Mn2+–DNA interactions in aqueous systems: A Raman spectroscopic study
2006 ◽
Vol 20
(1)
◽
pp. 29-35
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Keyword(s):
Interaction of natural calf thymus DNA with Mn2+ions was studied by means of Raman spectroscopy. Spectra of DNA in 10 mM Na-cacodylate buffer, pH 6.2, 10 mM NaCl and in buffer containing Mn2+ions were measured at room temperature. Mn2+concentrations varied between 0 and 0.6 M. DNA backbone conformational changes and DNA denaturation were not observed in the concentration range 0 and 0.5 M, however, DNA condensation was observed at a critical concentration of 100 mM Mn2+that prevented the measurement of Raman spectra. Binding of Mn2+to the charged phosphate groups of DNA is indicated in the spectra. A high affinity of Mn2+for guanine N7 was obvious, and binding to adenine was barely suggested.