scholarly journals Effect of Erdosteine on Middle Ear Effusion in Rats by Mediating TLR4 Signaling Pathway

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Te Li ◽  
Wanting Zeng ◽  
Rongrong Liu
Keyword(s):  
Treatment Group ◽  
Signaling Pathway ◽  
Otitis Media ◽  
Middle Ear ◽  
Middle Ear Effusion ◽  
Model Group ◽  
Tlr4 Signaling ◽  
Expression Levels ◽  
Tlr4 Signaling Pathway ◽  
Ear Effusion

The study aimed to investigate the effect of erdosteine on middle ear effusion in rats through mediating the Toll-like receptor 4 (TLR4) signaling pathway. Rats were injected with endotoxin to prepare the model of acute secretory otitis media (SOM). Then, they were divided into an acute SOM model group (model group, n = 15 ) and erdosteine treatment group (18 mg/kg, gavage, treatment group, n = 15 ). Besides, a normal group ( n = 15 ) was set up. Two weeks later, routine biochemical indicators such as aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were detected. The inflammatory effusion due to otitis media was scored. The content of myeloperoxidase (MPO), matrix metalloproteinase (MMP), and tumor necrosis factor-beta (TNF-β) in middle ear lavage fluid was detected via enzyme-linked immunosorbent assay (ELISA). Additionally, histomorphological changes were observed with the help of hematoxylin-eosin (HE) staining, and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blotting assays were carried out to measure the expression levels of TLR4 pathway genes and proteins as well as the messenger ribonucleic acid (mRNA) expression levels of key factors for otitis media (mucin 2 (MUC2) and MUC5A). In the model group, the levels of AST, ALP, and glutamic-pyruvic transaminase (GPT) were significantly increased ( p < 0.05 ). Besides, the content of MPO, MMP, and TNF-β was overtly raised in the model group ( p < 0.05 ), while it was notably lowered in the treatment group ( p < 0.05 ). In the treatment group, the cilia were slightly swollen, and inflammatory cells were fewer. The mRNA levels of MUC2, MUC5A, and pathway genes TLR4 and c-Jun N-terminal kinase (JNK) were elevated in the model group. In addition, the protein assay results revealed that the protein levels of TLR4 and JNK were evidently increased in the model group. Erdosteine can treat the middle ear effusion in rats by repressing the activation of the TLR4 signaling pathway.

Lack of Efficacy of Middle-Ear Inflation: Treatment of Otitis Media with Effusion in Children

1989 ◽  
Vol 100 (4) ◽  
pp. 317-323 ◽  
Author(s):  
Kenny H. Chan ◽  
Charles D. Bluestone
Keyword(s):  
Randomized Controlled Trial ◽  
Treatment Group ◽  
Otitis Media ◽  
Middle Ear ◽  
Otitis Media With Effusion ◽  
Controlled Trial ◽  
Observation Group ◽  
Middle Ear Effusion ◽  
Randomized Controlled ◽  
Ear Effusion

A randomized controlled trial was conducted to evaluate the efficacy of autoinflation of the middle ear in the treatment of otitis media with effusion. Forty-one children with middle ear effusion not responsive to antimicrobial therapy enrolled in the study. Of these subjects, 75 percent had effusion duration over 3 months. A system consisting of a disposable anesthesia mask attached to a flowmeter was used to teach children to perform autoinflation based on a modified Valsalva technique. Subjects were stratified according to their ability to achieve tubal opening as assessed by tympanometry and tubosonometry. Subjects were randomly assigned to either treatment or observation group and were followed weekly for 2 weeks; subjects in the treatment group attempted inflation three times each day for 2 weeks. Of the 19 subjects who were in the autoinflation group, only one (5.3 percent) was effusion-free at the two-week endpoint and of the 21 subjects in the control, only two (9.5 percent) were without middle-ear effusion. Autoinflation was ineffective for treatment of children with otitis media with effusion in our study.

Antibacterial Therapy and Middle Ear Effusions

1976 ◽  
Vol 85 (2_suppl) ◽  
pp. 250-253 ◽  
Author(s):  
Paul A. Shurin
Keyword(s):  
Drug Therapy ◽  
Effective Treatment ◽  
Otitis Media ◽  
Middle Ear ◽  
Middle Ear Effusion ◽  
Prolonged Administration ◽  
Antimicrobial Drugs ◽  
Acute Attacks ◽  
Ear Effusion

Antimicrobial drugs chosen for their activity against the causative pathogens of otitis media provide effective treatment for acute attacks. Prolonged administration of some of these agents has recently been shown to be of value in the prevention of symptomatic otitis. The role of drug therapy in the management of chronic or recurrent middle ear effusion is unknown at present.

scholarly journals The management of acute otitis media

1984 ◽  
Vol 22 (14) ◽  
pp. 53-54
Keyword(s):  
Hearing Loss ◽  
Otitis Media ◽  
Clinical Examination ◽  
Middle Ear ◽  
Acute Otitis Media ◽  
Infectious Complications ◽  
Middle Ear Effusion ◽  
Suppurative Otitis Media ◽  
Painful Condition ◽  
Ear Effusion

Acute suppurative otitis media (AOM) is a common, painful condition affecting 20% of children under 4 years at least once a year,1 and perhaps more in infancy when clinical examination is most difficult. Infectious complications such as mastoiditis, meningitis and cerebral abscess are now rare, but chronic middle ear effusion and hearing loss remain common. Hearing loss may persist long after the infective episode,2 and may impair learning.

To Tube or Not to Tube: That Continues to Be the Question

PEDIATRICS ◽  
1985 ◽  
Vol 76 (2) ◽  
pp. 332-332
Author(s):  
DAVID W. TEELE ◽  
BERNARD ROSNER ◽  
JEROME O. KLEIN
Keyword(s):  
Otitis Media ◽  
Middle Ear ◽  
Otitis Media With Effusion ◽  
Middle Ear Effusion ◽  
Speech And Language ◽  
Clinically Significant ◽  
Ear Effusion

In Reply.— We appreciate Chamberlin's thoughtful comments about the associations between otitis media with effusion and development of speech and language and his concern about results that are statistically significant but may not be "clinically significant." We share his view that measurements of the sequelae of disease should include those that are meaningful to the child. We reported results of tests of speech and language that indicated that children who had spent many days with middle ear effusion during the first years of life scored significantly lower than did children who had not spent such time.

Role of Tonsillectomy and Adenoidectomy in the Management of Children with Middle Ear Effusion

1980 ◽  
Vol 89 (5_suppl) ◽  
pp. 43-46 ◽  
Author(s):  
James B. Snow
Keyword(s):  
Otitis Media ◽  
Middle Ear ◽  
Clinical Effectiveness ◽  
First Year ◽  
Middle Ear Effusion ◽  
Beneficial Effects ◽  
Per Se ◽  
Second Year ◽  
Ear Effusion

Evidence for the clinical effectiveness of adenoidectomy or tonsillectomy and adenoidectomy in the management of children with persistent middle ear effusions is incomplete and what evidence there is in the literature is not convincing. In the first year after tonsillectomy and adenoidectomy there is less otitis media. The beneficial effects of tonsillectomy and adenoidectomy on the incidence of otitis media declines in the second year after the operation. The prevailing opinion is that adenoidectomy is not indicated in the vast majority of children in whom a middle ear effusion has persisted for six weeks or more in spite of antibiotic therapy. Factors favoring an adenoidectomy would be persistent nasal obstruction, persistent purulent rhinorrhea and persistent adenoiditis. The role of tonsillectomy is even less clear, but the decision regarding tonsillectomy is made by most clinicians on the basis of the amount of intercurrent or chronic tonsillitis rather than on the basis of the middle ear effusion per se.

Efficacy of medical treatment as an adjunct to surgery in the treatment of secretory otitis media

1986 ◽  
Vol 100 (12) ◽  
pp. 1347-1350 ◽  
Author(s):  
T. H. J. Lesser ◽  
M. I. Clayton ◽  
D. Skinner
Keyword(s):  
Medical Treatment ◽  
Otitis Media ◽  
Middle Ear ◽  
Otitis Media With Effusion ◽  
Randomised Trial ◽  
Control Group ◽  
Middle Ear Effusion ◽  
Secretory Otitis Media ◽  
Ear Effusion

AbstractIn a pilot controlled randomised trial of 38 children who had bilateral secretory otitis media, with effusion demonstrated at operation, we compared the efficacy of a six-week course of an oral decongestant—antihistamine combination and a mucolytic preparation with a control group in preventing the presence of middle-ear effusion six weeks after myringotomy and adenoidectomy. The mucolytic preparation decreased the presence of middle-ear effusion when compared to the decongestant-antihistamine combination and the control group (p=0.06).

scholarly journals Experimental Otitis Media With Effusion Induced by Middle Ear Effusion

1992 ◽  
Vol 102 (9) ◽  
pp. 1037???1042 ◽  
Author(s):  
Junko Nakata ◽  
Masashi Suzuki ◽  
Hideyuki Kawauchi ◽  
Goro Mogi
Keyword(s):  
Otitis Media ◽  
Middle Ear ◽  
Otitis Media With Effusion ◽  
Middle Ear Effusion ◽  
Ear Effusion

Experimental Otitis Media with Effusion following Middle Ear Inoculation of Nonviable H Influenzae

1984 ◽  
Vol 93 (1) ◽  
pp. 52-56 ◽  
Author(s):  
Thomas F. DeMaria ◽  
David J. Lim ◽  
Bruce R. Briggs ◽  
Nobuhiro Okazaki
Keyword(s):  
Otitis Media ◽  
Middle Ear ◽  
Animal Experiment ◽  
Otitis Media With Effusion ◽  
Type B ◽  
Negative Bacterium ◽  
Middle Ear Effusion ◽  
Sterile Saline ◽  
Inflammatory Changes ◽  
Ear Effusion

In order to test the hypothesis that nonviable bacteria can induce middle ear inflammation leading to persistent middle ear effusion (MEE), we conducted an animal experiment using formalin-killed Hemophilus influenzae, the bacterium reported to be the most common pathogen isolated from chronic MEEs. Over 70% of the chinchillas injected with formalin-killed H influenzae type b or a nontypeable isolate developed sterile, straw-colored serous MEEs, and exhibited histological evidence of extensive inflammatory changes of the middle ear mucosal connective tissue and epithelium. Control animals injected with pyrogen-free sterile saline did not exhibit any inflammatory changes or effusions in the middle ears. Our data suggest that endotoxin on the surface of H influenzae, a gram-negative bacterium, may be responsible for the induction of the otitis media with effusion. It is suggested that endotoxin (even when the organisms are no longer viable) may be responsible for the production of serous MEE and inflammatory changes in the middle ear.

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