scholarly journals Identification and Validation of QTLs for Macronutrient Contents in Brown and Milled Rice Using Two Backcross Populations between Oryza sativa and O. rufipogon

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Biao-lin Hu ◽  
Xia Li ◽  
Ting Wu ◽  
De-run Huang ◽  
Feng-lin Huang ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Interspecific Cross ◽  
Public Health Issue ◽  
Milled Rice ◽  
Mineral Contents ◽  
Regulatory Pathways ◽  
Qtl Pyramiding ◽  
Backcross Inbred Lines ◽  
Backcross Populations ◽  
Dongxiang Wild Rice

Mineral malnutrition as a prevalent public health issue can be alleviated by increasing the intake of dietary minerals from major staple crops, such as rice. Identification of the gene responsible for mineral contents in rice would help breed cultivars enriched with minerals through marker-assisted selection. Two segregating populations of backcross inbred lines (BIL) were employed to map quantitative trait loci (QTLs) for macronutrient contents in brown and milled rice, BC1F5, and BC2F4:5 derived from an interspecific cross of Xieqingzao B (Oryza sativa) and Dongxiang wild rice (O. rufipogon). Phenotyping the populations was conducted in multiple locations and years, and up to 169 DNA markers were used for the genotyping. A total of 17 QTLs for P, K, Na, Ca, and Mg contents in brown and milled rice distributed on eight regions were identified in the BC1F5 population, which is explained to range from 5.98% to 56.80% of phenotypic variances. Two regions controlling qCa1.1 and qCa4.1 were validated, and seven new QTLs for Ca and Mg contents were identified in the BC2F4:5 population. 18 of 24 QTLs were clustered across seven chromosomal regions, indicating that different mineral accumulation might be involved in common regulatory pathways. Of 24 QTLs identified in two populations, 16 having favorable alleles were derived from O. rufipogon and 10 were novel. These results will not only help understand the molecular mechanism of macronutrient accumulation in rice but also provide candidate QTLs for further gene cloning and grain nutrient improvement through QTL pyramiding.

Genetic analysis and gene mapping of a rice few-tillering mutant in early backcross populations (Oryza sativa L.)

2001 ◽  
Vol 44 (6) ◽  
pp. 570-575 ◽  
Author(s):  
Jiabin Tang ◽  
Wanyong Zeng ◽  
Wenming Wang ◽  
Bingtian Ma ◽  
Yong Liu ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Genetic Analysis ◽  
Gene Mapping ◽  
Oryza Sativa L ◽  
Backcross Populations

QTL mapping of grain quality traits from the interspecific cross Oryza sativa × O. glaberrima

2004 ◽  
Vol 109 (3) ◽  
pp. 630-639 ◽  
Author(s):  
G. Aluko ◽  
C. Martinez ◽  
J. Tohme ◽  
C. Castano ◽  
C. Bergman ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Qtl Mapping ◽  
Grain Quality ◽  
Interspecific Cross ◽  
Quality Traits

scholarly journals Yield QTL analysis of Oryza sativa x O. glumaepatula introgression lines

2013 ◽  
Vol 48 (3) ◽  
pp. 280-286 ◽  
Author(s):  
Priscila Nascimento Rangel ◽  
Rosana Pereira Vianello ◽  
Arthur Tavares Oliveira Melo ◽  
Paulo Hideo Nakano Rangel ◽  
João Antônio Mendonça ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Interspecific Cross ◽  
Rice Genome ◽  
Introgression Lines ◽  
Wild Accession ◽  
Cultivated Rice ◽  
Two Generations ◽  
Ssr Loci ◽  
Donor Parent ◽  
Effect On Yield

The objective of this work was to evaluate the yield performance of two generations (BC2F2 and BC2F9) of introgression lines developed from the interspecific cross between Oryza sativa and O. glumaepatula, and to identify the SSR markers associated to yield. The wild accession RS‑16 (O. glumaepatula) was used as donor parent in the backcross with the high yielding cultivar Cica‑8 (O. sativa). A set of 114 BC2F1 introgression lines was genotyped with 141 polymorphic SSR loci distributed across the whole rice genome. Molecular analysis showed that in average 22% of the O. glumaepatula genome was introgressed into BC2F1 generation. Nine BC2F9 introgression lines had a significantly higher yield than the genitor Cica‑8, thus showing a positive genome interaction among cultivated rice and the wild O. glumaepatula. Seven QTL were identified in the overall BC2F2, with one marker interval (4879‑EST20) of great effect on yield. The alleles with positive effect on yield came from the cultivated parent Cica‑8.

scholarly journals Kualitas Fisik Beras (Oryza sativa L.) Mutan M4 Hasil Iradiasi Sinar Gamma

2020 ◽  
Vol 4 (1) ◽  
pp. 32-42
Author(s):  
Nanda Funna Ledita ◽  
Efendi Efendi ◽  
Rita Hayati
Keyword(s):  
Oryza Sativa ◽  
Gamma Ray ◽  
Amylose Content ◽  
Oryza Sativa L ◽  
Mutant Line ◽  
Milled Rice ◽  
Physical Quality ◽  
Significant Difference ◽  
Gamma Ray Irradiation

Abstrak.  Beras (Oryza sativa L.) merupakan salah satu tanaman pangan yang berfungsi sebagai tanaman pokok selain gandum dan jagung di Indonesia, dikarenakan mengandung nutrisi yang dibutuhkan tubuh seperti karbohidrat, protein, air  dan lemak. Meningkatnya jumlah penduduk maka permintaan terhadap beras semakin meningkat. Permintaan tersebut sangat ditentukan oleh selera konsumen yang dipengaruhi oleh kualitas beras yaitu mutu fisik (ukuran dan bentuk butiran, rendemen dan pengapuran), mutu tanak (kadar amilosa dan suhu gelatinisasi) dan mutu rasa (tekstur dan aroma). Penelitian ini bertujuan untuk mengetahui kualitas beberapa galur padi mutan M4 hasil iradiasi sinar gamma. Penelitian ini dilaksanakan di Laboratorium Ilmu dan Teknologi Benih, Laboratorium Analisis Pangan dan Hasil Pertanian, Laboratorium Ilmu Nutrisi dan Teknologi Pakan dan Laboratorium Fisiologi Tumbuhan Fakultas Pertanian Universitas Syiah Kuala, serta Laboratorium Departemen Ilmu dan Teknologi Pangan Fakultas Teknologi Pertanian Institut Pertanian Bogor, yang berlangsung pada bulan April hingga Juli 2018. Rancangan yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL) pola non faktorial dengan 3 ulangan. Perlakuan penelitian ini berupa galur (G) dengan 5 taraf, yaitu i-Snb (G0) kontrol, O-39e (G1), O-57d (G2), O-57e (G3) dan O-88j (G4). Parameter yang diamati yaitu sifat fisik beras (densitas kamba, derajat sosoh, dimensi beras rendemen beras giling, dan warna). Hasil penelitian menunjukkan bahwa galur  mutan M4 berpengaruh sangat nyata terhadap kualitas sifat fisik rendemen beras giling dan warna nilai L (kecerahan). Rendemen beras giling terbaik galur O-39e (70,33%) dan warna nilai L (kecerahan) galur O-88j (117,90).  Perlakuan terbaik secara kumulatif  untuk sifat fisik beras yaitu pada i-Snb.. Physical Quality of Rice (Oryza sativa L. ) M4  Mutant Results of Gamma Ray IrradiationAbstract. Rice (Oryza sativa L.) is one of main food which the function is to be staple food beside wheat and corn in Indonesia because it contains nutrients such as carbohydrates, protein, water, and fat. Towards the increasing populations, the demand of rice is increasing as well. The demand is largerly depends on the consumer taste affected by the quality of rice which are physical quality (size and grain shape, rendemen, and calcification), cooked quality (amylose content and gelatinization temperature) and flavor quality (texture and smell). The purpose of this research was to investigate the quality of rice from several M4 mutant line resulting from gamma ray irradiation. This research was conducted at the Laboratory of Seed Science and Technology and Laboratory of Food and Agricultural Analysis, Faculty of Agriculture, Syiah Kuala University, Banda Aceh, from the month April to July 2018. The design used in this study was based on a non-factorial completely randomize design with 3 replications and continued with the Least Significant Difference Test (LSD) level of 5% on a significant F test results. The results showed that the M4 mutant line had a very significant effect on the quality of physical properties, the yield of milled rice and the best color L (brightness), namely on the O-88j and O-39e line.

scholarly journals Mapping QTLs for cold tolerance at seedling stage using an Oryza sativa × O. rufipogon backcross inbred line population

2018 ◽  
Vol 54 (No. 2) ◽  
pp. 59-64 ◽  
Author(s):  
S. Yu ◽  
M. LI ◽  
Y. Xiao ◽  
D. Huang ◽  
D. Chen
Keyword(s):  
Oryza Sativa ◽  
Cold Tolerance ◽  
Inbred Line ◽  
Wild Rice ◽  
Chromosome 8 ◽  
Seedling Stage ◽  
Phenotypic Variance ◽  
Seedling Mortality ◽  
Dongxiang Wild Rice ◽  
Backcross Inbred Line

Tolerance to low temperature is an important factor affecting the growth and development of rice (Oryza sativa L.) at an early growing season in the temperate region, and at high altitudes of tropical regions. In this study, a backcross inbred line (BIL) population derived from an interspecific cross between Xieqingzao B (O. sativa L.) and an accession of Dongxiang wild rice (O. rufipogon Griff.) was used to identify quantitative trait loci (QTLs) associated with cold tolerance at the seedling stage. Seedlings were treated with a temperature of 6°C for 2 days and seedling mortality was measured for QTL mapping. QTL analysis was performed on the whole BIL population and on one subpopulation that showed Xieqingzao B homozygous at QTL detected in the whole population. One major QTL, qSCT8, and one QTL, qSCT4.3, with smaller effect was found in the whole population. The QTLs qSCT8 and qSCT4.3 were mapped on chromosome 8 and 4, explaining 60.96% and 8.83% of the phenotypic variance, respectively. In the subpopulation, three QTLs, qSCT4.1, qSCT4.2 and qSCT12, accounting for 56.22%, 57.62% and 53.09% of the phenotypic variance, respectively, were detected on chromosome 4 and 12. At all five loci, the alleles introduced from the Dongxiang wild rice were effective in decreasing seedling mortality. Our results provide a basis for fine mapping and cloning of QTLs associated with cold tolerance, and the markers linked with QTLs could be used to improve the cold tolerance of rice varieties by marker-assisted selection.

Large-scale density-based screening for pea weevil resistance in advanced backcross lines derived from cultivated field pea (Pisum sativum) and Pisum fulvum

2012 ◽  
Vol 63 (7) ◽  
pp. 612 ◽  
Author(s):  
N. Aryamanesh ◽  
O. Byrne ◽  
D. C. Hardie ◽  
T. Khan ◽  
K. H. M. Siddique ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Large Scale ◽  
Interspecific Cross ◽  
Field Pea ◽  
Separation Method ◽  
Density Separation ◽  
Advanced Backcross ◽  
Pisum Fulvum ◽  
Resistant Lines ◽  
Backcross Populations

The pea weevil, Bruchus pisorum, is one of the most intractable pest problems of cultivated field pea (Pisum sativum) in the world. Pesticide application, either as a contact insecticide spray to the field pea crop or fumigation of the harvested seed, is the only available method for its control. The aim of the study was to develop a quick and reliable method to screen for pea weevil resistance and increase efficiency in breeding for this important trait. Backcrossed progenies derived from an interspecific cross between cultivated field pea and its wild relative (Pisum fulvum, source of resistance for pea weevil) were subjected to natural infestation in field plots. Mature seeds were hand-harvested, stored to allow development of adult beetles, and then separated into infested and non-infested using a density separation method in 30% caesium chloride (CsCl). Susceptibility and resistance of the progenies were calculated based on this method and further confirmed by a glasshouse bioassay. Resistance in backcross populations improved considerably through selection of resistant lines using the density separation method. We found that the method using CsCl separation is a useful tool in breeding for pea weevil resistance. We were able to introgress pea weevil resistance from P. fulvum into cultivated field pea through backcrossing to produce several advanced pea weevil resistant lines following this procedure.

scholarly journals QTL analysis and dissection of panicle components in rice using advanced backcross populations derived from Oryza Sativa cultivars HR1128 and ‘Nipponbare’

PLoS ONE ◽  
2017 ◽  
Vol 12 (4) ◽  
pp. e0175692 ◽  
Author(s):  
Zhizhong Sun ◽  
Xiaoling Yin ◽  
Jia Ding ◽  
Dong Yu ◽  
Miao Hu ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Qtl Analysis ◽  
Advanced Backcross ◽  
Backcross Populations

S-nitrosocysteine-responsive genes modulate diverse regulatory pathways in Oryza sativa: a transcriptome profiling study

2018 ◽  
Vol 45 (6) ◽  
pp. 630 ◽  
Author(s):  
Bong-Gyu Mun ◽  
Sang-Uk Lee ◽  
Adil Hussain ◽  
Hyun-Ho Kim ◽  
Nkulu Kabange Rolly ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Oryza Sativa ◽  
Transcriptional Control ◽  
Nitrosative Stress ◽  
Genetic Model ◽  
Oryza Sativa L ◽  
Expression Patterns ◽  
Nitric Oxide Donor ◽  
Transcriptional Networks ◽  
Regulatory Pathways

Rice (Oryza sativa L.) is a major food crop and also a well-established genetic model. Nitric oxide (NO) and its derivatives are important signalling molecules that actively participate in various signalling pathways in response to different stresses. In this study, we performed RNA-seq mediated transcriptomic analysis of rice after treatment with the nitric oxide donor, S-nitroso-L-cysteine (CySNO), generating an average of 37.5 and 41.5 million reads from control and treated leaf samples respectively. More than 95% of the reads were successfully mapped to the O. sativa reference genome yielding a total of 33 539 differentially expressed genes (DEGs, P < 0.05). Further analyses identified 825 genes with at least 2-fold change in the expression following treatment with CySNO (P < 0.01). The DEGs identified were involved in diverse molecular functions such as catalytic activity, binding, transport, and receptor activity and were mostly located in the membrane, organelles such as nucleus, Golgi apparatus and mitochondria. DEGs also contained several genes that regulate responses to abiotic stresses such as drought, heat, cold and salt stress and biotic stresses. We also found significantly similar expression patterns of CySNO-responsive DEGs of rice with the CySNO-responsive DEGs of Arabidopsis in a previous study. Expression patterns of genes involved in key biological functions were verified using quantitative real time (qRT)-PCR. The findings of this study suggest that NO regulates the transcriptional control of genes involved in a wide variety of physiological functions in rice, and that NO-mediated transcriptional networks are highly conserved across the plant kingdom. This study provides useful information regarding the transcriptional response of plants to nitrosative stress.

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