scholarly journals Domestic Exposure to Fungi and Total Serum IgE Levels in Asthmatic Children

2005 ◽  
Vol 2005 (3) ◽  
pp. 167-170 ◽  
Author(s):  
Huey-Jen Jenny Su ◽  
Pei-Chih Wu ◽  
Huan-Yao Lei ◽  
Jiu-Yao Wang
Keyword(s):  
Airborne Fungi ◽  
Total Serum ◽  
Dose Response Relationship ◽  
Total Ige ◽  
Serum Ige ◽  
Asthmatic Children ◽  
Specific Serum ◽  
Dust Mite ◽  
Der P1 ◽  
Dust Mite Allergen

We measured the number of airborne, viable fungi and house dust mite (HDM) allergen levels in the homes of a group of asthmatic children. Blood samples were drawn and the amounts of total and specific serum IgE were determined. The association between the number of fungal colonies, dust mite allergen exposure, and specific and total IgE was evaluated. The number of viable airborne fungi was high (20 543 CFU/m) in those investigated houses.Der p1concentrations on child's mattress exceeding 2μg/g were found in 78.6% of the houses. A quantitative dose-response relationship was demonstrated between the exposure to viable, airborne molds and the amount of total IgE (r=0.4399andP=.0249) and the level was further increased in children with coexposure to viable fungi and HDM.

scholarly journals House Dust Mite Allergen Levels of Der p1 and Der f1 in Houses of Asthmatic Children

2020 ◽  
Vol 8 (4) ◽  
pp. 267-274
Author(s):  
Abbas Dabbaghzadeh ◽  
◽  
Javad Ghaffari ◽  
Mohammad Feridoni ◽  
Afrooz Alipour ◽  
...  
Keyword(s):  
House Dust ◽  
House Dust Mite ◽  
Childhood Asthma ◽  
Mite Allergen ◽  
House Dust Mites ◽  
House Dust Mite Allergen ◽  
Asthmatic Children ◽  
Dust Mite ◽  
Der P1 ◽  
Dust Mite Allergen

Background: Exposure to house dust mites is an important cause of asthma among children. The main asthma-causing mites found in homes are Dermatophagoides farinae and Dermatophagoides. pteronyssinus. Der f1 or Der p1 are allergens of the mentioned mites. This study aimed to assess the levels of Der p1, and Der f1 in the homes of families that had a case of childhood asthma in a northern city of Iran and to determine the association between indoor house environment and the level of mites allergens present there.  Objectives: This study aimed to evaluate House Dust Mite Allergen Levels of Der p1 and Der f1 in Houses of Asthmatic Children.  Methods: Dust samples were collected from bedrooms of 91 homes of families that had cases of childhood asthma. Families filled a questionnaire about demographical characteristics and the indoor condition of their living environment. The levels of Der f1 and Der p1 were measured by enzyme-linked immunosorbent assay (ELISA). Statistical analysis was performed in SPSS v. 24 and a P-value less than 0.05 was considered significant. Results: we found that Der p1 was more prevalent than Der f1 among samples. The mean concentration of Der p1 concentration was 271.35 ng/g (9 times greater than the concentration of Der f1). When the indoor environment status was evaluated, it was found that keeping pets and having old carpets are associated with Der f1 (P<0.05). However, no significant association was found between Der p1 and the indoor conditions of the home. Conclusions: Our study showed that Der p1 level is higher than Der f1 level in the houses of children with asthma. We suggest removing carpets and pets from the house. 

Age-dependent relationship between bronchial hyperresponsiveness to methacholine and total serum IgE level in asthmatic children

2001 ◽  
Vol 87 (1) ◽  
pp. 33-38 ◽  
Author(s):  
Miyuki Kono ◽  
Hiroyuki Mochizuki ◽  
Hirokazu Arakawa ◽  
Masahiko Kato ◽  
Kenichi Tokuyama ◽  
...  
Keyword(s):  
Bronchial Hyperresponsiveness ◽  
Total Serum ◽  
Serum Ige ◽  
Asthmatic Children ◽  
Dependent Relationship ◽  
Age Dependent

scholarly journals Enhanced Expression of IL-18 and IL-18BP in Plasma of Patients with Eczema: Altered Expression of IL-18BP and IL-18 Receptor on Mast Cells

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Yalin Hu ◽  
Junling Wang ◽  
Huiyun Zhang ◽  
Hua Xie ◽  
Weiwei Song ◽  
...  
Keyword(s):  
Mast Cells ◽  
Fold Increase ◽  
Flow Cytometry Analysis ◽  
Altered Expression ◽  
House Dust Mite Allergen ◽  
Dust Mite ◽  
Enhanced Expression ◽  
Der P1 ◽  
Dust Mite Allergen

IL-18 has been found to be associated with eczema. However, little is known of the role of IL-18 binding protein (BP) and IL-18 receptor (R) in eczema. We therefore investigated the expression of IL-18, IL-18BP, and IL-18R on mast cells by using flow cytometry analysis and mouse eczema model. The results showed that plasma free IL-18 and free IL-18BP levels in eczema patients were higher than those in healthy controls. IL-18 provoked up to 3.1-fold increase in skin mast cells. IL-18 induced also an increase in IL-18BP+ mast cells, but a reduction of IL-18R+ mast cells in mouse eczema skin. It was found that house dust mite allergen Der p1 and egg allergen OVA induced upregulation of the expression of IL-18, IL-18BP, and IL-18R mRNAs in HMC-1 cells following 2 and 16 h incubation. In conclusion, correlation of IL-18 and IL-18BP in eczema plasma suggests an important balance between IL-18 and IL-18BP in eczema. The decrease in molar concentration ratio of plasma IL-18BP/IL-18 and allergen-induced upregulated expression of IL-18 and IL-18R in skin mast cells of the patients with eczema suggests that anti-IL-18 including IL-18BP therapy may be useful for the treatment of eczema.

scholarly journals The role of rs_7927894 FLG gene polymorphism and total IgE in predicting clinical phenotypes of atopic dermatitis in children

2021 ◽  
pp. 31-36
Author(s):  
V.O. Dityatkovsky ◽  
◽  
O.E. Abaturov ◽  
N.V. Naumenko ◽  
O.O. Alifirenko ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Total Serum ◽  
Gene Variant ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Developmental Risk ◽  
Total Ige ◽  
Serum Ige ◽  
Clinical Phenotypes ◽  
Serum Total Ige

Atopic dermatitis (AD) is the most common chronic allergic disease of childhood, the pathogenesis of which is based on endogenous genotype and which manifests by various clinical phenotypes — isolated or combined with other forms of atopy — allergic rhinitis/rhinoconjunctivitis (AR/ARC) and/or bronchial asthma (BA). Currently, one of the most studied genetic markers of AD developmental risk is the single nucleotide polymorphism of the filaggrin gene (SNP FLG), in particular, rs_7927894. The basic AD biomarker is total serum immunoglobulin E (IgE). But, so far, there has been no studies combining the mentioned predictors markers within different clinical AD phenotypes in children. Purpose — to detect the variants of SNP rs_7927894 of FLG gene and serum total IgE concentrations as personalized predictors panel for different AD clinical phenotypes developmental risk in children. Materials and methods. There were recruited 2 groups of patients into the study: the main comprised 39 children with phenotypes of AD isolated and combined with AR/ARC and/or BA; the control group comprised 47 children with disorders of digestive system (functional dyspepsia, chronic gastritis, peptic ulcer, functional biliary disorders) without clinical signs of atopy. The threshold level of statistical significance was set as p<0.05. Results. There were detected the predictor genotype and biomarker for the AD developmental risk as per AD isolated phenotype: 4.11 (95% CI 1.28; 13.18, p<0.05) within C/T SNP rs_7927894 of FLG gene variant and 8.98 (95% CI 2.53, 31.86, p<0.001) for total serum IgE>173 IU/ml. As well, predictor genotype and biomarker for the developmental risk of the AD combined with AR/ARC/BA phenotype were detected: 2.88 (95% 1.07; 8.54, p<0.05) within the C/T SNP rs_7927894 of FLG gene variant and 8.98 (95% CI 2.53; 31.86, p<0.001) for total serum IgE>213 IU/ml. Additionally, the developmental risk for the phenotype of AD combined with AR/ARC/ BA in comparison with AD isolated at a cut-off serum total IgE>1001 IU/ml was detected as 16.00 (95% CI 2.68; 95.44, p<0.01). Conclusions. The C/T SNP rs_7927894 of FLG gene variant and cut(off serum IgE concentrations are significantly associated with the developmental risk of AD clinical phenotypes in children. Total IgE remains a significant predictor biomarker of AD risk in children aged 3 to 18 years at serum concentrations >173 IU/ml for the AD isolated and at serum concentrations >213 IU/ml for the AD combined with AR/ARC/AD phenotypes. The level of total serum IgE>1001 IU/ml is a significant predictor biomarker for the developmental risk of AD phenotype combined with AR/ARC/BA in comparison to the AD isolated phenotype in children. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of all participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interest was declared by the authors. Key words: atopic dermatitis, children, phenotype, filaggrin gene, single(nucleotide polymorphism, total immune globulin E.

scholarly journals Total IgE Distribution in Food Allergy Suspected Patients in Republic of Macedonia (2001-2011)

2015 ◽  
Vol 3 (2) ◽  
pp. 202-208
Author(s):  
Slavica Hristomanova Mitkovska ◽  
Dejan Trajkov ◽  
Aleksandar Petlichkovski ◽  
Olivija Efinska-Mladenovska ◽  
Mirko Spiroski
Keyword(s):  
Food Allergy ◽  
Human Genetics ◽  
Specific Ige ◽  
Average Concentration ◽  
Total Serum ◽  
Age Group ◽  
Total Ige ◽  
Serum Ige ◽  
Number Of Patients

BACKGROUND: IgE may be considered the hallmark of allergic disorders. It is easily detected in serum and can be measured as total IgE and as allergen-specific IgE. In fact, the serum IgE assay is used to diagnose an allergy.AIM: The aim of this study is to evaluate, investigate and present the distribution of total serum IgE levels, determined with UniCap system, in food-allergy suspected patients in a Republic of Macedonia.MATERIAL AND METHODS: In this study we analyzed retrospectively 8898 consecutive patients that were admitted for allergy testing at the Institute of Immunobiology and Human Genetics during the ten year period between 01.01.2001 and 01.01.2011. Total IgE levels in patient sera were detected with the in vitro system UniCAP100 (Pharmacia, Uppsala, Sweden).RESULTS: When we analyzed the number of patients according to the total IgE groups, we noted that most of the patients have normal levels of total IgE in serum. However, we also discovered a group of patients with elevated levels of total IgE that are greater than 200 kU/L. The average concentration of total serum IgE is higher in women in the age group 6 (6-7 years), followed by a steep decrease in the age group 9 (9-10 years), and after that the average concentrations of total IgE were mostly constant with the exception of a partial increase in the age group 21 (65-69 years). For men, the average serum concentrations of total IgE were highest in the age group of 6 (6-7 years), which was significantly higher than the average concentrations of total IgE in all other age groups.CONCLUSION: The large number of enrolled patients, a particular strength of this study, revealed that average concentrations of total IgE in men are higher than in women and that total IgE did not decrease with age. On the contrary, increased total IgE levels were found in patients aged 65 and 69 of both genders. We continue our work with analyses of the specific IgE antibodies values toward food and the correlation with total IgE values.

scholarly journals Campylobacter jejuni infection and IgE sensitization in up to 2-year-old infants

2015 ◽  
Vol 72 (2) ◽  
pp. 140-147 ◽  
Author(s):  
Dara Jovanovic ◽  
Nevenka Ilic ◽  
Biljana Miljkovic-Selimovic ◽  
Dragoljub Djokic ◽  
Tijana Relic ◽  
...  
Keyword(s):  
High Frequency ◽  
Case Control Study ◽  
Total Serum ◽  
Serum Concentrations ◽  
Atopic Diseases ◽  
Total Ige ◽  
Serum Ige ◽  
Campylobacter Infection ◽  
Enzyme Test ◽  
Control Study

Background/Aim. The ?hygiene hypothesis? addresses the correlation between the occurrence of atopy and the frequency of infections in the earliest age, explaining an increase in the incidence of atopic diseases by living in good, infection-free, hygienic conditions. The aim of our study was to determine the conection between atopy and Campylobacter infection, and to analyze the association between serum concentrations of total IgE and Campylobacter infection in relation to atopy in children up to two years. Methods. A case control study was conducted with the sample of 98 infants of the average age of 8 months. Total serum IgE and Phadiatop infant multi-test were determined on Immunocap-100 (Phadia AB, Uppsala, Sweden). The presence of atopy was determined by detection of serumspecific IgE ? 0.35 kUA/L (Phadiatop infant positive) and serum IgM, IgA, IgG levels against C. jejuni were determined by a quantitative immuno-enzyme test - SERION ELISA classic. Results. Total IgE cut-off values ? 15 kU/L point to atopy in infants, and tIgE cut-off values ? 8.1 kU/L pointed to a C. jejuni infection in infants. Within the group of atopic children, tIgE levels ? 29.8 kU/L point to C. jejuni infection, and within the group of non-atopic children, tIgE levels ? 5.9 kU/L point to infection. Enteritis is not a predictor of C. jejuni infection, because of a high frequency of asymptomatic cases of infection. The risk factors for C. jejuni infection are age and tIgE, and the protective factors are breastfeeding and atopy. Conclusion. C. jejuni infection increases the total serum IgE level, which is predictive of infection, regardless of the presence of atopy. The presence of symptomatic C. jejuni infection reduces the risk of atopy in a child of the age of 5-24 months by the factor of 10.

MO276TOTAL AND SPECIFIC IGE IN PATIENTS WITH IGA NEPHROPATHY

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
Darya Nizheharodava ◽  
Kirill Komissarov ◽  
Victoria Shadrina ◽  
Alena Minchenko ◽  
V Pilotovich ◽  
...  
Keyword(s):  
Iga Nephropathy ◽  
Positive Reaction ◽  
Specific Ige ◽  
Total Serum ◽  
Dermatophagoides Farinae ◽  
Crescent Formation ◽  
Female Ratio ◽  
Total Ige ◽  
Dust Mite ◽  
Male Female Ratio

Abstract Background and Aims IgA nephropathy (IgAN) is the most common form of primary glomerulonephritis characterized by impaired immunological tolerance. One of possible trigger factors for IgAN initiation and development are allergens which have been described to form IgA complexes deposited in the mesangium. Previous studies reported that serum immunoglobulin class E (IgE) levels are elevated and suggested as a prognostic indicator in glomerular diseases. However, mechanisms of IgE/IgG4-mediated allergic reactions involvement in IgAN as well as specific allergens and their clinical significance have poorly been investigated. The aim of the study was to assess the total serum IgE and IgG4 level and to determine the allergoprofile in IgAN patients. Method The peripheral blood was obtained from 21 IgAN patients (aged of 32.0 (27.0 ÷ 36.0) y.o., male/female ratio as 14/7) and 18 donors (aged of 38.0 (30.0 ÷ 46.0) y.o., male/female ratio as 10/8). IgAN diagnosis was confirmed in patients’ biopsy materials according to the Oxford classification (MEST-C score). The concentrations of total IgE and IgG4 were determined using «IgE total-ELISA-BEST» kit («Vector-Best», RF) and «Human IgG4 Platinum ELISA» kit («eBioscience», Austria)». Allergen-specific IgE to 55 inhalation (domestic, epidermal, fungal, plant) or food allergens were measured using «EUROLINE Atopy Screen IgE» immunoblot kit («Euroimmun», Germany). Statistical analysis was done in Statistica 8.0. Results The increased level of total IgE (in 47,4% of cases) but not IgG4 was established in sera of IgAN patients as compared to donors (p=0.004). Moreover, proteinuria was lower in IgAN patients with a high IgE level as compared to normal one (610 (220 ÷ 910) mg/day vs 1545 (600 ÷ 2200) mg/day, p=0.01) as well as there were no any crescent formation. The investigation of allergen-specific IgE in IgAN patients revealed a strong positive reaction to dust mite allergens of Dermatophagoides pter. (22,75 (16,72 ÷ 35,69) IU/ml – in 29% of cases) and Dermatophagoides farinae (38,24 (12,19 ÷ 65,88) IU/ml – in 43% of cases) correlated with the total IgE (R=0,57, p=0,03) and IgM (R=0,54, p=0,04). The more concentration of specific IgE to dust mite allergens was the lower the severity of segmental glomerulosclerosis (R= -0.55, p=0.04), crescent formation (R= -0.56, p=0.03), proteinuria (R= -0.65, p=0.01) and haematuria (R = -0.55, p = 0.04) were reported. A weak positive reaction was established to other domestic (house dust, honey bee venom, common wasp venom, cockroach), epidermal (cat), plant (sweet vernal grass, orchard grass, timothy grass, cultivated rye, alder, birch, hazel, oak, common ragweed, mugwort, plantain) and food (carrot, sesame, hazelnut, apple) allergens in 9–18% of IgAN patients. Conclusion The prevailing of specific IgE to dust mite allergens of Dermatophagoides pter. and Dermatophagoides farinae but not to food or plant allergens was determined in IgAN patients. The obtained data demonstrated that IgAN pathogenesis in patients with latent sensibilization to dust mite allergens is characterized by a milder disease course what allows to identify this cohort as possible secondary form of IgAN.

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