Identifying PIF1 as a Potential Target of Wenxia Changfu Formula in Promoting Lung Cancer Cell Apoptosis: Bioinformatics Analysis and Biological Evidence

Lung cancer remains the leading cause of cancer-related deaths worldwide. Traditional Chinese medicine (TCM) is a valuable resource of active natural products and plays an important role in cancer treatment with the advantages of high efficiency and safety. Wenxia Changfu formula (WCF) is modified from Dahuang Fuzi decoction from Han Dynasty and has been used for treating lung cancer in China. Our previous research showed that WCF had an antitumor effect in vivo and in vitro, while the mechanism has not been well illustrated. In this study, the effect of WCF on the proliferative ability in three lung cancer cells and one noncancerous human cell line was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. WCF suppressed A549, H460, and PC-9 cell viability in a dose-dependent manner, with no inhibition of noncancerous MRC-5 cells after 48 h treatment with WCF (0–50 mg/mL). Furthermore, we screened for genes in A549 cells using four WCF-treated samples and four control samples on a gene expression profile microarray. 21 genes were significantly downregulated by WCF, which may potentially play an important role in the proliferation of A549 cells. High-content screening evaluated whether silencing the 21 genes affected A549 cell growth. The results showed that PIF1 knockdown exhibited the most potent inhibition of cell proliferation compared with the other genes. Downregulation of PIF1 increased A549 cell apoptosis and the activity of caspase 3/7. Besides, RT-PCR showed that the expression levels of PIF1 mRNA decreased significantly in A549, H460, and PC-9 cells after WCF treatment. In conclusion, the present observations indicate that WCF may inhibit lung cancer cell proliferation by promoting apoptosis via regulating the expression of PIF1.

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MiR-9-1 Suppresses Cell Proliferation and Promotes Apoptosis by Targeting UHRF1 in Lung Cancer

Technology in Cancer Research & Treatment ◽

10.1177/15330338211041191 ◽

2021 ◽

Vol 20 ◽

pp. 153303382110411

Author(s):

Cheng-You Jia ◽

Wei Xiang ◽

Ji-Bin Liu ◽

Geng-Xi Jiang ◽

Feng Sun ◽

...

Keyword(s):

Lung Cancer ◽

Cell Proliferation ◽

Cell Lines ◽

Cancer Cell ◽

Mrna Level ◽

A549 Cells ◽

Cancer Cell Lines ◽

G1 Arrest ◽

Lung Cancer Cell

Lung cancer is listed as the most common reason for cancer-related death all over the world despite diagnostic improvements and the development of chemotherapy and targeted therapies. MicroRNAs control both physiological and pathological processes including development and cancer. A microRNA-9 to 1 (miR-9 to 1) overexpression model in lung cancer cell lines was established and miR-9 to 1 was found to significantly suppress the proliferation rate in lung cancer cell lines, colony formation in vitro, and tumorigenicity in nude mice of A549 cells. Ubiquitin-like containing PHD and RING finger domains 1 (UHRF1) was then identified to direct target of miR-9 to 1. The inhibition of UHRF1 by miR-9 to 1 causes G1 arrest and p15, p16, and p21 were re-expressed in miR-9 to 1 group in mRNA level and protein level. Silence of UHRF1 expression in A549 cells resulted in the similar re-expression of p15, p16, p21 which is similar with miR-9 to 1 infection. Therefore, we concluded that UHRF1 is a new target for miR-9 to 1 to suppress cell proliferation by re-expression of tumor suppressors p15, p16, and p21 mediated by UHRF1.

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Plant-Derived Protease Inhibitors LC-pi (Lavatera cashmeriana) Inhibit Human Lung Cancer Cell Proliferation In Vitro

Nutrition and Cancer ◽

10.1080/01635581.2015.967876 ◽

2014 ◽

Vol 67 (1) ◽

pp. 156-166 ◽

Cited By ~ 3

Author(s):

Syed Rakashanda ◽

Asif Khurshid Qazi ◽

Rabiya Majeed ◽

Syed Mubashir Andrabi ◽

Abid Hamid ◽

...

Keyword(s):

Lung Cancer ◽

Cell Proliferation ◽

Protease Inhibitors ◽

Cancer Cell ◽

Human Lung ◽

Human Lung Cancer ◽

Cancer Cell Proliferation ◽

Lung Cancer Cell ◽

Proliferation In Vitro

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Bauhinia acuminata L. attenuates lung cancer cell proliferation: in vitro, in vivo and in silico approaches

Phytomedicine Plus ◽

10.1016/j.phyplu.2021.100173 ◽

2021 ◽

pp. 100173

Author(s):

Divya Sebastian ◽

K. Gowrishankar ◽

S. Ignacimuthu ◽

A.J. Renilda Sophy ◽

R. Vidhya ◽

...

Keyword(s):

Lung Cancer ◽

Cell Proliferation ◽

Cancer Cell ◽

In Silico ◽

Cancer Cell Proliferation ◽

Lung Cancer Cell ◽

Proliferation In Vitro

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Effects of lung cancer cell-associated B7-H1 on T-cell proliferation in vitro and in vivo

Brazilian Journal of Medical and Biological Research ◽

10.1590/1414-431x20165263 ◽

2016 ◽

Vol 49 (7) ◽

Author(s):

K. Chen ◽

H.T. Huang ◽

W.J. Hang ◽

L.B. Pan ◽

H.T. Ma

Keyword(s):

Lung Cancer ◽

Cell Proliferation ◽

T Cell ◽

Cancer Cell ◽

T Cell Proliferation ◽

Lung Cancer Cell ◽

Proliferation In Vitro

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EVALUATION OF IN VITRO CYTOTOXIC EFFECT OF VIOLACEIN PRODUCED BY NOVEL ISOLATE CHROMOBACTERIUM VACCINII CV5 AGAINST THE CERVICAL AND LUNG CANCER CELL

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i10.20456 ◽

2017 ◽

Vol 10 (10) ◽

pp. 227 ◽

Cited By ~ 1

Author(s):

Vishnu T Santhosh ◽

Palaniswamy Muthusamy

Keyword(s):

Lung Cancer ◽

Anticancer Activity ◽

Cell Lines ◽

Cancer Cell ◽

A549 Cells ◽

Dependent Manner ◽

Concentration Dependent Manner ◽

Cytotoxicity Activity ◽

Ic50 Values

Objectives: This study investigates the in vitro anticancer activity of the violacein extracted from the Chromobacterium vaccinii CV5.Methods: Natural colorants or dyes derived from flora to fauna are believed to be safe because of nontoxic, noncarcinogenic, and biodegradable in nature. There are a number of natural pigments, but only a few are available in sufficient quantities for industrial production. The cytotoxicity activity of pigment was assessed against the cervical (HeLa) and lung cancer (A549) cell lines using the MTT assay and there by potential cytotoxic activity exhibited by the pigment was identified.Results: The result of the pigment shows potent anticancer activity on the two cancer cell lines tested in a concentration dependent manner. The potent anticancer activity was observed with the pigment with IC50 values of 26 μg/mL on HeLa and 31 μg/mL on A549 cells, respectively.Conclusion: The study is pioneering report for determining the better in vitro anticancer activity of violacein from the novel isolate C. vaccinii CV5.

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