scholarly journals Study on the Interaction between Cadmium Sulphide Nanoparticles and Proteins by Resonance Rayleigh Scattering Spectra

2013 ◽  
Vol 2013 ◽  
pp. 1-7
Author(s):  
Weiwei Zhu ◽  
Qi Wang ◽  
Dan Su
Keyword(s):  
Human Serum Albumin ◽  
Bovine Serum Albumin ◽  
Optimum Condition ◽  
Serum Albumin ◽  
Rayleigh Scattering ◽  
Cadmium Sulphide ◽  
Resonance Rayleigh Scattering ◽  
Hydrophobic Force ◽  
Scattering Spectra

The interaction of cadmium sulphide nanoparticles[(CdS)n]with proteins has been studied by resonance Rayleigh scattering spectra (RRS). Below the isoelectric point, proteins such as bovine serum albumin (BSA), human serum albumin (HSA), lysozyme (Lys), hemoglobin (HGB), and ovalbumin (OVA) can bind withCdSnto form macromolecules by virtue of electrostatic attraction and hydrophobic force. It can result in the enhancement of resonance Rayleigh scattering spectra (RRS) intensity. Their maximum scattering peaks were 280 nm, and there was a smaller peak at 370 nm. The scattering enhancement (ΔIRRS) is directly proportional to the concentration of proteins. A new RRS method for the determination of trace proteins using uncappedCdSnnanoparticles probe has been developed. The detection limits are 19.6 ng/mL for HSA, 16.7 ng/mL for BSA, 18.5 ng/mL for OVA, 80.2 ng/mL for HGB, and 67.4 ng/mL for Lys, separately. In this work, the optimum condition of reaction, the effect of foreign, and the analytical application had been investigated.

Resonance Rayleigh Scattering Determination of Trace Hemin Basing on Aptamer-Modified Nanogold Catalysis of HAuCl4-Vitamine C

2013 ◽  
Vol 787 ◽  
pp. 400-403
Author(s):  
Jin Chao Dong ◽  
Ai Hui Liang ◽  
Zhi Liang Jiang
Keyword(s):  
Detection Limit ◽  
Rayleigh Scattering ◽  
Resonance Rayleigh Scattering ◽  
Serum Samples ◽  
Buffer Solution ◽  
Strong Resonance ◽  
Scattering Spectra ◽  
Vitamine C ◽  
Nanogold Catalysis

Hemin aptamer was used to modify gold nanoparticles (AuNPs) to obtain a stable aptamer-nanogold probe (AussDNA). In the condition of pH 8.0 Tris-HCl buffer solution containing 50mmol/L NaCl, the substrate chain of AussDNA was cracked by hemin to produce a short single-stranded DNA(ssDNA) and then further combined with hemin to form a stable hemin-ssDNA conjugate. The AuNPs released from AussDNA would be aggregated in the condition of 50mmol/L NaCl and exhibited a strong resonance Rayleigh scattering (RRS) peak at 368nm. Under the selected conditions, the increased RRS intensity (ΔI368nm) was linear to hemin concentration in the range of 5-750nmol/L, with a detection limit of 66 pmol/L. This RRS method was applied to determination of residual hemin in serum samples, with satisfactory results. The remnant AussDNA in the solution exhibited a strong catalytic activity on the gold particle reaction of HAuCl4-vitamine C (VC) that can be monitored by RRS technique at 368 nm. When the hemin concentration increased, the AussDNA decreased, the catalysis decreased, and the RRS intensity at 368nm decreased. The decreased RRS intensity ΔI368nmwas linear to the hemin concentration in the range of 1-200nmol/L, with a detection limit of 54 pmol/L. Accordingly, a sensitivity, selectivity, and simplicity new method of resonance Rayleigh scattering spectra to detect hemin using aptamer-modified nanogold as catalyst was established.

A new and simple resonance Rayleigh scattering method for human serum albumin using graphite oxide as probe

Luminescence ◽  
2012 ◽  
Vol 28 (6) ◽  
pp. 842-846 ◽  
Author(s):  
Shengmian Wang ◽  
Lili Xu ◽  
Lisheng Wang ◽  
Aihui Liang ◽  
Zhiliang Jiang
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Graphite Oxide ◽  
Rayleigh Scattering ◽  
Resonance Rayleigh Scattering ◽  
Scattering Method
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