Reversed Phase HPLC Analysis of Proenkephalin-Related and Prodynorphin-Related End-Products in the Brain of a Urodele Amphibian, Ambystoma tigrinum

1993 ◽  
Vol 42 (2) ◽  
pp. 69-76 ◽  
Author(s):  
Robert M. Dores ◽  
Lorraine K. McDonald ◽  
Laura C. Purdom ◽  
Catherine A. Sei
Keyword(s):  
Hplc Analysis ◽  
Reversed Phase ◽  
Ambystoma Tigrinum ◽  
Reversed Phase Hplc ◽  
End Products ◽  
The Brain

scholarly journals Novel fish hypothalamic neuropeptides stimulate the release of gonadotrophins and growth hormone from the pituitary of sockeye salmon

2006 ◽  
Vol 188 (3) ◽  
pp. 417-423 ◽  
Author(s):  
Masafumi Amano ◽  
Shunsuke Moriyama ◽  
Masayuki Iigo ◽  
Shoji Kitamura ◽  
Noriko Amiya ◽  
...  
Keyword(s):  
Anterior Pituitary ◽  
Sockeye Salmon ◽  
Brain Regions ◽  
Pituitary Hormones ◽  
Reversed Phase ◽  
Enzyme Linked Immunosorbent Assay ◽  
Pituitary Cells ◽  
Reversed Phase Hplc ◽  
Hypothalamic Neuropeptides ◽  
The Brain

We recently identified a cDNA encoding three novel fish hypothalamic neuropeptides, having LPXRF-NH2 from the goldfish brain. In this study, to clarify the physiological functions of these three LPXRFamide peptides (gfLPXRFa-1, -2, and -3), we analysed the localisation and hypophysiotrophic activity of these peptides using sockeye salmon, Oncorhynchus nerka, in which immunoassay systems for several anterior pituitary hormones have been developed. gfLPXRFa-immunoreactive cell bodies were detected in the nucleus posterioris periventricularis of the hypothalamus and immunoreactive fibres were distributed in various brain regions and the pituitary. We also detected gfLPXRFa-immunoreactivity in the pituitary by competitive enzyme-linked immunosorbent assay combined with reversed-phase HPLC. These three gfLPXRFamide peptides stimulated the release of FSH, LH and GH, but did not affect the release of prolactin (PRL) and somatolactin (SL) from cultured pituitary cells. These results suggest that novel fish hypothalamic LPXR-Famide peptides exist in the brain and pituitary of sockeye salmon and stimulate the release of gonadotrophins and GH from the pituitary.

Practical, stable standards for the reversed phase HPLC analysis of peptidic leukotrienes

1987 ◽  
Vol 28 (7) ◽  
pp. 739-740 ◽  
Author(s):  
E.J. Corey ◽  
Stephen W. Wright ◽  
Mukund M. Mehrotra
Keyword(s):  
Hplc Analysis ◽  
Reversed Phase ◽  
Reversed Phase Hplc

Quantitative reversed phase HPLC analysis of L-ascorbic acid (Vitamin C) and identification of its degradation products

1987 ◽  
Vol 24 (1) ◽  
pp. 371-376 ◽  
Author(s):  
Linda L. Lloyd ◽  
F. P. Warner ◽  
C. A. White ◽  
J. F. Kennedy
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Hplc Analysis ◽  
Degradation Products ◽  
Reversed Phase ◽  
Reversed Phase Hplc

scholarly journals Reversed Phase HPLC Analysis of Component Fatty Acids in Lipophilic Components Extracted from Vinegar

2005 ◽  
Vol 52 (9) ◽  
pp. 412-419 ◽  
Author(s):  
Masahiro Fujimori ◽  
Tutomu Masuda ◽  
Keita Yunoki ◽  
Noritaka Kasikawa ◽  
Yoshinori Tsukamoto ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Hplc Analysis ◽  
Reversed Phase ◽  
Reversed Phase Hplc
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