Mass Spectrometry-Based Comprehensive Analysis of Pancreatic Cyst Fluids

Pancreatic cyst fluids (PCFs) enriched in tumour-derived proteins are considered a potential source of new biomarkers. This study aimed to determine compositional and quantitative differences between the degradome and proteome of PCFs aspirated from different types of pancreatic cyst lesions (PCLs). 91 patients who underwent endoscopic ultrasound-fine needle aspiration under routine clinical diagnosis of PCLs were enrolled. Four cysts were malignant (CAs), and 87 were nonmalignant and consisted of 18 intraductal papillary mucinous neoplasms (IPMNs), 14 mucinous cystic neoplasms (MCNs), nine serous cystic neoplasms (SCNs), 29 pseudocysts (PCs), and 17 unclassified. Profiles of the <5 kDa fraction, the degradome, and the trypsin-digested proteome were analysed using an LTQ-Orbitrap Elite mass spectrometer coupled with a nanoACQUITY LC system. Qualitative analyses identified 796 and 366 proteins in degradome and proteome, respectively, and 689 (77%) and 285 (78%) of them were present in the Plasma Proteome Database. Gene Ontology analysis showed a significant overrepresentation of peptidases and peptidases inhibitors in both datasets. In the degradome fraction, quantitative values were obtained for 6996 peptides originating from 657 proteins. Of these, 2287 peptides were unique to a single type, and 515 peptides, derived from 126 proteins, were shared across cyst types. 32 peptides originating from 12 proteins had differential (adjustedp-value ≤0.05, FC ≥1.5) abundance in at least one of the five cysts types. In proteome, relative expression was measured for 330 proteins. Of them, 33 proteins had significantly (adjustedp-value ≤0.05, FC ≥1.5) altered abundance in at least one of the studied groups and 19 proteins appeared to be unique to a given cyst type. PCFs are dominated by blood proteins and proteolytic enzymes. Although differences in PCF peptide composition and abundance could aid classification of PCLs, the unpredictable inherent PCF proteolytic activity may limit the practical applications of PCF protein profiling.

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Diagnostic yield of endoscopic ultrasonography (EUS), EUS-guided fine-needle aspiration (EUS-FNA), cyst fluid tumour markers & cytology in pancreatic cystic neoplasms

Endoscopy ◽

10.1055/s-2006-947645 ◽

2006 ◽

Vol 39 (S 1) ◽

Author(s):

R Repak ◽

S Rejchrt ◽

J Bartova ◽

E Malirova ◽

V Tycova ◽

...

Keyword(s):

Endoscopic Ultrasonography ◽

Fine Needle Aspiration ◽

Diagnostic Yield ◽

Needle Aspiration ◽

Tumour Markers ◽

Cyst Fluid ◽

Cystic Neoplasms ◽

Fine Needle ◽

Pancreatic Cystic Neoplasms

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Thermal Liquid Biopsy (TLB) Focused on Benign and Premalignant Pancreatic Cyst Diagnosis

Journal of Personalized Medicine ◽

10.3390/jpm11010025 ◽

2020 ◽

Vol 11 (1) ◽

pp. 25

Author(s):

Sonia Hermoso-Durán ◽

Guillermo García-Rayado ◽

Laura Ceballos-Laita ◽

Carlos Sostres ◽

Sonia Vega ◽

...

Keyword(s):

Liquid Biopsy ◽

Pancreatic Cyst ◽

Point Of View ◽

Invasive Technique ◽

Background Current ◽

Interesting Point ◽

Pancreatic Lesion ◽

Non Invasive ◽

Patient Diagnosis ◽

New Biomarkers

Background: Current efforts in the identification of new biomarkers are directed towards an accurate differentiation between benign and premalignant cysts. Thermal Liquid Biopsy (TLB) has been previously applied to inflammatory and tumor diseases and could offer an interesting point of view in this type of pathology. Methods: In this work, twenty patients (12 males and 8 females, average ages 62) diagnosed with a pancreatic cyst benign (10) and premalignant (10) cyst lesions were recruited, and biological samples were obtained during the endoscopic ultrasonography procedure. Results: Proteomic content of cyst liquid samples was studied and several common proteins in the different groups were identified. TLB cyst liquid profiles reflected protein content. Also, TLB serum score was able to discriminate between healthy and cysts patients (71% sensitivity and 98% specificity) and between benign and premalignant cysts (75% sensitivity and 67% specificity). Conclusions: TLB analysis of plasmatic serum sample, a quick, simple and non-invasive technique that can be easily implemented, reports valuable information on the observed pancreatic lesion. These preliminary results set the basis for a larger study to refine TLB serum score and move closer to the clinical application of TLB providing useful information to the gastroenterologist during patient diagnosis.

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Bilayer graphene oxide membranes for a wearable hemodialyzer

10.1101/2020.05.26.116863 ◽

2020 ◽

Author(s):

Richard P. Rode ◽

Henry H. Chung ◽

Hayley N. Miller ◽

Thomas R. Gaborski ◽

Saeed Moghaddam

Keyword(s):

Graphene Oxide ◽

Cytochrome C ◽

Self Assembly ◽

Bilayer Membrane ◽

Blood Proteins ◽

High Mechanical Strength ◽

Practical Applications ◽

New Process ◽

Improved Performance ◽

Oxide Membranes

2D nanomaterials have long been considered for development of ultra-high throughput membranes, due to their atomically thin nature and high mechanical strength. However, current processes have yet to yield a viable membrane for practical applications due to the lack of scalability and substantially improved performance over existing membranes. Herein, a graphene oxide (GO) bilayer membrane with a permeability of 1562 mL/hr.mmHg.m2, two orders of magnitude higher than existing nanofiltration membranes, and a tight molecular weight cut-off (MWCO) is presented. To build such a membrane, we have developed a new process involving self-assembly and optimization of GO nanoplatelets physicochemical properties. The process produced a highly organized mosaic of nanoplatelets enabling ultra-high permeability and selectivity with only three layers of GO. Performance of the membrane has been evaluated in a simulated hemodialysis application, where it presents a great value proposition. The membrane has a precise molecular cut-off size of 5 nm, adjusted using a molecular interlinker, designed to prevent loss of critical blood proteins. Urea, cytochrome-c, and albumin are used as representative test molecules. Urea and cytochrome-c sieving coefficients of 0.5 and 0.4 were achieved under physiological pressure conditions, while retaining 99% of albumin. Hemolysis, complement activation, and coagulation studies exhibit a performance on par or superior to the existing hemodialyzer materials.

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Successful use of endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) on a pancreatic lesion in a peritoneal dialysis patient without interrupting treatment

Peritoneal Dialysis International ◽

10.1177/0896860819893579 ◽

2020 ◽

Vol 40 (2) ◽

pp. 230-232 ◽

Cited By ~ 2

Author(s):

Susie Q Lew ◽

Ali A Khan ◽

Brandon Rieders ◽

Satyanisth T Agrawal

Keyword(s):

Peritoneal Dialysis ◽

Endoscopic Ultrasound ◽

Fine Needle Aspiration ◽

Dialysis Patient ◽

Pancreatic Cyst ◽

Peritoneal Dialysis Patient ◽

Needle Aspiration ◽

Ultrasound Guided ◽

Pancreatic Lesion ◽

Fine Needle

Endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA), a well-established minimally invasive gastrointestinal procedure, has been used to diagnose and stage cancers of the pancreas. We describe the successful use of EUS-FNA in a peritoneal dialysis (PD) patient to evaluate a pancreatic cyst. The patient continued on PD immediately after the procedure without using hemodialysis. The patient did not experience any complication such as infection, bleeding, or peritoneal fluid leakage.

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Survival in Cystic Neoplasms of the Pancreas

Canadian Journal of Gastroenterology ◽

10.1155/2009/139780 ◽

2009 ◽

Vol 23 (8) ◽

pp. 537-542 ◽

Cited By ~ 7

Author(s):

Ali Cadili ◽

Amy Bazzerelli ◽

Sipi Garg ◽

Robert Bailey

Keyword(s):

Overall Survival ◽

Pancreatic Cyst ◽

Patient Survival ◽

Survival Rates ◽

Pancreatic Cysts ◽

Cystic Neoplasms ◽

Patient Age ◽

Patient Âgé ◽

History Of ◽

Pancreatic Cystic Neoplasms

BACKGROUND: The natural history of pancreatic cystic neoplasms remains poorly understood despite growing evidence on the subject. Pancreatic cysts display a wide spectrum of pathological phenotypes, each associated with a different prognostic implication. Many pancreatic cysts are of undetermined malignant potential at presentation and remain so until surgically resected. While the survival rates of patients with malignant cysts are known to be poor, survival rates in patients with undetermined pancreatic cysts are unknown.OBJECTIVE: To identify the factors associated with survival in a group of patients diagnosed with a pancreatic cyst(s).METHODS: The present study was a retrospective multicentre review of pancreatic cystic neoplasms. All patients with a diagnosis of a neoplastic pancreatic cyst from 1994 to 2003 were identified at five different institutions in Edmonton, Alberta. The data collected included patient age, sex, imaging modality, cyst location, cyst size, number of cysts, comorbid illnesses, history of upper abdominal surgery, previous cancer, previous or concurrent metastases, symptoms (pain, upper gastrointestinal bleeding, signs of biliary obstruction, nausea/vomiting), remarkable radiological features, elevated amylase or lipase, type of pancreatic surgery, final pathology (benign or malignant) and overall survival. Survival models were used to assess whether any covariates were predictors of the survival time. Patient data were plotted using the Kaplan-Meier method. The resulting plot was used to calculate survival in the cohort.RESULTS: In total, 64 patients were identified as having neoplastic pancreatic cysts from 1994 to 2003 at the five institutions. The median overall patient survival time was 86 months. The median age at diagnosis for the patient population was 73 years, with 40 patients being women. Univariate analysis revealed that the risk of death was associated with patient age, sex and history of major comorbid illness. Multivariate models identified increased patient age and male sex as the factors that correlated most strongly with decreased overall survival.CONCLUSION: Overall survival in patients with neoplastic pancreatic cysts is determined by patient factors (ie, age and sex) rather than factors descriptive of the cyst such as size and morphology. No conclusions could be made regarding the relationship between cyst pathology and patient survival.

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Mucinous cystic adenoma with inflammatory cell infiltration around the splenic artery mimicking pancreatic cancer: a case report

Clinical Journal of Gastroenterology ◽

10.1007/s12328-020-01228-y ◽

2020 ◽

Vol 13 (6) ◽

pp. 1360-1366

Author(s):

Hiroaki Okuse ◽

Reiko Yamada ◽

Kyosuke Tanaka ◽

Noriyuki Horiki ◽

Yoshiyuki Takei

Keyword(s):

Distal Pancreatectomy ◽

Splenic Artery ◽

Inflammatory Cell ◽

Inflammatory Cells ◽

Needle Aspiration ◽

Inflammatory Cell Infiltration ◽

Cell Infiltration ◽

Low Grade ◽

Cystic Neoplasms ◽

Cystic Adenoma

AbstractA 45-year-old woman presented with upper abdominal and back pain. A cystic lesion in the pancreas and inflammation around the splenic artery were detected by computed tomography. Although imaging studies were difficult to exclude malignancy, pathological and cytological findings of a fine-needle aspiration showed no signs of malignancy. The patient was, therefore, followed-up for 3 months, during which time the cyst increased in size and developed a cyst-in-cyst structure. She was diagnosed with mucinous cyst neoplasm and underwent distal pancreatectomy. Histologically, the patient was diagnosed as low-grade mucinous cystic adenoma. Soft tissue shadows around the splenic artery were considered to indicate fibrosis and infiltration of inflammatory cells. After distal pancreatectomy, the patient has been uneventful with symptom resolution. This case highlights the potentially atypical presentation of mucinous cystic neoplasms with inflammatory cell infiltration around the splenic artery.

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