 reduces cerebrospinal fluid secretion and intracranial pressure

2017 ◽  
Author(s):  
Hannah Botfield ◽  
Maria Uldall ◽  
Connar Westgate ◽  
James Mitchell ◽  
Snorre Hagen ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Intracranial Pressure ◽  
Fluid Secretion ◽  
Cerebrospinal Fluid Secretion

History, Anatomy, Histology, and Embryology of the Ventricles and Physiology of the Cerebrospinal Fluid

2021 ◽  
Author(s):  
Pinar Kuru Bektaşoğlu ◽  
Bora Gürer
Keyword(s):  
Cerebrospinal Fluid ◽  
Fluid Secretion ◽  
Colorless Liquid ◽  
Brain Ventricles ◽  
Arachnoid Granulations ◽  
Choroid Plexuses ◽  
Subarachnoid Spaces ◽  
The Mean ◽  
The Brain ◽  
Cerebrospinal Fluid Secretion

Cerebrospinal fluid is an essential, clear, and colorless liquid for the homeostasis of the brain and neuronal functioning. It circulates in the brain ventricles, the cranial and spinal subarachnoid spaces. The mean cerebrospinal fluid volume is 150 ml, with 125 ml in subarachnoid spaces and 25 ml in the ventricles. Cerebrospinal fluid is mainly secreted by the choroid plexuses. Cerebrospinal fluid secretion in adults ranges between 400 and 600 ml per day and it is renewed about four or five times a day. Cerebrospinal fluid is mainly reabsorbed from arachnoid granulations. Any disruption in this well-regulated system from overproduction to decreased absorption or obstruction could lead to hydrocephalus.

scholarly journals Immunocytochemical localization of Na+,K+-ATPase catalytic polypeptide in mouse choroid plexus.

1986 ◽  
Vol 34 (2) ◽  
pp. 189-195 ◽  
Author(s):  
S A Ernst ◽  
J R Palacios ◽  
G J Siegel
Keyword(s):  
Choroid Plexus ◽  
Epithelial Transport ◽  
Protein A ◽  
Fluid Secretion ◽  
Physiological Data ◽  
Electron Microscopic ◽  
Antibody Technique ◽  
Immunogold Staining ◽  
Thin Sections ◽  
Membrane Surfaces

Na+,K+-ATPase plays a central role in the mechanism of cerebrospinal fluid secretion by the choroid plexus. We have used an antiserum to the 100 KD catalytic polypeptide of the enzyme purified from mouse brain (30) to localize the catalytic unit in mouse choroid plexus at the light and electron microscopic levels. Pre-embedding immunostaining with the peroxidase-conjugated second antibody technique showed that microvillar borders facing the ventricle were intensely reactive. In contrast, basal and lateral plasma membrane surfaces were devoid of activity. Identical localization was obtained with a post-embedding procedure in which protein A-gold was used to stain immunoreactive sites on thin sections of Lowicryl-embedded tissue. For comparison, immunogold staining was shown to be restricted to basolateral membranes of kidney medullary ascending thick limbs. The apical localization of Na+,K+-ATPase in choroid plexus is in striking contrast to the almost exclusive basolateral localization seen in other ion-transporting tissues. The immunocytochemical data are completely consistent with physiological data on choroidal epithelial transport and with light microscopic autoradiographic localization of [3H]-ouabain binding sites.

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