scholarly journals Overexpression of a Vesicle Trafficking Gene, OsRab7, Enhances Salt Tolerance in Rice

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Xiaojue Peng ◽  
Xia Ding ◽  
Tianfang Chang ◽  
Zhoulong Wang ◽  
Rong Liu ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Transgenic Rice ◽  
Agricultural Production ◽  
Vesicle Trafficking ◽  
Yeast Cells ◽  
Root Tip ◽  
Over Expression ◽  
Crop Tolerance ◽  
Vacuolar Trafficking ◽  
Main Factor

High soils salinity is a main factor affecting agricultural production. Studying the function of salt-tolerance-related genes is essential to enhance crop tolerance to stress.Rab7is a small GTP-binding protein that is distributed widely among eukaryotes. Endocytic trafficking mediated byRab7plays an important role in animal and yeast cells, but the current understanding ofRab7in plants is still very limited. Herein, we isolated a vesicle trafficking gene,OsRab7, from rice. Transgenic rice over-expressingOsRab7exhibited enhanced seedling growth and increased proline content under salt-treated conditions. Moreover, an increased number of vesicles was observed in the root tip ofOsRab7transgenic rice. TheOsRab7over-expression plants showed enhanced tolerance to salt stress, suggesting that vacuolar trafficking is important for salt tolerance in plants.

Over-expression of OSRIP18 increases drought and salt tolerance in transgenic rice plants

2011 ◽  
Vol 21 (4) ◽  
pp. 785-795 ◽  
Author(s):  
Shu-Ye Jiang ◽  
Ritu Bhalla ◽  
Rengasamy Ramamoorthy ◽  
Hong-Fen Luan ◽  
Prasanna Nori Venkatesh ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Transgenic Rice ◽  
Over Expression ◽  
Rice Plants ◽  
Transgenic Rice Plants ◽  
Drought And Salt Tolerance

Salt tolerance of transgenic rice overexpressing yeast mitochondrial Mn-SOD in chloroplasts

Plant Science ◽  
1999 ◽  
Vol 148 (2) ◽  
pp. 131-138 ◽  
Author(s):  
Y Tanaka ◽  
T Hibino ◽  
Y Hayashi ◽  
A Tanaka ◽  
S Kishitani ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Transgenic Rice

scholarly journals Verteporfin can Reverse the Paclitaxel Resistance Induced by YAP Over-Expression in HCT-8/T Cells without Photoactivation through Inhibiting YAP Expression

2016 ◽  
Vol 39 (2) ◽  
pp. 481-490 ◽  
Author(s):  
Wang Pan ◽  
Qian Wang ◽  
Yi Zhang ◽  
Naishu Zhang ◽  
Jiamin Qin ◽  
...  
Keyword(s):  
T Cells ◽  
Combination Therapy ◽  
Multiple Drug Resistance ◽  
Protein Detection ◽  
Multiple Drug ◽  
Over Expression ◽  
Anti Cancer ◽  
The Relationship ◽  
Proliferation And Invasion ◽  
Main Factor

Background/Aims: Paclitaxel (PTX) is one of the most effective anti-cancer drugs. However, multiple drug resistance is still the main factor that hinders the effective treatment of cancer with PTX. Several factors including YAP over-expression can cause PTX resistance. In this study, we aimed to verify the role YAP plays in PTX resistance, explore the reversal of PTX resistance by verteporfin (VP) and investigate the effect of combination therapy of PTX and VP on the PTX resistant colon cancer cells (HCT-8/T). Methods: To study the relationship between YAP and PTX resistance, a stable YAP-over-expression or YAP silencing cell line was generated by transfected with YAP-plasmids or siYAP-RNA. WST-1 assay was performed to detect the cytotoxicity of PTX on HCT-8 and HCT-8/T cells. Clone formation assay and Transwell assay was preformed to determine the cell proliferation and invasion ability respectively. Immunofluorescence and Western blot analysis was performed for protein detection. Results: YAP was stronger expressed in HCT-8/T than in HCT-8, and PTX resistance was positively correlated with the level of YAP expression. VP, a strongly YAP inhibitor, could reduce the PTX resistance on HCT-8/T cells without light activation by inhibiting YAP. Beside, VP and PTX combination therapy showed synergism on inhibition of YAP and cytotoxicity to HCT-8/T. Moreover, verteporfin and PTX combination therapy affect the invasion and colony formation ability and induce apoptosis of HCT-8/T cells. Conclusions: VP can reverse the PTX resistance induced by YAP over-expression in HCT-8/T cells without photoactivation through inhibiting YAP expression.

scholarly journals The Jacalin-Related Lectin HvHorcH Is Involved in the Physiological Response of Barley Roots to Salt Stress

2021 ◽  
Vol 22 (19) ◽  
pp. 10248
Author(s):  
Katja Witzel ◽  
Andrea Matros ◽  
Uwe Bertsch ◽  
Tariq Aftab ◽  
Twan Rutten ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Extracellular Fluid ◽  
Root Tip ◽  
Salinity Treatment ◽  
Root Tips ◽  
Salt Stress Response ◽  
Cdna Sequences ◽  
Barley Cultivars ◽  
Protein Levels

Salt stress tolerance of crop plants is a trait with increasing value for future food production. In an attempt to identify proteins that participate in the salt stress response of barley, we have used a cDNA library from salt-stressed seedling roots of the relatively salt-stress-tolerant cv. Morex for the transfection of a salt-stress-sensitive yeast strain (Saccharomyces cerevisiae YSH818 Δhog1 mutant). From the retrieved cDNA sequences conferring salt tolerance to the yeast mutant, eleven contained the coding sequence of a jacalin-related lectin (JRL) that shows homology to the previously identified JRL horcolin from barley coleoptiles that we therefore named the gene HvHorcH. The detection of HvHorcH protein in root extracellular fluid suggests a secretion under stress conditions. Furthermore, HvHorcH exhibited specificity towards mannose. Protein abundance of HvHorcH in roots of salt-sensitive or salt-tolerant barley cultivars were not trait-specific to salinity treatment, but protein levels increased in response to the treatment, particularly in the root tip. Expression of HvHorcH in Arabidopsis thaliana root tips increased salt tolerance. Hence, we conclude that this protein is involved in the adaptation of plants to salinity.

Over-expression of a plasma membrane H+-ATPase SpAHA1 conferred salt tolerance to transgenic Arabidopsis

PROTOPLASMA ◽  
2018 ◽  
Vol 255 (6) ◽  
pp. 1827-1837 ◽  
Author(s):  
Yafei Fan ◽  
Shumin Wan ◽  
Yingshuo Jiang ◽  
Youquan Xia ◽  
Xiaohui Chen ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Salt Tolerance ◽  
Transgenic Arabidopsis ◽  
Over Expression

scholarly journals Micro-economic analysis of firms differentiation

2012 ◽  
Vol 49 (No. 5) ◽  
pp. 217-224
Author(s):  
P. Bielik ◽  
Ľ. Gurčík ◽  
M. Rajčániová
Keyword(s):  
Performance Evaluation ◽  
Economic Analysis ◽  
Agricultural Production ◽  
Business Performance ◽  
Methodological Approach ◽  
Financial Indicators ◽  
Value Evaluation ◽  
Reform Period ◽  
Main Factor

After liberalisation established new relations in Slovak economy, it seemed that problems of agricultural companies differentiation would disappear. But the economic results of our companies confirm the existence of this problem. In the pre-reform period, agricultural production intensity was considered as a main factor of economic differentiation. Transformation of economy after 1990 changed the methodological approach to business performance evaluation. The interest was shifted towards the value evaluation comparing businesses with regard to financial indicators. These methods enable to classify enterprises into bonity classes and to set their sequence according to performance.

scholarly journals Glabridin triggers over-expression of apoptosis inducing factor (AIF) gene in Candida albicans

2018 ◽  
Author(s):  
Maryam Moazeni ◽  
Mohammad Taghi Hedayati ◽  
Mojtaba Nabili
Keyword(s):  
Candida Albicans ◽  
Real Time ◽  
Dna Cleavage ◽  
Wide Spectrum ◽  
Yeast Cells ◽  
Root Extract ◽  
Over Expression ◽  
Human Fungal Pathogen ◽  
Polymerase Chain ◽  
Apoptosis Inducing Factor

  Background and Purpose: Candida albicans is a prevalent human fungal pathogen that can cause a wide spectrum of diseases, from superficial mucosal infections to systemic disorders, in patients with impaired immunity. Glabridin is a pyranoisoflavan originally extracted from root extract of Glycyrrhiza glabra. Glabridin can also mediate apoptosis in yeast cells by changing the mitochondrial membrane potential, activation of caspase-like proteases, and DNA cleavage. The aim of this study was to investigate the mechanism of action of glabridin in C. albicans. Materials and Methods: Candida albicans ATCC14053 was applied as the standard strain. Total RNA was extracted from the isolate under glabridin-treated and untreated conditions. To evaluate the alternations in the apoptosis inducing factor (AIF) gene expression, real-time polymerase chain reaction (real-time -PCR) was performed, and the obtained data were analyzed using REST software. Results: Expression of the AIF gene was represented as the ratio of expression relative to the reference gene. According to the REST® output, the expression of the AIF gene increased significantly (P<0.05) under the glabridin-treated condition. Conclusion: Our results suggested that glabridin may induce apoptosis through the caspase-independent route and might be considered as an anti-Candida agent.

scholarly journals TaSRG , a wheat transcription factor, significantly affects salt tolerance in transgenic rice and Arabidopsis

FEBS Letters ◽  
2011 ◽  
Vol 585 (8) ◽  
pp. 1231-1237 ◽  
Author(s):  
Xiaoliang He ◽  
Xiaona Hou ◽  
Yinzhu Shen ◽  
Zhanjing Huang
Keyword(s):  
Transcription Factor ◽  
Salt Tolerance ◽  
Transgenic Rice
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