scholarly journals FASandFASLGene Polymorphisms Are Not Associated with Hepatitis B Virus Infection Based on a Case-Control Study in a Brazilian Population

2013 ◽  
Vol 35 ◽  
pp. 741-746 ◽  
Author(s):  
Bárbara B. Santana ◽  
Maria Luana C. Viégas ◽  
Simone R. S. S. Conde ◽  
Marluísa O. G. Ishak ◽  
Ricardo Ishak ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Hbv Infection ◽  
Control Group ◽  
Active Chronic Hepatitis ◽  
Nucleotide Polymorphisms ◽  
Genotype Frequencies ◽  
Brazilian Amazon Region ◽  
B Virus

Objective. This study investigated the association of the single nucleotide polymorphisms (SNPs) in theFASandFASLgenes with the outcome of hepatitis B virus (HBV) infection.Methods. Blood samples were collected from 116 HBV-infected patients at the Hospital of the Santa Casa de Misericordia Foundation (Belém, PA, Brazil). Seronegative individuals were used as controls. DNA samples were extracted from the leukocytes and assayed using the polymerase chain reaction (PCR) followed by RFLP analysis with restriction endonucleases.Results. The frequencies of the mutant genotypes for -670FAS(GG), Ivs2nt-124FASL(GG), Ivs3nt-169FASL(ΔT/ΔT), and -844FASL(TT) were higher in the HBV patients, and theFAS-1377AA genotype was more frequent in the control group; however, the differences between the allele and genotype frequencies were not statistically significant. When the HBV patient population was divided into two groups (inactive carriers and active chronic hepatitis patients), the mutant genotypes were found to be more prevalent in the active chronic hepatitis group with respect to theFASgene polymorphisms; however, this difference was not statistically significant.Conclusions. The results suggest that the polymorphisms inFASandFASLgenes are not associated with HBV infection or even with the natural history of the infection in the Brazilian Amazon region.

scholarly journals IL-10 -1082 A>G (rs1800896) Polymorphism is Effective in Clearing Hepatitis B Virus Infection

2021 ◽  
Vol 14 (4) ◽  
Author(s):  
Neda Mahavar ◽  
Mohsen Naseri ◽  
Gholamreza Anani Sarab ◽  
Mohammad Fereidouni ◽  
Masood Ziaee ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Chronic Hepatitis B ◽  
Study Groups ◽  
Hbv Infection ◽  
Nucleotide Polymorphisms ◽  
Genetic Characteristics ◽  
B Virus ◽  
Reduced Risk

Background: Hepatitis B Virus (HBV) is a universal health challenge all around the world. Several factors like viral load, genetic characteristics, age, sex, and immune status contribute to variable clinical outcomes of HBV infection. The sequels of HBV infection vary remarkably among persons ranging from the spontaneous deletion of infection to persistent infection. Objective: The present study aimed to evaluate the association of single nucleotide polymorphisms IL10-1082 with HBV clearance. Methods: Sixty subjects with Chronic Hepatitis B (CHB) infection and 60 subjects who spontaneously recovered HBV were enrolled in the study. The IL-10-1082 polymorphisms were determined by Polymerase Chain Reaction with Restriction Fragment Length Polymorphism (PCR–RFLP). Results: The clearance of HBV infection demonstrated a significant association with IL-10-1082 polymorphisms in the GG genotype (P = 0.03), while there was no association with other genotypes. Reduced risk of chronic hepatitis B infection was associated with IL-10-1082 GG (OR: 2.33, 95% CI: 1.07 - 5.09). Besides, IL-10-1082 A/G alleles did not differ clearly between the two study groups (P = 0.07) Conclusions: The IL-10-1082 polymorphisms may be associated with a reduced risk of CHB infection and recovery after HBV infection.

scholarly journals NTCP gene polymorphisms and Hepatitis B virus infection status in a Ghanaian population

2020 ◽  
Author(s):  
Eric Nyarko ◽  
Christian Obirikorang ◽  
W.K.B.A. Owiredu ◽  
Evans Adu Asamoah ◽  
Emmanuel Acheampong ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Genetic Model ◽  
Additive Model ◽  
Minor Allele ◽  
Hbv Infection ◽  
Control Group ◽  
Case Group ◽  
Nucleotide Polymorphisms ◽  
B Virus

Abstract BackgroundSLC10A1 gene codes NTCP, a receptor through which the hepatitis B virus (HBV) gets access into hepatocytes - a stage of the viral cycle necessary for replication. Polymorphism variants of SLC10A1 play roles in HBV infection, viral clearance, treatment outcome, and complications, in diverse ethnic groups and countries. However, no such study has been conducted in the Ghanaian population, a country with HBV endemicity. Therefore, an exploratory study was conducted to investigate the presence of three (3) single nucleotide polymorphisms (SNPs) in the SLC10A1 gene (rs2296651, rs61745930, and rs4646287) and assessed the risk of HBV infection among the Ghanaian population.MethodPolymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to determine the presence of the SNPs among 292 participants comprising 146 HBV infected persons as case-subjects and 146 HBV non-infected persons as control-subjects. ResultsThe minor allele frequency (T) of rs2296651 was present in a significantly high proportion of cases compared with the control group (12.6% vs. 3.1%, p < 0.0001). The homozygote recessive variant of rs61745930 was present in 2.7% of the control group and 5.5% of the case group. Moreover, the minor allele frequencies of rs4646287 were 9.6% and 8.6% among the control and the case group, respectively (p = 0.767). Under the dominant (CC) genetic model of inheritance, rs2296651 was found to be protective of HBV infection [OR = 0.18 (0.07–0.44)], whereas under the co-dominant and additive model, rs2296651 was a potential risk factor for HBV infection [OR = 5.2 (95%CI: 2.1–12.8); 3.5 (95%CI: 1.6–7.6], respectively. Variants of rs61745930 and rs4646287 were not associated with HBV infection (p > 0.05). Polymorphisms in SLC10A1, however, did not show any significant association with HBV infectivity (p > 0.05).ConclusionThe study highlights some polymorphism proof that variants rs2296651, rs61745930, and rs4646287 exist in HBV-infected individuals in Ghana. Variant rs2296651 was found to be associated with HBV infection. Nonetheless, polymorphisms in SLC10A1 were not associated with HBV infectivity among the Ghanaian population. Further investigation is warranted to assess the offensive role of the relationship between rs2296651 and HBV infectivity.

scholarly journals Hepatitis B virus infection and ABO/Rh blood groups

2017 ◽  
Vol 5 (9) ◽  
pp. 3782
Author(s):  
Ozlem Genc
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Blood Group ◽  
Blood Groups ◽  
Hbv Infection ◽  
Control Group ◽  
Chronic Hbv Infection ◽  
B Virus ◽  
Chronic Hbv

Background: Chronic HBV infection is a significant public health problem all around the world. It is not clear if ABO/Rh blood groups have a role in the development of chronic forms of hepatitis B virus (HBV) infection. The aim of the study was to investigate the relationship between chronic HBV infection and ABO/Rh blood groups.Methods: The study was designed as a retrospective study that included totaled 937 individuals: 453 patients diagnosed with chronic hepatitis and 484 healthy individuals as the control group. HBsAg (hepatitis B surface antigen) and blood groups results of patients between the years 2013-2015 were collected by reviewing the laboratory results. During these three years HBsAg was performed on the architect i2000sr (Abbott diagnostic, Illinois, USA) with ELISA and ABO/Rh blood groups were analyzed with gel centrifugation method (Grifols, Barcelona, Spain).Results: Blood group A Rh positive was higher than other blood types in the chronic hepatitis B group (44.3%) and in control group (41.9%), whereas blood types O, AB, and B were similar between cases with chronic hepatitis and controls (p>0.05). HBV infection was moderately less frequent in subjects with AB positive blood group (p=0.07). The prevalence of Rh positive was 89.1% in patients and 88.2% in the control group (p>0.05).Conclusions: This study showed that there was not an association between ABO/Rh blood groups and chronic HBV infection, but there is a need for different and more numerous case-control studies about this subject.

scholarly journals NTCP gene polymorphisms and Hepatitis B virus infection status in a Ghanaian population

2020 ◽  
Author(s):  
Eric Nyarko ◽  
Christian Obirikorang ◽  
W.K.B.A. Owiredu ◽  
Evans Adu Asamoah ◽  
Emmanuel Acheampong ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Genetic Model ◽  
Additive Model ◽  
Minor Allele ◽  
Hbv Infection ◽  
Control Group ◽  
Case Group ◽  
Nucleotide Polymorphisms ◽  
B Virus

Abstract Background: SLC10A1 gene codes NTCP, a receptor through which the hepatitis B virus (HBV) gets access into hepatocytes - a stage of the viral cycle necessary for replication. Polymorphism variants of SLC10A1 play roles in HBV infection, viral clearance, treatment outcome, and complications, in diverse ethnic groups and countries. However, no such study has been conducted in the Ghanaian population, a country with HBV endemicity. Therefore, an exploratory study was conducted to investigate the presence of three (3) single nucleotide polymorphisms (SNPs) in the SLC10A1 gene (rs2296651, rs61745930, and rs4646287) and assessed the risk of HBV infection among the Ghanaian population. Method: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to determine the presence of the SNPs among 292 participants comprising 146 HBV infected persons as case-subjects and 146 HBV non-infected persons as control-subjects. Results: The minor allele frequency (T) of rs2296651 was present in a significantly high proportion of cases compared with the control group (11.6% vs. 3.1%, p<0.0001). The homozygote recessive variant of rs61745930 was present in 2.7% of the control group and 5.5% of the case group. Moreover, the minor allele frequencies of rs4646287 were 9.3% and 8.2% among the control and the case group, respectively (p =0.767). Under the dominant (CC) genetic model of inheritance, rs2296651 was found to be protective of HBV infection [OR= 0.18 (0.07-0.44)], whereas under the co-dominant and additive model, rs2296651 was a potential risk factor for HBV infection [OR= 5.2 (95%CI: 2.1 -12.8); 3.5 (95%CI: 1.6 -7.6], respectively. Variants of rs61745930 and rs4646287 were not associated with HBV infection (p > 0.05). Polymorphisms in SLC10A1, however, did not show any significant association with HBV infectivity (p >0.05). Conclusion: The study highlights some polymorphism proof that variants rs2296651, rs61745930, and rs4646287 exist in HBV-infected individuals in Ghana. Although variant rs2296651 was found to be associated with HBV infection, this association warrants more studies. Polymorphisms in SLC10A1 were not associated with HBV infectivity among the Ghanaian population. Further investigation is warranted to assess the offensive role of the relationship between rs2296651 and HBV infectivity.

High prevalence of occult hepatitis C virus infection in patients with chronic hepatitis B virus infection

2013 ◽  
Vol 62 (8) ◽  
pp. 1235-1238 ◽  
Author(s):  
Inmaculada Castillo ◽  
Javier Bartolomé ◽  
Juan Antonio Quiroga ◽  
Vicente Carreño
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Hepatitis B Virus ◽  
Virus Infection ◽  
Hepatitis B ◽  
Hcv Infection ◽  
Hbv Infection ◽  
Chronic Hepatitis B Virus ◽  
B Virus

Hepatitis C virus (HCV) infection in the absence of detectable antibodies against HCV and of viral RNA in serum is called occult HCV infection. Its prevalence and clinical significance in chronic hepatitis B virus (HBV) infection is unknown. HCV RNA was tested for in the liver samples of 52 patients with chronic HBV infection and 21 (40 %) of them were positive for viral RNA (occult HCV infection). Liver fibrosis was found more frequently and the fibrosis score was significantly higher in patients with occult HCV than in negative ones, suggesting that occult HCV infection may have an impact on the clinical course of HBV infection.

scholarly journals Cytokine profile during occult hepatitis B virus infection in chronic hepatitis C patients

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Camilla Rodrigues de Almeida Ribeiro ◽  
Nathalia Alves Araújo de Almeida ◽  
Katrini Guidolini Martinelli ◽  
Marcia Amendola Pires ◽  
Carlos Eduardo Brandao Mello ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis C ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Chronic Hepatitis C ◽  
Control Group ◽  
Occult Hepatitis ◽  
Tnf Α ◽  
B Virus ◽  
Occult Hepatitis B

Abstract Background The hepatitis B virus (HBV) is one of the leading causes of acute, chronic and occult hepatitis (OBI) representing a serious public health threat. Cytokines are known to be important chemical mediators that regulate the differentiation, proliferation and function of immune cells. Accumulating evidence indicate that the inadequate immune responses are responsible for HBV persistency. The aim of this study were to investigate the cytokines IFN-γ, TNF-α, IL-2, IL-4, IL-6, IL-10 and IL-17A in patients with OBI and verify if there is an association between the levels of these cytokines with the determination of clinical courses during HBV occult infection. Methods 114 patients with chronic hepatitis C were investigated through serological and molecular tests, the OBI coinfected patients were subjected to the test for cytokines using the commercial human CBA kit. As controls, ten healthy donors with no history of liver disease and 10 chronic HBV monoinfected patients of similar age to OBI patients were selected. Results Among 114 HCV patients investigated, 11 individuals had occult hepatitis B. The levels of cytokines were heterogeneous between the groups, most of the cytokines showed higher levels of production detection among OBI/HCV individuals when compared to control group and HBV monoinfected pacients. We found a high level of IL-17A in the HBV monoinfected group, high levels of TNF-α, IL-10, IL-6, IL-4 and IL-2 in OBI/HCV patients. Conclusion These cytokines could be involved in the persistence of HBV DNA in hepatocytes triggers a constant immune response, inducing continuous liver inflammation, which can accelerate liver damage and favor the development of liver cirrhosis in other chronic liver diseases.

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