scholarly journals Leptin Level and Oxidative Stress Contribute to Obesity-Induced Low Testosterone in Murine Testicular Tissue

2014 ◽  
Vol 2014 ◽  
pp. 1-14 ◽  
Author(s):  
Jian Zhao ◽  
Lingling Zhai ◽  
Zheng Liu ◽  
Shuang Wu ◽  
Liping Xu
Keyword(s):  
Oxidative Stress ◽  
High Fat Diet ◽  
Leydig Cells ◽  
Sperm Count ◽  
Reproductive Organs ◽  
Testis Weight ◽  
Diet Induced Obesity ◽  
Short And Long Term ◽  
Testis Tissue

Objective. This study evaluated the effects of obesity on the function of reproductive organs in male mice and the possible mechanism of male secondary hypogonadism (SH) in obesity.Methods. Ninety-six mice were randomly assigned to three groups: the control group, diet-induced obesity group, and diet-induced obesity resistant group for 8 weeks and 19 weeks. The effects of short- and long-term high-fat diet on the reproductive organs were determined by measuring sperm count and motility, relative testis weight, testosterone level, pathological changes and apoptosis of Leydig cells. Oxidative stress was evaluated by determining malondialdehyde, H2O2, NO levels, and GSH in testis tissues. CAT, SOD, GSH-Px and Nrf2mRNA were measured by real-time PCR.Results. Short- and long-term high-fat diet decreased sperm count and motility, relative testis weight, testosterone level; decreased CAT, SOD, GSH-Px and Nrf2mRNA expression; increased MDA, H2O2, NO and leptin levels; inhibited the activity of CAT and GSH-Px enzymes. Pathological injury and apoptosis of Leydig cells were found in testis tissue.Conclusions. Pathological damage of Leydig cells, oxidative stress in testis tissue, and high level of leptin may provide some evidence to clarify the mechanisms of male SH in obesity.

scholarly journals Spermatotoxic Effects of Single-Walled and Multi-Walled Carbon Nanotubes on Male Mice

2020 ◽  
Vol 7 ◽  
Author(s):  
Omid Farshad ◽  
Reza Heidari ◽  
Mohammad Javad Zamiri ◽  
Socorro Retana-Márquez ◽  
Meghdad Khalili ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Carbon Nanotubes ◽  
Sperm Count ◽  
Weight Coefficient ◽  
Reproductive Organs ◽  
Significant Decrement ◽  
Total Antioxidant ◽  
Multi Walled Carbon Nanotubes ◽  
Carbon Based Nanomaterials ◽  
Walled Carbon Nanotubes

Carbon-based nanomaterials possess a remarkably high potential for biomedical applications due to their physical properties; however, their detrimental effects on reproduction are also concerned. Several reports indicate the toxicity of carbon nanotubes (CNT); nevertheless, their impact on intracellular organelles in the male reproductive organs has not been fully elucidated. Herein, we report on the reprotoxicity of single-walled (SWCNT) and multi-walled carbon nanotubes (MWCN) on several intracellular events and histological criteria in pubertal male BALB/c mice orally treated with 0, 10, and 50 mg/kg/day doses for 5 weeks. Biomarkers of oxidative stress and mitochondrial functionality, histopathological alterations, and epididymal sperm characteristics were determined. Oral administration of CNTs at 10 and 50 mg/kg evoked a significant decrement in weight coefficient, sperm viability and motility, hypo-osmotic swelling (HOS) test, sperm count, mitochondrial dehydrogenase activity, ATP content, total antioxidant capacity, and GSH/GSSH ratio in the testis and epididymal spermatozoa. On the other hand, percent abnormal sperm, testicular and sperm TBARS contents, protein carbonylation, ROS formation, oxidized glutathione level, and sperm mitochondrial depolarization were considerably increased. Significant histopathological and stereological alterations in the testis occurred in the groups challenged with CNTs. The current findings indicated that oxidative stress and mitochondrial impairment might substantially impact CNTs-induced reproductive system injury and sperm toxicity. The results can also be used to establish environmental standards for CNT consumption by mammals, produce new chemicals for controlling the rodent populations, and develop therapeutic approaches against CNTs-associated reproductive anomalies in the males exposed daily to these nanoparticles.

scholarly journals Nrf2 Represses FGF21 During Long-Term High-Fat Diet-Induced Obesity in Mice

Diabetes ◽  
2011 ◽  
Vol 60 (10) ◽  
pp. 2465-2473 ◽  
Author(s):  
D. V. Chartoumpekis ◽  
P. G. Ziros ◽  
A. I. Psyrogiannis ◽  
A. G. Papavassiliou ◽  
V. E. Kyriazopoulou ◽  
...  
Keyword(s):  
High Fat Diet ◽  
High Fat ◽  
Diet Induced Obesity ◽  
Obesity In Mice

Short- and long-term alterations of mitochondrial morphology, dynamics and mtDNA after transient oxidative stress

Mitochondrion ◽  
2008 ◽  
Vol 8 (4) ◽  
pp. 293-304 ◽  
Author(s):  
Marina Jendrach ◽  
Sören Mai ◽  
Sandra Pohl ◽  
Monika Vöth ◽  
Jürgen Bereiter-Hahn
Keyword(s):  
Oxidative Stress ◽  
Mitochondrial Morphology ◽  
Short And Long Term

A long-term high-fat diet increases oxidative stress, mitochondrial damage and apoptosis in the inner ear of d-galactose-induced aging rats

2012 ◽  
Vol 287 (1-2) ◽  
pp. 15-24 ◽  
Author(s):  
Zhengde Du ◽  
Yang Yang ◽  
Yujuan Hu ◽  
Yu Sun ◽  
Sulin Zhang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Inner Ear ◽  
High Fat Diet ◽  
Mitochondrial Damage ◽  
High Fat ◽  
Aging Rats

Differential effects of short- and long-term high-fat diet feeding on hepatic fatty acid metabolism in rats

2011 ◽  
Vol 1811 (7-8) ◽  
pp. 441-451 ◽  
Author(s):  
Jolita Ciapaite ◽  
Nicole M. van den Broek ◽  
Heleen te Brinke ◽  
Klaas Nicolay ◽  
Jeroen A. Jeneson ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Metabolism ◽  
High Fat Diet ◽  
Acid Metabolism ◽  
High Fat ◽  
Differential Effects ◽  
Hepatic Fatty Acid ◽  
Short And Long Term

scholarly journals Long-term high-fat diet-induced obesity decreases the cardiac leptin receptor without apparent lipotoxicity

Life Sciences ◽  
2011 ◽  
Vol 88 (23-24) ◽  
pp. 1031-1038 ◽  
Author(s):  
André F. Nascimento ◽  
Renata A.M. Luvizotto ◽  
André S. Leopoldo ◽  
Ana P. Lima-Leopoldo ◽  
Fábio R. Seiva ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Leptin Receptor ◽  
High Fat ◽  
Diet Induced Obesity
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