scholarly journals Assessment of the Cytotoxic Activity of Triphala: A Semisolid Traditional Formulation on HepG2 Cancer Cell Line

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Ali Sahragard ◽  
Zohreh Alavi ◽  
Zohreh Abolhassanzadeh ◽  
Mahmoodreza Moein ◽  
Afshin Mohammadi-Bardbori ◽  
...  
Keyword(s):  
Natural Products ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Cancer Cell ◽  
Mtt Assay ◽  
Cancer Cell Line ◽  
Control Group ◽  
Anticancer Activities ◽  
Human Liver Cancer Cell ◽  
Human Liver Cancer

Cancer chemotherapies may result in resistance, and therefore, contemporary treatments including natural products may find an increasing consideration. As per Persian medicine (PM), many natural products have been used for malignant and chronic diseases. Triphala, with a combination of Terminalia chebula Retz., Terminalia bellirica Retz., Phyllanthus emblica L., and honey, is a multi-ingredient traditional formulation attributed to anticancer activities in PM. This study is aimed at evaluating the cytotoxic activity of this preparation on HepG2, the human liver cancer cell line. Hydroalcoholic extracts were prepared from the formulation and its components. Compared with the control and Cisplatin, the extracts were tested using MTT assay at different concentrations. All concentrations of the preparation, as well as Cisplatin, were effective significantly against HepG2 cells. All extract preparations at multiple concentrations were significantly effective as evidenced by MTT assay when compared to the control group. The IC50 level for Triphala extract was 77.63 ± 4.3   μ g / ml . Based on the results, Triphala and its components have cytotoxic activity on the HepG2 cancer cell line and they can reduce the survival rate significantly.

scholarly journals Apoptosis of human liver cancer cell line SMMC-7721 induced by Senjiu capsule

2003 ◽  
Vol 11 (7) ◽  
pp. 908-911
Author(s):  
Su-Dong Wu ◽  
Chang-Li Liu ◽  
Hui-Chuan Wang ◽  
De-Hu Bao
Keyword(s):  
Liver Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Human Liver ◽  
Cancer Cell Line ◽  
Liver Cancer Cell ◽  
Liver Cancer Cell Line ◽  
Human Liver Cancer Cell ◽  
Human Liver Cancer

scholarly journals Combination of Spices Aqueous Extracts as Antioxidant and Novel Anticancer Agents in Human Liver Cancer Cell Line

2016 ◽  
Vol 11 (1) ◽  
pp. 1-8
Author(s):  
Manal M. Ramadan ◽  
Amr F. Mansour ◽  
Reda M. Fekry ◽  
Marwa T. Salem ◽  
Fathy M. Mahaya ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Anticancer Agents ◽  
Human Liver ◽  
Cancer Cell Line ◽  
Aqueous Extracts ◽  
Liver Cancer Cell ◽  
Human Liver Cancer Cell ◽  
Human Liver Cancer

scholarly journals Phytochemical profiling and cytotoxic evaluation of Premna serratifolia L. against human liver cancer cell line

3 Biotech ◽  
2021 ◽  
Vol 11 (3) ◽  
Author(s):  
Chandrashekhar Singh ◽  
Sumit Kumar Anand ◽  
Kavindra Nath Tiwari ◽  
Sunil Kumar Mishra ◽  
Poonam Kakkar
Keyword(s):  
Liver Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Human Liver ◽  
Cancer Cell Line ◽  
Liver Cancer Cell ◽  
Liver Cancer Cell Line ◽  
Human Liver Cancer Cell ◽  
Human Liver Cancer

Cytotoxic and Apoptotic Effects of Novel Pyrrolo[2,3-d]Pyrimidine Derivatives Containing Urea Moieties on Cancer Cell Lines

2019 ◽  
Vol 18 (9) ◽  
pp. 1303-1312 ◽  
Author(s):  
Zühal Kilic-Kurt ◽  
Filiz Bakar-Ates ◽  
Bahriye Karakas ◽  
Özgür Kütük
Keyword(s):  
Cell Cycle ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Cancer Cell ◽  
A549 Cells ◽  
Cancer Cell Line ◽  
Anticancer Activities ◽  
Cycle Arrest ◽  
Apoptotic Protein ◽  
Mcf 7

Background: Pyrrolo[2,3-d]pyrimidines have been recently reported to have anticancer activities through inhibition of different targets such as, Epidermal Growth Factor Receptor (EGFR) tyrosine kinase, Janus Kinase (JAK), mitotic checkpoint protein kinase (Mps1), carbonic anhydrase, MDM-2. On the other hand, aryl urea moieties which are found in some tyrosine kinase inhibitors such as Sorafenib and Linifanib have aroused recent attention as responsible for anticancer activities. The aims of this paper are to synthesize pyrrolo[ 2,3-d]pyrimidine derivatives containing urea moiety and evaluate their anti-cancer activity against human lung cancer cell line (A549), prostate cancer cell line (PC3), human colon cancer cell line (SW480) and human breast cancer cell line (MCF-7). Methods: A series of new pyrrolo[2,3-d]pyrimidines containing urea moieties have been synthesized as Scheme 1. In vitro cytotoxicity of target compounds were evaluated against, SW480, PC3, A549 and MCF-7 human cancer cell lines using a MTT assay. In order to evaluate the mechanism of cytotoxic activity of compounds 9e, 10a and 10b, having the best cytotoxic activity, Annexin V binding assay, cell cycle analysis and western blot analysis were performed. Results: Among the target compounds, 10a (IC50 = 0.19 µM) was found to be the most potent derivative against PC3 cells. Compound 10b and 9e showed the strong cytotoxic activity against MCF-7 and A549 cells with IC50 value of 1.66 µM and 4.55 µM, respectively. Flow cytometry data suggest that the cytotoxic activity of the compounds on cancer cells might be mediated by apoptosis revealing a significant increase in the percentage of late apoptotic cells and causing a cell cycle arrest at different stages. Western blot analysis of apoptosis marker demonstrated that these compounds induce apoptosis through the intrinsic pathway. Conclusion: Compound 9e displayed the strongest cytotoxicity against A549 cancer cell line, and induced late apoptosis in A549, as confirmed by cell cycle arrest in G0/G1 phase. In addition, compound 9e reduced expression of the anti-apoptotic protein Bcl-2 and enhanced expression of the pro-apoptotic protein Bax, besides increased caspase-9 and caspase-3, as well as cleavage of PARP levels. These results suggest that compound 9e showed a cytotoxic effect in A549 cells through activation of the mitochondrial apoptotic pathway. Further studies will be undertaken in our laboratory to improve cytotoxic activity of compound 9e and to identify the biological targets of 9e which are responsible for anticancer activity.

Two variants of the human hepatocellular carcinoma-associatedHCAP1 gene and their effect on the growth of the human liver cancer cell line Hep3B

2003 ◽  
Vol 39 (1) ◽  
pp. 48-58 ◽  
Author(s):  
Dafang Wan ◽  
Mei He ◽  
Junru Wang ◽  
Xiaokun Qiu ◽  
Wei Zhou ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Human Liver ◽  
Cancer Cell Line ◽  
Liver Cancer Cell ◽  
Liver Cancer Cell Line ◽  
Human Liver Cancer Cell ◽  
Human Liver Cancer

PLGA nanoparticles containing α-fetoprotein siRNA induce apoptosis and enhance the cytotoxic effects of doxorubicin in human liver cancer cell line

2021 ◽  
Vol 553 ◽  
pp. 191-197
Author(s):  
Theeraphong Pho-iam ◽  
Primana Punnakitikashem ◽  
Chayapol Somboonyosdech ◽  
Sirinapa Sripinitchai ◽  
Patarabutr Masaratana ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Human Liver ◽  
Cancer Cell Line ◽  
Plga Nanoparticles ◽  
Liver Cancer Cell ◽  
Cytotoxic Effects ◽  
Human Liver Cancer Cell ◽  
Human Liver Cancer
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