scholarly journals A Histopathological and Immunohistochemical Analysis of Ameloblastic Fibrodentinoma

2013 ◽  
Vol 2013 ◽  
pp. 1-7
Author(s):  
Ronell Bologna-Molina ◽  
Sirced Salazar-Rodríguez ◽  
Ana María Bedoya-Borella ◽  
Ramón Gil Carreón-Burciaga ◽  
Gabriel Tapia-Repetto ◽  
...  
Keyword(s):  
Histone H3 ◽  
Immunohistochemical Analysis ◽  
Odontogenic Tumor ◽  
Rare Entity ◽  
Ki 67 ◽  
Dental Papilla ◽  
Odontogenic Epithelium ◽  
Ameloblastic Fibroma ◽  
Large Panel

Ameloblastic fibrodentinoma (AFD) is considered a mixed odontogenic tumor that is characterized by conserved epithelial and ectomesenchymal neoplastic components. AFD is composed of long narrow cords and islands of odontogenic epithelium; the epithelial strands lie in a myxoid cell-rich ectomesenchymal tissue with stellate-shaped fibroblasts that exhibit long slender cytoplasmic extensions that resemble dental papilla. The lesions show the presence of dysplastic dentin. Although AFD is a rare entity and its very existence is not completely accepted, based on the extent of histodifferentiation, it is considered to represent a stage between ameloblastic fibroma and ameloblastic fibroodontoma. This study aimed to provide a histopathological and immunohistochemical characterization of this infrequent tumor. A large panel of antibodies including amelogenin, Ck 19, calretinin, syndecan-1, E-cadherin, MSH2, histone H3, and Ki-67 was used to illustrate the nature of the tumor.

scholarly journals A case involving the spontaneous reduction of an ameloblastic fibroma

2017 ◽  
Vol 2 (3) ◽  
pp. 194
Author(s):  
Yasuaki Mekaru ◽  
Ueda Gousei ◽  
Higa Tsutomu ◽  
Kouchi Masato ◽  
Tatetsu Masaharu ◽  
...  
Keyword(s):  
Histological Evaluation ◽  
Epithelial Tissue ◽  
Mandibular Ramus ◽  
Ki 67 ◽  
X Ray ◽  
Spontaneous Reduction ◽  
Odontogenic Epithelium ◽  
Ameloblastic Fibroma ◽  
Malignant Changes ◽  
The Right

Objective: Ameloblastic fibroma is an uncommon type of mixed odontogenic tumor. It is caused by the growth of the odontogenic epithelium and mesenchymal tissue and can have adverse effects on the formation of dental structures. It usually arises in the mandible, but cases involving the mandibular ramus are rare.Methods: Here, we report a case of ameloblastic fibroma in the right mandibular ramus involving a 15-year-old male patient. According to panoramic X-ray examinations, the tumor shrank spontaneously in the 3 years before surgery (from approximately 80mm×55mmto 50mm×25mm).Results: A histological evaluation revealed the proliferation of odontogenic epithelial tissue and mesenchymal elements. The Ki-67 index of the lesion was 0%.Conclusion: Cases of ameloblastic fibroma involving patients aged ≥ 22 years old are very likely to recur and undergo malignant changes.

scholarly journals Cell proliferation and apoptosis in ameloblastomas and keratocystic odontogenic tumors

2012 ◽  
Vol 23 (2) ◽  
pp. 91-96 ◽  
Author(s):  
Fabrício Rezende Amaral ◽  
Gláucia Cardoso Paixão Mateus ◽  
Lucas Alves Bonisson ◽  
Bruno Augusto Benevenuto de Andrade ◽  
Ricardo Alves Mesquita ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Odontogenic Tumor ◽  
Proliferation Index ◽  
Methyl Green ◽  
Keratocystic Odontogenic Tumor ◽  
Ki 67 ◽  
Odontogenic Epithelium ◽  
Significant Difference ◽  
High Proliferative Activity ◽  
Peripheral Cells

A high proliferative activity of the odontogenic epithelium in ameloblastoma (AM) and keratocystic odontogenic tumor (KOT) has been demonstrated. However, no previous study has simultaneously evaluated cell proliferation and apoptotic indexes in AM and KOT, comparing both lesions. The aim of this study was to assess and compare cell proliferation and apoptotic rates between these two tumors. Specimens of 11 solid AM and 11 sporadic KOT were evaluated. The proliferation index (PI) was assessed by immunohistochemical detection of Ki-67 and the apoptotic index (AI) by methyl green-pyronine and in situ DNA nick end-labelling methods. KOT presented a higher PI than AM (p<0.05). No statistically significant difference was found in the AI between AM and KOT. PI and AI were higher in the peripheral cells of AM and respectively in the suprabasal and superficial layers of KOT. In conclusion, KOT showed a higher cell proliferation than AM and the AI was similar between these tumors. These findings reinforce the classification of KOT as an odontogenic tumor and should contribute to its aggressive clinical behavior.

scholarly journals Maxillary ameloblastic fibroma: a case report

2011 ◽  
Vol 22 (2) ◽  
pp. 171-174 ◽  
Author(s):  
Daniela Otero Pereira da Costa ◽  
Adriana Terezinha Neves Novellino Alves ◽  
Mônica Diuna Calasans-Maia ◽  
Ricardo Lopes da Cruz ◽  
Simone de Queiroz Chaves Lourenço
Keyword(s):  
Panoramic Radiograph ◽  
Histopathological Diagnosis ◽  
Oral Lesions ◽  
Orthodontic Appliance ◽  
Dental Papilla ◽  
Permanent Molar ◽  
Odontogenic Epithelium ◽  
Ameloblastic Fibroma ◽  
Fixed Orthodontic Appliance

Ameloblastic fibroma is a relatively rare benign odontogenic tumor in which both the epithelial and ectomesenchymal components are neoplastic. An 8-year-old Caucasian boy was referred to the dentist for evaluation of failed eruption of the maxillary left first molar. The panoramic radiograph showed a well-circumscribed unilocular radiolucency involving an unerupted maxillary left first permanent molar. The lesion was enucleated and the material was sent for histopathologic examination. Microscopically, it was composed by cords and islands of odontogenic epithelium in a myxoid cell-rich stroma that closely resemble the dental papilla with histopathological diagnosis of ameloblastic fibroma. After 24 months of follow-up no recurrence was observed and the maxillary left first molar erupted spontaneously through the buccal mucosa and was aligned with a fixed orthodontic appliance. This case emphasized the importance of careful differential diagnosis of intraosseous oral lesions and reported a rarity of the lesion and its atypical location.

scholarly journals REMY: A platform for the rapid interrogation of epigenome modifications on yeast

2021 ◽  
Author(s):  
Alison C. Waldman ◽  
Balaji M. Rao ◽  
Albert J. Keung
Keyword(s):  
Recombinant Protein ◽  
Recombinant Protein Production ◽  
Protein Production ◽  
Histone Acetyltransferase ◽  
Surface Display ◽  
Histone H3 ◽  
Proof Of Concept ◽  
Large Panel ◽  
Chromatin Biology

AbstractHistone proteins are decorated with a combinatorially and numerically diverse set of biochemical modifications. Here we describe a versatile and scalable platform termed Rapid interrogation of Epigenome Modifications using Yeast surface display (REMY), which enables efficient characterization of histone modifications without the need for recombinant protein production. As proof-of-concept, we first used REMY to rapidly profile the histone H3 and H4 residue writing specificities of the human histone acetyltransferase, p300. Subsequently, we used REMY to screen a large panel of commercially available anti-acetylation antibodies for their specificities, identifying many suitable and unsuitable reagents. Further, use of REMY enabled efficient mapping of the large binary crosstalk space between acetylated residues on histones H3 and H4, and uncovered previously unreported residue interdependencies affecting p300 activity. Our results show that REMY is a useful tool that can advance our understanding of chromatin biology by enabling efficient interrogation of the complexity of epigenome modifications.

scholarly journals The Role of Immunohistochemical Markers for the Diagnosis and Prognosis of Adrenocortical Neoplasms

2021 ◽  
Vol 11 (3) ◽  
pp. 208
Author(s):  
Anna Angelousi ◽  
Georgios Kyriakopoulos ◽  
Fani Athanasouli ◽  
Anastasia Dimitriadi ◽  
Eva Kassi ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Cox Regression ◽  
P53 Protein ◽  
Immunohistochemical Analysis ◽  
Ki 67 ◽  
Diagnosis And Prognosis ◽  
Immunohistochemical Markers ◽  
Small Series ◽  
Morphological Criteria ◽  
Survival And Development

Adrenal cortical carcinoma (ACC) is a rare cancer with poor prognosis that needs to be distinguished from adrenocortical adenomas (ACAs). Although, the recently developed transcriptome analysis seems to be a reliable tool for the differential diagnosis of adrenocortical neoplasms, it is not widely available in clinical practice. We aim to evaluate histological and immunohistochemical markers for the distinction of ACCs from ACAs along with assessing their prognostic role. Clinical data were retrospectively analyzed from 37 patients; 24 archived, formalin-fixed, and paraffin-embedded ACC samples underwent histochemical analysis of reticulin and immunohistochemical analysis of p27, p53, Ki-67 markers and were compared with 13 ACA samples. Weiss and Helsinki scores were also considered. Kaplan−Meier and univariate Cox regression methods were implemented to identify prognostic effects. Altered reticulin pattern, Ki-67% labelling index and overexpression of p53 protein were found to be useful histopathological markers for distinguishing ACAs from ACCs. Among the studied markers, only pathological p53 nuclear protein expression was found to reach statistically significant association with poor survival and development of metastases, although in a small series of patients. In conclusion, altered reticulin pattern and p53/Ki-67 expression are useful markers for distinguishing ACCs from ACAs. Immunohistopathology alone cannot discriminate ACCs with different prognosis and it should be combined with morphological criteria and transcriptome analysis.

Hybrid odontogenic tumor of calcifying odontogenic cyst and ameloblastic fibroma

2004 ◽  
Vol 98 (1) ◽  
pp. 80-84 ◽  
Author(s):  
Jung Hoon Yoon ◽  
Hyung Jun Kim ◽  
Jong In Yook ◽  
In Ho Cha ◽  
Gary L Ellis ◽  
...  
Keyword(s):  
Odontogenic Cyst ◽  
Odontogenic Tumor ◽  
Calcifying Odontogenic Cyst ◽  
Ameloblastic Fibroma

scholarly journals Clear Cell Carcinomas of the Mullerian System: Does the Pathogenesis Vary Depending on Their Nuclear Grade and Their Association with Endometriosis? An Immunohistochemical Analysis

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Ahmad Alduaij ◽  
Katrine Hansen ◽  
Tahreem A. Karim ◽  
Cunxian Zhang ◽  
Michelle M. Lomme ◽  
...  
Keyword(s):  
Clear Cell ◽  
Immunohistochemical Analysis ◽  
Nuclear Grade ◽  
High Grade ◽  
Ki 67 ◽  
High Nuclear Grade ◽  
Positive Immunostaining

Clear cell carcinomas (CCC) of the mullerian system are considered high grade tumors, but morphologically, the cells of CCC show both low and high grade features. The aims of the current study were to categorize CCC into low and high nuclear grade types, correlate their association with endometriosis, and then observe possible variations in pathogenesis based on their expression of p53 and Ki-67. We studied 41 pure mullerian CCCs and designated each as either a high (HNG) or low (LNG) nuclear grade tumor. Morphologically, 17 (41%) CCCs were LNG and 24 (59%) were HNG. Nine (38%) HNG and 2 (12%) LNG tumors showed positive immunostaining with p53. Endometriosis was associated with 8 (47%) LNG tumors and 8 (33%) HNG CCCs. Of the 11 cases with p53 alteration, 4 (1 LNG and 3 HNG) were associated with endometriosis. Conclusions: HNG CCCs, irrespective of their association with endometriosis, have alterations of p53. In general, LNG ovarian and endometrial CCCs, irrespective of their association with endometriosis/adenomyosis, are less likely to show p53 alteration. It appears that mullerian CCCs may have variable pathogenesis depending on their nuclear grade and association with endometriosis. A larger study is needed to validate these findings.

Polyclonal human rheumatoid factors cross-reacting with histone H3: Characterization of an idiotope on the H3 binding site

1990 ◽  
Vol 10 (4) ◽  
pp. 211-219 ◽  
Author(s):  
Thierry Martin ◽  
Anne Marie Knapp ◽  
Sylvianne Muller ◽  
Jean Louis Pasquali
Keyword(s):  
Binding Site ◽  
Histone H3 ◽  
Rheumatoid Factors
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