Isolation and Molecular Characterization of Mycoplasma gallisepticum and Mycoplasma synoviae in Chickens in Sudan

The current study described the isolation and molecular detection of Mycoplasma gallisepticum (Mg) and Mycoplasma synoviae from tracheal swabs of diseased birds showing signs of respiratory distress in selected commercial (layer and broiler) farms and from yolk and an open air of pens of vaccinated breeder flocks in Sudan. A number of 45 Mycoplasma isolates were recovered from chickens in Khartoum, Gezira, and Equatoria states in Sudan. Of these, eight Mg and three Ms isolates were identified using growth inhibition and rapid serum agglutination (RSA) tests. The conventional PCR technique was applied to amplify 140 bp and 720 bp DNA fragments for the Mg and Ms, respectively. This research confirmed vertical and horizontal transmission of Mg from breeder farms through detection of Mg in yolk of fertile eggs and an air of pens despite previous vaccination. PCR is considered a rapid, sensitive, and cheap method and it will improve the diagnosis of Mycoplasma in chickens.

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Molecular detection and characterization of Mycoplasma gallisepticum and Mycoplasma synoviae strains in backyard poultry in Italy

Poultry Science ◽

10.1016/j.psj.2019.12.020 ◽

2020 ◽

Vol 99 (2) ◽

pp. 719-724 ◽

Cited By ~ 4

Author(s):

Viviana Felice ◽

Caterina Lupini ◽

Giulia Mescolini ◽

Flavio Silveira ◽

Alessandro Guerrini ◽

...

Keyword(s):

Molecular Detection ◽

Mycoplasma Gallisepticum ◽

Mycoplasma Synoviae ◽

Backyard Poultry

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Influences of swab types and storage temperatures on isolation and molecular detection of Mycoplasma gallisepticum and Mycoplasma synoviae

Avian Pathology ◽

10.1080/03079457.2019.1675865 ◽

2019 ◽

Vol 49 (1) ◽

pp. 106-110

Author(s):

Christopher Ball ◽

Viviana Felice ◽

Yichao Ding ◽

Anne Forrester ◽

Elena Catelli ◽

...

Keyword(s):

Molecular Detection ◽

Mycoplasma Gallisepticum ◽

Mycoplasma Synoviae ◽

And Storage ◽

Storage Temperatures

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Molecular detection and genetic characterization of Mycoplasma gallisepticum, Mycoplama synoviae, and infectious bronchitis virus in poultry in Myanmar

BMC Veterinary Research ◽

10.1186/s12917-019-2018-2 ◽

2019 ◽

Vol 15 (1) ◽

Cited By ~ 3

Author(s):

Sotaro Fujisawa ◽

Shiro Murata ◽

Masaki Takehara ◽

Ken Katakura ◽

Myint Myint Hmoon ◽

...

Keyword(s):

Infectious Bronchitis Virus ◽

Molecular Detection ◽

Genetic Characterization ◽

Mycoplasma Gallisepticum ◽

Infectious Bronchitis

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Isolation and Molecular Detection of Mycoplasma gallisepticum in Commercial Layer Chickens in Sylhet, Bangladesh

World s Veterinary Journal ◽

10.54203/scil.2021.wvj78 ◽

2021 ◽

Vol 11 (4) ◽

pp. 614-620

Author(s):

Md Shamsul Islam Basit ◽

Mohammad Al Mamun ◽

Md. Masudur Rahman ◽

Monira Noor

Keyword(s):

Molecular Detection ◽

Mycoplasma Gallisepticum ◽

Dead Layer ◽

Conventional Pcr ◽

Tissue Samples ◽

Entire Study ◽

Air Sacs ◽

Cultural Isolation ◽

Considerable Impact ◽

Layer Chickens

Mycoplasma gallisepticum induced poultry diseases are associated with a huge economic crisis and have a considerable impact on the poultry industry worldwide. The aim of the current study was to isolate and perform molecular detection of MG circulating pathogenic strain in the commercial layer farms in the Sylhet district of Bangladesh. The entire study was conducted from January 2018 to January 2019 at three Upazilas of Sylhet district in Bangladesh. A total of 50 dead layer chickens (indicating signs of respiratory distress before death) were collected randomly from 15 different layer farms. The tissue samples, such as air sacs, trachea, and lungs, were taken from suspected dead chickens. Both cultural and PCR-based techniques were applied to identify Mycoplasma from tissue samples. The conventional PCR technique was implemented to amplify 185 bp DNA fragments for the MG. Out of 50 samples, 36% (18/50) and 70% (35/50) of MG were identified by cultural method and PCR, respectively. Based on the results of the study, it can be concluded that PCR is an easier, more sensitive, and less time-consuming method for the early diagnosis of MG in chickens, compared to cultural isolation and hence can lower the economic burden to poultry farmers caused by this disease.

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Detection of antibodies against Mycoplasma gallisepticum and Mycoplasma synoviae in day-old broiler chicks and broilers

Journal of the Hellenic Veterinary Medical Society ◽

10.12681/jhvms.15357 ◽

2018 ◽

Vol 53 (1) ◽

pp. 33

Author(s):

G. K. GEORGIADES (Γ.Κ. ΓΕΩΡΓΙΑΔΗΣ)

Keyword(s):

Respiratory Disease ◽

Respiratory Diseases ◽

Diagnostic Method ◽

Mycoplasma Gallisepticum ◽

Agglutination Test ◽

Broiler Chicks ◽

Broiler Chick ◽

Mycoplasma Synoviae ◽

Serum Agglutination

During the last decade, in die Clinic of Poultry Diseases, 882 sera were examined for the detection of antibodies against Mycoplasma gallisepticum (M.g.) and Mycoplasma synoviae (M.s.), which are usually involved in the respiratory diseases of broilers. Out of these sera, 188 derived from day-old broiler chicks and 694 from broilers with respiratory disease. Rapid serum agglutination test was used as diagnostic method. Among day-old broiler chick sera, 40 (21.27%) were M.g. positive, while 76 (40.42%) were M.s. positive. Among broiler sera, 133 (19.16%) were M.g. positive, while 356 (51.29%) were M.s. positive. The results of the present study show that the prevalence of the M.s. positive sera is significantly higher (P<0.001) than this of the M.g. positive sera, not only in day-old broiler chicks, but also in broilers, which indicates the greater importance of M.s. in the occurrence of respiratory disease in these birds.

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Phylogeny Characterization of Seb Gene Encoding Enterotoxins in Staphylococcus aureus Isolated from Raw Milk and Cheese

International Journal of Drug Delivery Technology ◽

10.25258/ijddt.v9i3.13 ◽

2019 ◽

Vol 9 (o3) ◽

Author(s):

Fatima N. Aziz ◽

Laith Abdul Hassan Mohammed-Jawad

Keyword(s):

Staphylococcus Aureus ◽

Food Poisoning ◽

Raw Milk ◽

Staphylococcal Enterotoxin B ◽

Human Pathogen ◽

Conventional Pcr ◽

Biochemical Tests ◽

Specific Primers ◽

Gene Encoding

Food poisoning due to the bacteria is a big global problem in economically and human's health. This problem refers to an illness which is due to infection or the toxin exists in nature and the food that use. Milk is considered a nutritious food because it contains proteins and vitamins. The aim of this study is to detect and phylogeny characterization of staphylococcal enterotoxin B gene (Seb). A total of 200 milk and cheese samples were screened. One hundred ten isolates of Staphylococcus aureus pre-confirmed using selective and differential media with biochemical tests. Genomic DNA was extracted from the isolates and the SEB gene detects using conventional PCR with specific primers. Three staphylococcus aureus isolates were found to be positive for Seb gene using PCR and confirmed by sequencing. Sequence homology showed variety range of identity starting from (100% to 38%). Phylogenetic tree analyses show that samples (6 and 5) are correlated with S. epidermidis. This study discovered that isolates (A6-RLQ and A5-RLQ) are significantly clustered in a group with non- human pathogen Staphylococcus agnetis.

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