Molecular Cloning and Expression Analysis of Interleukin-8 and -10 in Yellow Catfish and in Response to Bacterial Pathogen Infection

The yellow catfish (Pelteobagrus fulvidraco) is an important economic freshwater aquaculture species in Asia. However, little is known about its immune response to bacterial pathogen infection. Here, two cytokines, the proinflammatory cytokine interleukin-8 (IL-8) and the anti-inflammatory cytokine interleukin-10 (IL-10), were identified and characterized in the yellow catfish for the first time. We found that the full length of the IL-8 cDNA was 784 bp and contained an open reading frame (ORF) of 336 bp, while the IL-10 gene was 973 bp in length with a 549 bp of ORF. In addition, both the IL-8 and the IL-10 had similar tissue-specific expression patterns. They were more abundant in the spleen and lowest expressed in the liver. Furthermore, IL-10 but not IL-8 was significantly upregulated in the intestine of yellow catfish by feed supplementation ofClostridium butyricum(CB).More importantly, the expression levels of intestinal IL-10 and IL-8 were up- and downregulated by pathogenAeromonas punctatastimuli with the presence of CB, respectively. Collectively, these results suggest that IL-10 and IL-8 mediate important roles in the immunity of yellow catfish, and feed supplementation of CB may able to reduce the intestinal inflammation caused by bacteria infections through regulating the expression of IL-10 and IL-8.

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Gonadal transcriptomic analysis of yellow catfish (Pelteobagrus fulvidraco): identification of sex-related genes and genetic markers

Physiological Genomics ◽

10.1152/physiolgenomics.00088.2014 ◽

2014 ◽

Vol 46 (21) ◽

pp. 798-807 ◽

Cited By ~ 25

Author(s):

Jianguo Lu ◽

Peixian Luan ◽

Xiaofeng Zhang ◽

Shuqun Xue ◽

Lina Peng ◽

...

Keyword(s):

Sex Determination ◽

De Novo ◽

High Reliability ◽

Expression Patterns ◽

Pelteobagrus Fulvidraco ◽

Yellow Catfish ◽

Gonad Tissue ◽

Freshwater Aquaculture ◽

East And Southeast Asia ◽

Molecular Marker Development

Yellow catfish ( Pelteobagrus fulvidraco) has been recognized as a vital freshwater aquaculture species in East and Southeast Asia. In addition to its commercial interest, it is also attracted much attention because of its value in studying sex-determination mechanisms. A comprehensive gonadal transcriptome analysis is believed to provide a resource for genome annotation, candidate gene identification, and molecular marker development. Herein, we performed a de novo assembly of yellow catfish gonad transcriptome by high-throughput Illumina sequencing. A total of 82,123 contigs were obtained, ranging from 351 to 21,268 bp, and N50 of 2,329 bp. Unigenes of 21,869 in total were identified. Of these, 229 and 1,188 genes were found to be specifically expressed in XY gonad tissue for 1 yr and 2 yr old yellow catfish, respectively; correspondingly, 51 and 40 genes were identified in XX gonad tissue at those two stages. Gene ontology and KEGG analysis were conducted and classified all contigs into different categories. A large number of unigenes involved in sex determination were identified, as well as microsatellites and SNP variants. The expression patterns of sex-related genes were then validated by quantitative real-time PCR (qRT-PCR) suggesting the high reliability of RNA-Seq results. In this study, the transcriptome of yellow catfish gonad was first sequenced, assembled, and characterized; it provides a valuable genomic resource for better understanding of yellow catfish sex determination as well as development of molecular markers, thereby assisting in the production of monosex yellow catfish for aquaculture.

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Molecular cloning and expression pattern ofmyostatingene in yellow catfish (Pelteobagrus fulvidraco)

DNA Sequence ◽

10.1080/10425170701243492 ◽

2007 ◽

Vol 18 (4) ◽

pp. 279-287 ◽

Cited By ~ 16

Author(s):

Jianlin Pan ◽

Xiaolin Wang ◽

Wei Song ◽

Jianxiu Chen ◽

Caojun Li ◽

...

Keyword(s):

Molecular Cloning ◽

Expression Pattern ◽

Cloning And Expression ◽

Pelteobagrus Fulvidraco ◽

Yellow Catfish

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Cloning and expression analysis of c-type and g-type lysozymes in yellow catfish (Pelteobagrus fulvidraco)

Genes & Genomics ◽

10.1007/s13258-016-0414-6 ◽

2016 ◽

Vol 38 (8) ◽

pp. 707-716 ◽

Cited By ~ 1

Author(s):

Fei Ke ◽

Yun Wang ◽

XiaoYao Cao ◽

GaoWei Pan ◽

Rui Geng

Keyword(s):

Expression Analysis ◽

Cloning And Expression ◽

Pelteobagrus Fulvidraco ◽

Yellow Catfish

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Molecular cloning and expression analysis of interleukin-1β and interleukin-1 receptor type I genes in yellow catfish (Pelteobagrus fulvidraco): Responses to challenge of Edwardsiella ictaluri

Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology ◽

10.1016/j.cbpb.2018.05.001 ◽

2018 ◽

Vol 223 ◽

pp. 1-15 ◽

Cited By ~ 5

Author(s):

Chen-Lei Liao ◽

Gui-Rong Zhang ◽

Dong-Mei Zhu ◽

Wei Ji ◽

Ze-Chao Shi ◽

...

Keyword(s):

Molecular Cloning ◽

Expression Analysis ◽

Interleukin 1 ◽

Edwardsiella Ictaluri ◽

Cloning And Expression ◽

Receptor Type ◽

Interleukin 1Β ◽

Pelteobagrus Fulvidraco ◽

Type I ◽

Yellow Catfish

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cDNA cloning and expression of a xenobiotic metabolizing cytochrome P4501A (CYP1A) from the yellow catfish,Pelteobagrus fulvidraco (Siluriformes)

Environmental Toxicology ◽

10.1002/tox.20333 ◽

2008 ◽

Vol 23 (3) ◽

pp. 346-353 ◽

Cited By ~ 15

Author(s):

Jin-Hyoung Kim ◽

Dae-Sik Hwang ◽

Kyu-Hee Son ◽

Sheikh Raisuddin ◽

Jang-Seu Ki ◽

...

Keyword(s):

Cdna Cloning ◽

Cloning And Expression ◽

Pelteobagrus Fulvidraco ◽

Yellow Catfish ◽

Cytochrome P4501a

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Molecular cloning and expression pattern of 11 genes involved in lipid metabolism in yellow catfish Pelteobagrus fulvidraco

Gene ◽

10.1016/j.gene.2013.08.028 ◽

2013 ◽

Vol 531 (1) ◽

pp. 53-63 ◽

Cited By ~ 57

Author(s):

Jia-Lang Zheng ◽

Zhi Luo ◽

Qing-Ling Zhu ◽

Xiao-Ying Tan ◽

Qi-Liang Chen ◽

...

Keyword(s):

Lipid Metabolism ◽

Molecular Cloning ◽

Expression Pattern ◽

Cloning And Expression ◽

Pelteobagrus Fulvidraco ◽

Yellow Catfish

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Effects of Microplastics on Immune Responses of the Yellow Catfish Pelteobagrus fulvidraco Under Hypoxia

Frontiers in Physiology ◽

10.3389/fphys.2021.753999 ◽

2021 ◽

Vol 12 ◽

Author(s):

Li'ang Li ◽

Ran Xu ◽

Lingfeng Jiang ◽

Elvis Genbo Xu ◽

Man Wang ◽

...

Keyword(s):

Hypoxia Inducible Factor ◽

Aquatic Organisms ◽

Freshwater Ecosystem ◽

Pelteobagrus Fulvidraco ◽

Yellow Catfish ◽

High Concentration ◽

Negative Effects ◽

Freshwater Organisms ◽

Freshwater Aquaculture ◽

Tnf Α

Compared with marine organisms, research on microplastics (MPs) in freshwater organisms is still less although MPs have been widely found in the freshwater ecosystem. Hypoxia is a ubiquitous issue in freshwater aquaculture, and under such scenarios, the toxic effects of MPs on typical aquaculture fish need to be clarified. In this study, we studied the effects of MPs (polystyrene) on specific growth rate (SGR), hypoxia-inducible factor-1α (HIF-1α), tumor necrosis factor-α (TNF-α), interleukin-8 (IL-8), and interferon (IFN) in the yellow catfish (Pelteobagrus fulvidraco) under hypoxic conditions. After 15 days of exposure, the SGR was not affected by MPs or hypoxia. MPs significantly increased the expressions of HIF-1α and TNF-α but inhibited the expression of IFN at high concentration MPs under normoxia. However, hypoxia significantly inhibited the expression of IL-8 and TNF-α under high MP concentration and low MP concentration, respectively. In addition, MPs had significant concentration-dependent inhibitory effects on IFN under hypoxia. Surprisingly, a positive correction between HIF-1α and TNF-α was found in fish. Although hypoxia might alleviate the effects of MPs with low concentrations, the interaction of hypoxia and MPs aggravated the negative effects of MPs on immune factors at high concentration MPs. This study provided new insight into the complex effects of hypoxia and MPs on aquatic organisms, and future studies should focus on the cellular pathways of immune cells in fish. Given that MPs could induce the immune response in fish, considerations should be paid to the impacts of MPs on freshwater aquaculture, and hypoxia should be taken into consideration when evaluating the effects of MPs.

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