scholarly journals Analysis of Transcripts Expressed in One-Day-Old Larvae and Fifth Instar Silk Glands of Tasar Silkworm,Antheraea mylitta

2010 ◽  
Vol 2010 ◽  
pp. 1-11 ◽  
Author(s):  
Samita Maity ◽  
Sagar I. Goel ◽  
Sobhan Roy ◽  
Suvankar Ghorai ◽  
Swati Bhattacharyya ◽  
...  

Antheraea mylittais one of the wild nonmulberry silkworms, which produces tasar silk. An EST project has been undertaken to understand the gene expression profile ofA. mylittasilk gland. Two cDNA libraries, one from the whole bodies of one-day-old larvae and the other from the silkglands of fifth instar larvae, were constructed and sequenced. A total of 2476 good-quality ESTs (1239 clones) were obtained and grouped into 648 clusters containing 390 contigs and 258 singletons to represent 467 potential unigenes. Forty-five sequences contained putative coding region, and represented potentially novel genes. Among the 648 clusters, 241 were categorized according to Gene Ontology hierarchy and showed presence of several silk and immune-related genes. TheA. mylittaESTs have been organized into a freely available online database “AmyBASE”. These data provide an initial insight into theA. mylittatranscriptome and help to understand the molecular mechanism of silk protein production in a Lepidopteran species.

2011 ◽  
Vol 7 (1) ◽  
pp. 80-84 ◽  
Author(s):  
G. Lokesh ◽  
Putkho Paul Pao ◽  
K.N. Madhusudha ◽  
P.K. Kar ◽  
A.K. Srivastava ◽  
...  

1969 ◽  
Vol 42 (1) ◽  
pp. 284-295 ◽  
Author(s):  
Allen L. Bell ◽  
David B. Peakall

The ampullate silk gland of the spider, Araneus sericatus, produces the silk fiber for the scaffolding of the web. The fine structure of the various parts of the gland is described. The distal portion of the duct consist of a tube of epithelial cells which appear to secrete a substance which forms the tunica intima of the duct wall. At the proximal end of the duct there is a region of secretory cells. The epithelium of the sac portion contains five morphologically distinct types of granules. The bulk of the synthesis of silk occurs in the tail of the gland, and in this region only a single type of secretory droplet is seen in the epithelium. Protein synthesis can be stimulated by the injection of 1 mg/kg acetylcholine into the body fluids. 10 min after injection, much of the protein stored in the cytoplasm of the epithelial cells has been secreted into the lumen. 20 min after stimulation, the ergastoplasmic sacs form large whorls in the cytoplasm. Protein, similar in electron-opacity to protein found in the lumen, begins to form in that portion of the cytoplasm which is enclosed by the whorls. The limiting membrane of these droplets is formed by ergastoplasmic membranes which lose their ribosomes. No Golgi material has been found in these cells. Protein appears to be manufactured in the cytoplasm of the tail cells in a form which is ready for secretion.


Gene ◽  
2006 ◽  
Vol 370 ◽  
pp. 51-57 ◽  
Author(s):  
Botlagunta Mahendran ◽  
Chitrangada Acharya ◽  
Rupesh Dash ◽  
Sudip K. Ghosh ◽  
S.C. Kundu

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