scholarly journals Cell proliferation and apoptosis in ameloblastomas and keratocystic odontogenic tumors

2012 ◽  
Vol 23 (2) ◽  
pp. 91-96 ◽  
Author(s):  
Fabrício Rezende Amaral ◽  
Gláucia Cardoso Paixão Mateus ◽  
Lucas Alves Bonisson ◽  
Bruno Augusto Benevenuto de Andrade ◽  
Ricardo Alves Mesquita ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Odontogenic Tumor ◽  
Proliferation Index ◽  
Methyl Green ◽  
Keratocystic Odontogenic Tumor ◽  
Ki 67 ◽  
Odontogenic Epithelium ◽  
Significant Difference ◽  
High Proliferative Activity ◽  
Peripheral Cells

A high proliferative activity of the odontogenic epithelium in ameloblastoma (AM) and keratocystic odontogenic tumor (KOT) has been demonstrated. However, no previous study has simultaneously evaluated cell proliferation and apoptotic indexes in AM and KOT, comparing both lesions. The aim of this study was to assess and compare cell proliferation and apoptotic rates between these two tumors. Specimens of 11 solid AM and 11 sporadic KOT were evaluated. The proliferation index (PI) was assessed by immunohistochemical detection of Ki-67 and the apoptotic index (AI) by methyl green-pyronine and in situ DNA nick end-labelling methods. KOT presented a higher PI than AM (p<0.05). No statistically significant difference was found in the AI between AM and KOT. PI and AI were higher in the peripheral cells of AM and respectively in the suprabasal and superficial layers of KOT. In conclusion, KOT showed a higher cell proliferation than AM and the AI was similar between these tumors. These findings reinforce the classification of KOT as an odontogenic tumor and should contribute to its aggressive clinical behavior.

scholarly journals Expression of Osteopontin and Cyclooxygenase-2 in relation to cellular proliferation, in non-tumor colonic mucosa, colonic adenomas and colon adenocarcinoma

2021 ◽  
Vol 10 (1) ◽  
pp. 3-15
Author(s):  
Inés Benedetti Padron ◽  
Paola Lecompte ◽  
Lía Barrios Garcia
Keyword(s):  
Cell Proliferation ◽  
Colonic Mucosa ◽  
Colon Adenocarcinoma ◽  
Cyclooxygenase 2 ◽  
Proliferation Index ◽  
Ki 67 ◽  
Colonic Adenomas ◽  
Significant Difference ◽  
Cox 2 ◽  
Cell Proliferation Index

Introduction: the participation of Cyclooxygenase-2 (COX-2) and Osteopontin has been postulated in the development of colon cancer, which play an important role in the progression and could be biomarkers for its prognosis, but their role remains controversial. Objective: to determine and to compare the expression of Osteopontin and COX-2 in non-tumor colonic mucosa, colonic adenomas and colon adenocarcinoma, in relation to the cell proliferation index. Methods: the immunohistochemical expression of COX-2, Osteopontin and Ki-67 in formalin fixed paraffin embedded tissue of non-tumor colonic mucosa, colonic adenomas and colon adenocarcinoma were determined and compared. Results: were included 65 cases: 19 of non-tumor colonic mucosa, 13 colonic adenomas and 33 colon adenocarcinomas. There was increased expression of Ki-67 in dysplastic and tumor cells. There was positive expression for COX-2 in adenomas (30.7%) and adenocarcinomas (27.3%), without significant difference between non- tumor colonic mucosa, adenomas and adenocarcinoma (p = 0.888). Osteopontin showed more frequent positivity in adenocarcinomas (72.7%) and adenomas (84.6%) than in non-tumor mucosa (10.5%), (p = <0.0001), without significant differences in its expression between subtypes and grades of adenoma dysplasia, nor between grades of differentiation, extension and proliferation of adenocarcinomas. There was a significant association between Osteopontin expression and the cell proliferation index. No association was observed between the expression of COX-2 and Osteopontin (p = 0.96). Conclusions: Osteopontin overexpression in colon adenocarcinoma and adenomas in comparison with non-tumor colonic mucosa, and its significant relationship with the cell proliferation index, constitutes additional evidence of its possible participation in the colonic carcinogenesis process.

Expression of β-Catenin in Hepatocellular Carcinoma

2001 ◽  
Vol 71 (3) ◽  
pp. 116-125
Author(s):  
Norina Basa ◽  
Daniela Lazar ◽  
Remus Cornea ◽  
Sorina Taban ◽  
Melania Ardelean ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Proliferation ◽  
Tumor Cell ◽  
Mitotic Index ◽  
Histological Grade ◽  
Proliferation Index ◽  
Tumor Cell Proliferation ◽  
Ki 67 ◽  
B Virus ◽  
Development And Evolution

Alteration of β-catenin expression is involved in the development and evolution of hepatocellular carcinoma (HCC); β-catenin is able to influence tumor cell proliferation. We analyzed the immunohistochemical (IHC) expression of β-catenin on a group of 32 patients diagnosed with HCC using the anti-β-catenin monoclonal antibody (clone E247). We correlated the expression of β-catenin with the proliferation index of Ki-67 (PI Ki-67), the mitotic index (MI) and other clinical and pathological features. We observed an altered β-catenin expression in 58.38% of all HCC cases. This expression was insignificantly correlated with tumor size (]5 cm) (p = 0.683), histological grade G1-G2 (p = 0.307), vascular invasion (p = 0.299) and advanced pT stage (p = 0.453); we obtained a significantly higher MI in HCC with altered β-catenin expression (p = 0.018), as compared to HCC without overexpression (1.66 � 1.37) (p = 0.038) and a PI Ki-67 of 22.49 � 20.1 and 28.24 � 18.2, respectively in tumors with altered β-catenin expression with insignificant differences compared to HCC without overexpression (25.95 � 15.2) (p = 0.682 and p = 0.731, respectively). According to the results we obtained, aberrant β-catenin expression in HCC was correlated with a high mitotic index, therefore playing an important role in tumor progression by stimulating tumor cell proliferation; non-nuclear β-catenin overexpression can have a pathological significance in HCC, especially in cases of HCC associated with hepatitis B virus (HBV) infection.

scholarly journals Association Between Radiographic Features and Cell Proliferation in Keratocystic Odontogenic Tumor

2015 ◽  
Vol 9 (2) ◽  
pp. 219-226 ◽  
Author(s):  
Caroline Brandi Schlaepfer-Sales ◽  
Ludmila De Faro Valverde ◽  
Rosane Borges Dias ◽  
Bruna Natália Alves Da Silva Pimentel ◽  
Leonardo De Araújo Melo ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Odontogenic Tumor ◽  
Keratocystic Odontogenic Tumor ◽  
Radiographic Features

scholarly journals Mandibular odontogenic sarcoma (ameloblastic fibrodentinosarcoma) in an aged cat — Short communication

2017 ◽  
Vol 65 (1) ◽  
pp. 89-95
Author(s):  
Gustavo A. Ramírez ◽  
Lorenzo Ressel ◽  
Jaume Altimira ◽  
Miquel Vilafranca
Keyword(s):  
Alveolar Bone ◽  
S100 Protein ◽  
Final Diagnosis ◽  
Proliferation Index ◽  
Cell Component ◽  
Ki 67 ◽  
Histologic Subtype ◽  
Odontogenic Tumours ◽  
Odontogenic Epithelium ◽  
Shaped Cell

A 13-year-old male cat presented with an ill-defined mass in the rostral mandible causing destruction and loss of alveolar bone. Microscopically, the mass consisted of cords or islands of benign odontogenic epithelium and a malignant, pleomorphic spindle-shaped cell component with dysplastic dentine formation. Immunohistochemically, neoplastic mesenchymal cells proved to be strongly positive for vimentin and negative for cytokeratins, desmin, actin and S100 protein; the Ki67 proliferation index was high. Morphological and immunohistochemical features largely overlap those reported for ameloblastic fibrodentinosarcoma, an uncommon histologic subtype of odontogenic sarcoma recognised in humans but no reported previously in animals. Ki-67 expression assessment may help to discriminate between malignant and benign forms of odontogenic tumours but the final diagnosis is mainly morphological.

scholarly journals Current Paradigm of Treatment for Pancreatic Neuroendocrine Tumor

2021 ◽  
Vol 26 (1) ◽  
pp. 24-32
Author(s):  
Min Je Sung ◽  
Moon Jae Chung
Keyword(s):  
Cell Proliferation ◽  
Medical Treatment ◽  
Neuroendocrine Tumor ◽  
Standard Treatment ◽  
Pancreatic Neuroendocrine Tumor ◽  
Somatostatin Analogues ◽  
Proliferation Index ◽  
Ki 67 ◽  
Grade 3 ◽  
Cell Proliferation Index

Pancreatic neuroendocrine tumor (PNET) refer to tumors originating from the islet of Langerhans and shows various prognosis based on the presence or absence of symptoms due to hormone secretion, the Ki-67 cell proliferation index, and the histologic grade, and according to the degree of disease progression defined by the tumor-node-metastasis (TNM) stage classification. The purpose of medical treatment for PNET is to control symptoms or inhibit tumor growth. Somatostatin analogues can be administered for the purpose of controlling symptoms caused by the secretion of specific hormones, and are accepted as effective drugs for inhibiting the progression of G1/G2 tumors based on World Health Organization (WHO) classification with a Ki-67 cell proliferation index less than 20%. Among the molecularly targeted agents, everolimus and sunitinib can be considered in patients with WHO G1/G2 PNET showing progression after somatostatin analog therapy. Cytotoxic chemotherapy is generally administered to patients with large tumor volume and rapidly progressing metastatic NET, and etoposide/cisplatin combination therapy has been considered as a standard treatment. For the patient group of Grade 3 PNET (well differentiated) newly classified by the WHO 2017 classification, guidelines for standard treatment have not yet been established. As it has been reported, studies are needed to evaluate the treatment response rate of somatostatin analogues or molecularly targeted therapies for the patient with Grade 3 PNET. It is important to consider a multidisciplinary approach with all possible treatment options including medical treatment, radical resection of primary or metastatic lesions, liver-directed therapies, and peptide receptor radionuclide therapy for the patients with PNET.

scholarly journals A Histopathological and Immunohistochemical Analysis of Ameloblastic Fibrodentinoma

2013 ◽  
Vol 2013 ◽  
pp. 1-7
Author(s):  
Ronell Bologna-Molina ◽  
Sirced Salazar-Rodríguez ◽  
Ana María Bedoya-Borella ◽  
Ramón Gil Carreón-Burciaga ◽  
Gabriel Tapia-Repetto ◽  
...  
Keyword(s):  
Histone H3 ◽  
Immunohistochemical Analysis ◽  
Odontogenic Tumor ◽  
Rare Entity ◽  
Ki 67 ◽  
Dental Papilla ◽  
Odontogenic Epithelium ◽  
Ameloblastic Fibroma ◽  
Large Panel

Ameloblastic fibrodentinoma (AFD) is considered a mixed odontogenic tumor that is characterized by conserved epithelial and ectomesenchymal neoplastic components. AFD is composed of long narrow cords and islands of odontogenic epithelium; the epithelial strands lie in a myxoid cell-rich ectomesenchymal tissue with stellate-shaped fibroblasts that exhibit long slender cytoplasmic extensions that resemble dental papilla. The lesions show the presence of dysplastic dentin. Although AFD is a rare entity and its very existence is not completely accepted, based on the extent of histodifferentiation, it is considered to represent a stage between ameloblastic fibroma and ameloblastic fibroodontoma. This study aimed to provide a histopathological and immunohistochemical characterization of this infrequent tumor. A large panel of antibodies including amelogenin, Ck 19, calretinin, syndecan-1, E-cadherin, MSH2, histone H3, and Ki-67 was used to illustrate the nature of the tumor.

scholarly journals The Possible Role of Mast Cells in the Odontogenic Cyst’s Pathogenesis: A Comparative Study between Dentigerous Cyst and Keratocystic Odontogenic Tumor

2016 ◽  
Vol 2016 ◽  
pp. 1-4 ◽  
Author(s):  
Sareh Farhadi ◽  
Fatemeh Shahsavari ◽  
MirMahdi Davardan
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Dentigerous Cyst ◽  
Test Method ◽  
Odontogenic Tumor ◽  
Blue Staining ◽  
Keratocystic Odontogenic Tumor ◽  
Wide Range ◽  
Significant Difference ◽  
Cell Densities

Background. Recently, mast cells were recognized in the pathogenesis of more aggressive pathologic lesions. This study was aimed to evaluate and compare the density of mast cells in Dentigerous cyst (DC) and Keratocystic odontogenic tumor (KCOT) regarding their different clinical behavior. Method. This study was conducted on 23 and 26 cases of DC and KCOT, respectively. Four-micron sections were prepared for Toluidine blue staining and mast cell densities in two desired cysts were studied. Final data was analyzed via t-test and Mann-Whitney U test method regarding the significant level lower than 0.05. Results. Mast cell densities were significantly higher in KCOTs for deep and superficial layers and both layers (P<0.05). The density of degranulated mast cells in the deeper layers and both layers was significantly higher in KCOTs (P<0.05). However, the density of degranulated mast cells in the superficial layer had no significant difference (P>0.05). Conclusion. It seems that mast cells may be involved in the pathogenesis of KCOT, but, regarding wide range of mast cell’s biologic activities, further investigations are recommended to confirm the issue and prepare the details.

Canine Neuroendocrine Tumors of the Pancreas: A Study Using Image Analysis Techniques for the Discrimination of Metastatic Versus Nonmetastatic Tumors

1997 ◽  
Vol 34 (2) ◽  
pp. 138-145 ◽  
Author(s):  
G. Minkus ◽  
U. Jütting ◽  
M. Aubele ◽  
K. Rodenacker ◽  
P. Gais ◽  
...  
Keyword(s):  
Image Analysis ◽  
Neuroendocrine Tumors ◽  
Nucleolar Organizer ◽  
Biological Behavior ◽  
Proliferation Index ◽  
Nucleolar Organizer Regions ◽  
Ki 67 ◽  
Analysis Techniques ◽  
Significant Difference ◽  
Image Analysis Techniques

Canine pancreatic neuroendocrine tumors were studied using different image analysis techniques (nuclear image histometry, analysis of argyrophilic proteins of nucleolar organizer regions, determination of the mouse anti-Ki 67 antigen proliferation index, and DNA densitometry) to correlate their biological behavior with objective phenotypic markers. The methods were compared to determine the best method for distinguishing between metastatic and nonmetastatic tumors. Discrimination between the two types of tumor was possible using nuclear image histometry in combination with morphometric analysis of argyrophilic proteins of nucleolar organizer regions. In contrast, the mouse anti-Ki 67 antigen proliferation index, DNA measurement, and immunohistochemical parameters revealed no significant difference between the two types of tumors.

scholarly journals A Keratocyst in the Buccal Mucosa with the Features of Keratocystic Odontogenic Tumor

2013 ◽  
Vol 7 (1) ◽  
pp. 152-156 ◽  
Author(s):  
Kazuhiko Yamamoto ◽  
Yumiko Matsusue ◽  
Miyako Kurihara ◽  
Yuka Takahashi ◽  
Tadaaki Kirita
Keyword(s):  
Buccal Mucosa ◽  
Basal Layer ◽  
Odontogenic Tumor ◽  
High Rate ◽  
High Signal ◽  
Keratocystic Odontogenic Tumor ◽  
Ki 67 ◽  
Weighted Image ◽  
Squamous Cells ◽  
The Right

A 74-year-old male patient consulted us for an elastic firm mass in the right buccal mucosa. CT examination revealed a well-circumscribed oval cystic lesion in the anterior region of the masseter muscle. On MRI, the lesion showed a low signal on T1-weighted image and a high signal on T2-weighted image. Aspiration biopsy demonstrated the presence of squamous cells in whitish liquid. Under the diagnosis of epidermoid cyst, the lesion was intraorally extirpated under general anesthesia. The lesion was cystic at the size of 30 × 25mm. Histologically, the cyst wall was lined with parakeratinized squamous epithelium corrugated on its surface, the basal layer of which consisted of cuboidal cells showing palisading of the nuclei. Immunohistochemically, the lining epithelium was positive for CK17 and negative for CK10. The basal and suprabasal cells were labeled for Ki-67 at a relatively high rate. These features are compatible with those of keratocystic odontogenic tumor.

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