Electrochemical Analysis of Ultrathin Polythiolsiloxane Films for Surface Biomodification

The ability of different crosslinkers to crosslink nanometer thick films of the polymer poly(mercaptopropyl)methylsiloxane (PMPMS), thus stabilizing these films on solid supports, was investigated. The four crosslinkers included 1,11-bismaleimidotriethyleneglycol (BM(PEG)3), tris-(2-maleimidoethyl)amine (TMEA), bismaleimidohexane (BMH), and 1,1′-(methylenedi-4,1-phenylene) bismaleimide (BMDPM). PMPMS films treated with the four crosslinkers were compared in the effectiveness of achieved crosslinking, continuity and stability of the films to rearrangement at elevated temperatures, and modification with single-stranded DNA. The results of electrochemical analyses show that more hydrophilic crosslinkers had difficulty reacting fully with PMPMS thiols, even in these nanometer thin layers. This observation highlights the critical importance of selecting crosslinkers that are chemically compatible. Optimal selection of crosslinker yielded films in which the polymer film was largely incapable of rearranging, even at elevated temperatures, yielding reproducible and stable layers. These results validate use of these supports for applications such as monitoring thermal denaturation of immobilized DNA duplexes.

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Optimal selection of sensor network and backed-off operating point based on economics

2013 European Control Conference (ECC) ◽

10.23919/ecc.2013.6669516 ◽

2013 ◽

Author(s):

Nabil Magbool Jan ◽

Sigurd Skogestad ◽

Sridharakumar Narasimhan

Keyword(s):

Sensor Network ◽

Operating Point ◽

Optimal Selection ◽

Selection Of

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Application of L6 Orthogonal Array for Optimal Selection of Some Process Parameters in GMAW Process

Indian Welding Journal ◽

10.22486/iwj.v45i4.141203 ◽

2012 ◽

Vol 45 (4) ◽

pp. 41 ◽

Cited By ~ 1

Author(s):

M. K. Saha ◽

Santanu Das ◽

A. Bandyopadhyay ◽

S. Bandyopadhyay

Keyword(s):

Process Parameters ◽

Orthogonal Array ◽

Optimal Selection ◽

Selection Of

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Application of L6 Orthogonal Array for Optimal Selection of Some Process Parameters in GMAW Process

Indian Welding Journal ◽

10.22486/iwj/2012/v45/i4/141203 ◽

2012 ◽

Vol 45 (4) ◽

pp. 41

Author(s):

M. K. Saha ◽

Santanu Das ◽

A. Bandyopadhyay ◽

S. Bandyopadhyay

Keyword(s):

Process Parameters ◽

Orthogonal Array ◽

Optimal Selection ◽

Selection Of

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Design and implementation of an ADC-based real-time simulator along with an optimal selection of the switch model parameters

Electrical Engineering ◽

10.1007/s00202-021-01237-1 ◽

2021 ◽

Author(s):

Morteza Rezaei Larijani ◽

Mohammad Reza Zolghadri

Keyword(s):

Real Time ◽

Model Parameters ◽

Optimal Selection ◽

Design And Implementation ◽

Selection Of

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Methodology for Determining the Location of River Ports on a Modernized Waterway Based on Non-Cost Criteria: A Case Study of the Odra River Waterway

Sustainability ◽

10.3390/su13063571 ◽

2021 ◽

Vol 13 (6) ◽

pp. 3571

Author(s):

Bogusz Wiśnicki ◽

Dorota Dybkowska-Stefek ◽

Justyna Relisko-Rybak ◽

Łukasz Kolanda

Keyword(s):

Large Scale ◽

Multicriteria Decision Making ◽

Research Process ◽

Optimal Selection ◽

Investment Projects ◽

Odra River ◽

Research Problems ◽

Selection Of ◽

Construction Works

The paper responds to research problems related to the implementation of large-scale investment projects in waterways in Europe. As part of design and construction works, it is necessary to indicate river ports that play a major role within the European transport network as intermodal nodes. This entails a number of challenges, the cardinal one being the optimal selection of port locations, taking into account the new transport, economic, and geopolitical situation that will be brought about by modernized waterways. The aim of the paper was to present an original methodology for determining port locations for modernized waterways based on non-cost criteria, as an extended multicriteria decision-making method (MCDM) and employing GIS (Geographic Information System)-based tools for spatial analysis. The methodology was designed to be applicable to the varying conditions of a river’s hydroengineering structures (free-flowing river, canalized river, and canals) and adjustable to the requirements posed by intermodal supply chains. The method was applied to study the Odra River Waterway, which allowed the formulation of recommendations regarding the application of the method in the case of different river sections at every stage of the research process.

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Improved strategies to efficiently isolate thermophilic, thermotolerant, and heat-resistant fungi from compost and soil

Mycological Progress ◽

10.1007/s11557-021-01674-z ◽

2021 ◽

Vol 20 (3) ◽

pp. 325-339

Author(s):

Frederick Witfeld ◽

Dominik Begerow ◽

Marco Alexandre Guerreiro

Keyword(s):

Elevated Temperatures ◽

Fungal Species ◽

Dna Barcodes ◽

Heat Shock Treatment ◽

Isolation And Identification ◽

Heat Resistant ◽

Heat Stable ◽

Promising Source ◽

Culture Dependent ◽

Selection Of

AbstractThermophilic, thermotolerant and heat-resistant fungi developed different physiological traits, enabling them to sustain or even flourish under elevated temperatures, which are life-hostile for most other eukaryotes. With the growing demand of heat-stable molecules in biotechnology and industry, the awareness of heat-adapted fungi as a promising source of respective enzymes and biomolecules is still increasing. The aim of this study was to test two different strategies for the efficient isolation and identification of distinctly heat-adapted fungi from easily accessible substrates and locations. Eight compost piles and ten soil sites were sampled in combination with different culture-dependent approaches to describe suitable strategies for the isolation and selection of thermophilous fungi. Additionally, an approach with a heat-shock treatment, but without elevated temperature incubation led to the isolation of heat-resistant mesophilic species. The cultures were identified based on morphology, DNA barcodes, and microsatellite fingerprinting. In total, 191 obtained isolates were assigned to 31 fungal species, from which half are truly thermophilic or thermotolerant, while the other half are heat-resistant fungi. A numerous amount of heat-adapted fungi was isolated from both compost and soil samples, indicating the suitability of the used approaches and that the richness and availability of those organisms in such environments are substantially high.

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Optimal Selection of Conductors in Three-Phase Distribution Networks Using a Discrete Version of the Vortex Search Algorithm

Computation ◽

10.3390/computation9070080 ◽

2021 ◽

Vol 9 (7) ◽

pp. 80

Author(s):

John Fernando Martínez-Gil ◽

Nicolas Alejandro Moyano-García ◽

Oscar Danilo Montoya ◽

Jorge Alexander Alarcon-Villamil

Keyword(s):

Objective Function ◽

Power Flow ◽

Phase Distribution ◽

Search Algorithm ◽

Distribution Networks ◽

Discrete Version ◽

Optimal Selection ◽

Flow Method ◽

Three Phase ◽

Selection Of

In this study, a new methodology is proposed to perform optimal selection of conductors in three-phase distribution networks through a discrete version of the metaheuristic method of vortex search. To represent the problem, a single-objective mathematical model with a mixed-integer nonlinear programming (MINLP) structure is used. As an objective function, minimization of the investment costs in conductors together with the technical losses of the network for a study period of one year is considered. Additionally, the model will be implemented in balanced and unbalanced test systems and with variations in the connection of their loads, i.e., Δ− and Y−connections. To evaluate the costs of the energy losses, a classical backward/forward three-phase power-flow method is implemented. Two test systems used in the specialized literature were employed, which comprise 8 and 27 nodes with radial structures in medium voltage levels. All computational implementations were developed in the MATLAB programming environment, and all results were evaluated in DigSILENT software to verify the effectiveness and the proposed three-phase unbalanced power-flow method. Comparative analyses with classical and Chu & Beasley genetic algorithms, tabu search algorithm, and exact MINLP approaches demonstrate the efficiency of the proposed optimization approach regarding the final value of the objective function.

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