scholarly journals Isolation and Molecular Detection of Mycoplasma gallisepticum in Commercial Layer Chickens in Sylhet, Bangladesh

2021 ◽  
Vol 11 (4) ◽  
pp. 614-620
Author(s):  
Md Shamsul Islam Basit ◽  
Mohammad Al Mamun ◽  
Md. Masudur Rahman ◽  
Monira Noor
Keyword(s):  
Molecular Detection ◽  
Mycoplasma Gallisepticum ◽  
Dead Layer ◽  
Conventional Pcr ◽  
Tissue Samples ◽  
Entire Study ◽  
Air Sacs ◽  
Cultural Isolation ◽  
Considerable Impact ◽  
Layer Chickens

Mycoplasma gallisepticum induced poultry diseases are associated with a huge economic crisis and have a considerable impact on the poultry industry worldwide. The aim of the current study was to isolate and perform molecular detection of MG circulating pathogenic strain in the commercial layer farms in the Sylhet district of Bangladesh. The entire study was conducted from January 2018 to January 2019 at three Upazilas of Sylhet district in Bangladesh. A total of 50 dead layer chickens (indicating signs of respiratory distress before death) were collected randomly from 15 different layer farms. The tissue samples, such as air sacs, trachea, and lungs, were taken from suspected dead chickens. Both cultural and PCR-based techniques were applied to identify Mycoplasma from tissue samples. The conventional PCR technique was implemented to amplify 185 bp DNA fragments for the MG. Out of 50 samples, 36% (18/50) and 70% (35/50) of MG were identified by cultural method and PCR, respectively. Based on the results of the study, it can be concluded that PCR is an easier, more sensitive, and less time-consuming method for the early diagnosis of MG in chickens, compared to cultural isolation and hence can lower the economic burden to poultry farmers caused by this disease.

Detection of Mycoplasma galliseptium infection in chickens from Tamil Nadu State of India

2018 ◽  
Author(s):  
K. Manimaran ◽  
Adarsh Mishra ◽  
S. Hemalatha ◽  
K. Karthik ◽  
P.I. Ganesan
Keyword(s):  
Tamil Nadu ◽  
Molecular Detection ◽  
Age Groups ◽  
Chronic Respiratory Disease ◽  
Rrna Gene ◽  
Histopathological Changes ◽  
Tissue Samples ◽  
Air Sacs ◽  
Tamil Nadu State ◽  
Pcr Targeting

Mycoplasma gallisepticum (MG) is one of the major respiratory tract pathogens affecting chickens. It causes Chronic Respiratory Disease (CRD) among chickens of various age groups. The present study describes the isolation, molecular detection and histopathological changes associated with CRD in chickens. A total of 790 samples viz., trachea, lungs and air sacs were collected from chickens showing the symptoms of CRD from different parts of Tamil Nadu state. All the samples were processed for isolation and molecular detection of MG. A total of 91 samples were found positive by isolation and 105 samples were found positive through MG specific PCR targeting 16S rRNA gene. The histopathological changes in tissue samples of trachea, sinuses, air sacs and lungs collected from naturally infected M. gallisepticum infection were suggestive of subacute to chronic nature of infection. Though isolation is considered to be a gold standard, still PCR is a rapid, sensitive and cheap method for early diagnosis of MG which can help poultry farmers to avoid severe economic loss.

scholarly journals Isolation and Molecular Characterization of Mycoplasma gallisepticum and Mycoplasma synoviae in Chickens in Sudan

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
Khalda A. Khalifa ◽  
Egbal Sidahmed Abdelrahim ◽  
Magdi Badwi ◽  
Amal M. Mohamed
Keyword(s):  
Respiratory Distress ◽  
Growth Inhibition ◽  
Molecular Detection ◽  
Horizontal Transmission ◽  
Mycoplasma Gallisepticum ◽  
Conventional Pcr ◽  
Mycoplasma Synoviae ◽  
Cheap Method ◽  
Serum Agglutination

The current study described the isolation and molecular detection of Mycoplasma gallisepticum (Mg) and Mycoplasma synoviae from tracheal swabs of diseased birds showing signs of respiratory distress in selected commercial (layer and broiler) farms and from yolk and an open air of pens of vaccinated breeder flocks in Sudan. A number of 45 Mycoplasma isolates were recovered from chickens in Khartoum, Gezira, and Equatoria states in Sudan. Of these, eight Mg and three Ms isolates were identified using growth inhibition and rapid serum agglutination (RSA) tests. The conventional PCR technique was applied to amplify 140 bp and 720 bp DNA fragments for the Mg and Ms, respectively. This research confirmed vertical and horizontal transmission of Mg from breeder farms through detection of Mg in yolk of fertile eggs and an air of pens despite previous vaccination. PCR is considered a rapid, sensitive, and cheap method and it will improve the diagnosis of Mycoplasma in chickens.

scholarly journals Molecular detection of vector borne pathogens in anemic and thrombocytopenic dogs in southern Brazil

2018 ◽  
Vol 27 (4) ◽  
pp. 505-513 ◽  
Author(s):  
Anna Cláudia Baumel Mongruel ◽  
Priscila Ikeda ◽  
Keyla Carstens Marques de Sousa ◽  
Jyan Lucas Benevenute ◽  
Margarete Kimie Falbo ◽  
...  
Keyword(s):  
18S Rrna ◽  
Molecular Detection ◽  
Phylogenetic Analyses ◽  
Southern Brazil ◽  
Conventional Pcr ◽  
Pathogenic Potential ◽  
Vector Borne ◽  
Pcr Assays ◽  
Etiological Agents ◽  
Positive Results

Abstract Arthropod-borne pathogens are medically important because of their ability to cause diseases in their hosts. The purpose of this study was to detect the occurrence of Ehrlichia spp., piroplasmids and Hepatozoon spp. in dogs with anemia and thrombocytopenia in southern Brazil. EDTA-whole blood was collected from 75 domestic dogs presenting anemia or/and thrombocytopenia from Guarapuava, state of Paraná, Brazil. DNA samples were subjected to conventional PCR assays for Ehrlichia spp. (dsb), piroplasmids (18S rRNA) and Hepatozoon spp. (18S rRNA), followed by sequencing and phylogenetic analyses. Among the 75 dogs, one (1.33%) was positive for Hepatozoon sp. and six (8%) were positive for piroplasmids in 18S rRNA cPCR assays. None of the dogs showed positive results in Ehrlichia spp.-cPCR targeting dsb gene. The phylogenetic analyses revealed that three piroplasm sequences were clustered with Rangellia vitalii, while one sequence was grouped with B. vogeli. The only sequence obtained from Hepatozoon spp.-PCR protocol was pooled with H. canis. Therefore, there is urgent need for differential molecular diagnosis of the two piroplasm species cited as etiological agents in clinical cases of canine hemoparasitic diseases, given the higher pathogenic potential of R. vitalii than of B. vogeli.

scholarly journals Influences of swab types and storage temperatures on isolation and molecular detection of Mycoplasma gallisepticum and Mycoplasma synoviae

2019 ◽  
Vol 49 (1) ◽  
pp. 106-110
Author(s):  
Christopher Ball ◽  
Viviana Felice ◽  
Yichao Ding ◽  
Anne Forrester ◽  
Elena Catelli ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Mycoplasma Gallisepticum ◽  
Mycoplasma Synoviae ◽  
And Storage ◽  
Storage Temperatures

Isolation and Phylogenetic Analysis of Avian Mycoplasmas from Poultry Affected with Respiratory Infections in India

2020 ◽  
Author(s):  
Y. Singh ◽  
P. Tomar ◽  
N. K. Mahajan ◽  
N. Jindal
Keyword(s):  
Phylogenetic Analysis ◽  
Broiler Chicken ◽  
Respiratory Infections ◽  
Intergenic Spacer ◽  
Mycoplasma Gallisepticum ◽  
Epidemiological Investigation ◽  
Wild Type ◽  
Tissue Samples ◽  
Growth Inhibition Test ◽  
Intergenic Spacer Region

Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) are the two most pathogenic avian mycoplasmas. In the present study, examination of 92 pooled tissue samples from broiler chicken of 92 different poultry flocks of Haryana (India) exhibiting respiratory infections resulted in isolation of 13 (14%) Mollicutes. Based on biochemical reactions, growth inhibition test, PCR and/or sequencing, 8 (8.6%) isolates could be characterized as MG, 1 (1.08%) as MS, 3 (3.24%) as M. gallinarum and 1 (1.08%) as Acholeplasma laidlawii. The phylogenetic analysis using Intergenic spacer region (IGSR) of these MG isolates revealed that they clustered with USA strain whereas the vaccine strains were in different clade. Single locus sequence typing (SLST) revealed considerable nucleotides variation between 8 MG isolates and vaccine strains. Conclusively, Sequencing of IGSR region of MG can be used as a valuable epidemiological investigation tool for the differentiation of wild-type MG strains from vaccine strains.

scholarly journals Pooled molecular occurrence of Mycoplasma gallisepticum and Mycoplasma synoviae in poultry: A systematic review and meta-analysis

2021 ◽  
Author(s):  
Miguel Angel Chaidez-Ibarra ◽  
Diana Zuleika Velazquez ◽  
Idalia Enriquez-Verdugo ◽  
Nohemi Castro del Campo ◽  
Miguel Angel Rodriguez-Gaxiola ◽  
...  
Keyword(s):  
Systematic Review ◽  
Molecular Detection ◽  
Meta Analysis ◽  
Ambient Air ◽  
Mycoplasma Gallisepticum ◽  
Chronic Respiratory Disease ◽  
Control Measures ◽  
Sub Saharan Africa ◽  
Ambient Air Pollution ◽  
Sub Saharan

Worldwide, Mycoplasma gallisepticum (MG) and M. synoviae (MS) are the main agents responsible for chronic respiratory disease in poultry. Therefore, we conducted a systematic review and meta-analysis to estimate their occurrence. We searched electronic databases to find peer-reviewed publications reporting the molecular detection of MG and MS in poultry and used meta-analysis to estimate their pooled occurrence (combined flock and individual), aggregating results at the regional and national levels. We performed a subgroup meta-analysis for subpopulations (broilers, layers, breeders, and diverse poultry including turkeys, ducks, and ostriches) and used meta-regression with categorical modifiers. We retrieved 2,294 publications from six electronic databases and included 85 publications from 33 countries that reported 62 studies with 22,162 samples for MG and 48 studies with 26,413 samples for MS. The pooled occurrence was 38.4% (95% CI: 23.5-54.5) for MS and 27.0% (20.4-34.2) for MG. Among regions, Europe and Central Asia had the lowest occurrence for both pathogens, while MG and MS were highly prevalent in South Asia and sub-Saharan Africa, respectively. MG occurrence was higher in Algeria, Saudi Arabia, and Sudan, whereas China, Egypt, and Ethiopia reported a higher occurrence of MS. MS and MG were more prevalent in the breeders and layers (62.6% and 31.2%, respectively) than in diverse poultry. The year of publication, the sample size, and the level of ambient air pollution (measured indirectly by PM2.5) were associated with the occurrence of both mycoplasmas. Our study revealed a high and heterogeneous occurrence of MG and MS and justifies the need for an early detection and improved control measures to reduce the spread of these pathogens.

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