scholarly journals The Effect of Covalently Immobilized FGF-2 on Biphasic Calcium Phosphate Bone Substitute on Enhanced Biological Compatibility and Activity

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Kyung-Suk Moon ◽  
Eun-Joo Choi ◽  
Seunghan Oh ◽  
Sungtae Kim
Keyword(s):  
Calcium Phosphate ◽  
Osteogenic Differentiation ◽  
Biphasic Calcium Phosphate ◽  
Human Mesenchymal Stem Cells ◽  
Optimal Dose ◽  
Osteogenic Potential ◽  
Pcr Analysis ◽  
Control Group ◽  
Biological Compatibility ◽  
Cross Linker

The purpose of this research was to covalently graft fibroblast growth factor 2 (FGF-2) onto biphasic calcium phosphate (BCP) via a bifunctional cross-linker technique and to estimate the optimal dose of FGF-2 resulting in the best osteogenic differentiation of human mesenchymal stem cells (hMSCs). SEM observation revealed that the surface of the 100 ng FGF-2 coated BCP was completely covered with the nanoparticles expected to be from the silane coupling agent. XRD, FT-IR, and XPS analysis showed that silane treatment, bifunctional cross-linker coating, and FGF-2 covalent grafts were conducted successfully without deforming the crystalline structure of BCP. An MTT assay demonstrated that FGF-2 coated BCP had good biocompatibility, regardless of the concentration of FGF-2, after 24 or 48 h of incubation. An alkaline phosphatase (ALP) activity assay (14 days of incubation) and the ALP gene expression level of real-time PCR analysis (7 days of incubation) revealed that 50, 100, and 200 ng FGF-2 coated BCP induced the highest activities among all experimental groups and control group (P<0.05). Thus, low concentrations of FGF-2 facilitated excellent osteogenesis and were effective at enhancing osteogenic potential. Also, the bifunctional cross-linker technique is expected to be a more feasible way to induce osteogenic differentiation while minimizing the risk of FGF-2 overdose.

scholarly journals Osteogenic potential of poly(ethylene glycol)-amorphous calcium phosphate composites on human mesenchymal stem cells

2020 ◽  
Vol 11 ◽  
pp. 204173142092684 ◽  
Author(s):  
Aman S Chahal ◽  
Manuel Schweikle ◽  
Aina-Mari Lian ◽  
Janne E Reseland ◽  
Håvard J Haugen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Calcium Phosphate ◽  
Ethylene Glycol ◽  
Osteogenic Differentiation ◽  
Amorphous Calcium Phosphate ◽  
Human Mesenchymal Stem Cells ◽  
Osteogenic Potential ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

Synthetic hydrogel-amorphous calcium phosphate composites are promising candidates to substitute biologically sourced scaffolds for bone repair. While the hydrogel matrix serves as a template for stem cell colonisation, amorphous calcium phosphate s provide mechanical integrity with the potential to stimulate osteogenic differentiation. Here, we utilise composites of poly(ethylene glycol)-based hydrogels and differently stabilised amorphous calcium phosphate to investigate potential effects on attachment and osteogenic differentiation of human mesenchymal stem cells. We found that functionalisation with integrin binding motifs in the form of RGD tripeptide was necessary to allow adhesion of large numbers of cells in spread morphology. Slow dissolution of amorphous calcium phosphate mineral in the scaffolds over at least 21 days was observed, resulting in the release of calcium and zinc ions into the cell culture medium. While we qualitatively observed an increasingly mineralised extracellular matrix along with calcium deposition in the presence of amorphous calcium phosphate-loaded scaffolds, we did not observe significant changes in the expression of selected osteogenic markers.

Preparation of dexamethasone-loaded calcium phosphate nanoparticles for the osteogenic differentiation of human mesenchymal stem cells

2017 ◽  
Vol 5 (33) ◽  
pp. 6801-6810 ◽  
Author(s):  
Ying Chen ◽  
Jingchao Li ◽  
Naoki Kawazoe ◽  
Guoping Chen
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Calcium Phosphate ◽  
Osteogenic Differentiation ◽  
Biphasic Calcium Phosphate ◽  
Human Mesenchymal Stem Cells ◽  
Calcium Phosphate Nanoparticles

Dexamethasone (DEX)-loaded biphasic calcium phosphate nanoparticles (BCP-NPs) are prepared by incorporation of DEX during or after the formation of BCP-NPs. The DEX-loaded BCP-NPs release DEX in a sustained manner and enhance the osteogenic differentiation of hMSCs.

scholarly journals Comparative evaluation of osteogenic differentiation potential of stem cells derived from dental pulp and exfoliated deciduous teeth cultured over granular hydroxyapatite based scaffold

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Manal Nabil Hagar ◽  
Farinawati Yazid ◽  
Nur Atmaliya Luchman ◽  
Shahrul Hisham Zainal Ariffin ◽  
Rohaya Megat Abdul Wahab
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Dental Pulp ◽  
3D Culture ◽  
Permanent Teeth ◽  
Deciduous Teeth ◽  
Osteogenic Potential ◽  
Control Group ◽  
Rt Pcr ◽  
Differentiation Potential

Abstract Background Mesenchymal stem cells isolated from the dental pulp of primary and permanent teeth can be differentiated into different cell types including osteoblasts. This study was conducted to compare the morphology and osteogenic potential of stem cells from exfoliated deciduous teeth (SHED) and dental pulp stem cells (DPSC) in granular hydroxyapatite scaffold (gHA). Preosteoblast cells (MC3T3-E1) were used as a control group. Methodology The expression of stemness markers for DPSC and SHED was evaluated using reverse transcriptase-polymerase chain reaction (RT-PCR). Alkaline phosphatase assay was used to compare the osteoblastic differentiation of these cells (2D culture). Then, cells were seeded on the scaffold and incubated for 21 days. Morphology assessment using field emission scanning electron microscopy (FESEM) was done while osteogenic differentiation was detected using ALP assay (3D culture). Results The morphology of cells was mononucleated, fibroblast-like shaped cells with extended cytoplasmic projection. In RT-PCR study, DPSC and SHED expressed GAPDH, CD73, CD105, and CD146 while negatively expressed CD11b, CD34 and CD45. FESEM results showed that by day 21, dental stem cells have a round like morphology which is the morphology of osteoblast as compared to day 7. The osteogenic potential using ALP assay was significantly increased (p < 0.01) in SHED as compared to DPSC and MC3T3-E1 in 2D and 3D cultures. Conclusion gHA scaffold is an optimal scaffold as it induced osteogenesis in vitro. Besides, SHED had the highest osteogenic potential making them a preferred candidate for tissue engineering in comparison with DPSC.

Two-layer membranes of calcium phosphate/collagen/PLGA nanofibres: in vitro biomineralisation and osteogenic differentiation of human mesenchymal stem cells

Nanoscale ◽  
2011 ◽  
Vol 3 (2) ◽  
pp. 401-409 ◽  
Author(s):  
Nora Hild ◽  
Oliver D. Schneider ◽  
Dirk Mohn ◽  
Norman A. Luechinger ◽  
Fabian M. Koehler ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Calcium Phosphate ◽  
Osteogenic Differentiation ◽  
Human Mesenchymal Stem Cells

Impact of Biphasic Calcium Phosphate Bioceramics on Osteoporotic Hip Bone Mineralization In Vivo Six Months after Implantation

2016 ◽  
Vol 721 ◽  
pp. 229-233 ◽  
Author(s):  
Sandris Petronis ◽  
Janis Locs ◽  
Vita Zalite ◽  
Mara Pilmane ◽  
Andrejs Skagers ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Tricalcium Phosphate ◽  
Biphasic Calcium Phosphate ◽  
Bone Mineralization ◽  
Bone Substitutes ◽  
Tissue Response ◽  
Control Group ◽  
Osteoporotic Bone ◽  
Local Recovery

Calcium bone substitutes are successfully used for local recovery of osteoporotic bone and filling of bone defects. Previous studies revieled that biphasic calcium phosphate (BCP) show better bioactivity in compare to pure β-tricalcium phosphate or hydroxyapatite. Also increased porosity of material promotes better bone tissue response. Aim of this experiment was to evaluate immunohistologically response of osteoporotic bone of experimental animal to implantation of granules with hydroxyapatite/β-tricalcium phosphate (HAp/β-TCP) ratio of 90/10. Calcium phosphate (CaP) was synthesized by aqueous precipitation technique from calcium hydroxide and phosphoric acid. Bioceramic granules in size range from 1.0 to 1.4 mm were prepared with nanopore sizes around 200 nm. We used nine female rabbits with induced osteoporosis in this experiment. Six animals in study group underwent implantation of BCP in hip bone defect and three animals in control group left without BCP implantation. After 6 months animals were euthanized, bone samples collected and proceeded for detection of bone activity and repair markers: osteocalcin (OC), osteopontin (OP) and osteoprotegerin (OPG). Controls showed the presence of experimental bone osteoporosis. In experimental group bone showed partially resorbed bioceramic granules and in some samples new bone formation near the granuli was observed. Increase of OC and OPG up to twice as to compare to control group were detected as well. Implantation of BCP granules in osteoporotic rabbit bone increases expression of OC and OPG indicating the activation of osteoblastogenesis and bone mineralization in vivo.

scholarly journals Chitosan/ biphasic calcium phosphate nanofibrous scaffolds for bone tissue engineering

2020 ◽  
pp. 11-17
Author(s):  
Truong Le Bich Tram Truong
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Osteogenic Differentiation ◽  
Late Stage ◽  
Biphasic Calcium Phosphate ◽  
Cell Attachment ◽  
The Novel ◽  
Osteogenic Cells ◽  
Nanofibrous Scaffolds

In this article, chitosan/biphasic calcium phosphate (CS/BCP)nanofibers were prepared by electrospinning. From the culture of osteogenic cells, the biocompatibility of CS/BCP nanofibrous substrates was identified and increased by the photocrosslinking. The enhancement in cell attachment and proliferation was caused by the improvement in nanofibers’ mechanical properties. The biocompatibility to osteoblasts was also promoted with the content of BCP. The osteogenic differentiation in early, middle and late stage was encouraged by the addition of BCP on nanofibrous substrates. The CS/BCP nanofibers were highly specific to osteogenic cells, revealed by difficulties in the growth of non-osteogenic cells on this composite nanofibrous scaffold. The novel nanofibrous scaffolds showed great potential in the tissue engineering of bones.

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