Effects of Social Isolation on Ehrlich Tumor Growth and Tumor Leukocyte Infiltration in Mice: Evidence of Participation of the Submaxillary Salivary Gland

Ketoprofen, a nonsteroidal anti-inflammatory drug (NSAID) has proven to exert anti-inflammatory, anti-proliferative and anti-angiogenic activities in both neoplastic and non-neoplastic conditions. We investigated the effects of this compound on tumor development in Swiss mice previously inoculated with Ehrlich tumor cells. To carry out this study the solid tumor was obtained from cells of the ascites fluid of Ehrlich tumor re-suspended in physiological saline to give 2.5x106cells in 0.05mL. After tumor inoculation, the animals were separated into two groups (n = 10). The animals treated with ketoprofen 0.1µg/100µL/animal were injected intraperitoneally at intervals of 24h for 10 consecutive days. Animals from the control group received saline. At the end of the experiment the mice were killed and the tumor removed. We analyzed tumor growth, histomorphological and immunohistochemical characteristics for CDC47 (cellular proliferation marker) and for CD31 (blood vessel marker). Animals treated with the ketoprofen 0.1µg/100µL/animal showed lower tumor growth. The treatment did not significantly influence the size of the areas of cancer, inflammation, necrosis and hemorrhage. Moreover, lower rates of tumor cell proliferation were observed in animals treated with ketoprofen compared with the untreated control group. The participation of ketoprofen in controlling tumor malignant cell proliferation would open prospects for its use in clinical and antineoplasic therapy.

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Changes of Androstenone Concentrations in Saliva of Boars with Age

Animals ◽

10.3390/ani12020157 ◽

2022 ◽

Vol 12 (2) ◽

pp. 157

Author(s):

Kamila Pokorná ◽

Jaroslav Čítek ◽

Petr Doležal ◽

Martyna Małopolska ◽

Mirosłav Tyra ◽

...

Keyword(s):

Salivary Gland ◽

Total Concentration ◽

Boar Taint ◽

Gas Chromatography Mass Spectrometry ◽

Large White ◽

Gland Weight ◽

Submaxillary Salivary Gland ◽

Positive Correlations ◽

Effect Of Age ◽

A Current

With the increasing age of boars, the possibility of androstenone (5α-androst-16-en-3-one (AND), 5α-androst-16-en-3α-ol (α-AND), and 5α-androst-16-en-3β-ol (β-AND)) occurrence increases as well. The aim of this study was to evaluate concentrations of androstenone compounds in the saliva of boars concerning the age of animals. In total, 72 boars were evaluated (24 boars per replication). The effect of age (three different ages—152, 163, and 172 days) was observed, and (Landrace × Large White) × Pietrain genotypes were used. Chemical analysis of saliva samples was conducted by multidimensional gas chromatography/mass spectrometry (MDGC/MS). Salivary α-AND increased with age (p < 0.05), and positive correlations were found between age and submaxillary salivary gland weight (p < 0.05), age, and salivary AND concentration (p < 0.05), body weight and submaxillary salivary gland weight (p < 0.05), submaxillary salivary gland weight and salivary β-AND concentration (p < 0.05), as well as submaxillary salivary gland weight and total salivary 5α-androstenone (AND total) concentration (p < 0.001). Nowadays, animal welfare is becoming a more and more discussed topic, and pig breeding is not an exception. Specifically, the castration ban is a current issue, so it is very important to know as much as possible about compounds responsible for boar taint. Androstenone’s appearance in boars’ saliva could be one of the most important precursors for future early detection of boar taint.

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Cohabitation with a Sick Cage Mate: Effects on Ascitic Form of Ehrlich Tumor Growth and Macrophage Activity

NeuroImmunoModulation ◽

10.1159/000121284 ◽

2007 ◽

Vol 14 (6) ◽

pp. 297-303 ◽

Cited By ~ 11

Author(s):

Glaucie Jussilane Alves ◽

Luciana Vismari ◽

João Palermo-Neto

Keyword(s):

Tumor Growth ◽

Ehrlich Tumor ◽

Macrophage Activity

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The Ehrlich Tumor Induces Pain-Like Behavior in Mice: A Novel Model of Cancer Pain for Pathophysiological Studies and Pharmacological Screening

BioMed Research International ◽

10.1155/2013/624815 ◽

2013 ◽

Vol 2013 ◽

pp. 1-12 ◽

Cited By ~ 15

Author(s):

Cassia Calixto-Campos ◽

Ana C. Zarpelon ◽

Mab Corrêa ◽

Renato D. R. Cardoso ◽

Felipe A. Pinho-Ribeiro ◽

...

Keyword(s):

Tumor Growth ◽

Cancer Pain ◽

Tumor Cells ◽

Tricyclic Antidepressant ◽

Mechanical Hyperalgesia ◽

Cyclooxygenase Inhibitor ◽

Paw Edema ◽

Ehrlich Tumor ◽

Novel Model ◽

Ehrlich Tumor Cells

The Ehrlich tumor is a mammary adenocarcinoma of mice that can be developed in solid and ascitic forms depending on its administration in tissues or cavities, respectively. The present study investigates whether the subcutaneous plantar administration of the Ehrlich tumor cells induces pain-like behavior and initial pharmacological susceptibility characteristics. The Ehrlich tumor cells (1 × 104–107cells) induced dose-dependent mechanical hyperalgesia (electronic version of the von Frey filaments), paw edema/tumor growth (caliper), and flinches compared with the saline group between days 2 and 12. There was no difference between doses of cells regarding thermal hyperalgesia in the hot-plate test. Indomethacin (a cyclooxygenase inhibitor) and amitriptyline hydrochloride (a tricyclic antidepressant) treatments did not affect flinches or thermal and mechanical hyperalgesia. On the other hand, morphine (an opioid) inhibited the flinch behavior and the thermal and mechanical hyperalgesia. These effects of morphine on pain-like behavior were prevented by naloxone (an opioid receptor antagonist) treatment. None of the treatments affected paw edema/tumor growth. The results showed that, in addition to tumor growth, administration of the Ehrlich tumor cells may represent a novel model for the study of cancer pain, specially the pain that is susceptible to treatment with opioids, but not to cyclooxygenase inhibitor or to tricyclic antidepressant.

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