scholarly journals Quantification of Gymnemagenin and β-Sitosterol in Marketed Herbal Formulation by Validated Normal Phase HPTLC Method

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Sachin E. Potawale ◽  
Satish Y. Gabhe ◽  
Kakasaheb R. Mahadik
Keyword(s):  
High Performance ◽  
Research Study ◽  
Retention Factor ◽  
Solvent System ◽  
Polyherbal Formulation ◽  
Herbal Formulation ◽  
Acid Reagent ◽  
Development And Validation ◽  
Hptlc Method

This research study describes development and validation of new, rapid, accurate, robust, and precise, high performance thin layer chromatographic (HPTLC) method for concurrent quantitative determination of gymnemagenin and β-sitosterol in herbal formulation with densitometric detection. Chromatographic separation was achieved on Merck aluminum HPTLC plates precoated with silica gel 60 F254. The optimized solvent system consisted of toluene : ethyl acetate : methanol (6.5 : 2.5 : 1.4, v/v/v). Developed plates were derivatized with 5% sulphuric acid reagent followed by heating at 110°C for 4 min in a preheated oven and scanned at 423 nm in reflectance-absorbance mode. The retention factor for gymnemagenin and β-sitosterol was found to be 0.27±0.02 and 0.78±0.02, respectively. The proposed densitometric method was validated according to ICH Q2 (R1) guidelines. Results were found to be linear over a range of 100–1200 ng band−1 and 200–1200 ng band−1 for gymnemagenin and β-sitosterol, respectively. The percent content of gymnemagenin and β-sitosterol in the marketed polyherbal formulation was found to be 0.0405% and 0.1377%, respectively. The proposed HPTLC method can be used for quantification of gymnemagenin and β-sitosterol in marketed polyherbal formulation used in the study in quality-control laboratories.

DEVELOPMENT AND VALIDATION OF HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF RUTIN FROM THE AERIAL PARTS OF ALHAGI PSEUDALHAGI (M. BIEB) DESV

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (05) ◽  
pp. 37-41
Author(s):  
S. B Mishra ◽  
◽  
S Singh ◽  
A. Mukerjee
Keyword(s):  
Mobile Phase ◽  
High Performance ◽  
Chromatographic Method ◽  
Retention Factor ◽  
Aerial Parts ◽  
Ich Guidelines ◽  
Acid Reagent ◽  
Development And Validation ◽  
Hptlc Method

A simple, accurate and precise HPTLC method has been developed for the estimation of rutin from the aerial parts of Alhagi pseudalhagi (M. Bieb) Desv. The method employed silica gel 60 F254 precoated plates as stationary phase and a mixture of ethyl acetate: acetic acid: formic acid: water (10:1:1:1 v/v/v/v) as mobile phase. Densitometric scanning was performed at 366 nm after derivatizing the resolved peaks with anisaldehyde-sulphuric acid reagent. Beer’s law was obeyed in the concentration range of 400-1200ng/spot with regression equation for standard rutin as Y= 7.658x - 53.86 and r2=0.9885. The Retention factor for rutin is 0.39 ± 0.008. The % RSD (n=9) for interday, intraday precision and the recovery values were found to be 1.31% and 1.46% and 99.55-101.12%, respectively. The method was validated as per ICH Guidelines, proving its utility in estimation of rutin from the aerial parts of Alhagi pseudalhagi (M. Bieb) Desv.

scholarly journals DEVELOPMENT AND VALIDATION OF A HPTLC METHOD FOR SIMULTANEOUS DENSITOMETRIC ANALYSIS OF GLYCYRRHETIC ACID AND SOLASODINE IN HERBAL DRUG FORMULATION

2017 ◽  
Vol 9 (4) ◽  
pp. 29 ◽  
Author(s):  
Kinjal Patel ◽  
Chaitanya Bhatt
Keyword(s):  
High Performance ◽  
Area Under The Curve ◽  
Retention Factor ◽  
Drug Formulation ◽  
Glycyrrhetic Acid ◽  
Densitometric Analysis ◽  
Polyherbal Formulation ◽  
Quality Control Tool ◽  
Development And Validation ◽  
Hptlc Method

Objective: To develop and validate a simple, precise, selective, and accurate high-performance thin layer chromatographic method for simultaneous densitometric analysis of glycyrrhetic acid and solasodine in the polyherbal formulation.Methods: The method was developed using HPTLC silica gel GF254precoated aluminium plate as the stationary phase and Chloroform: Methanol (9:1 v/v) as the mobile phase. Quantization of glycyrrhetic acid was achieved by determining the area under the curve at 267 nm using CAMAG TLC Scanner and CATS 3 software. Since the structure of solasodine lacks conjugated double bond, it does not give any fluorescence either in the absorbance mode or reflectance mode hence solasodine was derivatized using 0.5%anisaldehyde sulphuric acids which gave a bluish spot as seen on TLC plate. These spots were scanned at 546 nm wavelength using CAMAG TLC Scanner and CATS 3 software.Results: The retention factor for glycyrrhetic acid and solasodine were found to be 0.52±0.01, 0.40±0.01% w/w respectively. The developed HPTLC method was validated using parameters described in International Conference on Harmonization (ICH) guideline. The proposed method showed good linearity in the range of 400-2000 ng spot-1 for glycyrrhetic acid as well as for solasodine. The content of glycyrrhetic acid and solasodine in marketed polyherbal formulation were found to be 0.67%±0.8 and 0.10±0.35%w/w respectively.Conclusion: The developed method can be used as quality control tool for the routine analysis of glycyrrhetic acid and solasodine in polyherbal formulation.

scholarly journals Development and validation of HPTLC method for the analysis of Olmutinib in Bulk drug

2020 ◽  
Vol 13 (1) ◽  
pp. 37-43
Author(s):  
Balu S. Khandare
Keyword(s):  
Regression Analysis ◽  
Linear Regression ◽  
High Performance ◽  
Linear Regression Analysis ◽  
Solvent System ◽  
Average Recovery ◽  
Bulk Drug ◽  
Development And Validation ◽  
Hptlc Method

This paper describes a simple, precise, rapid and accurate high- performance thin layer chromatographic (HPTLC) method for determination of Olmutinib in bulk drug. Chroma to graphic separation was per formed on aluminium plates precoated with silicagel60F254 as the stationary phase using solvent system consisted of chloro form: methanol: (9:1v/v). After the application of bands using CAMAG Automatic TLC Sampler 4, the plate was developed in the solvent system up to 70 mm in CAMAGT win Trough Chamber. This solvent system was found to give compact spot for Olmutinib with Rfvalue of 0.32±0.02. The spots were scanned at 267.68nm. The calibration curves were linear with co-relation coefficient of 0.995 for Olmutinib. Linear regression analysis showed good linearity in the concentration range of 100- 1100 ng per spot. The method was validated in terms of Precision, specificity, and Linearity. The average recovery of the standards in the samples was found to be 99.65% at the same time we have checked the C.V. values of Reproducibility, intra-day and inter-day tabulated further. The proposed method can be successfully applied to determine the drug content of bulk drug.

scholarly journals HPTLC Method Development and Validation for Determination of Rutin in Flavanoidal Fraction ofHibiscus micranthusLinn.

2011 ◽  
Vol 8 (3) ◽  
pp. 1444-1450 ◽  
Author(s):  
K. Ashok Kumar ◽  
S. Ramachandra Shetty ◽  
Laxmi Narasu
Keyword(s):  
High Performance ◽  
Method Development ◽  
Methanolic Extract ◽  
Method Development And Validation ◽  
Stem Extract ◽  
Acetic Acid Water ◽  
Flavonoid Fraction ◽  
Development And Validation ◽  
Hptlc Method

A simple, precise and accurate high-performance thin-layer chromatographic method has been established for the determination of rutin in the stem extract ofHibiscus micranthusLinn. A flavonoid fraction of methanolic extract of the stem powder was used for the experimental work. Separation was performed on silica gel 60 F254HPTLC plates withn-butanol: acetic acid: water: ammonia 3:1:1:1(v/v), as mobile phase. The determination was carried out using the densitometric absorbance mode at 286 nm. Rutin response was linear over the range 50–400 ng/μL. The concentration of rutin in the flavanoidal fraction was found to be 9.93%. The HPTLC method was evaluated in terms of sensitivity, accuracy, precision and reproducibility.

scholarly journals DEVELOPMENT AND VALIDATION OF STABILITY INDICATING HPTLC METHOD FOR DETERMINATION OF APIXABAN AS BULK DRUG

2019 ◽  
pp. 37-42
Author(s):  
Mrinalini C. Damle ◽  
Swapnil S Waghmare ◽  
PURUSHOTAM SINHA
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Limit Of Detection ◽  
Chromatography Method ◽  
Stability Indicating ◽  
Chromatographic Condition ◽  
Bulk Drug ◽  
Development And Validation ◽  
Hptlc Method

Objective: To develop and validate simple, sensitive stability indicating HPTLC (High performance thin layer chromatography) method for apixaban. Methods: The chromatographic separation was performed on aluminium plates precoated with silica gel 60 F254 using toluene: ethyl acetate: methanol (3:6:1 v/v/v) as mobile phase followed by densitometric scanning at 279 nm. Results: The chromatographic condition shows sharp peak of apixaban at Rf value of 0.38±0.03. Stress testing was carried out according to international conference on harmonization (ICH)Q1A (R2) guidelines and the method was validated as per ICH Q2(R1) guidelines. The calibration curve was found to be linear in the concentration range of 100-500 ng/band for apixaban. The limit of detection and quantification was found to be 11.66ng/bandand35.33ng/band, respectively. Conclusion: A new simple, sensitive, stability indicating high performance thin layer chromatographic (HPTLC) method has been developed and validated for the determination of apixaban.

scholarly journals IDENTIFICATION, QUANTIFICATION AND VALIDATION OF STIGMASTEROL FROM ALPINIA CALCARATA USING HIGH-PERFORMANCE THIN LAYER CHROMATOGRAPHY METHOD

2017 ◽  
Vol 9 (9) ◽  
pp. 126
Author(s):  
Pratheema Philomindoss
Keyword(s):  
Linear Regression ◽  
Thin Layer ◽  
Linear Relationship ◽  
High Performance ◽  
Retention Factor ◽  
Chromatography Method ◽  
Ich Guidelines ◽  
Layer Chromatography ◽  
Hptlc Method

Objective: The present study is designed to develop a new simple, precise, rapid and selective high‐performance thin‐layer chromatographic (HPTLC) method for the determination of stigmasterol in methanolic rhizomes extract of Alpinia calcarata.Methods: As per International Conference on Harmonization (ICH) guidelines we have applied different concentrations of stigmasterol as standard on HPTLC plates for the quantification of stigmasterol from the Alpinia calcarata rhizomes. The concentration of standard stigmasterol is 1 mg/ml.Results: The retention factor of stigmasterol was 0.58. Linearity was obtained in the range of 50 ng‐250 ng for stigmasterol. The developed and validated HPTLC method was employed for stigmasterol in methanolic rhizomes extract of Alpinia calcarata for standardization of the content of the marker. The linear regression data for the calibration plots showed a good linear relationship with r=0.99977 for stigmasterol, respectively Satisfactory recoveries of 99.77 % were obtained for stigmasterol.Conclusion: The results obtained in validation assays indicate the accuracy and reliability of the developed HPTLC method for the quantification of stigmasterol in methanolic rhizomes extract of Alpinia calcarata

scholarly journals DEVELOPMENT AND VALIDATION OF STABILITY INDICATING HPTLC METHOD FOR ESTIMATION OF SWERTIAMARIN IN BULK AND DOSAGE FORM

2017 ◽  
Vol 9 (6) ◽  
pp. 80
Author(s):  
H. Padh ◽  
S. Parmar ◽  
B. Patel
Keyword(s):  
High Performance ◽  
Forced Degradation ◽  
Stability Indicating ◽  
Bulk Drug ◽  
Development And Validation ◽  
Layer Chromatography ◽  
Herbal Formulations ◽  
Hptlc Method ◽  
Ich Guideline

Objective: In the present study a novel stability-indicating high-performance thin-layer chromatography (HPTLC) method for quantitative determination of Swertiamarin (SW) in bulk drug and formulation has been developed and validated as per ICH guideline Q2 (R1) for global acceptance of standardized herbal formulations.Methods: HPTLC method is developed and validated using solvent ethyl acetate: ethanol: chloroform (3:2.5:4.5 v/v/v) (Rf of SW 0.65±0.04) in the absorbance mode at 243 nm. Various forced degradation conditions were used to check degradation of drug.Results: The method showed a good linear relationship (r2 = 0.9990) in the concentration range 200-700 ng per spot. It was found to be linear, accurate, precise and specific.Conclusion: It can be applied for quality control as well as for stability testing of different dosage forms containing swertiamarin. The developed method is validated as per ICH guideline Q2(R1) for global acceptance of standardized herbal formulations.

scholarly journals Development and Validation of HPTLC Method for Studying Stress Degradation of Aspirin in Fulvic Acid Inclusion Complex

2020 ◽  
pp. 96-102
Author(s):  
Md. Khalid Anwer ◽  
Mohammed Muqtader Ahmed ◽  
Mohammad Javed Ansari ◽  
Mohammed F. Aldawsari ◽  
Mohd. Aamir Mirza
Keyword(s):  
Fulvic Acid ◽  
Inclusion Complexes ◽  
High Performance ◽  
Retention Factor ◽  
Molar Ratio ◽  
Forced Degradation ◽  
Ich Guidelines ◽  
Stress Degradation ◽  
Development And Validation ◽  
Hptlc Method

A new, precise high performance thin layer chromatographic (HPTLC) method for the analysis aspirin (ASP) in inclusion complexes with HPβCD and fulvic acid (FA) was developed and validated as per ICH guidelines. A precoated silica gel aluminium plate 60F-254 and a mixture of solvents, toluene: ethylacetate: formic acid (5:4:1 v/v) were used as stationary and mobile phase, respectively. This developed method was found to give an excellent defined sharp peak at a retention factor (RF) value of 0.52 ± 0.001. The LOQ and LOD values were found 35.29 and 123.54 ng / spot, respectively. The spray dried inclusion complexes of ASP/HPβCD and ASP/FA in the molar ratio 1:1, were subjected for forced degradation under stress conditions, and a significant reduction of ASP degradation were noted in complexed ASP as compared to ASP alone.

scholarly journals A Validated High Performance Thin Layer Chromatographic Method for Simultaneous Estimation of Berberine Chloride and Guggulsterone Z in Herbal Formulation

2019 ◽  
Vol 7 (1) ◽  
pp. 7-13
Author(s):  
Vijaykumar K. Parmar ◽  
Deepika Mohanta ◽  
Harsh Shah
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Simultaneous Estimation ◽  
Berberine Chloride ◽  
Herbal Formulation ◽  
Ich Guidelines ◽  
System Suitability ◽  
Layer Chromatography ◽  
Hptlc Method

A simple, precise, and robust high-performance thin layer chromatography (HPTLC) method was developed and validated for the determination of berberine chloride and guggulsterone Z in herbal formulation. Chromatographic separation was achieved on aluminium plates precoated with silica gel G60F254 as the stationary phase and toluene-acetonitrile-formic acid (5:3:0.5 v/v/v) as the mobile phase. Densitometric evaluation was carried out at 264 nm. The present method was validated according to ICH guidelines. The Rf value of berberine chloride and guggulsterone Z was found to be 0.40 ± 0.02 and 0.68 ± 0.02, respectively. The response in terms of peak area was found to be linear over the concentration range of 100-500 ng/spot for berberine chloride and 200-1000 ng/spot for guggulsterone Z with regression coefficient value greater than 0.995 for both the phytoconstituents. The method was validated by determining its accuracy, precision, robustness, specificity and system suitability. The method was found to be accurate, precise and robust to carry out the simultaneous estimation of berberine chloride and guggulsterone Z. The developed method was successfully applied for the simultaneous estimation of berberine chloride and guggulsterone Z in herbal formulation.

scholarly journals Highly Sensitive and Ecologically Sustainable Reversed-Phase HPTLC Method for the Determination of Hydroquinone in Commercial Whitening Creams

Processes ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1631
Author(s):  
Mohammed H. Alqarni ◽  
Prawez Alam ◽  
Faiyaz Shakeel ◽  
Ahmed I. Foudah ◽  
Sultan Alshehri
Keyword(s):  
Analytical Method ◽  
High Performance ◽  
Solvent System ◽  
Reversed Phase ◽  
Highly Sensitive ◽  
Validation Parameters ◽  
Ecologically Sustainable ◽  
Layer Chromatography ◽  
Hptlc Method

Hydroquinone (HDQ) is a natural depigmenting agent, which is commonly used in skin-toning preparations. The safety and greenness of analytical methods of HDQ quantification were not considered in previous literature. Therefore, a highly sensitive and ecologically greener reversed-phase high-performance thin-layer chromatography (RP-HPTLC)-based assay was established for HDQ estimation in four different commercial whitening creams (CWCs). The binary ethanol–water (60:40, v·v−1) mixture was utilized as the green solvent system. The estimation of HDQ was carried out at 291 nm. The present RP-HPTLC-based assay was linear in the 20–2400 ng band−1 range. The present analytical method was highly sensitive based on the detection and quantification data. The other validation parameters, such as accuracy, precision, and robustness, were also suitable for the determination of HDQ. Maximum HDQ quantities were obtained in CWC A (1.23% w·w−1) followed by CWC C (0.81% w·w−1), CWC D (0.43% w·w−1), and CWC B (0.37% w·w−1). The analytical GREEnness (AGREE) score for the present analytical method was estimated as 0.91, indicating the excellent greener characteristics of the present RP-HPTLC assay. These results suggest that the present analytical method is highly sensitive and ecologically sustainable for the quantitation of HDQ in its commercial formulations.

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