Genotoxicity of Copper and Nickel Nanoparticles in Somatic Cells of Drosophila melanogaster

Copper and nickel nanoparticles (Cu-NPs and Ni-NPs, respectively) are used in a variety of industrial applications, such as semiconductors, catalysts, sensors, and antimicrobial agents. Although studies on its potential genotoxicity already exist, few of them report in vivo data. In the present study we have used the wing-spot assay in Drosophila melanogaster to determine the genotoxic activity of Cu-NPs and Ni-NPs, and these data have been compared with those obtained with their microparticle forms (MPs). Additionally, a complete physical characterization of NPs using transmission electronic microscopy (TEM), dynamic light scattering (DLS), and laser Doppler velocimetry (LDV) techniques was also performed. Results obtained with Cu-NPs and Cu-MPs indicate that both failed to induce an increase in the frequency of mutant spots formation in the wings of the adults, suggesting a lack of genotoxicity in somatic cells of D. melanogaster. However, when Ni-NPs and Ni-MPs were evaluated, a significant increase of small single spots and total mutant spots was observed only for Ni-NPs (P<0.05) at the highest dose assessed. Thus, the genotoxicity of Ni-NPs seem to be related to their nanoscale size, because no genotoxic effects have been reported with their microparticles and ions. This study is the first assessing the in vivo genotoxic potential of Cu-NPs and Ni-NPs in the Drosophila model.

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Synthesis, Quantification, and Characterization of Fatty Acid Amides from In Vitro and In Vivo Sources

Molecules ◽

10.3390/molecules26092543 ◽

2021 ◽

Vol 26 (9) ◽

pp. 2543

Author(s):

Ruidong Ni ◽

Suzeeta Bhandari ◽

Perry R. Mitchell ◽

Gabriela Suarez ◽

Neel B. Patel ◽

...

Keyword(s):

Drosophila Melanogaster ◽

Fatty Acid ◽

Apis Mellifera ◽

Chemical Synthesis ◽

Acid Amide ◽

Fatty Acid Amides ◽

Fatty Acid Amide

Fatty acid amides are a diverse family of underappreciated, biologically occurring lipids. Herein, the methods for the chemical synthesis and subsequent characterization of specific members of the fatty acid amide family are described. The synthetically prepared fatty acid amides and those obtained commercially are used as standards for the characterization and quantification of the fatty acid amides produced by biological systems, a fatty acid amidome. The fatty acid amidomes from mouse N18TG2 cells, sheep choroid plexus cells, Drosophila melanogaster, Bombyx mori, Apis mellifera, and Tribolium castaneum are presented.

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Isolation and characterization of the dopa decarboxylase gene of Drosophila melanogaster

Molecular and Cellular Biology ◽

10.1128/mcb.1.6.475-485.1981 ◽

1981 ◽

Vol 1 (6) ◽

pp. 475-485

Author(s):

J Hirsh ◽

N Davidson

Keyword(s):

Drosophila Melanogaster ◽

Ribonucleic Acid ◽

Deoxyribonucleic Acid ◽

Isolation Procedure ◽

Chromosome Band ◽

Dopa Decarboxylase ◽

Developmental Profile ◽

Isolation And Characterization

We have isolated chromosomal deoxyribonucleic acid clones containing the Drosophila dopa decarboxylase gene. We describe an isolation procedure which can be applied to other nonabundantly expressed Drosophila genes. The dopa decarboxylase gene lies within or very near polytene chromosome band 37C1-2. The gene is interrupted by at least one intron, and the primary mode of regulation is pretranslational. At least two additional sequences hybridized by in vivo ribonucleic acid-derived probes are found within a 35-kilobase region surrounding the gene. The developmental profile of ribonucleic acid transcribed from one of these regions differs from that of the dopa decarboxylase transcript.

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Sortase-mediated site-specific modification of interleukin-2 for the generation of a tumor targeting acetazolamide-cytokine conjugate

10.1101/2020.07.20.211870 ◽

2020 ◽

Author(s):

Baptiste Gouyou ◽

J Millul ◽

A Villa ◽

S Cazzamalli ◽

D Neri ◽

...

Keyword(s):

Carbonic Anhydrase ◽

Tumor Targeting ◽

Interleukin 2 ◽

Industrial Applications ◽

Carbonic Anhydrase Ix ◽

Site Specific ◽

Tumor Bearing ◽

Bioactive Proteins

1.AbstractSmall ligands specific to tumor-associated antigens can be used as alternatives to antibodies for the delivery of small payloads such as radionuclides, cytotoxic drugs and fluorophores. Their use as delivery moiety of bioactive proteins like cytokines remains largely unexplored. Here, we describe the preparation and in vivo characterization of the first small molecule-cytokine conjugate targeting carbonic anhydrase IX (CAIX), a marker of renal cell carcinoma and hypoxia. Site-specific conjugation between interleukin-2 and acetazolamide was obtained by Sortase A-mediated transpeptidation. Binding of the conjugate to the cognate CAIX antigen was confirmed by surface plasmon resonance. The in vivo targeting of structures expressing carbonic anhydrase IX was assessed by biodistribution experiments in tumor bearing mice. Optimization of manufacturability and tumor targeting performance of acetazolamide-cytokine products will be required in order to enable industrial applications.Graphical abstract

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Identification and characterization of DAlk: a novel Drosophila melanogaster RTK which drives ERK activation in vivo

Genes to Cells ◽

10.1046/j.1365-2443.2001.00440.x ◽

2001 ◽

Vol 6 (6) ◽

pp. 531-544 ◽

Cited By ~ 73

Author(s):

Christina E. Lorén ◽

Audra Scully ◽

Caroline Grabbe ◽

Philip T. Edeen ◽

John Thomas ◽

...

Keyword(s):

Drosophila Melanogaster ◽

Erk Activation ◽

Identification And Characterization

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Genotoxic and safety assessment of 2 parabens in somatic cells of in vivo Drosophila melanogaster

TURKISH JOURNAL OF BIOLOGY ◽

10.3906/biy-1303-60 ◽

2013 ◽

Vol 37 ◽

pp. 683-688 ◽

Cited By ~ 4

Author(s):

Arif AYAR ◽

Handan UYSAL

Keyword(s):

Drosophila Melanogaster ◽

Safety Assessment ◽

Somatic Cells

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DNA-damaging potency and genotoxicity of aflation M1 in somatic cells in vivo of Drosophila melanogaster

Mutagenesis ◽

10.1093/mutage/10.3.161 ◽

1995 ◽

Vol 10 (3) ◽

pp. 161-164 ◽

Cited By ~ 12

Author(s):

Toshikazu Shibahara ◽

H.Iyehara Ogawa ◽

Haruko Ryo ◽

Kazuo Fujikawa

Keyword(s):

Drosophila Melanogaster ◽

Somatic Cells

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The Histone H3 Acetylase dGcn5 Is a Key Player in Drosophila melanogaster Metamorphosis

Molecular and Cellular Biology ◽

10.1128/mcb.25.18.8228-8238.2005 ◽

2005 ◽

Vol 25 (18) ◽

pp. 8228-8238 ◽

Cited By ~ 60

Author(s):

Clément Carré ◽

Dimitri Szymczak ◽

Josette Pidoux ◽

Christophe Antoniewski

Keyword(s):

Drosophila Melanogaster ◽

Histone H3 ◽

Noticeable Effect ◽

Developmental Transitions ◽

Isolation And Characterization ◽

Lysine Residues ◽

Developmental Role ◽

Variant Protein

ABSTRACT Although it has been well established that histone acetyltransferases (HATs) are involved in the modulation of chromatin structure and gene transcription, there is only little information on their developmental role in higher organisms. Gcn5 was the first transcription factor with HAT activity identified in eukaryotes. Here we report the isolation and characterization of Drosophila melanogaster dGcn5 mutants. Null dGcn5 alleles block the onset of both oogenesis and metamorphosis, while hypomorphic dGcn5 alleles impair the formation of adult appendages and cuticle. Strikingly, the dramatic loss of acetylation of the K9 and K14 lysine residues of histone H3 in dGcn5 mutants has no noticeable effect on larval tissues. In contrast, strong cell proliferation defects in imaginal tissues are observed. In vivo complementation experiments revealed that dGcn5 integrates specific functions in addition to chromosome binding and acetylation. Surprisingly, a dGcn5 variant protein with a deletion of the bromodomain, which has been shown to recognize acetylated histones, appears to be fully functional. Our results establish dGcn5 as a major histone H3 acetylase in Drosophila which plays a key role in the control of specific morphogenetic cascades during developmental transitions.

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GENETIC LOCALIZATION AND BIOCHEMICAL CHARACTERIZATION OF A TRANS-ACTING REGULATORY EFFECT IN DROSOPHILA

Genetics ◽

10.1093/genetics/105.1.55 ◽

1983 ◽

Vol 105 (1) ◽

pp. 55-69

Author(s):

Joseph J King ◽

John F McDonald

Keyword(s):

Drosophila Melanogaster ◽

Biochemical Characterization ◽

Regulatory Gene ◽

Regulatory Effect ◽

Protein Levels ◽

The Third ◽

Genetic Localization ◽

Glycerophosphate Dehydrogenase

ABSTRACT A region-specific, trans-acting regulatory gene that alters in vivo protein levels of α-glycerophosphate dehydrogenase (α-GPDH) has been mapped to position 55.4 on the third chromosome of Drosophila melanogaster. The gene has been found to affect the in vivo stability of α-GPDH in adult thoracic tissue but has no effect on α-GPDH levels in the abdomen. Although no other thoracic proteins were found to be influenced by the locus, it appears to modify the level of one additional abdominal protein. The action of the gene over development and its possible mode of control are discussed.

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Isolation and Characterization of a Novel Lytic Bacteriophage against the K2 Capsule-Expressing Hypervirulent Klebsiella pneumoniae Strain 52145, and Identification of Its Functional Depolymerase

Microorganisms ◽

10.3390/microorganisms9030650 ◽

2021 ◽

Vol 9 (3) ◽

pp. 650

Author(s):

Botond Zsombor Pertics ◽

Alysia Cox ◽

Adrienn Nyúl ◽

Nóra Szamek ◽

Tamás Kovács ◽

...

Keyword(s):

Klebsiella Pneumoniae ◽

Antimicrobial Agents ◽

Lytic Bacteriophage ◽

Bacterial Cells ◽

Narrow Host Range ◽

Isolation And Characterization ◽

Different Temperatures ◽

Colonization Model

Klebsiella pneumoniae is among the leading bacteria that cause nosocomial infections. The capsule of this Gram-negative bacterium is a dominant virulence factor, with a prominent role in defense and biofilm formation. Bacteriophages, which are specific for one bacterial strain and its capsule type, can evoke the lysis of bacterial cells, aided by polysaccharide depolymerase enzymes. In this study, we isolated and characterized a bacteriophage against the nosocomial K. pneumoniae 52145 strain with K2 capsular serotype. The phage showed a narrow host range and stable lytic activity, even when exposed to different temperatures or detergents. Preventive effect of the phage in a nasal colonization model was investigated in vivo. Phlyogenetic analysis showed that the newly isolated Klebsiella phage B1 belongs to the Webervirus genus in Drexlerviridae family. We identified the location of the capsule depolymerase gene of the new phage, which was amplified, cloned, expressed, and purified. The efficacy of the recombinant B1dep depolymerase was tested by spotting on K. pneumoniae strains and it was confirmed that the extract lowers the thickness of the bacterium lawn as it degrades the protective capsule on bacterial cells. As K. pneumoniae strains possessing the K2 serotype have epidemiological importance, the B1 phage and its depolymerase are promising candidates for use as possible antimicrobial agents.

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