Abstract 4643: A novel 3-biomarker quantitative protein assay predicts survival in stage I lung squamous cell carcinomas

e22010 Background: Tumor hipoxia induces the up-regulation of several genes via the hipoxia-inducible transcription factors (HIF) 1 and 2. HIF-2 alpha (HIF-2 α) and HIF-1 alpha (HIF-1α) are associated with the prognosis of operable NSCLC patients. We studied the immunohistochemical expression of HIF-1α and HIF-2 α in patients with NSCLC and the possible correlation with the maximum standardised uptake value (max SUV) of 18F-FDG as well as other biological parameters. Methods: We used a Tissue Arrayer device (Beecher Instruments. WI) to construct a TMA block, according to conventional protocols for the study of immunohistochemical expression of HIF-1α, HIF-2 α, EGFR, bcl-2, MIB1, p16, p63 and cyclins A, B1, D1 and D3. Sections were scored as positive if >10% of cells stained positively. Staining patterns were correlated to clinical variables. Results: HIF- 1α expression was observed in 84/96 patients (34/39 adenocarcinomas and 50/57 squamous cell carcinomas), however it did not correlate with clinical stage (I-II: 41/45 vs III-IV: 44/51). HIF-1α correlated positively with HIF-2 α (p:0,001) and EGFR (p:<0,001) expressions. The maxSUV values of 18F-FDG-PET were higher (p:0,039) in HIF-1α -positive (17,1±8,6) than in negative tumors (11,8±4,4). HIF-2 α expression was observed in 60/103 cases (27/42 adenocarcinomas and 33/61 squamous cell carcinomas) and it did not correlate with clinical stage ((I-II: 28/45 vs III-IV: 32/57). HIF-2 α correlated positively with HIF-1α (p:0,001), MIB1 (p:0,045) and EGFR (p:0,091) expressions. After multivariate analysis, only the clinical stage (RR: 2,2) was a prognostic factor. Conclusions: 1) HIF-1α and HIF-2 α expressions are frequent in patients with NSCLCs and it did not correlate with clinical stage; 2) maxSUVs FDG-PET values were higher in HIF1alpha positive than in HIF-1α negative patients; 3) HIF-1α was correlated with EGFR expression, while HIF-2 α was correlated with MIB1 expression. No significant financial relationships to disclose.

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Cytological study of stage I and II squamous cell carcinomas in the oral cavity.

Journal of Japanese Society of Oral Oncology ◽

10.5843/jsot.5.284 ◽

1993 ◽

Vol 5 (3) ◽

pp. 284-289

Author(s):

Mutsumi Uchiyama ◽

Tadamitsu Kameyama ◽

Schunichi Tanaka ◽

Jingo Kusukawa ◽

Tamaka Okina ◽

...

Keyword(s):

Oral Cavity ◽

Squamous Cell ◽

Cytological Study ◽

Squamous Cell Carcinomas ◽

Stage I

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Correlation between Skp2 expression and nodal metastasis in Stage I and II oral squamous cell carcinomas

Oral Diseases ◽

10.1111/j.1601-0825.2010.01713.x ◽

2010 ◽

Vol 17 (1) ◽

pp. 102-108 ◽

Cited By ~ 11

Author(s):

P Tosco ◽

G M La Terra Maggiore ◽

P Forni ◽

S Berrone ◽

L Chiusa ◽

...

Keyword(s):

Squamous Cell ◽

Nodal Metastasis ◽

Squamous Cell Carcinomas ◽

Stage I ◽

Oral Squamous Cell Carcinomas ◽

Skp2 Expression

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Comparison of the seventh and eighth editions of the American Joint Committee on Cancer (AJCC) staging for oropharyngeal squamous cell carcinomas (OPSCC): A Surveillance, Epidemiology and End Results Program (SEER) database analysis.

Journal of Clinical Oncology ◽

10.1200/jco.2019.37.15_suppl.e17538 ◽

2019 ◽

Vol 37 (15_suppl) ◽

pp. e17538-e17538

Author(s):

Sumita Trivedi ◽

Haocan Song ◽

Yuan Liu ◽

Conor Ernst Steuer ◽

William Stokes ◽

...

Keyword(s):

Overall Survival ◽

Head And Neck ◽

Squamous Cell ◽

Disease Free Survival ◽

Squamous Cell Carcinomas ◽

Stage I ◽

Seer Database ◽

Free Survival ◽

Ajcc Staging ◽

Disease Free

e17538 Background: The recently released eighth edition of the American Joint Committee on Cancer (AJCC) Staging Manual, Head and Neck Section, incorporates significant changes to the prior seventh edition. The changes reflect the improved understanding of tumor biology, prognostic factors and molecular markers that effect outcomes in Head and Neck cancers. A key update restages OPSCC by human papilloma virus (HPV) positive and negative cancers as data demonstrated that these tumors have significantly different biology and outcomes. Methods: Using SEER data from 2004 – 2014, we identified male patients with squamous cell carcinomas of the tonsil, base of tongue and soft palate aged between 21 and 64 years old (those clinical characterizes were considered as surrogate markers for HPV positive status). We classified them by the AJCC 8th edition staging for HPV positive OPSCC and by AJCC 7th edition staging. The prediction performance by two staging editions were compared regarding overall survival (OS) and Disease free survival (DFS). Kaplan-Meier method and Cox proportional hazard model were applied, and the discrimination performance was measured by the concordance statistics (C-statistics). Results: A total of 8202 eligible patients were included in the analysis with a median follow up period of 51 months. 7415 (90.4%) patients had previously received radiation and 7038 (85.8%) patients had previously received chemotherapy. The median age of patients was 56 years. Distribution of stage I disease increased from 2% to 19.6% in AJCC 8th edition. 10-year overall survival (OS) for AJCC 8th stages I (74%), II (78%), III (55%) and IV (32%). Using Stage I as reference, the hazard ratio for stage II, III, and IV is 0.98 (95%CI: 0.87-1.09), 2.29 (95%CI: 2.04-2.57), and 5.88 (95%CI: 4.96-6.98). Similar results were noted for ten year disease free survival. The C-statistics measured overall discrimination for 8th edition is 0.68 and 0.63 for the 7th edition (P < 0.001). Conclusions: Based on this SEER analysis, the overall performance of discrimination improved from AJCC 7th to 8th edition; but in this study population, AJCC 8th edition does not distinguish stage I and II sufficiently as expected as it does for stages III and IV disease. Limitations of the SEER database include the surrogate for P16 status and under reported and incomplete data.

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