scholarly journals High Performance Thin Layer Chromatographic Determination of Chrysin inOroxylum IndicumVent. from Different Geographical Regions of India

2012 ◽  
Vol 9 (1) ◽  
pp. 313-317 ◽  
Author(s):  
K. Srilatha Srinivas ◽  
A. Saraf Aparna
Keyword(s):  
Western Ghats ◽  
High Performance ◽  
Raw Materials ◽  
Uttar Pradesh ◽  
Chromatographic Determination ◽  
Solvent System ◽  
Chemical Markers ◽  
Geographical Regions ◽  
Maximum Content

HPTLC fingerprints can be used for documentation and quantification of chemical markers to identify morphological and geographical variation in herbal raw materials. The current work was aimed at developing phytochemical fingerprints ofOroxylum indicumvent. from different geographical regions of Indiaviz. Western Ghats (Maharashtra) and Northern Uttar Pradesh. Fingerprint of flavonoid, chrysin was developed for roots, leaves and bark of the plant from different regions using cold extraction and suitable solvent system. HPTLC technique was successfully used for evaluating regional variations. The fingerprints were also utilized for quantification of the flavonoid, chrysin from different parts of the plants from two distinct geographical regions. Maximum content of chrysin was found in plant collected from Western Ghats than in U.P.

High Performance Liquid Chromatographic Determination of Bifidobacterium bifidum Growth Factors in Human Milk

1983 ◽  
Vol 66 (1) ◽  
pp. 135-139
Author(s):  
Samy H Ashoor ◽  
Woodrow C Monte
Keyword(s):  
Growth Factors ◽  
Human Milk ◽  
High Performance ◽  
Chromatographic Determination ◽  
Solvent System ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Bifidobacterium Bifidum ◽  
Liquid Chromatographic

Abstract An isocratic high performance liquid chromatography (HPLC) method has been developed for the determination of Bifidobacterium bifidum growth factors in human milk. The method involves the gradual addition of 3 volumes of ethanol to the milk sample, filtration, and analysis of the growth factors in the filtrate by HPLC. The HPLC system consisted of a carbohydrate analysis column, a water-acetonitrile (70 + 30) solvent system, a flow rate of 1.0 mL/min, and a refractive index detector. The method is simpler and requires less time than the present microbiological method. Moreover, it revealed for the first time the presence of 2 separable growth factors in all human milk samples tested. The HPLC method developed is sensitive and can be used to monitor the type and the amount of growth factors in mothers’ milk during lactation.

High Performance Liquid Chromatographic Determination of Vitamin D in Fortified Milks, Margarine, and Infant Formulas

1982 ◽  
Vol 65 (3) ◽  
pp. 624-631 ◽  
Author(s):  
James N Thompson ◽  
George Hatina ◽  
William B Maxwell ◽  
Suzanne Duval
Keyword(s):  
Vitamin D ◽  
High Performance ◽  
Room Temperature ◽  
Chromatographic Determination ◽  
Solvent System ◽  
High Performance Liquid Chromatographic ◽  
Coefficients Of Variation ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract Fortified milks were saponified overnight at room temperature with 1% ethanolic pyrogallol and KOH. The digest was extracted with hexane after adding water and ethanol, and the extract was washed consecutively with 5% KOH, water, and 55% aqueous ethanol to remove polar lipids. After evaporation, the residue was first chromatographed on a column of 5 μm silica. A fraction containing vitamin D was collected, evaporated, and rechromatographed on a reverse phase column for the separation and quantitation of vitamins D2 and D3. Recovery was 96-99% and the coefficient of variation was 3% (8 replicates). Infant formula was diluted and then saponified and extracted as in the analysis of milk. Margarine was saponified by shaking overnight with 1% ethanolic pyrogallol and 80% KOH. Water and ethanol were added to the digest before extraction. Extracts from formula and margarine were chromatographed as milk except, before HPLC, the extract was dissolved in isopropanol-hexane (1 + 99) and passed through 5 cm alumina in a Pasteur pipet, and the concentration of isopropanol in the first high performance liquid chromatographic (HPLC) solvent system was halved to improve the separation of vitamin D from other absorbing lipids. Usually several peaks were obtained during the final HPLC analysis, and the identification of vitamins D2 and D3 was less certain than in the analysis of milk. The coefficients of variation for formula and margarine were 6% (5 replicates) and 9% (6 replicates), respectively.

High Performance Liquid Chromatographic Determination of Subsidiary Colors in FD&C Red No. 3

1982 ◽  
Vol 65 (5) ◽  
pp. 1080-1085
Author(s):  
Robert J Calvey ◽  
Allen L Goldberg
Keyword(s):  
High Performance ◽  
Chromatographic Determination ◽  
Total Sample ◽  
Solvent System ◽  
High Performance Liquid Chromatographic ◽  
Phase Method ◽  
Reverse Phase ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract Two rapid, sensitive, reproducible methods that use a Zorbax C-8 reverse phase column and high performance liquid chromatography are described for the determination of the subsidiary colors in FD&C Red No. 3. With the first method, 8 subsidiary colors (fluorescein, 2'-iodofluorescein, 4'-iodofluorescein, 2',5'-diiodofluorescein, 2',7'-diiodofluorescein, 4',- 5'-diiodofluorescein, 2',4',7'-triiodofluorescein, and 2',4',5'-triiodofluorescein) are eluted in a reproducible pattern by increasing the organic nature of a buffered mobile phase. Method 1 is capable of quantitating all the subsidiary colors except 4'-iodofluorescein and 4',5'-diiodofluorescein. If these 2 subsidiary colors are seen, the sample must be run again by method 2, which uses a different program and solvent system to quantitate them. The average recoveries for the 6 subsidiary colors quantitatively determined in FD&C Red No. 3 by method 1 ranged from 96 to 98%. The average recoveries for 4'-iodofluorescein and 4',5'-diiodofluorescein, quantitatively determined in FD&C Red No. 3 by method 2, were 101 and 103%, respectively. The amounts of the 6 subsidiary colors recovered by method 1 were 0.04-7.4% by weight of the total sample. The amounts of the 2 subsidiary colors recovered by method 2 were 0.13-2.6% by weight of the total sample.

High-performance thin-layer chromatographic determination of rosiglitazone in its dosage form

2002 ◽  
Vol 15 (3) ◽  
pp. 192-195 ◽  
Author(s):  
Ramesh Sane ◽  
Mary Francis ◽  
Atul Moghe ◽  
Sachin Khedkar ◽  
Ajit Anerao
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Dosage Form ◽  
Chromatographic Determination

Liquid Chromatographic Determination of Amiodarone Hydrochloride and Related Compounds in Raw Materials and Tablets

1994 ◽  
Vol 77 (6) ◽  
pp. 1447-1453 ◽  
Author(s):  
Pauline M Lacrok ◽  
Norman M Curran ◽  
Wing-Wah Sy ◽  
Dennis K J Goreck ◽  
Pierre Thibault ◽  
...  
Keyword(s):  
Raw Materials ◽  
Chromatographic Determination ◽  
Raw Material ◽  
Liquid Chromatographic Method ◽  
Limit Of Quantitation ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic ◽  
Amiodarone Hydrochloride ◽  
Related Compounds

Abstract A liquid chromatographic method for the determination of amiodarone hydrochloride and 10 related compounds in drug raw material and for assay of drug in tablets was developed. The method specifies a 3 jxm Hypersil nitrile column (150 × 4.6 mm), a mobile phase of 1 + 1 acetonitrile–ammonium acetate buffer (0.1 M adjusted to pH 6.0 with 0.1 M acetic acid), a flow rate of 1 mL/min, and detection at 240 nm. The lower limit of quantitation of the related compounds is 0.02% or less. Drug contents in 2 raw material samples were 100.1 and 99.9% and ranged from 98.2 to 99.4% in 3 tablet formulations. Impurity levels in 2 samples of raw material from different manufacturers were ca 0.4%. The presence of 3 of the known related compounds in these samples was confirmed by liquid chromatographymass spectrometry. The method applied to raw materials was evaluated by a second laboratory and found to be satisfactory.

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