scholarly journals Effects of Dietary Protein Levels on Bamei Pig Intestinal Colony Compositional Traits

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Dong Wang ◽  
Guoshun Chen ◽  
Lili Song ◽  
Mingjie Chai ◽  
Yongfeng Wang ◽  
...  
Keyword(s):  
Dietary Protein ◽  
Protein Level ◽  
Crude Protein ◽  
Microbial Flora ◽  
Protein Levels ◽  
Low Protein ◽  
Dietary Crude Protein ◽  
Protein Diets ◽  
Crude Protein Level ◽  
Microbial Diversities

Diets containing different crude protein levels (16%, 14%, and 12%) were created to feed Bamei pigs in order to study the effect of these compositions on intestinal colonies. Therefore, 27 healthy Bamei pigs of similar weight ( 20.99   kg ± 0.16   kg ) were selected and randomly divided into three groups for microbial diversity analysis. The results of this study show that microbial diversities and abundances in Bamei pig jejunum and caecum samples after feeding with different dietary protein levels were significantly different. Dietary crude protein level exerted no significant effect on the Shannon index for cecum microbes in these pigs, while Simpson, ACE, and Chao1 indices for group I were all significantly higher than those of either the control group or group II ( P < 0.05 ). Indeed, data show that microbial diversities and abundances in the 14% protein level group were higher than those in either the 16% or 12% groups. Dominant bacteria present in jejunum and cecum samples given low-protein diets were members of the phyla Firmicutes and Bacteroidetes. Data show that as dietary crude protein level decreases, representatives of the microbial flora genus Lactobacillus in jejunum and cecum samples gradually increases. Values for the KEGG functional prediction of microbial flora at different dietary protein levels also show that genes of jejunum and cecum microorganisms were mainly enriched in the “metabolism” pathway and indicate that low protein diets increase intestinal metabolic activity. Therefore, we recommend that Bamei pig dietary protein levels are reduced 2% from their existing level of 16% crude protein. We also suggest that essential synthetic amino acids (AA) are added to optimize this ideal protein model as this will increase intestinal flora diversity in these pigs and enhance health. These changes will have a positive effect in promoting the healthy growth of Bamei pigs.

scholarly journals Low Crude Protein Diet Affects the Intestinal Microbiome and Metabolome Differently in Barrows and Gilts

2021 ◽  
Vol 12 ◽  
Author(s):  
Xin Tao ◽  
Bo Deng ◽  
Qizhi Yuan ◽  
Xiaoming Men ◽  
Jie Wu ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Dietary Protein ◽  
Crude Protein ◽  
High Protein Diet ◽  
Stage I ◽  
Protein Diet ◽  
Stage Ii ◽  
Protein Levels ◽  
Low Protein ◽  
Protein Diets

Low protein diets are commonly used in the growing-finishing pig stage of swine production; however, the effects of low dietary protein on the intestinal microbiota and their metabolites, and their association with pig sex, remain unclear. The present study aimed to assess the impact of a low crude protein (CP) diet on the gut microbiome and metabolome, and to reveal any relationship with sex. Barrows and gilts (both n = 24; initial body = 68.33 ± 0.881 kg) were allocated into two treatments according to sex. The four groups comprised two pairs of gilts and barrows fed with a high protein diet (CP 17% at stage I; CP 13% at stage II) and a low protein diet (CP 15% at stage I; CP 11% at stage II), respectively, for 51 d. Eight pigs in each group were slaughtered and their colon contents were collected. Intestinal microbiota and their metabolites were assessed using 16S rRNA sequencing and tandem mass spectrometry, respectively. The low protein diet increased intestinal microbiota species and richness indices (P &lt; 0.05) in both sexes compared with the high protein diet. The sample Shannon index was different (P &lt; 0.01) between barrows and gilts. At the genus level, unidentified Clostridiales (P &lt; 0.05), Neisseria (P &lt; 0.05), unidentified Prevotellaceae (P &lt; 0.01) and Gracilibacteria (P &lt; 0.05) were affected by dietary protein levels. The relative abundance of unidentified Prevotellaceae was different (P &lt; 0.01) between barrows and gilts. The influence of dietary protein levels on Neisseria (P &lt; 0.05), unidentified Prevotellaceae (P &lt; 0.01) and Gracilibacteria (P &lt; 0.05) were associated with sex. Metabolomic profiling indicated that dietary protein levels mainly affected intestinal metabolites in gilts rather than barrows. A total of 434 differentially abundant metabolites were identified in gilts fed the two protein diets. Correlation analysis identified that six differentially abundant microbiota communities were closely associated with twelve metabolites that were enriched for amino acids, inflammation, immune, and disease-related metabolic pathways. These results suggested that decreasing dietary protein contents changed the intestinal microbiota in growing-finishing pigs, which selectively affected the intestinal metabolite profiles in gilts.

scholarly journals Influence Of Crude Protein Diet on Growth Performance and Some Blood Biochemical Parameters of Growing Male Japanese Quail In Côte d’Ivoire

2021 ◽  
Vol 2 (1) ◽  
pp. 10-18
Author(s):  
Konan Kouassi Serge-Olivier ◽  
Bleyere Mathieu ◽  
Yves Bénarèce Tra Dje Bi ◽  
Soualio Kamagate ◽  
Paul Angoué
Keyword(s):  
Growth Performance ◽  
Japanese Quail ◽  
Dietary Protein ◽  
Protein Level ◽  
Crude Protein ◽  
Management Practices ◽  
Protein Levels ◽  
Blood Biochemical ◽  
Protein Group ◽  
Crude Protein Level

Purpose: This article aims to evaluate the effect of dietary protein levels on growth performance and some blood biochemical composition of male Japanese quail. Study Design: Qualitative Descriptive Design. Subjects and Methods: Three hundred, three week old apparently healthy male Japanese quail were used in this experiment. The birds were randomly allocated into 5 groups with 3 replicates of 20 birds for 21 days of experimental period and placed in cages, measuring 100 cm (length) x100 cm (width) x 80 cm (height). Group A was fed on diet 1 containing 18% crude protein, Group B was fed on diet 2 containing 20% crude protein, Group C was fed on diet 3 containing 22% crude protein, Group D was fed on diet 4 containing 24% crude protein and Group E was fed on diet 5containing 26% crude protein. All groups were subjected to similar management practices. Results: The findings showed that growth performance parameters significantly improved as dietary crude protein level increased. However dietary protein level beyond 22% has no beneficial effect on growth. Biochemical examination results demonstrated that the crude protein level in diet was not significantly affected the blood glucose, cholesterol,   bilirubin, calcium, phosphorus and liver enzyme activities (ASAT and ALT). However, we noticed a significant effect (P<0.05) about Total protein, Albumin, Urea, Triglycerides, Creatinine and Uric acid. Despite the variation of these parameters, they remain within the normal range. Conclusion: Therefore, it is concluded that dietary protein levels do not affect quail health. The crude protein requirement for Japanese quail from 21 to 42 days old is 22%.

The effect of low crude protein diets supplemented with DL-methionine and L-lysine hydrochloride on male broiler performance

2005 ◽  
Vol 2005 ◽  
pp. 165-165
Author(s):  
N. Eila ◽  
H. R. Semnani
Keyword(s):  
Amino Acids ◽  
Protein Level ◽  
Crude Protein ◽  
Essential Amino Acids ◽  
Broiler Chicks ◽  
Broiler Performance ◽  
Low Protein ◽  
Limiting Amino Acids ◽  
Protein Diets ◽  
Crude Protein Level

Crude protein is an expensive nutrient in broiler diets and the supply of essential amino acids is more important than crude protein. Therefore it’s possible to reduce crude protein level by supplementing diets with methionine and lysine as limiting amino acids. The object of this study was assaying low protein diets supplemented with DL-methionine and L-lysine hydrochloride on male broiler chicks.

scholarly journals The influence of phytase, pre-pellet cracked maize and dietary crude protein level on broiler performance via response surface methodology

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Amy F. Moss ◽  
Peter V. Chrystal ◽  
Yueming Dersjant-Li ◽  
Peter H. Selle ◽  
Sonia Yun Liu
Keyword(s):  
Weight Gain ◽  
Response Surface ◽  
Feed Intake ◽  
Protein Level ◽  
Crude Protein ◽  
Protein Diet ◽  
Dietary Crude Protein ◽  
Reduced Crude Protein ◽  
Protein Diets ◽  
Crude Protein Level

Abstract Background The reduction of crude protein levels in diets for broiler chickens may generate economic, environmental and flock welfare and health benefits; however, performance is usually compromised. Whole grain feeding and phytase may improve the utilization of reduced crude protein diets. Results The effects of pre-pellet cracked maize (0, 15% and 30%) and phytase (0, 750 and 1500 FTU/kg) in iso-energetic maize-soy diets with three levels of crude protein (22%, 19.5% and 17%) were evaluated via a Box-Behnken response surface design. Each of 13 dietary treatments were offered to 6 replicate cages (6 birds/cage) of male Ross 308 broiler chicks from 7 to 28 d post-hatch. Model prediction and response surface plots were generated from experimental data via polynomial regression in R and only significant coefficients were included and discussed in the predicted models. Weight gain, feed intake and FCR were all influenced by pre-pellet cracked maize, phytase and crude protein level, where crude protein level had the greatest influence. Consequently, the reduction from 22% to 17% dietary crude protein in non-supplemented diets reduced weight gain, feed intake, relative gizzard weight, relative gizzard content and relative pancreas weight but improved FCR. However, the inclusion of 30% cracked maize to 17% crude protein diets restored gizzard weight and 1500 FTU phytase inclusion to 17% crude protein diets increased relative gizzard contents and pancreas weights. Cracked maize and phytase inclusion in tandem to 17% crude protein diets increased weight gain, feed intake and FCR; however, this FCR was still more efficient than broilers offered the non-supplemented 22% crude protein diet. Broilers offered the pre-pellet cracked maize and phytase inclusions reduced AME in 22% crude protein diets but improved AME by 2.92 MJ (14.16 versus 11.24 MJ; P < 0.001) in diets containing 17% crude protein. Ileal N digestibility was greater in broilers offered diets with 17% crude protein than those offered the 22% crude protein diet; irrespective of phytase and pre-pellet cracked maize. Conclusion Pre-pellet cracked maize and phytase inclusions will improve the performance of broilers offered reduced crude protein diets.

scholarly journals Responses of milk urea nitrogen content to dietary crude protein level and degradability in lactatingHolsteindairy cows

2011 ◽  
Vol 51 (No. 12) ◽  
pp. 518-522
Author(s):  
Zhai Sw ◽  
Liu Jx ◽  
Wu Ym ◽  
Ye Ja ◽  
Xu Yn
Keyword(s):  
Milk Production ◽  
Milk Yield ◽  
Protein Level ◽  
Crude Protein ◽  
Latin Squares ◽  
Urea Nitrogen ◽  
Protein Levels ◽  
Dietary Crude Protein ◽  
Milk Urea ◽  
Crude Protein Level

&nbsp;Two experiments were conducted to investigate the effects of dietary crude protein level and degradability on milk urea nitrogen (MUN) content. In experiment 1, twelve multiparous lactating cows averaging 176 days in milk were divided according to DIM and milk production into three 4 &times; 4 Latin squares with four 2-week periods. Cows were fed four diets with different crude protein levels (13.0, 14.0, 15.0, and 16.0%, DM basis) with isocaloric, respectively. Crude protein levels had a low effect on milk yield and composition (P &gt; 0.05), but a significant effect on MUN content. There were significant differences in the MUN content of cows fed either of the two diets (P &lt; 0.01). In experiment 2, fifteen multiparous Holstein dairy cows averaging 91 days in milk were classified according to DIM and milk production into five 3 &times; 3 Latin squares with three 3-week periods. Cows were fed one of the three isocaloric and isonitrogenous diets with RUP being 30.8%, 36.2%, and 41.6% (CP basis), respectively. Milk yield, milk composition, and MUN content were not significantly affected by protein degradability, and there were no significant differences between any two dietary treatments (P &gt; 0.05). These results indicated that MUN might be used as a parameter to monitor the change in dietary protein levels. &nbsp;

Effect of castration and dietary protein level on growth and carcass composition in beef cattle

1970 ◽  
Vol 74 (2) ◽  
pp. 299-310 ◽  
Author(s):  
I. S. Robertson ◽  
H. Paver ◽  
J. C. Wilson
Keyword(s):  
Dietary Protein ◽  
Protein Level ◽  
Crude Protein ◽  
Dry Matter ◽  
Live Weight ◽  
Carcass Composition ◽  
Protein Levels ◽  
Dietary Protein Level ◽  
Dietary Crude Protein ◽  
Fixed Weight

SUMMARYThirty-six Hereford cross Friesian calves weighing on average 82.4 kg at an average age of 90 days were allotted in randomized blocks to a 2 x 3 factorial design. Castration by the open method was performed on half the number of calves and three dietary crude protein levels, 20, 14 and 11 % in dry matter were used in a mainly cereal concentrate diet fed ad libitum individually to the animals in stalls. Slaughter was at a fixed weight of approximately 420 kg.Steers compared with bulls gained weight at a significantly slower rate and with less efficiency. Castration's greatest effect in reducing rate of gain occurred during the later stages of rearing when puberty in the bulls, as measured by a steep rise in seminal fructose, was associated with their relatively well maintained live-weight gain. The influence of testicular hormones was also apparent in the bull's carcass with its heavier head, horn and hide and significantly higher proportion of lean and lower proportion of fat in the side.Dietary protein level affected rate of gain in both bulls and steers but at each level bulls performed better than steers. With circumstances similar to those in this experiment, a 14 % crude protein level in concentrate dry matter appears adequate for both categories up to a live weight of circa 270 kg and a reduction to 11 % thereafter. The effect of protein level on tissue proportions of the side and carcass composition generally was minimal.

scholarly journals Low Crude Protein Diet Affects the Intestinal Microbiome and Metabolome Differently in Barrows and Gilts

2021 ◽  
Author(s):  
Xin Tao ◽  
Bo Deng ◽  
Qizhi Yuan ◽  
Xiaoming Men ◽  
Jie Wu ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Dietary Protein ◽  
Crude Protein ◽  
Shannon Index ◽  
Protein Diet ◽  
Intestinal Microbiome ◽  
Protein Levels ◽  
Low Protein ◽  
The Impact ◽  
Protein Diets

Abstract Background Low protein diets are commonly used in the growing-finishing pig stage of swine production; however, the effects of low dietary protein on the intestinal microbiota and their metabolites, and their association with pig sex, remain unclear. The present study aimed to assess the impact of a low crude protein (CP) diet on the gut microbiome and metabolome, and to reveal any relationship with sex. Results Barrows and gilts (both n= 24; initial body = 68.33 ± 0.881 kg) were allocated into two treatments according to sex. The four groups comprised two pairs of gilts and barrows fed with a high protein diet (HPD, CP 17% at stage Ⅰ; CP 13% at stage Ⅱ) and a low protein diet (LPD, CP 15% at stage Ⅰ; CP 11% at stage Ⅱ), respectively, for 51 d. Eight pigs in each group were slaughtered and their colon contents were collected. Intestinal microbiota and their metabolites were assessed using 16S rRNA sequencing and tandem mass spectrometry, respectively. The LPD increased intestinal microbiota species and richness indices significantly in both sexes compared with the HPD. The Sample Shannon index was significantly different between barrows and gilts. At the phylum level, the LPD increased the relative abundance of Actinobacteria significantly. The influence of dietary protein levels on Proteobacteria and Synergistetes were associated significantly with sex. At the genus level, Clostridiales, Neisseria, and Prevotellaceae were affected significantly by dietary protein levels. In the latter two genera, the effects were significantly different between barrows and gilts. Metabolomic profiling indicated that dietary protein levels mainly affected intestinal metabolites in gilts rather than barrows. A total of 434 differently expressed metabolites were identified in gilts fed the two protein diets. Correlation analysis identified that six differentially abundant microbiota communities were closely associated with twelve metabolites that were enriched for amino acids, inflammation, immune, and disease-related metabolic pathways. Conclusions These results suggested that decreasing dietary protein contents benefitted the intestinal microbiota in growing-finishing pigs, which selectively affected the microbiota and metabolite profiles in gilts.

scholarly journals Effects of different dietary of protein and lipid levels on the growth of freshwater prawns (Macrobrachium carcinus) broodstock

2014 ◽  
pp. 3921-3929 ◽  
Author(s):  
Mario Benítez-Mandujano ◽  
Jesús T. Ponce-Palafox
Keyword(s):  
Body Composition ◽  
Dietary Protein ◽  
Protein Level ◽  
Crude Protein ◽  
The Body ◽  
Lipid Levels ◽  
Protein Levels ◽  
Dietary Protein Level ◽  
Dietary Crude Protein ◽  
Experimental Treatments

ABSTRACTObjective. Evaluate the effects of varying dietary protein and lipid levels on the growth and body composition of adult freshwater prawns, Macrobrachium carcinus (Linnaeus 1758), in a recirculation system for 11 weeks (77 days). Materials and methods. The experimental treatments were assigned in triplicate. Six test diets were formulated with three different protein levels (35, 40 and 45%) and two lipid levels (8 and 13%). Results. The highest survival rate, growth indices and feed utilization were observed for M. carcinus adults fed protein:lipid diets of 35:13, 40:13 and 45:13, and the lowest values for these parameters were recorded for prawns fed diets with the lowest lipid levels; the differences in these parameters between these types of diets were significant (p<0.05). A nonsignificant tendency for an increased percentage of protein in the body with an increased dietary protein level was observed. The percentage of lipids decreased with an increasing dietary protein level, and no definite trends in ash content were found. Conclusions. The results suggest that a diet with 35% dietary crude protein and 13% lipids enhances the growth and body composition of adult M. carcinus.

Effects of growth and dietary crude protein level until first insemination on milk production during first lactation in Holstein heifers

2011 ◽  
Vol 82 (6) ◽  
pp. 741-746 ◽  
Author(s):  
Takashige ISHII ◽  
Kenji KAWASHIMA ◽  
Haruo ORIBE ◽  
Hiromi UEDA ◽  
Toshiya HASUNUMA ◽  
...  
Keyword(s):  
Milk Production ◽  
Protein Level ◽  
Crude Protein ◽  
Dietary Crude Protein ◽  
Crude Protein Level

The performance of calves given concentrate diets treated with formaldehyde

1973 ◽  
Vol 24 (4) ◽  
pp. 613 ◽  
Author(s):  
GJ Faichney ◽  
HL Davies
Keyword(s):  
Food Consumption ◽  
Protein Level ◽  
Rumen Fluid ◽  
Amino Nitrogen ◽  
Peanut Meal ◽  
Plasma Urea ◽  
Protein Levels ◽  
Low Protein ◽  
Formaldehyde Treatment ◽  
Protein Diets

Five groups of Friesian bull calves were given concentrate diets containing 70 % barley in which low (12 %), medium (15 %), and high (19%) protein levels were obtained by varying the amount of peanut meal included. The effects of protein level and of formaldehyde treatment of the complete diet at the low and medium protein levels were studied in terms of liveweight gain, voluntary food consumption, digestibility of the diet, ammonia nitrogen in rumen fluid, and urea and a-amino nitrogen in blood plasma. Observations were begun when the calves reached 70 kg liveweight and continued until they reached 130 kg liveweight. The calves given the low protein diets grew more slowly than those given the higher protein diets. The calves given the high protein diet grew no better than those given the medium protein diets. Formaldehyde treatment was associated with an increase in the rate of liveweight gain of 9% (P = 0.11) at the low protein level but had practically no effect at the medium protein level. The treatment did not adversely affect voluntary food consumption but was associated with decreases in the digestibility of nitrogen and in rumen ammonia levels and small increases in plasma urea levels.

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