Optimization of Ultrasound-Assisted Extraction of Total Flavonoids from Dendranthema indicum var. aromaticum by Response Surface Methodology

Dendranthema indicum var. aromaticum is a new species with strong fragrance and is used as a herbal medicine by Chinese folks. The abundant flavonoids play important roles in its pharmacological activities. In this study, an ultrasound-assisted method was used to extract total flavonoids (TF) from D. indicum var. aromaticum by response surface methodology. A quadratic model was developed to optimize the extraction conditions, whose accuracy was verified by statistic analysis. Ethanol and acetic acid at the volume ratio of 70% : 2% were selected as the extract solvent. The optimized extraction conditions were as follows: extraction time, 40 min; solid/liquid ratio, 1 : 23 g/mL; and temperature, 60°C. This is the first report of an efficient and easy-operating method for TF extraction from D. indicum var. aromaticum. Besides, this study provides reference for future pharmacological research on D. indicum var. aromaticum and extraction of bioactive components from other herbs.

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Ultrasound-Assisted Extraction of Total Flavonoids from Corn Silk and Their Antioxidant Activity

Journal of Chemistry ◽

10.1155/2016/8768130 ◽

2016 ◽

Vol 2016 ◽

pp. 1-5 ◽

Cited By ~ 4

Author(s):

Ling-Li Zheng ◽

Guan Wen ◽

Min-Yong Yuan ◽

Feng Gao

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Antioxidant Activities ◽

Total Flavonoids ◽

Ultrasonic Power ◽

Ultrasound Assisted Extraction ◽

Antioxidant Power ◽

Corn Silk ◽

Assisted Extraction ◽

Ultrasound Assisted

Object. Ultrasound-assisted extraction of total flavonoids from corn silk and their antioxidant activities were studied.Methods. Response surface methodology was adopted to optimize the extraction conditions and antioxidant activities of the extracted total flavonoids were detected through ferric reducing antioxidant power (FRAP) assay.Results. Through a three-level, three-variable Box-Behnken design of response surface methodology (RSM) adopting yield as response, the optimal conditions were determined as follows: ultrasonic power 500 W, extraction time 20 min, material solvent ratio 1 : 20, and ethanol concentration 30%. Under the optimum conditions, the extraction yield of total flavonoids was 1.13%. FRAP value of total flavonoids extracted from corn silk was 467.59 μmol/L.Conclusion. The total flavonoids of corn silk could be developed as food natural antioxidant reagents.

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Process Optimization of Ultrasound-Assisted Extraction of Pectin from Helianthus annuus L. Heads by Response Surface Methodology

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.955-959.848 ◽

2014 ◽

Vol 955-959 ◽

pp. 848-854

Author(s):

Yin Xiang Gao ◽

Lei Yang ◽

Yuan Gang Zu ◽

Li Ping Yao

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Helianthus Annuus ◽

Response Surfaces ◽

Quadratic Model ◽

High Yield ◽

Extraction Temperature ◽

Ultrasound Assisted Extraction ◽

Assisted Extraction ◽

Ultrasound Assisted

An ultrasound-assisted procedure for the extraction of pectin from heads ofHelianthus annuusL. (sunflower) was established. A Box–Behnken design (BBD) was employed to optimize the extraction temperature (X1: 30–50°C), extraction time (X2: 20–40 min) and pH (X3: 2.5–3.5) to obtain a high yield of pectin with high degree of esterification (DE) from sunflower heads. Analysis of variance showed that the contribution of a quadratic model was significant for the pectin extraction yield and DE. An optimization study using response surface methodology was performed and 3D response surfaces were plotted from the mathematical model. According to the RSM model, the highest pectin yield (23.11 ± 0.08%) and DE (39.85 ± 0.14%) can be achieved when the UAE process is carried out at 50°C for 40min using a hydrochloric acid solution of pH 3.0. These results suggest that ultrasound-assisted extraction could be a good option for the extraction of functional pectin from sunflower heads at industrial level.

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Optimization of Ultrasound-Assisted Extraction Using Response Surface Methodology for Simultaneous Quantitation of Six Flavonoids in Flos Sophorae Immaturus and Antioxidant Activity

Molecules ◽

10.3390/molecules25081767 ◽

2020 ◽

Vol 25 (8) ◽

pp. 1767 ◽

Cited By ~ 2

Author(s):

Sanhong Fan ◽

Gege Yang ◽

Jinhua Zhang ◽

Jiani Li ◽

Baoqing Bai

Keyword(s):

Response Surface Methodology ◽

Free Radicals ◽

Response Surface ◽

Comprehensive Evaluation ◽

Antioxidant Activities ◽

Total Flavonoids ◽

Ultrasound Assisted Extraction ◽

Antioxidant Power ◽

Assisted Extraction ◽

Ultrasound Assisted

Ultrasound-assisted extraction (UAE) was applied to extract rutin (RU), nicotiflorin (NI), narcissoside (NA), kaempferol (KA), isorhamnetin (IS), quercetin (QU), and total flavonoids of Flos Sophorae Immaturus (TFFSI) from Flos Sophorae Immaturus (FSI). Through single factor test and response surface methodology (RSM), the optimal extraction conditions were concluded as follows: ethanol concentration 70%, time 30 min, temperature 61 °C, and liquid/solid ratio 15.30 mL/g, respectively. The actual extraction rates of RU, NI, NA, KA, IS, QU, and TFFSI were 14.6101%, 2.9310%, 7.1987%, 0.1041%, 0.4920%, 2.7998%, and 26.4260%, respectively. The experimental results demonstrated that the extraction method with accuracy and efficiency could be used for the comprehensive evaluation quality control of extracts from FSI. The antioxidant activities of hydroalcoholic extraction from FSI on 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+), superoxide anion (•O2−) free radicals, and ferric reducing/antioxidant power (FRAP) were assessed. The results showed that the antioxidation activities of extracts on DPPH, ABTS•+, and •O2− free radicals were reached 89.29%, 97.86%, and 56.61%, and 81.4% in FRAP at 1.0 mg/mL, respectively. The antioxidant capacity of FSI extract was positively correlated with the amount of total flavonoids.

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Optimization of Ultrasound-Assisted Extraction of Morphine from Capsules ofPapaver somniferumby Response Surface Methodology

International Journal of Analytical Chemistry ◽

10.1155/2015/796349 ◽

2015 ◽

Vol 2015 ◽

pp. 1-8 ◽

Cited By ~ 4

Author(s):

Ibrahim Bulduk ◽

Bahdışen Gezer ◽

Mustafa Cengiz

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Extraction Yield ◽

Quadratic Model ◽

Ultrasound Assisted Extraction ◽

Experimental Conditions ◽

Satisfactory Description ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasound Assisted

In this study, amount of morphine from poppy capsules(Papaver somniferum)was investigated using ultrasonic assisted extraction (UAE). Response surface methodology was used to estimate effective experimental conditions on the content extraction of poppy capsules. For this purpose, solvent/solid ratio (10–20 mL/500 mg sample), pH (1–13), time (30–60 min), and temperature (30–50°C) were chosen as experimental variables. The affected response is extraction recovery values for morphine from poppy straw. For interpreting the relationship between experimental factors and response, a design table was established with combinations of three different concentrations levels of this compound in 29 trials. The second order quadratic model gave a satisfactory description of the experimental data. In our study,R-Squared (0.96), Adj-R-Squared (0.92), and PredR-Squared (0.78) values for extraction yield display good accuracy of the derived model. The predicted optimal conditions for the highest morphine level (3.38 mg morphine/500 mg-sample) were found at 19.99 mL solvent/500 mg solid ratio, 59.94 min extraction time, 1.10 pH, and 42.36°C temperature. In the optimal extraction conditions, the experimental values are very close to the predicted values. Consequently, the response surface modeling can be achieved sufficiently to predict extraction yield from poppy straw by ultrasound assisted extraction.

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