Expression of Creatine Kinase Isoenzyme Genes during Postnatal Development of Rat Brain Cerebrum: Evidence for Posttranscriptional Regulation

2003 ◽  
Vol 25 (6) ◽  
pp. 421-435 ◽  
Author(s):  
Wei Shen ◽  
Dianna Willis ◽  
Yanping Zhang ◽  
George R. Molloy
Keyword(s):  
Creatine Kinase ◽  
Rat Brain ◽  
Postnatal Development ◽  
Posttranscriptional Regulation ◽  
Creatine Kinase Isoenzyme

Transformation of creatine kinase-BB isoenzyme in vitro: effect of carboxylic acids, thiols, pH, cations, and chelators.

1982 ◽  
Vol 28 (12) ◽  
pp. 2414-2417 ◽  
Author(s):  
J A Lott ◽  
J W Heinz
Keyword(s):  
Creatine Kinase ◽  
Rat Brain ◽  
Carboxylic Acids ◽  
Alkaline Ph ◽  
Creatine Kinase Isoenzyme ◽  
Creatine Kinase Bb ◽  
Vitro Effect ◽  
Dialyzed Serum

Abstract Creatine kinase isoenzyme BB from rat brain was incubated with serum, dialyzed serum, or plasma, with added carboxylic acids, thiols, buffers, or cations. It was found to be very unstable at 37 degrees C under all these conditions, being transformed to CK-BB', a form that migrates like CK-MB on agarose electrophoretic plates. This transformation is enhanced at alkaline pH and, especially, by Zn2+. CK-BB' is quite stable, and probably results from binding of cations to CK-BB, because the transformation is prevented by EDTA and citrate.

scholarly journals Expression of creatine kinase isoenzyme genes during postnatal development of rat brain cerebellum: evidence for transcriptional regulation

2002 ◽  
Vol 367 (2) ◽  
pp. 369-380 ◽  
Author(s):  
Wei SHEN ◽  
Dianna WILLIS ◽  
Yanping ZHANG ◽  
Uwe SCHLATTNER ◽  
Theo WALLIMANN ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Rat Brain ◽  
Postnatal Development ◽  
Time Course ◽  
Basic Protein ◽  
Cerebellar Development ◽  
Mrna Accumulation ◽  
The Many ◽  
The Brain ◽  
Enhancer Factor

Transcription and accumulation of brain-type creatine kinase (CKB) mRNA and its protein was examined during postnatal development of rat brain cerebellum, the brain region containing highest CKB mRNA in the adult. CKB protein was extremely low at day 1, increased about 10-fold until week 4 and remained constant until week 10. This time course was paralleled by cerebellar CKB mRNA, which was also extremely low at day 1 and increased 5-fold during the first 3 weeks and then remained constant. High levels of CKB protein were also detected in cultured primary cerebellar granular neurons. Nuclear run-on assays directly showed that CKB mRNA accumulation during postnatal cerebellar development was due to increased transcription. When compared with cerebrum and whole brain, cerebellar CKB mRNA accumulation during postnatal development was temporally delayed. Analysis of myocyte enhancer factor (MEF)-2 and Sp1, factors known to initiate or sustain CKB transcription in tissues other than brain, revealed that MEF-2 in cerebellum was low at week 1 but increased 3.5-fold by week 7, while Sp1 remained unchanged. The increase in CKB protein during cerebellar postnatal development was coincident with that of the ubiquitous mitochondrial CK protein and mRNA, indicating that a functional phosphocreatine energy shuttle probably exists for efficient ATP regeneration in the cerebellum. This should be beneficial for the many energy-demanding requirements during cerebellar development, as indicated by the observed temporal co-expression of CKB with myelin basic protein, which is involved in axon myelination by oligodendrocytes.

scholarly journals CARDIOPROTECTIVE EFFECT OF MENTHA LONGIFOLIA AGAINST CYCLOPHOSPHAMIDE INDUCED CARDIOTOXICITY IN RATS: A BIOCHEMICAL, ELECTROCARDIOGRAPHIC AND HISTOPATHOLOGICAL STUDY

2016 ◽  
Vol 8 (9) ◽  
pp. 214 ◽  
Author(s):  
Ayshath Afroos Shahana A.r. ◽  
Sanjiv Karale ◽  
Jagadish V. Kamath
Keyword(s):  
Creatine Kinase ◽  
Histopathological Examination ◽  
Myocardial Damage ◽  
Cardioprotective Effect ◽  
Cardiac Biomarkers ◽  
Oxidative Enzymes ◽  
Histopathological Study ◽  
Marker Enzyme ◽  
Creatine Kinase Isoenzyme ◽  
Mentha Longifolia

<p><strong>Objective: </strong>The current research was designed to evaluate the cardioprotective activity of <em>Mentha longifolia</em> (ML) leaf extract on cyclophosphamide-induced cardiotoxicity in rats.</p><p><strong>Methods: </strong>Cardiotoxicity was induced in <em>Albino wistar</em> rats of either sex by administering a single injection of cyclophosphamide (200 mg/kg, i. p.) on the first day of the experimental period. <em>Mentha longifolia</em> (250 and 500 mg/kg, p. o.) was administered daily for 10 d immediately after administration of cyclophosphamide on the first day. The general observations such as oxidative marker enzyme assays, ECG and histopathology were examined.</p><p><strong>Results: </strong>Cyclophosphamide administration significantly (p&lt;0.05) increased lipid peroxidation (LPO) and decreased the levels of antioxidant markers such as superoxide dismutase (SOD) and catalase (CAT). Cyclophosphamide elevated the levels of biomarker enzymes like creatine kinase isoenzyme MB (CK-MB), creatine kinase isoenzyme NAC (CK-NAC) and lactate dehydrogenase (LDH). Further, the cyclophosphamide-treated rats showed changes in electrocardiographic parameters. Treatment with <em>Mentha longifolia</em> significantly (p&lt;0.05) reversed the status of cardiac biomarkers, ECG and oxidative enzymes in cyclophosphamide-induced cardiotoxicity. Histopathological examination was also supported the potential cardioprotective effect of <em>Mentha longifolia</em> with reduced damage to the myocardium.</p><p><strong>Conclusion: </strong>The biochemical, ECG and histopathology reports support the potential benefits of <em>Mentha longifolia</em> against myocardial damage which could be attributed to antioxidant activity.</p>

FGF-18 is a neuron-derived glial cell growth factor expressed in the rat brain during early postnatal development

2002 ◽  
Vol 105 (1-2) ◽  
pp. 60-66 ◽  
Author(s):  
Masamitsu Hoshikawa ◽  
Akiko Yonamine ◽  
Morichika Konishi ◽  
Nobuyuki Itoh
Keyword(s):  
Growth Factor ◽  
Cell Growth ◽  
Rat Brain ◽  
Postnatal Development ◽  
Glial Cell ◽  
Early Postnatal Development

Creatine Kinase Isoenzyme DB in Cerebrospinal Fluid from Patients with Meningitis and Encephalitis

1983 ◽  
Vol 148 (1) ◽  
pp. 180-180 ◽  
Author(s):  
H. Briem ◽  
L. Lindquist ◽  
P. Lundbergh ◽  
E. Sego ◽  
B. Skoldenberg
Keyword(s):  
Cerebrospinal Fluid ◽  
Creatine Kinase ◽  
Creatine Kinase Isoenzyme
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