Sinusoidal Endothelial Cell Progenitor Cells Promote Tumour Progression in Patients with Hepatocellular Carcinoma

Objective. As sinusoidal endothelial cell progenitor cells (SEPCs) play a significant role in liver regeneration, it is necessary to elucidate whether SEPCs participate in tumour progression of hepatocellular carcinoma (HCC). Methods. A total of 45 patients with primary HCC who underwent liver resection were included in this study. The liver tumours were removed from the patients, and partial tissues were prepared to identify SEPCs through double staining of CD133/CD45 and CD133/CD31 at the same location. Blood samples were collected to examine liver function parameters and tumour markers. The demographics and clinicopathological characteristics of the patients were collected for correlation analysis with SEPCs. Results. SEPCs were observed in several blood vessels within the HCC nodules of all 45 patients, but no SEPCs were detected in the tumour-adjacent tissues. The number of SEPCs was correlated with the expression levels of HCC tumour markers α-fetoprotein (AFP) and CA199. There was a positive correlation between the expression of SEPC markers and diameter of HCC tumours in differently differentiated specimens ( P < 0.01 ). The expression levels of SEPC markers were significantly higher in patients with poorly differentiated HCC than in patients with moderately and highly differentiated HCC ( P < 0.05 ). Conclusions. SEPCs are closely associated with HCC progression; therefore, SEPCs may be considered potential prognostic and metastatic biomarkers and therapeutic candidates for HCC.

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Sinusoidal endothelial cell progenitor cells promote tumor progression in the patients with hepatocellular carcinoma.

10.21203/rs.3.rs-35602/v1 ◽

2020 ◽

Author(s):

Ya-xing Feng ◽

Wei Li ◽

Xu-dong Wen ◽

Ning Zhang ◽

Zhan-yu Yang ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Endothelial Cell ◽

Tumor Progression ◽

Progenitor Cells ◽

Tumor Markers ◽

Liver Tumors ◽

Sinusoidal Endothelial Cell ◽

Double Staining ◽

Pathological Characteristics ◽

Poorly Differentiated

Abstract Objective: Since sinusoidal endothelial cell progenitor cells (SEPCs) play great roles in liver regeneration, it is necessary to elucidate that whether these SEPCs participate in tumor progression of hepatocellular carcinoma (HCC).Methods: A total of 45 patients with primary HCC, who received liver resection, were included in this study. The liver tumors were removed from patients and partial tissues were prepared to identify SEPCs by double staining of CD133/CD45 and CD133/CD31 at the same location. Blood samples were correspondingly collected to examine liver function parameters and tumor markers. The demographics and clinic-pathological characteristics of the patients were all collected for correlation analysis with SEPCs.Results: SEPCs were observed within abundant of blood vessels in HCC nodules of all the 45 patients, but no SEPCs were detected in the tumor-adjacent tissues. The number of SEPCs was correlated with the expression levels of HCC tumor markers α-fetoprotein (AFP) and CA199. There was a positive correlation between the expressions of SEPCs markers and the diameters of HCC tumors in differently differentiated specimens (P<0.01). The expressions levels of SEPCs markers in poorly differentiated HCC patients were significantly higher than those in the moderately and highly differentiated HCC patients (P<0.05).Conclusions: SEPCs are in close connection with HCC progression, and they may potentially act as prognosis and metastasis predicting index and even therapeutic candidates of HCC.

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Expression and clinical significance of miR-3615 in hepatocellular carcinoma

Journal of International Medical Research ◽

10.1177/0300060520981547 ◽

2021 ◽

Vol 49 (1) ◽

pp. 030006052098154

Author(s):

Xin Yuan ◽

Yize Zhang ◽

Zujiang Yu

Keyword(s):

Hepatocellular Carcinoma ◽

Survival Curve ◽

Clinicopathological Characteristics ◽

The Cancer Genome Atlas ◽

Prognostic Information ◽

Ki 67 ◽

Tissue Samples ◽

Expression Levels ◽

Kaplan Meier ◽

Serum Alpha Fetoprotein

Objective To investigate the association between microRNA-3615 (miR-3615) expression and the prognosis and clinicopathological features in patients with hepatocellular carcinoma (HCC). Methods We obtained clinicopathological and genomic data and prognostic information on HCC patients from The Cancer Genome Atlas (TCGA) database. We then analyzed differences in miR-3615 expression levels between HCC and adjacent tissues using SPSS software, and examined the relationships between miR-3615 expression levels and clinicopathological characteristics. We also explored the influence of miR-3615 expression levels on the prognosis of HCC patients using Kaplan–Meier survival curve analysis. Results Based on data for 345 HCC and 50 adjacent normal tissue samples, expression levels of miR-3615 were significantly higher in HCC tissues compared with adjacent tissues. MiR-3615 expression levels in HCC patients were negatively correlated with overall survival time and positively correlated with high TNM stage, serum Ki-67 expression level, and serum alpha-fetoprotein level. There were no significant correlations between miR-3615 expression and age, sex, and pathological grade. Conclusion MiR-3615 may be a promising new biomarker and prognostic factor for HCC.

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High expression levels of putative hepatic stem/progenitor cells biomarkers related to tumor angiogenesis and poor prognosis of hepatocellular carcinoma

Journal of the American College of Surgeons ◽

10.1016/j.jamcollsurg.2009.06.220 ◽

2009 ◽

Vol 209 (3) ◽

pp. S89

Author(s):

Yang Xu ◽

Fan Jia ◽

Yang Xin-Rong ◽

Yu Bin

Keyword(s):

Hepatocellular Carcinoma ◽

Progenitor Cells ◽

Tumor Angiogenesis ◽

Poor Prognosis ◽

High Expression ◽

Expression Levels

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Rare primary hepatic carcinosarcoma composed of hepatocellular carcinoma, cholangiocarcinoma, and sarcoma: a case report

Journal of International Medical Research ◽

10.1177/03000605211050539 ◽

2021 ◽

Vol 49 (10) ◽

pp. 030006052110505

Author(s):

Ze Liang ◽

Jingzhao Han ◽

Hongfang Tuo ◽

Zepu Wang ◽

Yanhui Peng

Keyword(s):

Hepatocellular Carcinoma ◽

Malignant Lesion ◽

Clinicopathological Characteristics ◽

Spindle Cell Sarcoma ◽

Posterior Lobe ◽

Left Liver ◽

Poorly Differentiated ◽

Preoperative Magnetic Resonance ◽

Hepatic Carcinosarcoma ◽

The Right

Primary hepatic carcinosarcoma (HCS) is an extremely rare malignant tumor of the liver that contains carcinomatous and sarcomatous components. The diagnosis, treatment, and prognosis of HCS pose great challenges to clinicians. Herein, we present a case of HCS in a 67-year-old man with unique pathological manifestation. Preoperative magnetic resonance imaging showed a malignant lesion in the right liver and a small sub-focus in the left liver. Radical treatment was performed, including excision of the right posterior lobe of the liver, thrombectomy of the right posterior portal vein, and radiofrequency ablation of lesions in the left liver. The specimens were confirmed to be HCS by pathological examinations, which revealed a combination of poorly differentiated hepatocellular carcinoma, moderately differentiated cholangiocellular carcinoma, and spindle cell sarcoma. Transhepatic arterial chemotherapy and embolization was performed after surgery. Unfortunately, pulmonary metastasis occurred 1.5 months later, which meant a poor prognosis. In this report, we discuss the clinicopathological characteristics of this case and factors that affected surgical outcomes, which may add some ideas for the future diagnosis and treatment of HCS patients.

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High expression levels of putative hepatic stem/progenitor cells biomarkers related to tumor angiogenesis and poor prognosis of hepatocellular carcinoma

Journal of Clinical Oncology ◽

10.1200/jco.2009.27.15_suppl.e22121 ◽

2009 ◽

Vol 27 (15_suppl) ◽

pp. e22121-e22121

Author(s):

Y. Xu ◽

J. Fan ◽

X. Yang ◽

J. Zhou ◽

S. Qiu

Keyword(s):

Hepatocellular Carcinoma ◽

Progenitor Cells ◽

Tumor Angiogenesis ◽

Poor Prognosis ◽

High Expression ◽

Simplified Model ◽

Recurrence Time ◽

Cytokeratin 19 ◽

Expression Levels ◽

Qrt Pcr

e22121 Background: To investigate the prognostic values of putative hepatic stem/progenitor cells (HSCs/HPCs) biomarkers in hepatocellular carcinoma (HCC) patients. Methods: Fourteen biomarkers related with HSCs/HPCs or tumor angiogenesis were assessed by qRT-PCR and then validated by tissue microarrays (TMAs) in three independent cohorts of HCC patients underwent curative resection (n=67, 314 and 73). Results: Most of the biomarkers were found over-expressed in recurrent HCC patients by qRT-PCR. HSCs/HPCs biomarkers cytokeratin 19, ABCG2, CD133, Nestin, CD44 and angiogenesis agents CD34, VEGF and PD-ECGF, were confirmed as significant predictors for overall survival (OS) and/or relapse-free survival (RFS) in TMAs analysis. Compared with the low HSCs/HPCs profile group, patients with high HSCs/HPCs profile had significantly lower OS and RFS (p<0.0001), expressed higher VEGF levels (p = 0.012) and microvessel density (MVD, determined by CD34 immunostaining, p = 0.030). Based on Cox regression, a simplified model including CD133, CD44, Nestin, and MVD was constructed and confirmed as an independent predictor for OS (p<0.0001) and RFS (p<0.0001), regardless of alpha-fetoprotein level, tumor stage and recurrence time (p<0.0001 for all). Conclusions: High expression levels of HSCs/HPCs biomarkers are related to tumor angiogenesis and poor prognosis of HCC. The simplified model based on HSCs/HPCs and tumor angiogenesis profile can be used to classify HCC patients with high risk of tumor recurrence after operation. No significant financial relationships to disclose.

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