Therapeutic Effects of Tangshen Formula on Diabetic Nephropathy in db/db Mice Using Cytokine Antibody Array

Objective. Cytokines are essential promoters in the pathogenesis of diabetic nephropathy (DN) in type 2 diabetes. The following study investigates the adjustment mechanism of Tangshen formula (TSF) on cytokine expressions in db/db mice (DN animal model). Materials and Methods. Db/db mice were randomly divided into three groups. The treated groups were orally administered with TSF and losartan for 12 weeks. Biochemical and histological examinations were determined at 8 and 12 weeks posttreatment, while the cytokine antibody array analysis was applied to analyze the expression of 144 cytokines in kidney tissues at the end of the 12th week posttreatment. Results. TSF significantly reduced urinary albumin excretion and the levels of blood glucose, cholesterol, triglyceride, creatinine, and urea nitrogen. Furthermore, a significant decrease in glomerulus and mesangial area, as well as the downregulation of 24 cytokines and upregulated expressions of 5 cytokines, was found in the TSF-treated mice. Conclusions. The present study reveals that TSF could ameliorate the metabolic anomalies and renal injury in db/db mice. One of the important mechanisms for treatment of DN using the treatment of TSF is the control of the JAK/STAT signaling pathway via regulation of IL-2, IL-6, IL-13, Il-15, and IFN-γ expression.

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Abstract: P1233 RELATION OF APOLIPOPROTEIN-B TO URINARY ALBUMIN EXCRETION IN EGYPTIAN PATIENTS WITH TYPE 2 DIABETES MELLITUS AND EARLY DIABETIC NEPHROPATHY

Atherosclerosis Supplements ◽

10.1016/s1567-5688(09)71250-4 ◽

2009 ◽

Vol 10 (2) ◽

pp. e1288

Author(s):

R Shafeh ◽

T Abdel-Aaty

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Diabetic Nephropathy ◽

Apolipoprotein B ◽

Urinary Albumin Excretion ◽

Urinary Albumin ◽

Egyptian Patients ◽

Early Diabetic Nephropathy

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Administration of RAS Inhibitor before the Onset of Diabetic Nephropathy Counteracts the Adverse Effect of Chronic Hyperglycemia and Reduces the Augmentation of Urinary Albumin Excretion: A Retrospective Clinical Study

Journal of Diabetes Research ◽

10.1155/2018/9435401 ◽

2018 ◽

Vol 2018 ◽

pp. 1-5

Author(s):

Hidenori Hirukawa ◽

Shinji Kamei ◽

Tomohiko Kimura ◽

Atsushi Obata ◽

Kenji Kohara ◽

...

Keyword(s):

Type 2 Diabetes ◽

Renal Function ◽

Diabetic Nephropathy ◽

Urinary Albumin Excretion ◽

Urinary Albumin ◽

Albumin Excretion ◽

Chronic Hyperglycemia ◽

Retrospective Clinical Study ◽

Japanese Subjects

It is very important to explore how we can reduce urinary albumin excretion which is an independent risk factor for ischemic heart disease. In this study, we retrospectively evaluated the effects of RAS inhibitor therapy on diabetic nephropathy in Japanese subjects whose urinary albumin levels were within normal range. We enrolled 100 subjects with type 2 diabetes who did not take any renin-angiotensin system (RAS) inhibitor. We defined the subjects taking RAS inhibitor for more than 3 years as RAS inhibitor group. RAS inhibitor exerted protective effect on the progression of urinary albumin excretion in subjects with type 2 diabetes without diabetic nephropathy. In addition, RAS inhibitor exerted more protective effects on renal function especially in subjects with poor glycemic control. In conclusion, RAS inhibitor could protect renal function against the deleterious effect of chronic hyperglycemia in Japanese subjects with type 2 diabetes even before the onset of diabetic nephropathy.

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Elevated specific peripheral cytokines found in major depressive disorder patients with childhood trauma exposure: A cytokine antibody array analysis

Comprehensive Psychiatry ◽

10.1016/j.comppsych.2013.03.026 ◽

2013 ◽

Vol 54 (7) ◽

pp. 953-961 ◽

Cited By ~ 56

Author(s):

Shaojia Lu ◽

Hongjun Peng ◽

Lifeng Wang ◽

Seewoobudul Vasish ◽

Yan Zhang ◽

...

Keyword(s):

Major Depressive Disorder ◽

Depressive Disorder ◽

Childhood Trauma ◽

Trauma Exposure ◽

Antibody Array ◽

Array Analysis ◽

Major Depressive ◽

Cytokine Antibody Array

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Association of serum pentraxin 3 concentrations with diabetic nephropathy

Journal of Investigative Medicine ◽

10.1136/jim-2016-000082 ◽

2016 ◽

Vol 64 (6) ◽

pp. 1124-1127 ◽

Cited By ~ 7

Author(s):

Rui Wang ◽

Jietao Zhang ◽

Wenchao Hu

Keyword(s):

Diabetic Nephropathy ◽

Healthy Subjects ◽

Renal Damage ◽

Urinary Albumin Excretion ◽

Urinary Albumin ◽

Pentraxin 3 ◽

Elisa Kit ◽

Simple Regression Analysis ◽

Simple Regression

Pentraxin 3 (PTX3), a member of a superfamily of conserved proteins, attenuates renal damage in diabetic mice. This study aims to determine whether serum PTX3 concentrations are correlated with the presence of diabetic nephropathy (DN). A total of 160 patients with type 2 diabetes mellitus (T2DM) and 54 healthy subjects were enrolled in this study. Patients with T2DM were divided into three groups in accordance with the levels of urinary albumin excretion (UAE). Serum PTX3 concentrations were determined using an ELISA kit. Serum PTX3 concentrations were significantly higher in patients with T2DM compared with the controls. Patients with T2DM with macroalbuminuria showed higher serum PTX3 concentrations compared with the other three groups. However, there were no significant differences of serum PTX3 concentrations between patients with T2DM with normoalbuminuria and microalbuminuria. Furthermore, a simple regression analysis has shown that serum PTX3 concentrations in patients with T2DM were negatively correlated with body mass index, and positively correlated with blood urea nitrogen, serum creatinine, and UAE. Serum PTX3 concentrations are correlated with DN.

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Effects of Different Doses of Irbesartan Combined With Spironolactone on Urinary Albumin Excretion Rate in Elderly Patients With Early Type 2 Diabetic Nephropathy

The American Journal of the Medical Sciences ◽

10.1016/j.amjms.2018.01.017 ◽

2018 ◽

Vol 355 (5) ◽

pp. 418-424 ◽

Cited By ~ 6

Author(s):

Yingying Chen ◽

Peng Liu ◽

Xia Chen ◽

Yanan Li ◽

Fengmei Zhang ◽

...

Keyword(s):

Diabetic Nephropathy ◽

Urinary Albumin Excretion ◽

Excretion Rate ◽

Albumin Excretion Rate ◽

Urinary Albumin ◽

Urinary Albumin Excretion Rate ◽

Early Type ◽

Type 2 Diabetic ◽

Different Doses

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Amelioration of renal alterations in obese type 2 diabetic mice by vasohibin-1, a negative feedback regulator of angiogenesis

AJP Renal Physiology ◽

10.1152/ajprenal.00503.2010 ◽

2011 ◽

Vol 300 (4) ◽

pp. F873-F886 ◽

Cited By ~ 39

Author(s):

Daisuke Saito ◽

Yohei Maeshima ◽

Tatsuyo Nasu ◽

Hiroko Yamasaki ◽

Katsuyuki Tanabe ◽

...

Keyword(s):

Diabetic Nephropathy ◽

Therapeutic Efficacy ◽

Transforming Growth Factor ◽

Angiogenesis Inhibitor ◽

Therapeutic Effects ◽

Mrna Levels ◽

Diabetic Mice ◽

Protein Levels ◽

Type 2 Diabetic

The involvement of VEGF-A as well as the therapeutic efficacy of angiogenesis inhibitors in diabetic nephropathy have been reported. We recently reported the therapeutic effects of vasohibin-1 (VASH-1), an endogenous angiogenesis inhibitor, in a type 1 diabetic nephropathy model (Nasu T, Maeshima Y, Kinomura M, Hirokoshi-Kawahara K, Tanabe K, Sugiyama H, Sonoda H, Sato Y, Makino H. Diabetes 58: 2365–2375, 2009). In this study, we investigated the therapeutic efficacy of VASH-1 on renal alterations in obese mice with type 2 diabetes. Diabetic db/db mice received intravenous injections of adenoviral vectors encoding human VASH-1 (AdhVASH-1) and were euthanized 8 wk later. AdhVASH-1 treatment resulted in significant suppression of glomerular hypertrophy, glomerular hyperfiltration, albuminuria, increase in the CD31+ glomerular endothelial area, F4/80+ monocyte/macrophage infiltration, the accumulation of type IV collagen, and mesangial matrix. An increase in the renal levels of VEGF-A, VEGFR-2, transforming growth factor (TGF)-β1, and monocyte chemoattractant protein-1 in diabetic animals was significantly suppressed by AdhVASH-1 (immunoblotting). AdhVASH-1 treatment significantly recovered the loss and altered the distribution patterns of nephrin and zonula occludens (ZO)-1 and suppressed the increase in the number of fibroblast-specific protein-1 (FSP-1+) and desmin+ podocytes in diabetic mice. In vitro, recombinant human VASH-1 (rhVASH-1) dose dependently suppressed the upregulation of VEGF induced by high ambient glucose (25 mM) in cultured mouse podocytes. In addition, rhVASH-1 significantly recovered the mRNA levels of nephrin and the protein levels of ZO-1 and P-cadherin and suppressed the increase in protein levels of desmin, FSP-1, Snail, and Slug in podocytes under high-glucose condition. Taken together, these results suggest the potential use of VASH-1 as a novel therapeutic agent in type 2 diabetic nephropathy mediated via antiangiogenic effects and maintenance of podocyte phenotype in association with antiproteinuric effects.

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