scholarly journals Performance of a Whole-Blood Interferon-Gamma Release Assay withMycobacteriumRD1-Specific Antigens among HIV-Infected Persons

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Akira Fujita ◽  
Atsushi Ajisawa ◽  
Nobuyuki Harada ◽  
Kazue Higuchi ◽  
Toru Mori
Keyword(s):  
Interferon Gamma ◽  
High Sensitivity ◽  
Positive Control ◽  
Interferon Gamma Release Assay ◽  
Cell Counts ◽  
Positive Rate ◽  
Release Assay ◽  
Specific Antigens ◽  
Proportional Relationship ◽  
Interferon Gamma Response

Objective. To evaluate the usefulness of one of IGRAs, QuantiFERON-TB Gold (QFT-G), in human immunodeficiency virus- (HIV- ) infected patients with variousCD4+T cell counts.Methods. The QFT-G assay was performed using QFT-G kits among 107 HIV-infected patients including 9 cases with active tuberculosis (TB).Results. In HIV-infected patients withCD4+>50/μL, QFT-G positive rate for active TB patients was 5/6 (sensitivity=83%), and that for those without active disease was 1/69 (specificity=99%). The frequency of indeterminate QFT-G test was significantly higher in those withCD4+less than50/μL(P<.0001). At the same time there was a proportional relationship betweenCD4+and interferon-gamma response to mitogen (positive control) in QFT-G test (P=.0001).Conclusions. Our data suggested that QFT-G had high sensitivity and specificity in HIV-infected populations withCD4+greater than50/μL. However, QFT-G did not perform well in HIV-positive patients withCD4+less than50/μL.

An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study

2018 ◽  
Vol 57 (6) ◽  
pp. 724-732 ◽  
Author(s):  
Marcela Rubio-Carrasquilla ◽  
Cristian David Santa ◽  
Juan Pablo Rendón ◽  
Jorge Botero-Garcés ◽  
Allan J Guimarães ◽  
...  
Keyword(s):  
Risk Factors ◽  
Interferon Gamma ◽  
Histoplasma Capsulatum ◽  
Screening Method ◽  
High Sensitivity ◽  
Positive Control ◽  
Interferon Gamma Release Assay ◽  
Predictive Values ◽  
Release Assay ◽  
Ifn Γ

AbstractHistoplasmosis is the most common endemic mycosis in the Americas. Currently, there is no laboratory test capable to detect subclinical or latent infections by Histoplasma capsulatum (Hc), which might develop as severe infections in immunocompromised individuals. For the first time to our knowledge, we explore the suitability of an interferon gamma release assay (IGRA) to detect latent Hc infection in asymptomatic individuals. A cohort of 126 volunteers was enrolled in the study, 13 of which underwent a Hc infection in the past, and 93 of them showing risk factors for this infection. The remaining 20 participants did not refer any risk factors of Hc infection, but eight of them showed evidences of infection with Mycobacterium tuberculosis. All participants were recruited in Medellin, Colombia, between January 2014 and December 2017. Whole blood samples were cultured with four different Hc crude antigens and phytohemaglutinin as positive control. The interferon (IFN)-γ released by T lymphocytes upon antigen stimulation was quantified by ELISA. A defined cutoff value of 20 pg/ml for the IFN-γ concentration allowed us to distinguish between the group with documented past infections and the group of noninfected individuals with high sensitivity (70–92%) and specificity (85–95%), for the four tested antigens. Positive 82–95% and negative 77–92% predictive values were also very high, comparable to those reported for commercially available IGRAs. The new test constitutes a promising screening method to detect individuals with latent Hc infection, even decades after the primary infection, as evidenced in this study.

scholarly journals CORRELATION BETWEEN INTERFERON GAMMA RELEASE ASSAY OF ELISPOT METHOD AND CD4+ T LYMPHOCYTE CELL COUNT IN HIV POSITIVE PATIENTS

2019 ◽  
Vol 25 (3) ◽  
pp. 333
Author(s):  
Nabil Salim Ambar ◽  
Aryati Aryati ◽  
Tutik Kusmiati ◽  
Erwin Astha Triyono
Keyword(s):  
Cell Count ◽  
Interferon Gamma ◽  
T Lymphocyte ◽  
Hiv Positive ◽  
Interferon Gamma Release Assay ◽  
Spearman Correlation ◽  
Cell Counts ◽  
The World ◽  
Release Assay ◽  
Lymphocyte Cell

Introduction. HIV is a virus that can cause AIDS, which affects the immune system and weakens the body function in fighting disease. The primary cells that HIV attacks are CD4+ T lymphocytes. Opportunistic Infections (OIs) are the biggest risk factors of death in HIV patients and occur in CD4+ T cells <200 cells/μL lymphocytes. TB is a disease with a high mortality rate in the world where Indonesia is a TB endemic country with the highest morbidity rates of TB in the world. The most common OI in people with HIV is TB. The number of limitations on Tuberculin Skin Test (TST) is large, thus in vitro T cells test with (Interferon Gamma Release Assay) IGRA is used in diagnosing latent TB. The aim of this study was to determine the correlation between IGRA ELISPOT method and CD4+ T lymphocyte cell count in HIV positive patients.Method. This was an observational analytical study with cross sectional design. The number of samples was 56 HIV positive patients who were treated at the UPIPI Clinic of the Dr Soetomo Surabaya Hospital. The examination of CD4+ T lymphocyte count was perfomed with FACSCalibur and IGRA was examined with T-SPOT.TB. The results were analyzed using Spearman correlation test.Results. CD4 + lymphocyte cell counts based on WHO groupings were as follows: > 500 cells / μL (33.92%), 200-349 cells / μL (25%), 350-499 cells / μL (25%) and <200 cells / μL (16 , 07%). IGRA examination results showed 35.18% positive and 64.81% negative. The grouping of CD4+ T lymphocyte cell counts based on IGRA test results was 27.77% with positive IGRA and 48.14% with negative IGRA. Spearman correlation test between CD4+ T cell lymphocytes with IGRA in HIV positive patients showed r = 0,036 (p = 0,794).Conclusion. There was no correlation between interferon gamma release assay of ELISPOT method and CD4+ T lymphocyte cell count in HIV positive patients.

scholarly journals Performance of an interferon-gamma release assay for diagnosing latent tuberculosis infection in children

2007 ◽  
Vol 136 (9) ◽  
pp. 1179-1187 ◽  
Author(s):  
K. OKADA ◽  
T. E. MAO ◽  
T. MORI ◽  
T. MIURA ◽  
T. SUGIYAMA ◽  
...  
Keyword(s):  
Interferon Gamma ◽  
Latent Tuberculosis ◽  
Mycobacterial Infection ◽  
Sputum Smear ◽  
Interferon Gamma Release Assay ◽  
Test Results ◽  
Positive Rate ◽  
Release Assay ◽  
Latent Tb ◽  
Latent Tb Infection

SUMMARYNewly developed interferon-gamma release assays have become commercially available to detect tuberculosis (TB) infection in adults. However, little is known about their performance in children. We compared test results between the QuantiFERON-TB® Gold test (QFT) and tuberculin skin test (TST) in young children living with pulmonary TB patients in Cambodia. Of 195 children tested with both QFT and TST, the TST-positive rate of 24% was significantly higher than the QFT-positive rate of 17%. The agreement between the test results was considerable (κ-coefficient 0·63). Positive rates increased from 6% to 32% for QFT and from 15% to 43% for TST, according to the sputum smear grades of the index cases. The presence of Bacille Calmette-Guérin (BCG) scars did not significantly affect the results of TST or QFT in a logistic regression analysis. In conclusion, QFT can be a substitute for TST in detecting latent TB infection in childhood contacts aged ⩽5 years, especially in those who may have a false-positive TST due to BCG vaccination or non-tuberculous mycobacterial infection.

scholarly journals Role of interferon gamma release assay in the diagnosis and management of Mycobacterium tuberculosis-associated uveitis: a review

2021 ◽  
Vol 6 (1) ◽  
pp. e000663
Author(s):  
Samra Rahman ◽  
Muhammad Irfan ◽  
M A Rehman Siddiqui
Keyword(s):  
Interferon Gamma ◽  
Interferon Gamma Release Assay ◽  
Limited Evidence ◽  
Ocular Tuberculosis ◽  
Diagnosis And Management ◽  
Pubmed Database ◽  
Interferon Gamma Release Assays ◽  
Infectious Uveitis ◽  
Release Assay

Tuberculosis (TB)-associated uveitis is a common cause of infectious uveitis in the developing world. Diagnosis of TB uveitis remains a challenge. The role of interferon gamma release assays (IGRAs) is uncertain. Herein we summarise the available literature on the utility of IGRAs in the diagnosis and management of TB uveitis. We searched PubMed database from 1 August 2010 to 31 July 2020 using the following keywords alone and in combination: ‘interferon-gamma release assay’, ‘QuantiFERON’, ‘T-SPOT.TB’, ‘TB uveitis’, ‘serpiginous like choroiditis’, ‘tuberculoma’, ‘TB vasculitis’, ‘TB panuveitis’ and ‘ocular tuberculosis’. Data from 58 relevant studies were collated. The review is focused on currently marketed versions of IGRA tests: QuantiFERON-TB Gold In-Tube assay, QuantiFERON-TB Gold Plus assay (QFT-Plus) and T-SPOT.TB. We found limited evidence regarding the diagnostic utility of IGRA in patients with uveitis. No study was identified evaluating the newer QFT test—the QFT-Plus—in patients with uveitis. Similarly, there is lack of data directly comparing QFT-Plus with T-SPOT.TB specifically for the diagnosis of TB uveitis.

Esophageal Tuberculosis in a Patient on Maintenance Dialysis: Advantages of Interferon-Gamma Release Assay

Renal Failure ◽  
2009 ◽  
Vol 31 (3) ◽  
pp. 248-250 ◽  
Author(s):  
Olesia Damaschin ◽  
Omar Dahmani ◽  
Frédéric Faibis ◽  
Marie-Claude Demachy ◽  
Mahdi Abtahi ◽  
...  
Keyword(s):  
Interferon Gamma ◽  
Interferon Gamma Release Assay ◽  
Esophageal Tuberculosis ◽  
Release Assay ◽  
Maintenance Dialysis
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