scholarly journals Prognostic Efficacy of Nuclear Morphometry at Invasive Front of Oral Squamous Cell Carcinoma: An Image Analysis Microscopic Study

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
V. K. Vaishnavi Vedam ◽  
Karen Boaz ◽  
Srikant Natarajan
Keyword(s):  
Squamous Cell Carcinoma ◽  
Image Analysis ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Tnm Staging ◽  
Molecular Characteristics ◽  
Grading System ◽  
Invasive Front ◽  
Nuclear Morphometry

Background. Oral cancer is revisited on a pathologist perspective with advanced imaging technique.Objective. The present study assessed the new malignancy grading system at tumor proper (TP) and Bryne’s grading system at invasive tumor front (ITF), morphometric features using software, to clarify their associations with prognosis of oral cancers.Methods. Histologically confirmed oral squamous cell carcinoma (OSCC) with 5-year follow-up was assessed morphometrically using image analysis at TP and ITF, correlated with the prognosis of patient.Results. On comparison of grading systems, a moderate agreement between both (Bryne and Anneroth) was seen. Among all histological parameters, we noted inverse correlation between degree of mitosis at invasive front and decrease in lymphoplasmacytic infiltrate at ITF with increase rate of recurrence and event of death. An increase in nuclear area, diameter, and perimeter along with decrease circularity in advancing OSCC was seen. Correlation of parameters showed higher values for maximum nuclear diameter, perimeter, and circularity at TP and ITF with recurrence.Conclusion. This study, while limited in sample size, concluded that a combined assessment of clinical TNM staging, histopathological grading system{excluding the parameter “mitotic activity” (due to its inverse relation)}, and nuclear morphometry at the ITF are better prognosticators. This combination proved to be an accurate predictive factor in eliciting the varied molecular characteristics of tumor heterogeneity.

scholarly journals Tumor budding and depth of invasion can be used as prognostic risk factors in determining treatment plan for early stage oral squamous cell carcinoma

2019 ◽  
Vol 7 (10) ◽  
pp. 3854
Author(s):  
Kaduganoor Ramakrishnan Mohan ◽  
Rahamathulla Mudassar Sharief ◽  
Rahila C.
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Early Stage ◽  
Treatment Plan ◽  
Tumor Budding ◽  
Grading System ◽  
Depth Of Invasion ◽  
Invasive Front

Background: The grading of oral squamous cell carcinoma can be useful along with TNM staging in determining treatment plan. The aim is to evaluate the prognostic value of histopathological grading of oral squamous cell carcinoma and to find its importance in setting appropriate treatment plan.Methods: The study includes 60 oral squamous cell carcinoma cases surgically operated during January 2012 to December 2018. From the archival paraffin blocks and available resected specimens of each case, the histological parameters used in Bryne’s invasive grading system and Almangush BD model were evaluated and compared to their prognosis.Results: The parameters used in BD model-tumor budding and depth of invasion were found to be statistically significant with prognosis of the disease. Except for nuclear polymorphism, the parameters used in Bryne’s invasive front grading system do not correlate with prognosis.Conclusion: Based on the prognostic significance, tumor budding ≥5 buds in the invasive front area and depth of invasion ≥4mm can be used as risk factors in prospective clinical trials by considering them in early stage disparity cases for multimodality treatment approach and elective neck dissection.

Clinicobiological progression and prognosis of oral squamous cell carcinoma in relation to the tumor invasive front: impact on prognosis

2014 ◽  
Vol 134 (4) ◽  
pp. 416-424 ◽  
Author(s):  
Kishore Sandu ◽  
Lluís Nisa ◽  
Philippe Monnier ◽  
Christian Simon ◽  
Snezana Andrejevic-Blant ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Invasive Front ◽  
Front Impact

Basal Ki67 expression measured by digital image analysis is optimal for prognostication in oral squamous cell carcinoma

2012 ◽  
Vol 48 (14) ◽  
pp. 2166-2174 ◽  
Author(s):  
Alexander C. Klimowicz ◽  
Pinaki Bose ◽  
Steven C. Nakoneshny ◽  
Michelle Dean ◽  
Longlong Huang ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Image Analysis ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Digital Image ◽  
Digital Image Analysis ◽  
Ki67 Expression

Association between central and peripheral circulating tumor cell (CTC) clusters in oral squamous cell carcinoma (OSCC): A prospective, observational study.

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e15548-e15548
Author(s):  
Ritvi K Bagadia ◽  
Vishal Uchila Shishir Rao ◽  
Ajay Balakrishnan ◽  
Abhijith George ◽  
Prashant Kumar
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Observational Study ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Early Stage ◽  
Clinical Stage ◽  
Survival Rates ◽  
Tnm Staging ◽  
Dapi Staining

e15548 Background: Around 90% of cancer-related mortalities are caused by tumor metastasis. CTC clusters, which constitute an intermediate stage of metastasis, have not been studied extensively in head & neck cancers. The mortality rate of oral cancers remains alarmingly high, despite multimodality treatment. The aim of the study is to identify the presence of CTC clusters in patients with Oral Squamous Cell Carcinoma (OSCC) and to correlate their presence with clinical and pathological factors. Methods: Fifty patients diagnosed with histologically proven OSCC, treatment naïve, and underwent surgery at HCG Cancer Centre, Bangalore, were consented and enrolled in the study. An IRB-approved protocol allowed for the collection of 10 ml of blood from central (jugular) and peripheral veins intra-operatively, prior to tumor removal. The culturing of CTC clusters was done using ellipsoidal microwell plates maintained at hypoxic conditions, at the Institute of Bioinformatics, Bangalore. After fourteen days of culturing, the cells were fixed and stained for DAPI, Pan-CK and CD45. The CTC clusters were classified into Loose, Tight and very Tight based on the median gray values obtained from DAPI staining on ImageJ software. Clinical data was collected from patient records and subjected to analysis using Descriptive statistics. Results: From the 50 patients included in the study, 22 (44%) patients exhibited tight clusters in central blood, while only 13 (26%) patients exhibited tight clusters in peripheral blood. A higher clinical stage was observed in a greater percentage of patients with tight clusters in central blood (early: 45.5% versus late: 54.5%), but the same findings could not be inferred with pathological staging (early stage: 59.1% versus late stage: 40.1%). No significant correlation with adverse pathological features was noted. Conclusions: This observational study provides an insight into the varying biological behaviours of similarly grouped cancers, which is based on the standard TNM staging. The study forms the basis for the hypothesis of tight clusters in the central and peripheral circulation, correlating with loco-regional and distant metastasis respectively, thus leading to poorer disease-free and overall survival rates.

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