Effect of Acupuncture Treatment on Uterine Motility and Cyclooxygenase-2 Expression in Pregnant Rats

2000 ◽  
Vol 50 (4) ◽  
pp. 225-230 ◽  
Author(s):  
Jeong-sang Kim ◽  
Ki Hyoung Shin ◽  
Chang Su Na
Keyword(s):  
Acupuncture Treatment ◽  
Cyclooxygenase 2 ◽  
Pregnant Rats

Immunohistochemical Localization of Cyclooxygenase-2 in Pregnant Rat Uterus by Sp-6 Acupuncture

2003 ◽  
Vol 31 (03) ◽  
pp. 481-488 ◽  
Author(s):  
Jeong-Sang Kim ◽  
Chang Su Na ◽  
Woo Jun Hwang ◽  
Byung Chul Lee ◽  
Ki Hyoung Shin ◽  
...  
Keyword(s):  
Acupuncture Treatment ◽  
Immunohistochemical Localization ◽  
Cyclooxygenase 2 ◽  
Uterine Contractility ◽  
Rat Uterus ◽  
Pregnant Rats ◽  
Pregnant Rat ◽  
Complementary Method ◽  
Key Factor ◽  
Cox 2

As pregnancy advances, prostaglandins (PG) increase in the uterus, leading to elevated uterine contractility. Therefore, regulating the concentration of PG in the uterus can be a key factor for controlling the duration of labor. Since the synthesis of PGs in the uterus is catalyzed by cyclooxygenase-2 (COX-2), devising a tool to regulate the expression of COX-2 could provide a method for treating complicated labor. In this study, Sp-6 acupuncture treatment was evaluated for its potential in controlling uterine motility. Immunohistochemical methods showed the COX-2 enzyme was primarily found in the endometrium and myometrium of rat uterus. COX-2 expression in these two locations were intensified by pregnancy, but reduced by acupuncture at the Sp-6 acupoint. Uterine motility monitored during Sp-6 acupuncture was reduced by 28.15% (p < 0.05) and 19.88% (p < 0.05) in pregnant rats and non-pregnant rats, respectively. The significant reduction of uterine motility in pregnant rat suggests a role for Sp-6 acupuncture in regulating the expression of COX-2 during pregnancy. These results suggest that Sp-6 acupuncture could be used as a complementary method for controlling labor in human pregnancy.

Pancreatic morphology in pregnant rats exposed to DuP-697 - the irreversible, highly selective cyclooxygenase-2 inhibitor

2008 ◽  
Vol 63 (1) ◽  
pp. 96-101
Author(s):  
Franciszek Burdan ◽  
Justyna Szumiło ◽  
Jarosław Dudka ◽  
Agnieszka Korobowicz ◽  
Agnieszka Fronczek ◽  
...  
Keyword(s):  
Cyclooxygenase 2 ◽  
Pregnant Rats ◽  
Pancreatic Morphology

Gastrointestinal and hepatic toxicity of selective and non-selective cyclooxygenase-2 inhibitors in pregnant and non-pregnant rats

2004 ◽  
Vol 50 (5) ◽  
pp. 533-543 ◽  
Author(s):  
Franciszek Burdan ◽  
Justyna Szumilo ◽  
Robert Klepacz ◽  
Jaroslaw Dudka ◽  
Agnieszka Korobowicz ◽  
...  
Keyword(s):  
Cyclooxygenase 2 ◽  
Hepatic Toxicity ◽  
Pregnant Rats ◽  
Cyclooxygenase 2 Inhibitors

scholarly journals Hyperglycaemia in pregnant rats causes sex-related vascular dysfunction in adult offspring: role of cyclooxygenase-2

2017 ◽  
Vol 102 (8) ◽  
pp. 1019-1036 ◽  
Author(s):  
Francine Gomes de Sá ◽  
Diego Barbosa de Queiroz ◽  
Fernanda Elizabethe Ramos-Alves ◽  
Juliana Santos-Rocha ◽  
Odair Alves da Silva ◽  
...  
Keyword(s):  
Vascular Dysfunction ◽  
Cyclooxygenase 2 ◽  
Adult Offspring ◽  
Pregnant Rats

scholarly journals Effects of Acupuncture on CCL2 and CXCL8 Expression and the Subset of uNK Cells in Rats with Embryo Implantation Failure

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Weina Gao ◽  
Xiao Tang ◽  
Zhenyan Chen ◽  
Yue Guo ◽  
Lijun Wang ◽  
...  
Keyword(s):  
Treatment Group ◽  
Group Treatment ◽  
Acupuncture Treatment ◽  
Embryo Implantation ◽  
Implantation Failure ◽  
Pregnant Rats ◽  
Failure Group ◽  
Uterine Natural Killer Cells ◽  
Unk Cells ◽  
Progestin Treatment

The present study was designed to investigate the efficacy and mechanism of acupuncture treatment on embryo implantation failure in rats. The pregnant rats were randomized into normal group (N), implantation failure group (M), acupuncture treatment group (A), and progestin treatment group (W). The embryo implantation failure model was established by mifepristone. Efficacy of acupuncture treatment was evaluated by the number of implanted embryos. The expression of CCL2 and CXCL8 and the subset of uterine natural killer cells in the endometrium were detected. We demonstrated that the number of implanted embryos was dramatically reduced after mifepristone (M group) treatment, while the acupuncture (A group) and progestin (W group) treatments significantly rescued impaired embryo implantation. The protein and mRNA expressions of CCL2 and CXCL8 were significantly reduced by mifepristone treatment, but the attenuated expression of CCL2 and CXCL8 was markedly reversed by acupuncture or progestin treatment. More importantly, acupuncture and progestin could markedly increase the subset of uNK cells in rats with embryo implantation failure. These evidences suggest that acupuncture is able to modulate the endometrial immune microenvironment and thus improve embryo implantation in pregnant rats, which provides solid experimental evidence for the curative effect of acupuncture treatment on infertility.

scholarly journals α-Tocopherol Potentiates the Cervical Resistance Decreasing Effects of COX Inhibitors in Pregnant Rats: The Putative Role of Cyclooxygenase-2 Inhibition

2018 ◽  
Vol 368 (2) ◽  
pp. 292-298 ◽  
Author(s):  
Anna Kothencz ◽  
Judit Hajagos-Tóth ◽  
Kálmán F. Szűcs ◽  
Annamária Schaffer ◽  
Róbert Gáspár
Keyword(s):  
Cyclooxygenase 2 ◽  
Pregnant Rats ◽  
Cox Inhibitors ◽  
Putative Role ◽  
Cervical Resistance

Attenuated fever in pregnant rats is associated with blunted syntheses of brain cyclooxygenase-2 and PGE2

2002 ◽  
Vol 283 (6) ◽  
pp. R1346-R1353 ◽  
Author(s):  
Kyoko Imai-Matsumura ◽  
Kiyoshi Matsumura ◽  
Akira Terao ◽  
Yasuyoshi Watanabe
Keyword(s):  
Endothelial Cells ◽  
Cyclooxygenase 2 ◽  
Female Rats ◽  
Brain Endothelial Cells ◽  
Term Pregnancy ◽  
Pregnant Rats ◽  
Near Term ◽  
Fever Response ◽  
Colonic Temperature ◽  
The Brain

Attenuation of fever occurs in pregnant animals. This study examined a hypothesis that brain production of PGE2, the final mediator of fever, is suppressed in pregnant animals. Near-term pregnant rats and age-matched nonpregnant female rats were injected with lipopolysaccharide (100 μg/kg) intraperitoneally. Four hours later, colonic temperature was measured, their cerebrospinal fluid (CSF) was sampled for PGE2 assay, and their brains were processed for immunohistochemistry of cyclooxygenase-2, an enzyme involved in PGE2 biosynthesis. In the pregnant rats, lipopolysaccharide injection resulted in significantly smaller elevations in both colonic temperature and CSF-PGE2 level than in nonpregnant rats. In the pregnant rats, lipopolysaccharide-induced cyclooxygenase-2 expression was blunted in terms of the number of positive cells. There was a significant correlation between PGE2 level in CSF and the number of cyclooxygenase-2-positive endothelial cells. These results suggest that suppressed PGE2 production in the brain is one cause for the attenuated fever response at near-term pregnancy and that this suppressed PGE2 production is due to the suppressed induction of cyclooxygenase-2 in brain endothelial cells.

scholarly journals Expression and Biological Activity of Parathyroid Hormone-Related Peptide in Pregnant Rat Uterine Artery: Any Role for 8-Iso-Prostaglandin F2α?

Endocrinology ◽  
2007 ◽  
Vol 149 (2) ◽  
pp. 626-633 ◽  
Author(s):  
Ferhat Meziani ◽  
Angela Tesse ◽  
Sandra Welsch ◽  
Hélène Kremer ◽  
Mariette Barthelmebs ◽  
...  
Keyword(s):  
Uterine Artery ◽  
Prostaglandin F2α ◽  
Cyclooxygenase 2 ◽  
Pcr Analysis ◽  
Pregnant Rats ◽  
Pregnant Rat ◽  
Uterine Arteries ◽  
Pthrp Expression ◽  
D 20 ◽  
Pthrp Receptor

PTHrP is produced in vessels and acts as a local modulator of tone. We recently reported that PTHrP(1–34) is able to induce vasorelaxation in rat uterine arteries, but in pregnancy, this response is blunted and becomes strictly endothelium dependent. The present study aimed to get insights into the mechanisms involved in these changes because the adaptation of uterine blood flow is essential for fetal development. On d 20 of gestation, RT-PCR analysis of uterine arteries showed that PTH/PTHrP receptor (PTH1R) mRNA expression was decreased, whereas that of PTHrP mRNA was increased. This was associated with a redistribution of the PTHrP/PTH1R system, with both PTH1R protein and PTHrP peptide becoming concentrated in the intimal layer of arteries from pregnant rats. On the other hand, the blunted vasorelaxation induced by PTHrP(1–34) in uterine arteries from pregnant rats was specifically restored by indomethacin and a specific cyclooxygenase-2 inhibitor, NS 398. This was associated with an increase in cyclooxygenase-2 expression and in 8-iso-prostaglandin F2α release when uterine arteries from pregnant rats were exposed to high levels of PTHrP(1–34). Most interestingly, 8-iso-prostaglandin F2α itself was able to increase PTHrP expression and reduce PTH1R expression in cultured rat aortic smooth muscle cells. These results suggest a local regulation of uterine artery functions by PTHrP during pregnancy resulting from PTH1R redistribution. Moreover, they shed light on a potential role of 8-iso-prostaglandin F2α.

scholarly journals Signaling Pathways Involved in Sphingosine Kinase Activation and Sphingosine-1-Phosphate Release in Rat Myometrium in Late Pregnancy: Role in the Induction of Cyclooxygenase 2

Endocrinology ◽  
2008 ◽  
Vol 149 (9) ◽  
pp. 4669-4679 ◽  
Author(s):  
Martin Serrano-Sanchez ◽  
Zahra Tanfin ◽  
Denis Leiber
Keyword(s):  
Primary Cultures ◽  
Sphingosine Kinase ◽  
Late Pregnancy ◽  
Cyclooxygenase 2 ◽  
Late Gestation ◽  
Pregnant Rats ◽  
Prolonged Incubation ◽  
Sphingosine 1 Phosphate ◽  
Gi Protein ◽  
D 12

We investigated the regulation of the sphingosine kinase (SphK)/sphingosine-1-phosphate (S1P) axis and its role during pregnancy in the rat myometrium. SphK1 and SphK2 were coexpressed in myometrium during gestation. The levels and activity of SphK1/2 were modest at midgestation (d 12), increased at d 19 and progressively declined to low at postpartum. Similar patterns were observed for the phosphorylation of ERK and protein kinase C (PKC). Inhibition of PKC and ERK reduced SphK1/2 activity. In late pregnancy, levels of cyclooxygenase 2 (COX2) increased in parallel to SphK levels. Using a pharmacological approach, we demonstrated that in primary cultures of myometrial cells from d-19 pregnant rats, induction of COX2 was mediated by 4β-phorbol 12,13-dibutyrate and IL-1β through sequential activation of PKC, ERK1/2, and SphK1. S1P produced by SphK1 was released in the medium. Addition of S1P, IL-1β or 4β-phorbol 12,13-dibutyrate enhanced COX2 levels via Gi protein. Interestingly, S1P was also released by myometrial tissues at late gestation. This event was dependent on PKC/ERK/SphK1. By contrast, in d-12 myometrial tissues, the release of S1P was markedly reduced in association with low levels of SphK1 and COX2. However, prolonged incubation of myometrium from midgestation led to the induction of COX2. This effect was blocked by SphK inhibitors, providing evidence of the close relationship between SphK activity and COX2 induction in rat myometrium. Overall, our findings provided insight into the physiological relevance of the SphK activation and S1P release in uterine smooth muscle during gestation.

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