Abstract B31: Single-cell multiplex immunofluorescence of formalin-fixed, paraffin-embedded prostate cancer tissue identifies PI3K pathway activation in two prospective cohort studies

AbstractExtraction of DNA and RNA from formalin-fixed paraffin-embedded (FFPE) tissue blocks is a critical process in molecular oncology testing. Using FFPE, it is possible to choose the portion of tissue to study, taking into account the cell morphology, storage stability and storage conditions at room temperature, and make retrospective studies with clinical and pathological information. In prostate cancer tissue, in contrast with macroscopic tumors, it is not easy to identify the tumor; therefore, it is very important to make a microscopic diagnosis. We do not recommend punching this tissue because it can choose normal tissue for molecular analysis. In the present article we review the differences between punch biopsy and microdissection.

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Multiplex Immunofluorescence in Formalin-Fixed Paraffin-Embedded Tumor Tissue to Identify Single-Cell–Level PI3K Pathway Activation

Clinical Cancer Research ◽

10.1158/1078-0432.ccr-20-2000 ◽

2020 ◽

Vol 26 (22) ◽

pp. 5903-5913

Author(s):

Konrad H. Stopsack ◽

Ying Huang ◽

Svitlana Tyekucheva ◽

Travis A. Gerke ◽

Clyde Bango ◽

...

Keyword(s):

Single Cell ◽

Tumor Tissue ◽

Pi3k Pathway ◽

Single Cell Level ◽

Cell Level ◽

Pathway Activation ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Formalin Fixed

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Proteomics Analysis of Formalin Fixed Paraffin Embedded Tissues in the Investigation of Prostate Cancer

Journal of Proteome Research ◽

10.1021/acs.jproteome.9b00587 ◽

2019 ◽

Vol 19 (7) ◽

pp. 2631-2642 ◽

Cited By ~ 2

Author(s):

Anna Mantsiou ◽

Manousos Makridakis ◽

Konstantinos Fasoulakis ◽

Ioannis Katafigiotis ◽

Constantinos A. Constantinides ◽

...

Keyword(s):

Prostate Cancer ◽

Proteomics Analysis ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Formalin Fixed

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Expression of E-cadherin in prostate cancer in formalin-fixed, paraffin-embedded tissues: correlation with pathological features

Pathology ◽

10.1080/00313020220131282 ◽

2002 ◽

Vol 34 (3) ◽

pp. 233-238 ◽

Cited By ~ 27

Author(s):

İsmail Türker Köksal ◽

Faruk Özcan ◽

Işin Kiliçaslan ◽

Ahmet Tefekli

Keyword(s):

Prostate Cancer ◽

Pathological Features ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Formalin Fixed ◽

E Cadherin

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Molecular Markers for Prostate Cancer in Formalin-Fixed Paraffin-Embedded Tissues

BioMed Research International ◽

10.1155/2013/283635 ◽

2013 ◽

Vol 2013 ◽

pp. 1-15 ◽

Cited By ~ 9

Author(s):

Tamara Sequeiros ◽

Marta García ◽

Melania Montes ◽

Mireia Oliván ◽

Marina Rigau ◽

...

Keyword(s):

Prostate Cancer ◽

Molecular Markers ◽

Tumor Grade ◽

Developed Countries ◽

Response To Therapy ◽

Tissue Samples ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Ffpe Tissues ◽

Formalin Fixed

Prostate cancer (PCa) is the most frequently diagnosed type of cancer in developed countries. The decisive method of diagnosis is based on the results of biopsies, morphologically evaluated to determine the presence or absence of cancer. Although this approach leads to a confident diagnosis in most cases, it can be improved by using the molecular markers present in the tissue. Both miRNAs and proteins are considered excellent candidates for biomarkers in formalin-fixed paraffin-embedded (FFPE) tissues, due to their stability over long periods of time. In the last few years, a concerted effort has been made to develop the necessary tools for their reliable measurement in these types of samples. Furthermore, the use of these kinds of markers may also help in establishing tumor grade and aggressiveness, as well as predicting the possible outcomes in each particular case for the different treatments available. This would aid clinicians in the decision-making process. In this review, we attempt to summarize and discuss the potential use of microRNA and protein profiles in FFPE tissue samples as markers to better predict PCa diagnosis, progression, and response to therapy.

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Performance of Automated Dissection on Formalin-Fixed Paraffin-Embedded Tissue Sections for the 21-Gene Recurrence Score Assay

Technology in Cancer Research & Treatment ◽

10.1177/1533033820960760 ◽

2020 ◽

Vol 19 ◽

pp. 153303382096076

Author(s):

Peng Qi ◽

Qian-ming Bai ◽

Qian-lan Yao ◽

Wen-tao Yang ◽

Xiao-yan Zhou

Keyword(s):

Ductal Carcinoma ◽

Recurrence Score ◽

Cancer Tissue ◽

Tissue Block ◽

Tissue Sections ◽

Epithelial Tissues ◽

Formalin Fixed Paraffin ◽

Gene Assay ◽

Formalin Fixed Paraffin Embedded ◽

Formalin Fixed

This study aimed to compare the performance of MilliSect dissection and manual dissection. Twenty-five formalin-fixed paraffin-embedded (FFPE) breast cancer tissue blocks were selected for comparison. Specific areas of interest (AOIs) in invasive carcinoma on tissue sections were transferred to dissection slides by manual macrodissection or the MilliSect instrument. The comparison criteria were 1) the time required for dissection; 2) RNA concentration and purity; 3) RNA quantity of 5 housekeeping genes (by RT-qPCR); and 4) ER, PR, HER2, Ki-67 and recurrence score (RS) values (by the 21-gene assay). Then, tumor-adjacent tissues, including fibrocollagenous and epithelial tissues, from the same selected tissue blocks of 8 of 25 patients were scraped using the mesodissection method, and their RS values were assessed to evaluate the influence of tumor-adjacent tissues on the target AOIs. Ultimately, 4 AOIs of invasive ductal carcinoma (IDC) from 1 tissue block of another 4 patients with lymph node (LN) metastases each, LN tissue and a mixture of IDC and LN tissue from the other tissue block of the same 4 patients were mesodissected to evaluate the influence of infiltrating lymphocyte levels on the RS values of AOIs. In our experience, the MilliSect instrument, which provides process management documentation, required more time than manual macrodissection (on average, approximately 9.1 min per sample versus 5.8 min per sample, respectively). The RNA yield and quality of the dissected tissues were comparable for the 2 methods. However, the tumor-adjacent tissues of the AOIs may influence the RS to some extent. Tumor-infiltrating lymphocytes (TILs) can dramatically increase RSs, far exceeding the influence of tumor-adjacent fibrocollagenous and epithelial tissues. In conclusion, MilliSect mesodissection is comparable to manual dissection. This mesodissection tool may facilitate AOI alignment and the dissection process for the 21-gene RS assay. Samples whose adjacent tissues are intermixed with TILs warrant special attention.

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